RU2741806C2 - Устройство и способы для культивирования клеток - Google Patents
Устройство и способы для культивирования клеток Download PDFInfo
- Publication number
- RU2741806C2 RU2741806C2 RU2019100603A RU2019100603A RU2741806C2 RU 2741806 C2 RU2741806 C2 RU 2741806C2 RU 2019100603 A RU2019100603 A RU 2019100603A RU 2019100603 A RU2019100603 A RU 2019100603A RU 2741806 C2 RU2741806 C2 RU 2741806C2
- Authority
- RU
- Russia
- Prior art keywords
- gel
- cells
- microfluidic
- capillary pressure
- microfluidic channel
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 84
- 238000012258 culturing Methods 0.000 title claims abstract description 21
- 210000004027 cell Anatomy 0.000 claims abstract description 269
- 230000004888 barrier function Effects 0.000 claims abstract description 171
- 210000002220 organoid Anatomy 0.000 claims abstract description 150
- 239000012530 fluid Substances 0.000 claims abstract description 92
- 210000002889 endothelial cell Anatomy 0.000 claims abstract description 80
- 210000004088 microvessel Anatomy 0.000 claims abstract description 25
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 24
- 239000011248 coating agent Substances 0.000 claims abstract description 19
- 238000000576 coating method Methods 0.000 claims abstract description 19
- 238000011068 loading method Methods 0.000 claims abstract description 6
- 239000010410 layer Substances 0.000 claims description 112
- 210000001519 tissue Anatomy 0.000 claims description 99
- 238000004113 cell culture Methods 0.000 claims description 86
- 210000003463 organelle Anatomy 0.000 claims description 59
- 239000002243 precursor Substances 0.000 claims description 52
- 230000033115 angiogenesis Effects 0.000 claims description 36
- 239000000463 material Substances 0.000 claims description 30
- 230000001023 pro-angiogenic effect Effects 0.000 claims description 30
- 230000002792 vascular Effects 0.000 claims description 25
- 238000004891 communication Methods 0.000 claims description 24
- 150000001875 compounds Chemical class 0.000 claims description 23
- 230000000717 retained effect Effects 0.000 claims description 19
- 206010028980 Neoplasm Diseases 0.000 claims description 11
- 230000001939 inductive effect Effects 0.000 claims description 8
- 230000014759 maintenance of location Effects 0.000 claims description 8
- 210000000056 organ Anatomy 0.000 claims description 8
- 238000001574 biopsy Methods 0.000 claims description 5
- 239000002975 chemoattractant Substances 0.000 claims description 5
- 239000002356 single layer Substances 0.000 claims description 4
- 230000004936 stimulating effect Effects 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- 239000000126 substance Substances 0.000 abstract description 12
- 230000010412 perfusion Effects 0.000 abstract description 9
- 239000006285 cell suspension Substances 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 3
- 230000026341 positive regulation of angiogenesis Effects 0.000 abstract 1
- 238000000859 sublimation Methods 0.000 abstract 1
- 230000008022 sublimation Effects 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 242
- 239000000203 mixture Substances 0.000 description 66
- 210000002744 extracellular matrix Anatomy 0.000 description 53
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 48
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 48
- 239000007788 liquid Substances 0.000 description 30
- 239000002609 medium Substances 0.000 description 28
- 238000003556 assay Methods 0.000 description 26
- 210000005166 vasculature Anatomy 0.000 description 24
- 239000000017 hydrogel Substances 0.000 description 17
- 239000001963 growth medium Substances 0.000 description 16
- 210000003734 kidney Anatomy 0.000 description 15
- 108010082117 matrigel Proteins 0.000 description 15
- 239000003795 chemical substances by application Substances 0.000 description 13
- 210000002919 epithelial cell Anatomy 0.000 description 12
- DUYSYHSSBDVJSM-KRWOKUGFSA-N sphingosine 1-phosphate Chemical compound CCCCCCCCCCCCC\C=C\[C@@H](O)[C@@H](N)COP(O)(O)=O DUYSYHSSBDVJSM-KRWOKUGFSA-N 0.000 description 12
- 108010035532 Collagen Proteins 0.000 description 11
- 102000008186 Collagen Human genes 0.000 description 11
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 11
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 11
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 11
- 229920001436 collagen Polymers 0.000 description 11
- 239000000758 substrate Substances 0.000 description 11
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 9
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 9
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 9
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 9
- PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 description 9
- 230000035755 proliferation Effects 0.000 description 9
- 101710155857 C-C motif chemokine 2 Proteins 0.000 description 8
- 102000000018 Chemokine CCL2 Human genes 0.000 description 8
- 239000006143 cell culture medium Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 7
- 108050007372 Fibroblast Growth Factor Proteins 0.000 description 7
- 239000002870 angiogenesis inducing agent Substances 0.000 description 7
- 210000002469 basement membrane Anatomy 0.000 description 7
- 229940126864 fibroblast growth factor Drugs 0.000 description 7
- 238000011049 filling Methods 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 239000012085 test solution Substances 0.000 description 7
- 238000002054 transplantation Methods 0.000 description 7
- 102000012422 Collagen Type I Human genes 0.000 description 6
- 108010022452 Collagen Type I Proteins 0.000 description 6
- 230000002491 angiogenic effect Effects 0.000 description 6
- 230000004069 differentiation Effects 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 210000004185 liver Anatomy 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 239000011247 coating layer Substances 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 210000003038 endothelium Anatomy 0.000 description 5
- 230000002209 hydrophobic effect Effects 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 230000006698 induction Effects 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 238000012604 3D cell culture Methods 0.000 description 4
- 102100034594 Angiopoietin-1 Human genes 0.000 description 4
- 102100034608 Angiopoietin-2 Human genes 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000012136 culture method Methods 0.000 description 4
- 230000003511 endothelial effect Effects 0.000 description 4
- 230000035784 germination Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 210000002865 immune cell Anatomy 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- -1 polydimethylsiloxane Polymers 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 210000003491 skin Anatomy 0.000 description 4
- 230000000638 stimulation Effects 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 239000007995 HEPES buffer Substances 0.000 description 3
- 206010021143 Hypoxia Diseases 0.000 description 3
- 102000004372 Insulin-like growth factor binding protein 2 Human genes 0.000 description 3
- 108090000964 Insulin-like growth factor binding protein 2 Proteins 0.000 description 3
- 241001529936 Murinae Species 0.000 description 3
- 102100033237 Pro-epidermal growth factor Human genes 0.000 description 3
- 102000004079 Prolyl Hydroxylases Human genes 0.000 description 3
- 108010043005 Prolyl Hydroxylases Proteins 0.000 description 3
- 108010009583 Transforming Growth Factors Proteins 0.000 description 3
- 102000009618 Transforming Growth Factors Human genes 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000003501 co-culture Methods 0.000 description 3
- 238000012364 cultivation method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000011067 equilibration Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 239000012091 fetal bovine serum Substances 0.000 description 3
- 238000001879 gelation Methods 0.000 description 3
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 3
- 102000006495 integrins Human genes 0.000 description 3
- 108010044426 integrins Proteins 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 238000010899 nucleation Methods 0.000 description 3
- 230000008520 organization Effects 0.000 description 3
- 210000000496 pancreas Anatomy 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 210000001525 retina Anatomy 0.000 description 3
- 230000007480 spreading Effects 0.000 description 3
- 238000003892 spreading Methods 0.000 description 3
- 210000000130 stem cell Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- QGVLYPPODPLXMB-UBTYZVCOSA-N (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-4a,7b,9,9a-tetrahydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-1,1a,1b,4,4a,7a,7b,8,9,9a-decahydro-5H-cyclopropa[3,4]benzo[1,2-e]azulen-5-one Chemical compound C1=C(CO)C[C@]2(O)C(=O)C(C)=C[C@H]2[C@@]2(O)[C@H](C)[C@@H](O)[C@@]3(O)C(C)(C)[C@H]3[C@@H]21 QGVLYPPODPLXMB-UBTYZVCOSA-N 0.000 description 2
- 102000008076 Angiogenic Proteins Human genes 0.000 description 2
- 108010074415 Angiogenic Proteins Proteins 0.000 description 2
- 108010048154 Angiopoietin-1 Proteins 0.000 description 2
- 108010048036 Angiopoietin-2 Proteins 0.000 description 2
- 102000007350 Bone Morphogenetic Proteins Human genes 0.000 description 2
- 108010007726 Bone Morphogenetic Proteins Proteins 0.000 description 2
- 101800003838 Epidermal growth factor Proteins 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 108010067306 Fibronectins Proteins 0.000 description 2
- 102000016359 Fibronectins Human genes 0.000 description 2
- 101000924552 Homo sapiens Angiopoietin-1 Proteins 0.000 description 2
- 101000924533 Homo sapiens Angiopoietin-2 Proteins 0.000 description 2
- 108010085895 Laminin Proteins 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 108010067787 Proteoglycans Proteins 0.000 description 2
- 102000016611 Proteoglycans Human genes 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 239000012736 aqueous medium Substances 0.000 description 2
- 210000003719 b-lymphocyte Anatomy 0.000 description 2
- 229940112869 bone morphogenetic protein Drugs 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000000423 cell based assay Methods 0.000 description 2
- 230000024245 cell differentiation Effects 0.000 description 2
- 230000003915 cell function Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000002512 chemotherapy Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 210000004443 dendritic cell Anatomy 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 108060002566 ephrin Proteins 0.000 description 2
- 102000012803 ephrin Human genes 0.000 description 2
- 229940116977 epidermal growth factor Drugs 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000001747 exhibiting effect Effects 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 210000004209 hair Anatomy 0.000 description 2
- 210000003494 hepatocyte Anatomy 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 210000005075 mammary gland Anatomy 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000005499 meniscus Effects 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 210000001616 monocyte Anatomy 0.000 description 2
- 230000001338 necrotic effect Effects 0.000 description 2
- 239000005022 packaging material Substances 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- QGVLYPPODPLXMB-QXYKVGAMSA-N phorbol Natural products C[C@@H]1[C@@H](O)[C@]2(O)[C@H]([C@H]3C=C(CO)C[C@@]4(O)[C@H](C=C(C)C4=O)[C@@]13O)C2(C)C QGVLYPPODPLXMB-QXYKVGAMSA-N 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000007423 screening assay Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 210000004927 skin cell Anatomy 0.000 description 2
- 210000000603 stem cell niche Anatomy 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 210000002536 stromal cell Anatomy 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- 238000002723 toxicity assay Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 210000003606 umbilical vein Anatomy 0.000 description 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 2
- 230000008728 vascular permeability Effects 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- HJCMDXDYPOUFDY-WHFBIAKZSA-N Ala-Gln Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCC(N)=O HJCMDXDYPOUFDY-WHFBIAKZSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- AQGNHMOJWBZFQQ-UHFFFAOYSA-N CT 99021 Chemical compound CC1=CNC(C=2C(=NC(NCCNC=3N=CC(=CC=3)C#N)=NC=2)C=2C(=CC(Cl)=CC=2)Cl)=N1 AQGNHMOJWBZFQQ-UHFFFAOYSA-N 0.000 description 1
- 108010029240 Cell-Tak Proteins 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920002971 Heparan sulfate Polymers 0.000 description 1
- 241001441571 Hiodontidae Species 0.000 description 1
- 101000668058 Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99) RNA-directed RNA polymerase catalytic subunit Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102100037369 Nidogen-1 Human genes 0.000 description 1
- 102000004264 Osteopontin Human genes 0.000 description 1
- 108010081689 Osteopontin Proteins 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 108010067520 RADA16-I Proteins 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 108010031318 Vitronectin Proteins 0.000 description 1
- 102100035140 Vitronectin Human genes 0.000 description 1
- 102000013814 Wnt Human genes 0.000 description 1
- 108050003627 Wnt Proteins 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 210000000677 aggregate cell Anatomy 0.000 description 1
- 239000000556 agonist Substances 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 238000004135 animal tissue culture Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000000823 artificial membrane Substances 0.000 description 1
- 210000001130 astrocyte Anatomy 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 230000004641 brain development Effects 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 230000004656 cell transport Effects 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 230000003399 chemotactic effect Effects 0.000 description 1
- 229960005188 collagen Drugs 0.000 description 1
- 239000000512 collagen gel Substances 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000032459 dedifferentiation Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 238000003255 drug test Methods 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 238000004049 embossing Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000001842 enterocyte Anatomy 0.000 description 1
- 210000003158 enteroendocrine cell Anatomy 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 210000003238 esophagus Anatomy 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 210000001650 focal adhesion Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 210000002175 goblet cell Anatomy 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 230000001146 hypoxic effect Effects 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003715 interstitial flow Effects 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 230000029795 kidney development Effects 0.000 description 1
- 210000001039 kidney glomerulus Anatomy 0.000 description 1
- 210000001865 kupffer cell Anatomy 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000005073 lymphatic endothelial cell Anatomy 0.000 description 1
- 210000004324 lymphatic system Anatomy 0.000 description 1
- 210000001365 lymphatic vessel Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 208000004141 microcephaly Diseases 0.000 description 1
- 210000000274 microglia Anatomy 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 210000000651 myofibroblast Anatomy 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 108010008217 nidogen Proteins 0.000 description 1
- 102000045246 noggin Human genes 0.000 description 1
- 108700007229 noggin Proteins 0.000 description 1
- 210000004248 oligodendroglia Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 210000003668 pericyte Anatomy 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 230000008823 permeabilization Effects 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920002338 polyhydroxyethylmethacrylate Polymers 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 210000004129 prosencephalon Anatomy 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 210000001202 rhombencephalon Anatomy 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000003556 vascular endothelial cell Anatomy 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/069—Vascular Endothelial cells
- C12N5/0691—Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0858—Side walls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/28—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from vascular endothelial cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Cell Biology (AREA)
- Vascular Medicine (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2016965 | 2016-06-15 | ||
| NL2016965A NL2016965B1 (en) | 2016-06-15 | 2016-06-15 | Cell culture device. |
| NL2018033 | 2016-12-21 | ||
| NL2018033 | 2016-12-21 | ||
| PCT/EP2017/064302 WO2017216113A2 (en) | 2016-06-15 | 2017-06-12 | Cell culture device and methods |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2019100603A RU2019100603A (ru) | 2020-07-15 |
| RU2019100603A3 RU2019100603A3 (https=) | 2020-08-12 |
| RU2741806C2 true RU2741806C2 (ru) | 2021-01-28 |
Family
ID=59215734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2019100603A RU2741806C2 (ru) | 2016-06-15 | 2017-06-12 | Устройство и способы для культивирования клеток |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US11629319B2 (https=) |
| EP (1) | EP3472301B1 (https=) |
| JP (1) | JP6967535B2 (https=) |
| CN (1) | CN109804057B (https=) |
| AU (1) | AU2017286096B2 (https=) |
| BR (1) | BR112018075920A2 (https=) |
| CA (1) | CA3027616A1 (https=) |
| RU (1) | RU2741806C2 (https=) |
| WO (1) | WO2017216113A2 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2858716C1 (ru) * | 2024-12-26 | 2026-03-23 | Автономная некоммерческая образовательная организация высшего образования "Научно-технологический университет "Сириус" | Способ и устройство для дифференцировки мышечных клеток |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11760965B2 (en) | 2017-10-19 | 2023-09-19 | Georgia Tech Research Corporation | Mesofluidic device for culture of cell aggregates |
| NL2020518B1 (en) | 2018-03-02 | 2019-09-12 | Mimetas B V | Device and method for performing electrical measurements |
| CN108486035B (zh) * | 2018-03-26 | 2021-11-26 | 西北大学 | 一种三维类器官的液滴培养方法 |
| NL2022085B1 (en) * | 2018-11-28 | 2020-06-25 | Mimetas B V | Device for assessing mechanical strain induced in or by cells |
| KR102209346B1 (ko) * | 2019-03-13 | 2021-01-29 | 재단법인대구경북과학기술원 | 미세관통막 기반의 수화젤 마이크로웰 어레이 및 이를 이용한 3차원 단일세포 분리 및 배양방법 |
| US12024696B2 (en) * | 2019-05-08 | 2024-07-02 | Molecular Devices (Austria) GmbH | System and method for organoid culture |
| US20220238042A1 (en) * | 2019-05-10 | 2022-07-28 | Oregon Health & Science University | Engineered bone marrow model |
| JP2022534778A (ja) * | 2019-05-28 | 2022-08-03 | シリン・シェン | 患者由来ミクロオルガノスフェアのための方法及び装置 |
| KR102233992B1 (ko) * | 2019-06-28 | 2021-03-29 | 건양대학교 산학협력단 | 면역화학진단 세포 칩 구조체 |
| WO2021040874A1 (en) * | 2019-08-28 | 2021-03-04 | Massachusetts Institute Of Technology | In vitro tissue plate |
| CN110564674B (zh) * | 2019-09-29 | 2021-05-11 | 广东工业大学 | 一种干细胞铺底工作液及其应用 |
| EP4047082A4 (en) * | 2019-10-17 | 2023-08-16 | Public University Corporation Yokohama City University | METHOD OF EVALUATION OF TOXICITY OF DRUG |
| NL2024202B1 (en) * | 2019-11-08 | 2021-07-20 | Mimetas B V | Microfluidic cell culture system |
| US20230029554A1 (en) * | 2019-12-12 | 2023-02-02 | The Walter And Eliza Hall Institute Of Medecal Research | Organoid cultures |
| EP3907007A1 (de) | 2020-05-08 | 2021-11-10 | Technische Universität Wien | Mikrofluidische vorrichtung |
| US20210394177A1 (en) * | 2020-06-19 | 2021-12-23 | The Texas A&M University System | Systems and methods for mimicking a blood vessel of a patient |
| NL2026038B1 (en) * | 2020-07-09 | 2022-03-15 | Mimetas B V | Microfluidic cell culture device |
| CN112143642B (zh) * | 2020-08-28 | 2022-06-14 | 上海交通大学 | 用于体外培养的血管化肿瘤微流控器官芯片及其制备方法 |
| CA3246410A1 (en) | 2020-09-04 | 2025-10-31 | 9493662 Canada Inc. | Multi-well device, kits and methods for analysis of cells |
| EP3998332A4 (en) * | 2020-09-30 | 2022-09-28 | Beijing Daxiang Biotech Co., Ltd | 3D MULTIORGAN COCULTURE CHIP |
| JP7565008B2 (ja) * | 2020-10-07 | 2024-10-10 | ウシオ電機株式会社 | マイクロ流体デバイス及びマイクロ流体デバイスの使用方法 |
| CN116601287A (zh) * | 2020-10-22 | 2023-08-15 | 分子装置(奥地利)有限公司 | 用于自动化类器官培养的微孔板 |
| CN116457452A (zh) * | 2020-10-22 | 2023-07-18 | 得克萨斯大学体系董事会 | 高通量细胞迁移测定板及其制造方法 |
| CN112358966B (zh) * | 2020-10-23 | 2022-03-22 | 上海交通大学 | 一种滑动式微流控器官芯片及其制备方法 |
| CN117157147A (zh) * | 2020-12-01 | 2023-12-01 | 新加坡科技研究局 | 用于血管化细胞聚集体的装置和方法 |
| CN116981764A (zh) * | 2021-02-19 | 2023-10-31 | 分子装置(奥地利)有限公司 | 使用微孔板孔单元进行类器官传代的方法 |
| CA3211177A1 (en) * | 2021-03-16 | 2022-09-22 | Andrea PAVES | A well insert and a device for 3d cell culture and in vitro tissue model |
| EP4303297A1 (en) * | 2021-04-09 | 2024-01-10 | Cellartgen Inc. | Microfluidic hanging drop culture device for culturing cell aggregates |
| US12480079B2 (en) | 2021-04-23 | 2025-11-25 | Massachusetts Institute Of Technology | Fluidic platforms for perfusable vascularized tissues with infiltrates |
| CN113755328A (zh) * | 2021-09-08 | 2021-12-07 | 中山大学 | 一种类器官培养装置及类器官培养方法 |
| EP4423234A1 (en) * | 2021-10-29 | 2024-09-04 | Universität Bern | Microfluidic cell culturing device |
| USD1028280S1 (en) | 2021-12-27 | 2024-05-21 | Molecular Devices (Austria) GmbH | Organoid microplate |
| US12544754B2 (en) | 2022-01-18 | 2026-02-10 | Molecular Devices (Austria) GmbH | 3D printing of organoid passaging plate |
| WO2023144747A1 (en) * | 2022-01-28 | 2023-08-03 | Glaxosmithkline Intellectual Property Development Limited | High-throughput assay for cell migration, chemotaxis, and function |
| CN114591836A (zh) * | 2022-02-18 | 2022-06-07 | 清华大学深圳国际研究生院 | 体外血管床微流控芯片及其应用 |
| WO2023178320A1 (en) * | 2022-03-18 | 2023-09-21 | Molecular Devices (Austria) GmbH | Cell culture application methods of using a separation well microplate |
| JP2025509834A (ja) * | 2022-03-21 | 2025-04-11 | モレキュラー デバイシーズ （オーストリア） ゲーエムベーハー | マイクロプレートで共培養するためのシステムおよび方法 |
| WO2023218024A1 (en) * | 2022-05-13 | 2023-11-16 | Ecole Polytechnique Federale De Lausanne (Epfl) | System for automated and dynamic cell culture |
| US20230383225A1 (en) * | 2022-05-27 | 2023-11-30 | University Of Utah Research Foundation | Lung-on-chip device with integrated extracellular matrix membrane |
| DE102022209416B3 (de) * | 2022-09-09 | 2023-12-21 | Robert Bosch Gesellschaft mit beschränkter Haftung | Mikrofluidische Vorrichtung |
| WO2024129942A1 (en) * | 2022-12-14 | 2024-06-20 | President And Fellows Of Harvard College | Organoids in extracellular matrix droplets |
| DE102023200837A1 (de) * | 2023-02-02 | 2024-08-08 | Carl Zeiss Ag | Probengefäß zur Kultivierung biologischer Proben, Vorrichtung zu dessen Betrieb und Mikroskop |
| WO2024182622A1 (en) * | 2023-03-01 | 2024-09-06 | Research Institute At Nationwide Children's Hospital | Systems, devices, and processes for miniaturized physiological mimicry |
| NL2034328B1 (en) | 2023-03-13 | 2024-09-24 | Mimetas B V | Liver vasculature |
| WO2024191299A1 (en) | 2023-03-13 | 2024-09-19 | Mimetas B.V. | Liver vasculature |
| CN117586876B (zh) * | 2023-10-07 | 2024-10-25 | 丹望医疗科技(上海)有限公司 | 一种血管化类器官芯片及血管化类器官 |
| WO2025080829A1 (en) * | 2023-10-10 | 2025-04-17 | The Trustees Of The University Of Pennsylvania | A model of the blood-brain barrier on a chip |
| WO2025132381A1 (en) * | 2023-12-21 | 2025-06-26 | F. Hoffmann-La Roche Ag | Cell culturing device |
| WO2025186340A1 (de) * | 2024-03-05 | 2025-09-12 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | Vorrichtung zum erzeugen von drei-dimensionalen zellstrukturen in einem hydrogel, mikrosystem und/oder mikrofluidische zellkulturplattform und verfahren zum herstellen der vorrichtung |
| CN118374350A (zh) * | 2024-04-15 | 2024-07-23 | 丹望医疗科技(上海)有限公司 | 血管化类器官芯片及其应用 |
| CN118086053B (zh) * | 2024-04-29 | 2024-07-09 | 四川迪亚生物科技集团有限公司 | 一种自灌流培养板及使用方法 |
| TR2024007044A1 (tr) * | 2024-06-04 | 2025-12-22 | Izmir Yueksek Teknoloji Enstituesue Rektoerluegue | 2 ve 3 boyutlu hücre ve doku kültürünleri̇nde kullanilmak üzere üstü açik bi̇r mi̇krofi̇zyoloji̇k si̇stem ve bu si̇stemi̇n çalişma yöntemi̇ |
| GB202409293D0 (en) | 2024-06-27 | 2024-08-14 | Momo Biotech Ltd | Device |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012118799A2 (en) * | 2011-02-28 | 2012-09-07 | President And Fellows Of Harvard College | Cell culture system |
| WO2014038943A1 (en) * | 2012-09-10 | 2014-03-13 | Universiteit Leiden | Improvements relating to capillary pressure barriers |
| US20140335496A1 (en) * | 2011-12-05 | 2014-11-13 | Research Triangle Institute | Human conducting airway model comprising multiple fluidic pathways |
| WO2015032889A1 (en) * | 2013-09-05 | 2015-03-12 | Universität Bern | Device for in-vitro modelling in-vivo tissues of organs |
| WO2015138032A2 (en) * | 2013-12-20 | 2015-09-17 | President And Fellows Of Harvard College | Organomimetic devices and methods of use and manufacturing thereof |
| RU2584598C1 (ru) * | 2015-03-27 | 2016-05-20 | Общество с ограниченной ответственностью Научно-технический центр "БиоКлиникум" | Микрофлюидный чип для создания клеточных моделей органов млекопитающих |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4829000A (en) | 1985-08-30 | 1989-05-09 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Reconstituted basement membrane complex with biological activity |
| US7534601B2 (en) * | 2002-08-27 | 2009-05-19 | Vanderbilt University | Capillary perfused bioreactors with multiple chambers |
| GB0321158D0 (en) * | 2003-09-10 | 2003-10-08 | Central Research Lab Ltd | Apparatus and method for handling cells,embryos or oocytes |
| WO2007008609A2 (en) | 2005-07-07 | 2007-01-18 | The Regents Of The University Of California | Methods and apparatus for cell culture array |
| US20110129850A1 (en) | 2006-12-22 | 2011-06-02 | UCLA Office of Intellectual Property | Microfluidic platform for cell culture and assay |
| JP5343092B2 (ja) | 2008-01-29 | 2013-11-13 | カリフォルニア インスティチュート オブ テクノロジー | 細胞分離を行う精密濾過の方法及び装置 |
| US20110117634A1 (en) * | 2008-04-21 | 2011-05-19 | Asaf Halamish | Flat cell carriers with cell traps |
| WO2009146911A2 (en) | 2008-06-04 | 2009-12-10 | Uwe Marx | Organ-on-a-chip-device |
| CN104328050B (zh) * | 2008-07-16 | 2017-12-15 | 儿童医疗中心有限公司 | 具有微通道的器官模仿装置及其使用和制造方法 |
| WO2009118015A2 (en) * | 2008-08-01 | 2009-10-01 | Smart Biosystems Aps | A sample port of a cell culture system |
| GB0815427D0 (en) * | 2008-08-22 | 2008-10-01 | Capsant Neurotechnologies S A | Device |
| DK2342317T3 (da) | 2008-09-22 | 2013-03-18 | Univ Zurich Prorektorat Forschung | Hængende-dråbe-plade |
| WO2010062911A2 (en) | 2008-11-26 | 2010-06-03 | Hurel Corporation | Compositions and methods of functionally enhanced in vitro cell culture system |
| EP2213364A1 (en) | 2009-01-30 | 2010-08-04 | Albert-Ludwigs-Universität Freiburg | Phase guide patterns for liquid manipulation |
| JP2010253572A (ja) | 2009-04-21 | 2010-11-11 | Makita Corp | 切断機における割り刃の固定具脱落防止構造 |
| GB0910330D0 (en) | 2009-06-16 | 2009-07-29 | Univ Leiden | A biological microfluidics chip and related methods |
| US9261496B2 (en) | 2010-09-29 | 2016-02-16 | Massachusetts Institute Of Technology | Device for high throughput investigations of multi-cellular interactions |
| GB201103917D0 (en) | 2011-03-08 | 2011-04-20 | Univ Leiden | Apparatus for and methods of processing liquids or liquid based substances |
| US20140348706A1 (en) * | 2011-10-24 | 2014-11-27 | Agency For Science, Technology And Research | Microfluidic Device and Microfluidic System |
| WO2013134778A1 (en) * | 2012-03-09 | 2013-09-12 | Si-Bone Inc. | Impactor |
| CN106399094A (zh) | 2012-04-01 | 2017-02-15 | Emd密理博公司 | 细胞培养和梯度迁移化验方法和装置 |
| WO2014127250A1 (en) * | 2013-02-14 | 2014-08-21 | Cfd Research Corporation | Cell culture device with an array of microfluidic networks |
| EP2811012A1 (en) | 2013-06-07 | 2014-12-10 | Eidgenossisch Technische Hochschule Zürich | Hanging network plate |
| EP3218714A4 (en) | 2014-11-10 | 2018-07-04 | The Government of the United States of America as represented by the Secretary of the Navy | Modular microtube network for vascularized organ-on-a-chip models |
| WO2016081751A1 (en) | 2014-11-19 | 2016-05-26 | Nortis, Inc. | Method for vascularizing in-vitro generated or ex-vivo tissue fragments in a microfluidic device |
| CN104877905A (zh) | 2015-05-04 | 2015-09-02 | 清华大学深圳研究生院 | 细胞体外共培养微流控芯片、系统及方法 |
| NL2014840B1 (en) * | 2015-05-21 | 2017-01-31 | Intecrypt B V | Microfluidic device for in vitro 3D cell culture experimentation. |
| JP6640998B2 (ja) | 2015-06-05 | 2020-02-05 | ミメタス ビー．ブイ． | マイクロ流体プレート |
-
2017
- 2017-06-12 JP JP2018565659A patent/JP6967535B2/ja active Active
- 2017-06-12 EP EP17732821.8A patent/EP3472301B1/en active Active
- 2017-06-12 RU RU2019100603A patent/RU2741806C2/ru active
- 2017-06-12 AU AU2017286096A patent/AU2017286096B2/en active Active
- 2017-06-12 CN CN201780045603.5A patent/CN109804057B/zh active Active
- 2017-06-12 US US16/309,298 patent/US11629319B2/en active Active
- 2017-06-12 CA CA3027616A patent/CA3027616A1/en active Pending
- 2017-06-12 BR BR112018075920-1A patent/BR112018075920A2/pt not_active Application Discontinuation
- 2017-06-12 WO PCT/EP2017/064302 patent/WO2017216113A2/en not_active Ceased
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012118799A2 (en) * | 2011-02-28 | 2012-09-07 | President And Fellows Of Harvard College | Cell culture system |
| US20140335496A1 (en) * | 2011-12-05 | 2014-11-13 | Research Triangle Institute | Human conducting airway model comprising multiple fluidic pathways |
| WO2014038943A1 (en) * | 2012-09-10 | 2014-03-13 | Universiteit Leiden | Improvements relating to capillary pressure barriers |
| WO2015032889A1 (en) * | 2013-09-05 | 2015-03-12 | Universität Bern | Device for in-vitro modelling in-vivo tissues of organs |
| WO2015138032A2 (en) * | 2013-12-20 | 2015-09-17 | President And Fellows Of Harvard College | Organomimetic devices and methods of use and manufacturing thereof |
| RU2584598C1 (ru) * | 2015-03-27 | 2016-05-20 | Общество с ограниченной ответственностью Научно-технический центр "БиоКлиникум" | Микрофлюидный чип для создания клеточных моделей органов млекопитающих |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2858716C1 (ru) * | 2024-12-26 | 2026-03-23 | Автономная некоммерческая образовательная организация высшего образования "Научно-технологический университет "Сириус" | Способ и устройство для дифференцировки мышечных клеток |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2017286096B2 (en) | 2022-01-20 |
| CA3027616A1 (en) | 2017-12-21 |
| JP2019517808A (ja) | 2019-06-27 |
| EP3472301C0 (en) | 2023-08-02 |
| CN109804057A (zh) | 2019-05-24 |
| CN109804057B (zh) | 2022-09-09 |
| RU2019100603A (ru) | 2020-07-15 |
| WO2017216113A3 (en) | 2018-02-01 |
| EP3472301B1 (en) | 2023-08-02 |
| US20200063081A1 (en) | 2020-02-27 |
| BR112018075920A2 (pt) | 2019-03-26 |
| WO2017216113A2 (en) | 2017-12-21 |
| AU2017286096A1 (en) | 2019-01-17 |
| JP6967535B2 (ja) | 2021-11-17 |
| US11629319B2 (en) | 2023-04-18 |
| EP3472301A2 (en) | 2019-04-24 |
| RU2019100603A3 (https=) | 2020-08-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2741806C2 (ru) | Устройство и способы для культивирования клеток | |
| US11725173B2 (en) | Highly efficient organoid culture device and system | |
| Sodunke et al. | Micropatterns of Matrigel for three-dimensional epithelial cultures | |
| JP7588582B2 (ja) | 細胞内において又は細胞によって誘導された機械的歪みの評価のための装置 | |
| US9121847B2 (en) | Three-dimensional microfluidic platforms and methods of use thereof | |
| Wang et al. | In vitro generation of colonic epithelium from primary cells guided by microstructures | |
| US20250250521A1 (en) | Microphysiological organoid model | |
| EP4148117B1 (en) | CELL CULTURE DEVICE, CELL CULTURE PROCESSES USING IT, CELL CULTURE INCUBATOR INCLUDING IT | |
| US20110306110A1 (en) | Method for three-dimensional hierarchical cell co-culture | |
| AU2022289996B2 (en) | Method and apparatus for forming a microfluidic gel structure | |
| Nashimoto et al. | Perfusable vascular network with a tissue model in a microfluidic device | |
| US20230203417A1 (en) | Microfluidic device | |
| NL2016965B1 (en) | Cell culture device. | |
| WO2023036909A1 (en) | Cell culture apparatus, methods for cell cultivation by using the same, cell culture incubator comprising the same, and uses of the cell culture apparatus | |
| US20260117161A1 (en) | Cell culture apparatus, methods for cell cultivation by using the same, cell culture incubator comprising the same, and uses of the cell culture apparatus | |
| US20260109925A1 (en) | Microfluidic device for forming three-dimensional tissue barrier | |
| KR102807243B1 (ko) | 자동화장비 연동이 가능한 오가노이드 (또는 스페로이드) 배양용 마이크로 채널 | |
| KR20250164662A (ko) | 그래디언트 블록을 포함하는 조립형 마이크로어레이 플랫폼 및 이를 이용한 세포, 스페로이드 또는 오가노이드 어레이의 제조방법 | |
| CN117957303A (zh) | 细胞培养装置、使用该细胞培养装置的细胞培养方法、包括该细胞培养装置的细胞培养孵箱以及该细胞培养装置的用途 | |
| JP2026508594A (ja) | 肝臓血管系 | |
| WO2025202125A1 (en) | Cell culturing device | |
| Hacking et al. | Future approaches to organ regeneration: microscale environments, stem cell engineering, and self-assembly of living tissues | |
| IT202100025460A1 (it) | Dispositivo lab-on-chip per studiare la migrazione cellulare in sistemi tridimensionali e relativo metodo di utilizzo | |
| Fan | Cell Dynamics in Three-dimensional (3D) Culture Environments |