RU2762197C1 - Method of feeding poultry with the introduction of a probiotic feed additive - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture and namely to the way of feeding poultry. The method includes introducing an additive with probiotic activity into the feed, previously prepared by mixing a complex of two strains: a bacterial strain Bacillus megaterium B-4801 and a bacterial strain Enterococcus faecium 1-35, applied to a filler, and additives from a mixture of organic acids: citric and lactic acids of the mixture and salts of formic acid: calcium formate and sodium formate, taken in a certain ratio and ground to a powder, as well as additives with phytobiotic activity containing a mixture of two natural essential oils: "lemongrass" and "oregano", which are applied to crystalline citric acid. The feed additive with probiotic activity is taken at the rate of 0.5 kg / ton of feed, and the additive from citric acid, lactic acid, calcium formate and sodium formate and the additive with phytobiotic activity is taken at the rate of 1.0 kg / ton of feed.

EFFECT: use of the invention will increase the bacterial activity and quality of feed.

1 cl, 1 tbl, 1 ex

Description

The invention relates to agriculture, in particular to a method of feeding poultry, starting from one day old and throughout the entire growing period, when used in the composition of the feed dry feed additive with probiotic activity "Profort" containing viable bacteria of the Enterococcus faecium 1-35 strain and viable bacteria of the Bacillus megaterium B-4801 strain, additionally additives from a mixture of citric and lactic acids, salts of calcium formate and sodium formate, and additionally an additive with phytobiotic activity from a mixture of natural essential oils "lemongrass" and "oregano". The introduction of the proposed combination into the composition of the feed provides the possibility of replacing antibiotics when feeding poultry.

Known combined probiotic preparation for use in animal husbandry, containing a mixture of biomass of bacterial strains Bacillus subtilis BKM-B-2998D and Bacillus licheniformis VKM-B-2999D in spore form in a ratio of 1: 1 and as additives - biomass of the strain Bacillus subtilis (natto) VKM B-3057D and calcium gluconate, RU No. 2675934 C2, C12N 1/20, A61K 35/742, 25.12.2018.

A known method of growing poultry, including the introduction into the diet of young poultry feed additives to feed, starting from the age of two days, consisting of biomass and / or culture fluid obtained by cultivating the fungus Medusomyces Gisevii Lindau in a mixture with the probiotic "Bifidobacterin", RU No. 2574691 C1, A23K 1/16, 10.02.2016.

Known feed additive with phytoprobiotic activity on a mineral basis, used in the composition of feed for farm animals and poultry and consisting of viable bacteria of the Enterococcus faecium1-35 strain with a bacterial titer of at least 10 ⁶ CFU / mg, plant materials and diatomite in the form of burnt crumbs, RU No. 2574689 C1, A23K 1/16, 02/10/2016.

Known feed additive with phytobiotic activity on a mineral basis, used in the composition of feed for farm animals and poultry and containing a mixture of essential oils of eucalyptus, thyme, garlic and lemon in the form of a dry powder, applied to diatomite, RU No. 2569018 C1, A23K 1/16, A23K 1/175, 20.11.2015.

Known feed product for farm animals and poultry, containing in its composition lactic acid, succinic acid, citric acid, sorption material in the form of a mixture of vermiculite and bentonite clay in the ratio (40:60) - (20:80) and additionally dried biomass Lactobacillus acidophilus , Enterococcus faecium, Bacillus subtilis, Bacillus polymyxa, RU # 2552872 C1, A23K 1/16, 10.06.2015.

A known method of feeding a parent herd of poultry, including feeding a powdery feed additive, which is a substrate mycelium of the basidiomycete Canoderma lucidum, obtained by hydrolysis to obtain a substrate and adding a liquid inoculum of the fungus Ganoderma lucidum, growing mycelium for drying and grinding 0.1% of the mass of the ration to the parent poultry flock, RU No. 2501292 C2, A23K 1/00, A23K 1/16, 20.12.2013.

A known method of growing replacement chickens for parental herd of chickens, including the introduction of probiotics into the diet, by drinking the probiotic preparation Monosporin at the rate of 3 ml per 100 heads per day for 10 days - this is in the starting period, and from the pre-laying period and in the first phase of early productivity Replacement young animals are introduced into the diet with the probiotic preparation Bacell in an amount of 0.2% of the mass of compound feed, RU No. 2495588 C2, A23K 1/165, 20.10.2013.

A known feed additive composition containing a preparation with phytobiotic activity and organic acids, the preparation with phytobiotic activity of which includes essential oil, and among other organic acids contains citric acid, RU No. 2484641 C2, A23K 1/16, 20.06.2013.

A known method of obtaining a biological product for feeding farm animals and poultry, containing in its composition a mixture of essential oils of garlic, eucalyptus, rosemary and thyme, RU No. 2458527 C1, A23K 1/165, 20.08.2012.

Known composition of feed additive for feeding farm animals, including 2-hydroxy-1-methylthiobutanoic acid and essential oil, RU No. 2420090 C2, A23K 1/16, 10.06.2011.

Known feed additive for broiler chickens and laying hens and a method of its use, providing for feeding with compound feed during the entire period of growing young stock and keeping adult poultry feed additives, including methionine, sodium sulfate, vitamin E and filler, RU No. 2402922 C2, A23K 1/16, A23K 1/175, 10.11.2010.

It is known to use an organic acid or its salt containing at least two carbon atoms as a preservative for plant material used for animal feed, RU No. 2366275 C2, A23K 3/03, 10.09.2009.

Known feed additive for increasing the resistance and productivity of farm animals and poultry, containing essential oil of vegetable raw materials and filler, RU No. 2297775 C1, A23K 1/16, A23K 1/14, 27.04.2007.

Known feed additive to increase productivity when raising farm animals, containing, among other components, organic acids (citric, fumaric, succinic, malic, etc.) or their salts, RU No. 2292157 C2, A23K 1/00, A23K 1/16, 27.01. 2007.

There is a method of growing poultry, including the introduction into a normal diet of young poultry of a feed additive containing a complex of biologically active substances in the form of biomass and / or culture liquid obtained by cultivating a strain of the fungus Fusarium sambucinum VKPM No. F-867 mixed with probiotic Avilakt-1K, RU No. 2286066 C2, A23K 1/16, A23K 1/165, 27.10.2006.

Known biologically active additive to feed for farm animals and poultry, containing a preparation with probiotic activity and organic acids, a preparation with probiotic activity which consists of biomass of strains of bacteria of the genus Bacillus, and among other organic acids contains citric acid, RU No. 2265366 C2, A23K 1 / 16, A23K 1/165, C12N 1/20, 10.12.2005.

In the known methods of feeding poultry and in the known feed additives used for feeding, no signs similar to the claimed invention have been found.

A known method of feeding poultry, including feeding, starting from one day of age and before slaughter, a complete feed, to which a feed additive with probiotic activity is added, previously prepared by mixing a complex of two strains: a bacillus strain Bacillus megaterium B-4801 and a bacterial strain Enterococcus faecium 1 -35, the dried biomass of which in a mixture in equal amounts is applied to a filler and contains live bacteria Enterococcus faecium 1-35 2.2 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 2.1 × 10 ⁸ CFU in 1 g of feed additive , RU No. 2652832 C1, A23K 10/75, A23K 10/18, 03.05.2018.

This technical solution is taken as the closest analogue of the present invention.

The method of feeding poultry of the closest analogue consists in feeding a complete feed with a known feed additive with probiotic activity.

There are no other additives in the closest analogue.

The present invention is based on the solution of the problem, which makes it possible to provide feeding of poultry with food with increased bacterial activity and high quality.

The technical result of the present invention is to provide a wide spectrum of probiotic action when using a feed additive, to increase the phytobiotic effect and to actively suppress opportunistic and pathogenic microflora, due to the introduction of natural essential oils "lemongrass" and "oregano" into the additive composition, in improving the quality of feed, improving the digestibility and normalization of the microflora of the gastrointestinal tract of poultry, due to the use in the composition of the additive from a mixture of citric and lactic acids, salts of formic acid - calcium formate and sodium formate, in the possibility of replacing antibiotics proposed by the combination: feed additive with probiotic activity "Profort", the addition of a mixture of "lemongrass" and "oregano" and the addition of a mixture of citric and lactic acids, salts of formic acid - calcium formate and sodium formate.

According to the invention, this problem is solved due to the fact that the method of feeding poultry with the introduction of a probiotic feed additive includes feeding, starting from day old until slaughter, a complete feed, to which a feed additive with probiotic activity is added, previously prepared by mixing a complex of two strains: bacterial strain Bacillus megaterium B-4801 and bacterial strain Enterococcus faecium 1-35. Dried biomass of strains of bacteria Bacillus megaterium B-4801 and Enterococcus faecium 1-35 in a mixture in equal amounts applied to a filler and contains live bacteria Enterococcus faecium 1-35 2.2 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 2.1 × 10 ⁸ CFU in 1 g of feed additive.

In addition to the feed, an additive is added from a mixture of organic acids: citric and lactic acids in the amount of 51% -39% and 13% -17%, respectively, and a mixture of formic acid salts: calcium formate and sodium formate in the amount of 18% -22% of each salt ...

Moreover, citric acid, lactic acid, calcium formate and sodium formate are ground to powder.

Also, in addition to the feed, an additive with phytobiotic activity is added.

Supplement with phytobiotic activity contains a mixture of two natural essential oils: "lemongrass" and "oregano" in a 1: 1 ratio of 0.1% each.

Natural essential oils "lemongrass" and "oregano" are applied to crystalline citric acid in a ratio of 1: 2, respectively.

Crystalline citric acid with a mixture of "lemongrass" and "oregano" is added to the powder of citric acid, lactic acid, calcium formate and sodium formate in an amount of 0.3% by weight of the finished additive and stirred in a mixer until smooth.

A feed additive with probiotic activity, an additive from a mixture of citric and lactic acids, salts of calcium formate and sodium formate, as well as an additive in the form of a phytobiotic activity preparation from a mixture of natural essential oils "lemongrass" and "oregano" are used in combination when feeding.

In this case, the feed additive with probiotic activity is taken at the rate of 0.5 kg / ton of feed, and the additive from citric acid, lactic acid, calcium formate and sodium formate and the additive with phytobiotic activity is taken at the rate of 1.0 kg / ton of feed, providing feed bactericidal properties that do not require the use of antibiotics.

The applicant has not identified sources containing information on technical solutions identical to the present invention, which allows us to conclude that it meets the "novelty" criterion.

Due to the implementation of the distinctive features of the invention (in conjunction with the features indicated in the limiting part of the claims), important new properties of the object are achieved.

The introduction of a dry feed additive based on the Bacillus megaterium B-4801 bacterial strain and the Enterococcus faecium 1-35 bacterial strain into the feed increases its quality and probiotic activity, ensures the safety of young poultry and an increase in live weight during feeding during the entire growing period.

The use of an additional additive from a mixture of organic acids: citric and lactic acids and a mixture of formic acid salts: calcium formate and sodium formate provides an increase in the quality of feed, which improves the digestibility and normalization of the microflora of the gastrointestinal tract of poultry.

The use of an additional additive from a mixture of a preparation of phytobiotic activity of natural essential oils "lemongrass" and "oregano" provides an increase in the phytobiotic effect and active suppression of opportunistic and pathogenic microflora.

The possibility of introducing a feed additive with probiotic activity into the feed, an additive in the form of a phytobiotic activity preparation from a mixture of natural essential oils "lemongrass" and "oregano" and the addition of a mixture of citric and lactic acids, formic acid salts - calcium formate and sodium formate provides a replacement for use antibiotics.

The applicant is not aware of any publications that would contain information about the influence of the distinctive features of the invention on the achieved technical result. In this regard, according to the applicant, it can be concluded that the proposed technical solution meets the criterion of "inventive step".

The present invention is carried out as follows.

The method of feeding poultry with the introduction of a probiotic feed additive includes feeding, starting from day old to slaughter, a complete feed.

A feed additive with probiotic activity is added to the feed, previously prepared by mixing a complex of two strains: the Bacillus megaterium B-4801 bacterial strain and the Enterococcus faecium 1-35 bacterial strain. Dried biomass of strains of bacteria Bacillus megaterium B-4801 and Enterococcus faecium 1-35 in a mixture in equal amounts applied to a filler and contains live bacteria Enterococcus faecium 1-35 2.2 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 2.1 × 10 ⁸ CFU in 1 g of feed additive.

The bacterial strain Enterococcus faecium 1-35 was deposited in the collection of the All-Russian State Research Institute for Control, Standardization and Certification of Veterinary Medicines (VGNKI) on March 20, 2008 under the registration number Enterococcus faecium VGNKI 08.03.53-DEP and is stored in the collection of microorganisms of OOO BIOTROF.

The bacterial strain Bacillus megaterium B-4801 was deposited in the All-Russian Collection of Industrial Microorganisms of the Federal State Unitary Enterprise GosNII Genetics under the registration number VKPM: B-4801 and is stored in the collection of microorganisms of OOO BIOTROF.

Experimental samples of cultures of strains of bacteria Enterococcus faecium 1-35 and Bacillus megaterium B-4801 have been produced in fermenters.

To obtain a feed additive with probiotic activity, the dried biomass of the Enterococcus faecium 1-35 bacterial strain and the Bacillus megaterium B-4801 bacterial strain was taken.

The dried biomasses of these strains are applied in a mixture in equal amounts to a filler.

The quantitative content of living bacteria has been determined experimentally and is optimal.

The feed additive with probiotic activity is ground to a powder.

In addition to the feed, an additive is added from a mixture of organic acids: citric and lactic acids in the amount of 51% -39% and 13% -17%, respectively, and a mixture of formic acid salts: calcium formate and sodium formate in the amount of 18% -22% of each salt ...

Moreover, citric acid, lactic acid, calcium formate and sodium formate are ground to powder.

Also, in addition to the feed, an additive with phytobiotic activity is added.

Supplement with phytobiotic activity contains a mixture of two natural essential oils: "lemongrass" and "oregano" in a 1: 1 ratio of 0.1% each.

Natural essential oils "lemongrass" and "oregano" are applied to crystalline citric acid in a ratio of 1: 2, respectively.

Crystalline citric acid with a mixture of "lemongrass" and "oregano" is added to the powder of citric acid, lactic acid, calcium formate and sodium formate in an amount of 0.3% by weight of the finished additive and stirred in a mixer until smooth.

A feed additive with probiotic activity, an additive from a mixture of citric and lactic acids, salts of calcium formate and sodium formate, as well as an additive in the form of a phytobiotic activity preparation from a mixture of natural essential oils "lemongrass" and "oregano" are used in combination when feeding.

In this case, the feed additive with probiotic activity is taken at the rate of 0.5 kg / ton of feed, and the additive from citric acid, lactic acid, calcium formate and sodium formate and the additive with phytobiotic activity is taken at the rate of 1.0 kg / ton of feed, providing feed bactericidal properties that do not require the use of antibiotics.

Research on the implementation of the proposed method is presented in the Example.

Production experience in replacing feed antibiotics in industrial poultry farming

The experiment was carried out in April-June 2020 at an intensive-type broiler poultry farm located in the Northwestern District of the Russian Federation.

The experiment was carried out on broilers of the Cobb-500 cross.

The feed additive with probiotic activity "Profort", an additive from a mixture of citric and lactic acids, salts of calcium formate and sodium formate, an additive from a mixture of natural essential oils "lemongrass" and "oregano" in total were introduced into the feed and the feeding was carried out starting from the daily age and before slaughter (40 days).

The norms of keeping, feeding, drinking, stocking density, temperature and humidity were in accordance with the instructions for broilers of the Cobb-500 cross.

Two groups were formed:

- Control group - the main diet with the inclusion of the feed antibiotic Multimycin at a dosage of 300 g per ton of compound feed.

- Experimental group - the main diet with the inclusion of a feed additive with probiotic activity (dosage 0.5 kg / ton of feed) "Profort", additives from a mixture of citric and lactic acids, calcium formate salts and sodium formate and additives from a mixture of natural essential oils "lemongrass "And" oregano "(dosage 1.0 kg / ton of feed), without the inclusion of feed antibiotic.

The results of cultivation are presented in the table.

From the table, the possibility of replacing the antibiotic is obvious.

The example confirms the feasibility of introducing the proposed composition into the feed for agricultural poultry instead of an antibiotic when feeding from day old and throughout the entire growing period.

Currently, the feed industry is in demand for drugs that can replace feed antibiotics. These include drugs with pro-probiotic activity, acidifying drugs, drugs with phytoprobiotic activity. Of great interest is the combination of acidifying preparations based on organic acids and their salts and preparations with pro-probiotic activity based on microorganism strains that have a beneficial effect on the productivity of poultry.

Acidifying agents, when added to poultry feed, activate appetite and are a readily available source of energy.

Formic acid has the shortest carbon chain of all acids, which explains its high antibacterial activity. However, preparations based on pure formic acid are corrosive and have a pungent odor. In addition, the salts of formic acid have the best performance of the gastrointestinal tract, and calcium formate is characterized by very high antibacterial properties.

Citric acid significantly improves the palatability of the feed, while the birds increase feed intake.

Lactic acid shows high efficiency in the fight against Escherichia coli and Salmonella, improves the taste of feed.

The effectiveness of the use of feed preparations with pro-probiotic activity based on bacteria of the genus Bacillus, due to their manufacturability, showed a great ability to colonize the gastrointestinal tract, since bacterial spores are resistant to the acidic environment of the stomach and digestive enzymes (pepsin and trypsin).

In connection with the urgency of the problem of replacing feed antibiotics, it is advisable to use the proposed method of feeding poultry.

The proposed "Method for feeding poultry with the introduction of a probiotic feed additive" using in the composition of the feed dry feed additive with high probiotic activity "Profort", obtained by known biotechnologies, as well as additives from a mixture of acids and salts and from a mixture of natural essential oils having application in agriculture, and the conducted studies of the use of the method, determine, in the opinion of the applicant, compliance with the criterion of "industrial applicability".

The proposed "Method for feeding poultry with the introduction of a probiotic feed additive" allows feeding poultry with food with increased bacterial activity and high quality. The food in the proposed method has bactericidal properties that do not require the use of antibiotics.

Claims (1)

A method of feeding poultry with the introduction of a probiotic feed additive, including feeding, from one day of age to slaughter, a complete feed, to which a feed additive with probiotic activity is added, previously prepared by mixing a complex of two strains: a bacillus strain Bacillus megaterium B-4801 and a strain bacteria Enterococcus faecium 1-35, the dried biomass of which in a mixture in equal amounts is applied to a filler and contains live bacteria Enterococcus faecium 1-35 2.2 × 10 ⁸ CFU and live bacteria Bacillus megaterium B-4801 2.1 × 10 ⁸ CFU in 1 g of feed additive, characterized in that, in addition to the feed, an additive is added from a mixture of organic acids: citric and lactic acids in an amount of 51-39% and 13-17%, respectively, and a mixture of formic acid salts: calcium formate and sodium formate in an amount 18-22% of each salt, with citric acid, lactic acid, calcium formate and sodium formate ground to a powder, and in addition to the feed, an additive with phytobiotic activity is added, containing a mixture of two natural essential oils: "lemongrass" and "oregano" in a ratio of 1: 1, 0.1% each, which are applied to crystalline citric acid in a ratio of 1: 2, respectively, and crystalline citric acid with a mixture of "lemongrass" and "oregano" was added to the powder of citric acid, lactic acid, calcium formate and sodium formate in an amount of 0.3% by weight of the finished additive and stirred in a mixer until homogeneous, in addition, a feed additive with probiotic activity , an additive from a mixture of citric and lactic acids, salts of calcium formate and sodium formate, as well as an additive in the form of a preparation of phytobiotic activity from a mixture of natural essential oils "lemongrass" and "oregano" is used in combination when feeding, while the feed additive with probiotic activity is taken at the rate of 0.5 kg / ton of feed, and an additive from citric acid, lactic acid, calcium formate and sodium formate and add taste with phytobiotic activity is taken at the rate of 1.0 kg / ton of feed, providing feed with bactericidal properties that do not require the use of antibiotics.