RU2762200C1 - Method of feeding poultry with the introduction of an additive based on microorganisms of the genus bacillus into the feed - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture and namely to the way of feeding poultry. The method is characterized in that an additive based on microorganisms of the genus Bacillus and an additive from a mixture of citric and lactic acids, salts of formic acid: calcium formate and sodium formate are introduced into the feed, and the components are taken in a certain ratio. For the preparation of the supplement, two strains of bacteria of the genus Bacillus are used: the bacterial strain Bacillus mucilaginosus B-4901 and the bacterial strain Bacillus subtilis 1-85, consisting of viable spores of spore-forming bacteria with a total number of bacteria of at least 1*10⁶ CFU/g. In this case, citric and lactic acids, calcium formate and sodium formate, as well as the dried biomass of these strains are taken at the rate of 1.0 kg/ ton of feed.

EFFECT: use of the invention will improve feed quality.

1 cl, 1 ex, 1 tbl

Description

The invention relates to agriculture, in particular to a method of feeding poultry when using in the composition of feed additives with probiotic activity "Probiocid ^® -Ultra" from two strains of bacteria: Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 and additionally additives from the mixture: citric and lactic acids, salts of formic acid - calcium formate and sodium formate. The introduction of the proposed combination into the composition of the feed provides the possibility of replacing antibiotics when feeding poultry.

Known combined probiotic preparation for use in animal husbandry, containing a mixture of biomass of bacterial strains Bacillus subtilis VKM-B-2998D and Bacillus licheniformis VKM-B-2999D and as additives - biomass of the strain Bacillus subtilis (natto) VKM B-3057D and calcium gluconate, RU No. 2675934 C2, C12N 1/20, A61K 35/742, 25.12.2018.

There is a known method of feeding broiler chickens, including feeding, starting from day old until slaughter, a complete feed, to which a feed additive with probiotic activity is added, previously prepared by mixing a complex of bacterial strains in the form of a dry powder with a filler, RU # 2604501 C1, A23K 50/75, A23K 10/16, A23K 20/163, A23K 20/28, 10.12.2016.

A known method of growing poultry, including the introduction into the diet of young poultry feed additives to feed, starting from the age of two days, consisting of biomass and / or culture fluid obtained by cultivating the fungus Medusomyces Gisevii Lindau in a mixture with the probiotic "Bifidobacterin", RU No. 2574691 C1, A23K 1/16, 10.02.2016.

Known additive with probiotic activity on a mineral basis, used in the composition of feed for farm animals and poultry and consisting of spore-forming bacteria strain Bacillus subtilis 111, while the feed additive in the feed is taken at a ratio of 0.001: 1, RU No. 2569002 C1, A23K 1 / 16, A23K 1/175, 20.11.2015.

Known feed product for farm animals and birds, containing in its composition lactic acid, succinic acid, citric acid, sorption material and additionally dried biomass Lactobacillus acidophilus, Enterococcus faecium, Bacillus subtilis, Bacillus polymyxa, RU No. 2552872 C1, A23K 1/16, 10.06.2015.

Known biologically active complex for poultry and a method of its use, which consists in introducing a probiotic into the feed in the form of a complex of bacteria, including, inter alia, the culture liquid of bacteria of the genus Bacillus, and a dry biologically active additive - dry biomass of the fungus of the genus Fusarium, RU No. 2538116 C2, A23K 1/16, 01/10/2015.

Known strains of Bacillus subtilis with a high level of phytase production, on the basis of which a composition for feeding animals is obtained in combination with other additives, RU No. 2506307 C2, C12N1 / 20, A23K 1/16, 10.02.2014.

A known method of growing layers of parental herd, including the introduction of a probiotic feed additive "GidroLaktiV" into the diet, starting from 10 days of age to 21 days of development, while "GidroLaktiV" is administered dry at a dose of 0.7% of the mass of feed, RU No. 2503250 C2, A23K 1/16, A23K 1/08, 01/10/2014.

A known method of feeding a parent herd of poultry, including feeding a powdery feed additive, which is a substrate mycelium of the basidiomycete Canoderma lucidum, RU No. 2501292 C2, A23K 1/00, A23K 1/16, 20.12.2013.

There is a known method of growing replacement chickens for parental herd of chickens, including the introduction of probiotics into the diet by drinking the probiotic preparation Monosporin, and in the first phase of early productivity, the probiotic preparation Bacell is introduced into the diet of replacement chickens, RU No. 2495588 C2, A23K 1/165, 20.10.2013 ...

A known biological product with probiotic activity to optimize the assimilation of feed for farm animals and poultry, containing the microbial mass of spore-forming bacteria of the bacterial strain of the Bacillus cereus strain 1-79, RU No. 2493723 C1, A23K 1/00, A23K 1/165, C12N 1/20, 27.09 .2013.

It is known to use a bacterial strain Bacillus subtilis for the preparation of animal feed and feed additives, RU No. 2433738 C1, A23K 1/00, 20.11.2011; RU No. 2422506 C1, C12N1 / 00, A23K 1/00, A23K 1/16, A23K 1/175, 27.06.2011.

Known feed additive for broiler chickens and laying hens and method of its use, providing for feeding with compound feed (during the entire period of rearing young stock and keeping adult birds) feed additive, including methionine, sodium sulfate, vitamin E and filler, RU No. 2402922 C2 , A23K 1/16, A23K 1/175, 10.11.2010.

Known probiotic feed additive in the form of a mixture of microorganisms selected from the group Enterococcus faecium, DSM 16211, Lactobacillus reuteri, DSM 16350, Lactobacillus salivarius ssp. salivarius, DSM 16351, Pediococcus acidilactici, DSM 16210, Bifidobacterium animalis, DSM 16284, allowing to prevent the harmful action of microbes in the digestive system of poultry, RU No. 2372788 C2, A23K 1/00, A23K 1/18, 20.11.2009.

It is known to use an organic acid or its salt containing at least two carbon atoms as a preservative for plant material used for animal feed, RU No. 2366275 C2, A23K 3/03, 10.09.2009.

Known feed additive to increase productivity when raising farm animals, containing, among other components, organic acids (citric, fumaric, succinic, malic, etc.) or their salts, RU No. 2292157 C2, A23K 1/00, A23K 1/16, 27.01. 2007.

There is a method of growing poultry, including the introduction into the normal diet of young poultry of a feed additive containing a complex of biologically active substances in the form of biomass and / or culture fluid obtained by cultivating a strain of the fungus Fusarium sambucinum VKPM No. F-867 mixed with probiotic Avilakt-1K, RU No. 2286066 C2, A23K 1/16, A23K 1/165, 27.10.2006.

Known biologically active additive to feed for farm animals and poultry, containing a preparation with probiotic activity and organic acids, a preparation with probiotic activity which consists of biomass of strains of bacteria of the genus Bacillus, and among other organic acids contains citric acid, RU No. 2265366 C2, A23K 1 / 16, A23K 1/165, C12N 1/20, 10.12.2005.

A known method of feeding chickens, providing for the introduction into the feed of the consortium of bacteria VNIISHM 1-33, including Ruminococcus albus 1-33 and Lactobacillus acidophilus 1-33 in a ratio of 1: 1, RU No. 2171037 C1, A23K 1/165, C12N 1/20, 27.07 .2001.

In the known methods of feeding poultry and in the known feed additives used for feeding, no signs similar to the claimed invention have been found.

A known method of feeding poultry with the introduction of an additive to the feed based on microorganisms of the genus Bacillus, previously prepared, RU No. 2458526 C1, A23K 1/16, C12N 1/20, 20.08.2012.

This technical solution is taken as the closest analogue of the present invention.

The feed additive of the closest analogue contains viable spores of spore-forming bacteria Bacillus subtilis VKPM V-2574, Bacillus cereus VKPM V-2492, Bacillus licheniformis B-020, filler and dispersant.

There is no other composition of the feed additive in the closest analogue.

The basis of the present invention is the solution to the problem of increasing the bacterial activity and quality of feed when feeding poultry.

The technical result of the present invention consists in the introduction into the feed of an additive based on microorganisms of the genus Bacillus in the form of two strains of bacteria of the genus Bacillus: a bacterial strain Bacillus mucilaginosus B-4901 and a bacterial strain Bacillus subtilis 1-85, in improving the quality of feed, improving the digestibility and normalization of gastrointestinal microflora. - the intestinal tract of poultry, due to the use in the composition of an additive of a mixture of citric and lactic acids, salts of formic acid - calcium formate and sodium formate, in the possibility of replacing antibiotics proposed by the combination: the addition of bacterial strains Bacillus mucilaginosus B-4901 and Bacillus subtilis 1- 85, the addition of a mixture of citric and lactic acids, salts of formic acid - calcium formate and sodium formate.

According to the invention, this problem is solved due to the fact that the method of feeding poultry with the introduction of an additive based on microorganisms of the genus Bacillus into the feed. Pre-cooked additive.

To prepare the additive, two strains of bacteria of the genus Bacillus are used: the bacterial strain Bacillus mucilaginosus B-4901 and the bacterial strain Bacillus subtilis 1-85, consisting of viable spores of spore-forming bacteria, 0.1% for each bacterial strain Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 with a total number of bacteria of at least 1 * 10 ⁶ CFU / g.

In addition to the feed, an additive is added from a mixture of organic acids: citric and lactic acids in the amount of 51% - 39% and 13% - 17%, respectively, and a mixture of formic acid salts: calcium formate and sodium formate in the amount of 18% - 22% of each salt.

Moreover, citric acid, lactic acid, calcium formate and sodium formate are ground to a powder combined with dried biomass of bacterial strains Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 in a mixture in equal amounts.

In this case, citric and lactic acids, calcium formate and sodium formate, as well as the dried biomass of these strains are taken at the rate of 1.0 kg / ton of feed, providing feed with bactericidal properties that do not require the use of antibiotics.

The applicant has not identified sources containing information on technical solutions identical to the present invention, which allows us to conclude that it meets the criterion of "novelty".

Due to the implementation of the distinctive features of the invention (in conjunction with the features indicated in the limiting part of the claims), important new properties of the object are achieved.

The introduction of the dried biomass of the bacterial strains Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 into the feed increases its quality and probiotic activity, ensures the safety of young poultry and an increase in live weight.

The use of an additive from a mixture of organic acids: citric and lactic acids and a mixture of salts of formic acid: calcium formate and sodium formate provides an increase in the quality of feed, which improves the digestibility and normalization of the microflora of the gastrointestinal tract of poultry.

The possibility of introducing into the feed in the aggregate the dried biomass of the bacterial strains Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 and additives from a mixture of citric and lactic acids, salts of formic acid: calcium formate and sodium formate provides a replacement for the use of antibiotics.

The applicant is not aware of any publications that would contain information about the influence of the distinctive features of the invention on the achieved technical result. In this regard, according to the applicant, it can be concluded that the proposed technical solution meets the criterion of "inventive step".

The present invention is carried out as follows.

A method of feeding poultry with the introduction of an additive based on microorganisms of the genus Bacillus into the feed. Pre-cooked additive.

To prepare the additive, two strains of bacteria of the genus Bacillus are used: the bacterial strain Bacillus mucilaginosus B-4901 and the bacterial strain Bacillus subtilis 1-85, consisting of viable spores of spore-forming bacteria, 0.1% for each bacterial strain Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 with a total number of bacteria of at least 1 * 10 ⁶ CFU / g.

The bacterial strain Bacillus mucilaginosus B-4901 and the bacterial strain Bacillus subtilis 1-85 were deposited at the National Bioresource Center of the All-Russian collection of industrial microorganisms of the National Research Center "Kurchatov Institute" - State Research Institute of Genetics and in the All-Russian State Collection of Microorganism Strains Used in Veterinary Medicine and Animal Breeding numbers B-4901 and 02.08.05-DEP, respectively.

The bacterial strain Bacillus mucilaginosus B-4901 and the bacterial strain Bacillus subtilis 1-85 are stored in the collection of microorganisms of OOO BIOTROF.

In addition to the feed, an additive is added from a mixture of organic acids: citric and lactic acids in the amount of 51% - 39% and 13% - 17%, respectively, and a mixture of formic acid salts: calcium formate and sodium formate in the amount of 18% - 22% of each salt ...

Moreover, citric acid, lactic acid, calcium formate and sodium formate are ground to a powder combined with dried biomass of bacterial strains Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 in a mixture in equal amounts.

In this case, citric and lactic acids, calcium formate and sodium formate, as well as the dried biomass of these strains are taken at the rate of 1.0 kg / ton of feed, providing feed with bactericidal properties that do not require the use of antibiotics.

Research on the implementation of the proposed method is presented in the Example.

Production experience in replacing feed antibiotics in industrial poultry farming

The experiment was carried out in April-June 2020 at an intensive-type broiler poultry farm located in the Northwestern District of the Russian Federation.

The experiment was carried out on broilers of the Cobb-500 cross.

An additive based on bacterial strains Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 and an additive from a mixture of citric and lactic acids, salts of calcium formate and sodium formate were introduced into the feed and feeding was carried out starting from day old until slaughter (40 days) ...

The norms of keeping, feeding, drinking, stocking density, temperature and humidity were in accordance with the instructions for broilers of the Cobb-500 cross.

Two groups were formed:

- Control group - the main diet with the inclusion of the feed antibiotic Multimycin at a dosage of 0.3 kg / ton of feed.

- Experimental group - the main diet with the inclusion of an additive based on bacterial strains Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 and additives from a mixture of citric and lactic acids, calcium formate salts and sodium formate without the inclusion of a feed antibiotic at a dosage of 1.0 kg / tons of feed.

From the table, the possibility of replacing the antibiotic is obvious.

The example confirms the feasibility of introducing the proposed composition into the feed for agricultural poultry instead of an antibiotic during feeding.

Currently, the feed industry is in demand for drugs that can replace feed antibiotics. These include preparations with probiotic activity based on strains of microorganisms and acidifying preparations based on organic acids and their salts.

Formic acid has the shortest carbon chain of all acids, which explains its high antibacterial activity. However, preparations based on pure formic acid are corrosive and have a pungent odor. At the same time, salts of formic acid are characterized by very high antibacterial properties, especially calcium formate.

Citric acid (at room temperature is a dry powder) significantly improves the palatability of the feed and can increase its consumption.

Lactic acid is highly effective against E. coli and Salmonella.

The effectiveness of the use of feed preparations with probiotic activity based on strains of bacteria of the genus Bacillus, due to their manufacturability, showed a great ability to colonize the gastrointestinal tract, since bacterial spores are resistant to the acidic environment of the stomach and digestive enzymes (pepsin and trypsin).

In connection with the urgency of the problem of replacing feed antibiotics, it is advisable to use the proposed method of feeding poultry.

The proposed "Method for feeding poultry with the introduction of an additive based on microorganisms of the genus Bacillus into the feed" using an additive with probiotic activity and additives from a mixture of acids and salts obtained by known biotechnologies in the feed composition, in the applicant's opinion, determine the compliance of the proposed method with the criterion " industrial applicability ".

The proposed "Method for feeding poultry with the introduction of an additive based on microorganisms of the genus Bacillus into the feed" makes it possible to increase the bacterial activity and quality of feed when feeding poultry.

Claims (1)

A method of feeding poultry with the introduction of an additive based on microorganisms of the genus Bacillus, previously prepared, into the feed, characterized in that two strains of bacteria of the genus Bacillus are used for the preparation of the additive: a bacterial strain Bacillus mucilaginosus B-4901 and a bacterial strain Bacillus subtilis 1-85, consisting of viable spores of spore-forming bacteria, 0.1% for each bacterial strain Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 with a total number of bacteria of at least 1 * 10 ⁶ CFU / g, in addition to the feed add an additive from a mixture of organic acids: citric and lactic acids in the amount of 51% - 39% and 13% - 17%, respectively, and a mixture of salts of formic acid: calcium formate and sodium formate in the amount of 18% - 22% of each salt, and citric acid, lactic acid, calcium formate and sodium formate are ground to powder combined with dried biomass of bacterial strains Bacillus mucilaginosus B-4901 and Bacillus subtilis 1-85 in a mixture of equal quantities, with citric and lactic acids, calcium formate and sodium formate, as well as the dried biomass of these strains are taken at the rate of 1.0 kg / ton of feed, providing the feed with bactericidal properties that do not require the use of antibiotics.