RU2730656C2 - Маломасштабный способ культивирования суспендированных клеток - Google Patents

Маломасштабный способ культивирования суспендированных клеток Download PDF

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RU2730656C2
RU2730656C2 RU2017140920A RU2017140920A RU2730656C2 RU 2730656 C2 RU2730656 C2 RU 2730656C2 RU 2017140920 A RU2017140920 A RU 2017140920A RU 2017140920 A RU2017140920 A RU 2017140920A RU 2730656 C2 RU2730656 C2 RU 2730656C2
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culture
cell
cells
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small
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RU2017140920A (ru
RU2017140920A3 (enExample
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Штеффен Голетц
Райнер ШТАН
Штеффен КРЕЙЕ
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Гликотоп Гмбх
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
RU2017140920A 2015-05-29 2016-05-25 Маломасштабный способ культивирования суспендированных клеток RU2730656C2 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
LU92727 2015-05-29
LU92727 2015-05-29
EP16151811.3 2016-01-19
EP16151811 2016-01-19
PCT/EP2016/061763 WO2016193083A1 (en) 2015-05-29 2016-05-25 Small scale cultivation method for suspension cells

Publications (3)

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RU2017140920A RU2017140920A (ru) 2019-07-01
RU2017140920A3 RU2017140920A3 (enExample) 2019-07-17
RU2730656C2 true RU2730656C2 (ru) 2020-08-24

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RU2017140920A RU2730656C2 (ru) 2015-05-29 2016-05-25 Маломасштабный способ культивирования суспендированных клеток

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US (1) US20180135091A1 (enExample)
EP (1) EP3303557B1 (enExample)
JP (1) JP6737813B2 (enExample)
KR (1) KR20180010200A (enExample)
CN (1) CN108138128A (enExample)
AU (1) AU2016272566A1 (enExample)
BR (1) BR112017023624A2 (enExample)
CA (1) CA2985435A1 (enExample)
RU (1) RU2730656C2 (enExample)
WO (1) WO2016193083A1 (enExample)

Families Citing this family (9)

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Publication number Priority date Publication date Assignee Title
CN108138128A (zh) 2015-05-29 2018-06-08 葛莱高托普有限公司 用于悬浮细胞的小规模养殖方法
US11713458B2 (en) 2016-06-20 2023-08-01 Octapharma Ag Means and methods for modifying multiple alleles
WO2018062358A1 (ja) * 2016-09-29 2018-04-05 Ｃｏａｉｄｏ株式会社 災害緊急用地域一斉架電システム
JP7317466B2 (ja) * 2017-12-12 2023-07-31 株式会社日立製作所 細胞株及び培養条件のスクリーニング方法、及びその装置
CN111139216B (zh) * 2018-11-01 2024-09-03 上海药明生物技术有限公司 高通量、自动化的细胞连续培养方法及其应用
EP3947632A1 (en) * 2019-04-01 2022-02-09 The Automation Partnership (Cambridge) Ltd. Operation process for a cell cultivation system
US20220328128A1 (en) * 2019-05-08 2022-10-13 Zymergen Inc. Downscaling parameters to design experiments and plate models for micro-organisms at small scale to improve prediction of performance at larger scale
CA3144960A1 (en) * 2019-07-01 2021-01-07 Ology Bioservices, Inc. Method for cultivation of adherent cells in a multiparallel bioreactor
CN118064651B (zh) * 2024-03-11 2025-03-11 上海迈邦生物科技有限公司 一种细胞灌流培养体系维持稳态的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0599651A2 (en) * 1992-11-25 1994-06-01 National Research Council Of Canada Bioreactor for the perfusion culture of cells
WO2012085162A1 (en) * 2010-12-21 2012-06-28 Glycotope Gmbh Methods for culturing human myeloid leukaemia cells and cells derived therefrom

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JP2997771B2 (ja) * 1998-06-29 2000-01-11 金沢大学長 細胞の懸濁培養方法
US20040185535A1 (en) 2003-03-21 2004-09-23 Giles Wilson Industrial-scale serum-free production of recombinant FVII in mammalian cells
US20040185534A1 (en) * 2000-10-02 2004-09-23 Knudsen Ida Molgaard Industrial-scale serum-free production of recombinant proteins in mammalian cells
WO2007071072A1 (en) 2005-12-22 2007-06-28 Corporation De L'ecole Polytechnique De Montreal High-rate perfusion bioreactor
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
EP2014760A1 (en) 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
DK2501822T3 (da) * 2009-11-17 2017-11-27 Squibb & Sons Llc Fremgangsmåder til forbedret proteinproduktion
EP2599651A1 (de) 2011-12-01 2013-06-05 Magna E-Car Systems GmbH & Co OG Heiz-/Kühlsystem für eine Batterie eines Kraftfahrzeugs sowie Betriebsverfahren hierfür
CN102690781B (zh) * 2012-04-28 2014-04-30 苏州金盟生物技术有限公司 一种灌流式细胞培养的培养基更换方法
CN104195187B (zh) 2014-08-01 2018-02-16 润科生物工程（福建）有限公司 沉降‑逆灌流‑放流偶联制备富含dha油脂的方法及其专用细胞沉降槽
CN108138128A (zh) 2015-05-29 2018-06-08 葛莱高托普有限公司 用于悬浮细胞的小规模养殖方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0599651A2 (en) * 1992-11-25 1994-06-01 National Research Council Of Canada Bioreactor for the perfusion culture of cells
WO2012085162A1 (en) * 2010-12-21 2012-06-28 Glycotope Gmbh Methods for culturing human myeloid leukaemia cells and cells derived therefrom

Non-Patent Citations (1)

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Title
WOODSIDE S.M., et al. "Mammalian cell retention devices for stirred perfusion bioreactors", Cytotechnology.1998; 28:163-175;. ГОРДЕЕВ А.А. "Методы скрининга живых клеток", Успехи биологической химии. 2018; 58:173-222. *

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Publication number Publication date
RU2017140920A (ru) 2019-07-01
WO2016193083A1 (en) 2016-12-08
AU2016272566A1 (en) 2017-11-23
US20180135091A1 (en) 2018-05-17
CA2985435A1 (en) 2016-12-08
KR20180010200A (ko) 2018-01-30
EP3303557A1 (en) 2018-04-11
BR112017023624A2 (pt) 2018-07-17
EP3303557B1 (en) 2021-05-05
JP2018516077A (ja) 2018-06-21
CN108138128A (zh) 2018-06-08
RU2017140920A3 (enExample) 2019-07-17
JP6737813B2 (ja) 2020-08-12

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