CN108138128A - 用于悬浮细胞的小规模养殖方法 - Google Patents

用于悬浮细胞的小规模养殖方法 Download PDF

Info

Publication number
CN108138128A
CN108138128A CN201680030077.0A CN201680030077A CN108138128A CN 108138128 A CN108138128 A CN 108138128A CN 201680030077 A CN201680030077 A CN 201680030077A CN 108138128 A CN108138128 A CN 108138128A
Authority
CN
China
Prior art keywords
culture
cells
scale
cell
small
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201680030077.0A
Other languages
English (en)
Chinese (zh)
Inventor
S·戈勒兹
R·施塔恩
S·克雷耶
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Glycotope GmbH
Original Assignee
Glycotope GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56108615&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN108138128(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Glycotope GmbH filed Critical Glycotope GmbH
Publication of CN108138128A publication Critical patent/CN108138128A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201680030077.0A 2015-05-29 2016-05-25 用于悬浮细胞的小规模养殖方法 Pending CN108138128A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
LU92727 2015-05-29
LU92727 2015-05-29
EP16151811 2016-01-19
EP16151811.3 2016-01-19
PCT/EP2016/061763 WO2016193083A1 (en) 2015-05-29 2016-05-25 Small scale cultivation method for suspension cells

Publications (1)

Publication Number Publication Date
CN108138128A true CN108138128A (zh) 2018-06-08

Family

ID=56108615

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680030077.0A Pending CN108138128A (zh) 2015-05-29 2016-05-25 用于悬浮细胞的小规模养殖方法

Country Status (10)

Country Link
US (1) US20180135091A1 (enExample)
EP (1) EP3303557B1 (enExample)
JP (1) JP6737813B2 (enExample)
KR (1) KR20180010200A (enExample)
CN (1) CN108138128A (enExample)
AU (1) AU2016272566A1 (enExample)
BR (1) BR112017023624A2 (enExample)
CA (1) CA2985435A1 (enExample)
RU (1) RU2730656C2 (enExample)
WO (1) WO2016193083A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111139216A (zh) * 2018-11-01 2020-05-12 上海药明生物技术有限公司 高通量、自动化的细胞连续培养方法及其应用
CN114599782A (zh) * 2019-07-01 2022-06-07 科学生物技术公司 用于在多并联生物反应器中培养粘附细胞的方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016272566A1 (en) 2015-05-29 2017-11-23 Glycotope Gmbh Small scale cultivation method for suspension cells
EP3472338B1 (en) 2016-06-20 2024-05-22 Octapharma AG Means and methods for modifying multiple alleles
EP3522128B1 (en) * 2016-09-29 2021-01-27 Coaido Inc. Disaster emergency system for simultaneously calling telephones in region
JP7317466B2 (ja) * 2017-12-12 2023-07-31 株式会社日立製作所 細胞株及び培養条件のスクリーニング方法、及びその装置
US20220177818A1 (en) * 2019-04-01 2022-06-09 The Automation Partnership (Cambridge ) Ltd. Operation process for a cell cultivation system
CN113795885A (zh) * 2019-05-08 2021-12-14 齐默尔根公司 缩小参数以设计在小规模下的微生物的实验及板模型以改进对在更大规模下的性能的预测
CN118064651B (zh) * 2024-03-11 2025-03-11 上海迈邦生物科技有限公司 一种细胞灌流培养体系维持稳态的方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0599651A2 (en) * 1992-11-25 1994-06-01 National Research Council Of Canada Bioreactor for the perfusion culture of cells
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
CN102690781A (zh) * 2012-04-28 2012-09-26 苏州金盟生物技术有限公司 一种灌流式细胞培养的培养基更换方法
US20130017577A1 (en) * 2009-11-17 2013-01-17 Medarex, Inc. Methods for enhanced protein production
CN103298925A (zh) * 2010-12-21 2013-09-11 葛莱高托普有限公司 用于培养人髓样白血病细胞以及由其衍生的细胞的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2997771B2 (ja) * 1998-06-29 2000-01-11 金沢大学長 細胞の懸濁培養方法
US20040185534A1 (en) * 2000-10-02 2004-09-23 Knudsen Ida Molgaard Industrial-scale serum-free production of recombinant proteins in mammalian cells
US20040185535A1 (en) 2003-03-21 2004-09-23 Giles Wilson Industrial-scale serum-free production of recombinant FVII in mammalian cells
WO2007071072A1 (en) 2005-12-22 2007-06-28 Corporation De L'ecole Polytechnique De Montreal High-rate perfusion bioreactor
EP2014760A1 (en) 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
EP2599651A1 (de) 2011-12-01 2013-06-05 Magna E-Car Systems GmbH & Co OG Heiz-/Kühlsystem für eine Batterie eines Kraftfahrzeugs sowie Betriebsverfahren hierfür
CN104195187B (zh) 2014-08-01 2018-02-16 润科生物工程（福建）有限公司 沉降‑逆灌流‑放流偶联制备富含dha油脂的方法及其专用细胞沉降槽
AU2016272566A1 (en) 2015-05-29 2017-11-23 Glycotope Gmbh Small scale cultivation method for suspension cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0599651A2 (en) * 1992-11-25 1994-06-01 National Research Council Of Canada Bioreactor for the perfusion culture of cells
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
US20130017577A1 (en) * 2009-11-17 2013-01-17 Medarex, Inc. Methods for enhanced protein production
CN103298925A (zh) * 2010-12-21 2013-09-11 葛莱高托普有限公司 用于培养人髓样白血病细胞以及由其衍生的细胞的方法
CN102690781A (zh) * 2012-04-28 2012-09-26 苏州金盟生物技术有限公司 一种灌流式细胞培养的培养基更换方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111139216A (zh) * 2018-11-01 2020-05-12 上海药明生物技术有限公司 高通量、自动化的细胞连续培养方法及其应用
CN114599782A (zh) * 2019-07-01 2022-06-07 科学生物技术公司 用于在多并联生物反应器中培养粘附细胞的方法

Also Published As

Publication number Publication date
RU2017140920A (ru) 2019-07-01
RU2017140920A3 (enExample) 2019-07-17
JP6737813B2 (ja) 2020-08-12
CA2985435A1 (en) 2016-12-08
EP3303557A1 (en) 2018-04-11
US20180135091A1 (en) 2018-05-17
AU2016272566A1 (en) 2017-11-23
RU2730656C2 (ru) 2020-08-24
EP3303557B1 (en) 2021-05-05
KR20180010200A (ko) 2018-01-30
JP2018516077A (ja) 2018-06-21
WO2016193083A1 (en) 2016-12-08
BR112017023624A2 (pt) 2018-07-17

Similar Documents

Publication Publication Date Title
CN108138128A (zh) 用于悬浮细胞的小规模养殖方法
Amanullah et al. Novel micro‐bioreactor high throughput technology for cell culture process development: Reproducibility and scalability assessment of fed‐batch CHO cultures
Yang et al. Perfusion seed cultures improve biopharmaceutical fed‐batch production capacity and product quality
Schwarz et al. Small-scale bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin
Huang et al. Maximizing productivity of CHO cell‐based fed‐batch culture using chemically defined media conditions and typical manufacturing equipment
Yang et al. Fed‐batch bioreactor process scale‐up from 3‐L to 2,500‐L scale for monoclonal antibody production from cell culture
Matanguihan et al. Upstream continuous processing: recent advances in production of biopharmaceuticals and challenges in manufacturing
US11390839B2 (en) Bioreactor system and method for producing a biopolymer
Kreye et al. A novel scale‐down mimic of perfusion cell culture using sedimentation in an automated microbioreactor (SAM)
KR20160145746A (ko) 발효 시스템
Qin et al. Open pond culture systems and photobioreactors for microalgal biofuel production
Wang et al. High‐throughput screening of antibody‐expressing CHO clones using an automated shaken deep‐well system
US9359427B2 (en) Methods for culturing human myeloid leukaemia cells and cells derived therefrom
JP2013544106A5 (enExample)
JP2025134848A (ja) 接種物を生産するためのプロセスおよびシステム
EP4008774A1 (en) Cell culture method and application thereof based on high-density and continuous inoculation
Dalm et al. Stable hybridoma cultivation in a pilot‐scale acoustic perfusion system: Long‐term process performance and effect of recirculation rate
CN110106139A (zh) 一种基于Tubespin生物反应器的缩小模型的细胞灌流培养方法
Alcântara et al. Evolution and design of continuous bioreactors for the production of biological products
CN113846017A (zh) 选择截留装置和降低截留率的方法
Hobisch et al. Automated feeding and fermentation phase transitions for the production of unspecific peroxygenase by Pichia pastoris
Ott et al. Perfusion-Based Antibody Production in the Ambr® 250 Modular
Neubauer et al. Media for Small-Scale Protein Production: Ready-to-Use Media Designed to Provide Controlled Cell Growth in Fed-Batch Mode
Wolf Development and optimization of mammalian cell perfusion cultures for continuous biomanufacturing
Oosterhuis et al. The CELL-tainer® single-use bioreactor, an opportunity for a high-performance bioprocess

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20180608

WD01 Invention patent application deemed withdrawn after publication