RU2721799C2 - Уменьшение содержания табак-специфичных нитрозаминов посредством изменения пути ассимиляции нитратов - Google Patents

Уменьшение содержания табак-специфичных нитрозаминов посредством изменения пути ассимиляции нитратов Download PDF

Info

Publication number
RU2721799C2
RU2721799C2 RU2017114356A RU2017114356A RU2721799C2 RU 2721799 C2 RU2721799 C2 RU 2721799C2 RU 2017114356 A RU2017114356 A RU 2017114356A RU 2017114356 A RU2017114356 A RU 2017114356A RU 2721799 C2 RU2721799 C2 RU 2721799C2
Authority
RU
Russia
Prior art keywords
tobacco
plant
nitrate reductase
plants
modified
Prior art date
Application number
RU2017114356A
Other languages
English (en)
Russian (ru)
Other versions
RU2017114356A3 (https=
RU2017114356A (ru
Inventor
Цзяньли ЛУ
Рэмси С. ЛЬЮИС
Ральф ДЬЮИ
Люсьен БОВЕ
Original Assignee
Филип Моррис Продактс С.А.
Норт Каролина Стейт Юниверсити
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Филип Моррис Продактс С.А., Норт Каролина Стейт Юниверсити filed Critical Филип Моррис Продактс С.А.
Publication of RU2017114356A publication Critical patent/RU2017114356A/ru
Publication of RU2017114356A3 publication Critical patent/RU2017114356A3/ru
Application granted granted Critical
Publication of RU2721799C2 publication Critical patent/RU2721799C2/ru

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A24B15/241—Extraction of specific substances
    • A24B15/245—Nitrosamines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/20—Biochemical treatment
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01001—Nitrate reductase (NADH) (1.7.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01002—Nitrate reductase [NAD(P)H] (1.7.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01003—Nitrate reductase (NADPH) (1.7.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
RU2017114356A 2014-09-26 2015-09-23 Уменьшение содержания табак-специфичных нитрозаминов посредством изменения пути ассимиляции нитратов RU2721799C2 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14186705.1 2014-09-26
EP14186705 2014-09-26
EP14194798 2014-11-25
EP14194798.6 2014-11-25
PCT/EP2015/071919 WO2016046288A1 (en) 2014-09-26 2015-09-23 Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway

Publications (3)

Publication Number Publication Date
RU2017114356A RU2017114356A (ru) 2018-10-29
RU2017114356A3 RU2017114356A3 (https=) 2019-04-29
RU2721799C2 true RU2721799C2 (ru) 2020-05-22

Family

ID=54148547

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2017114356A RU2721799C2 (ru) 2014-09-26 2015-09-23 Уменьшение содержания табак-специфичных нитрозаминов посредством изменения пути ассимиляции нитратов

Country Status (12)

Country Link
US (1) US11666082B2 (https=)
EP (1) EP3198015A1 (https=)
JP (1) JP6921740B2 (https=)
KR (1) KR102524798B1 (https=)
CN (1) CN106998787A (https=)
BR (1) BR112017003842B1 (https=)
CA (1) CA2959245A1 (https=)
CO (1) CO2017004076A2 (https=)
MX (1) MX2017003932A (https=)
PH (1) PH12017500313A1 (https=)
RU (1) RU2721799C2 (https=)
WO (1) WO2016046288A1 (https=)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8466187B2 (en) 2007-09-18 2013-06-18 Thermolife International, Llc Amino acid compositions
US10900034B2 (en) 2014-12-03 2021-01-26 Agilent Technologies, Inc. Guide RNA with chemical modifications
EP3280803B1 (en) 2015-04-06 2021-05-26 The Board of Trustees of the Leland Stanford Junior University Chemically modified guide rnas for crispr/cas-mediated gene regulation
WO2016179356A1 (en) * 2015-05-05 2016-11-10 North Carolina State University Methods and compositions for reducing the tobacco specific nitrosamine nnk in tobacco
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
CN106036984B (zh) * 2016-07-03 2017-08-08 赖高生 一种固定化蜗牛酶制备烟草浸膏的方法
AR115022A1 (es) 2018-03-28 2020-11-18 Philip Morris Products Sa Modulación del contenido de aminoácidos en una planta
AR115023A1 (es) 2018-03-28 2020-11-18 Philip Morris Products Sa Modulación del contenido de azúcar reductor en una planta
CN108424922B (zh) * 2018-04-20 2021-04-16 云南省烟草农业科学研究院 一种降低尼古丁转化率的cyp82e10基因错义突变体m271及其应用
GB201812603D0 (en) * 2018-08-02 2018-09-19 British American Tobacco Investments Ltd Method
DE102018216970A1 (de) * 2018-10-04 2020-04-09 Robert Bosch Gmbh Brennstoffeinspritzvorrichtung
US11976286B2 (en) * 2018-12-30 2024-05-07 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase
WO2022192743A1 (en) * 2021-03-11 2022-09-15 Thermolife International, Llc Enhanced nitrate, elemental metal, and amino acid compositions and methods of use
JP2022552638A (ja) 2019-10-01 2022-12-19 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物における糖およびアミノ酸含有量の調節（ｓｕｌｔｒ３）
JP7659547B2 (ja) 2019-10-01 2025-04-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物における還元糖含有量の調節（ｉｎｖ）
CN110747208B (zh) * 2019-11-28 2022-04-22 海南大学 一种木薯硝酸还原酶基因及其过量表达载体的构建和抗病应用
CN110819640B (zh) * 2019-12-19 2022-06-21 中国烟草总公司郑州烟草研究院 烟草NtNRP1基因其应用
CN115516098A (zh) * 2020-01-17 2022-12-23 奥驰亚客户服务有限公司 与改进的氮利用效率有关的方法和组合物
US11865139B2 (en) 2020-11-12 2024-01-09 Thermolife International, Llc Method of treating migraines and headaches
US12156886B2 (en) 2020-11-12 2024-12-03 Thermolife International, Llc Methods of increasing blood oxygen saturation
WO2022124361A1 (ja) * 2020-12-09 2022-06-16 日本たばこ産業株式会社 タバコ植物及びたばこ製品
MX2023009015A (es) 2021-02-11 2023-08-08 Thermolife Int Llc Un metodo para administrar gas de oxido nitrico.
JP2024534945A (ja) 2021-09-10 2024-09-26 アジレント・テクノロジーズ・インク 化学修飾を有するプライム編集のためのガイドｒｎａ
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
US20240132900A1 (en) * 2022-02-24 2024-04-25 University Of Florida Research Foundation, Incorporated T-DNA Free Gene Editing through Transient Suppressing POLQ in Plants
JP2025533986A (ja) 2022-10-13 2025-10-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム Ｎｔｐ２を調節することによって葉バイオマスおよび窒素利用効率を増加させる
JP2026506774A (ja) 2023-02-02 2026-02-26 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 糖トランスポーターの調節
EP4658670A1 (en) 2023-02-02 2025-12-10 Philip Morris Products S.A. Modulation of genes coding for lysine ketoglutarate reductase
CN121335615A (zh) 2023-06-29 2026-01-13 菲利普莫里斯生产公司 编码谷氨酸脱氢酶的基因的调节
WO2026057635A1 (en) 2024-09-12 2026-03-19 Philip Morris Products S.A. Modulation of nitrate levels in tobacco via mutation of nitrate reductase

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
WO2013140155A1 (en) * 2012-03-20 2013-09-26 British Americal Tobacco (Investments) Limited Transgenic plants with altered nitrate levels in leaves

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
WO1994002620A2 (en) 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
US6911541B2 (en) * 2000-08-30 2005-06-28 North Carolina State University Promoter fragment that is recognized by the product of the tobacco Nic gene
EA200400013A1 (ru) * 2001-06-08 2004-06-24 Вектор Тобакко Лтд. Модификация содержания никотина и нитрозамина в табаке
EA200600449A1 (ru) * 2003-08-19 2006-08-25 ТВЕНТИ СЕКОНД СЕНЧУРИ ЛИМИТИД, эЛэЛСи Табачное изделие (варианты), способ его изготовления (варианты), трансгенное растение табака, способы повышения содержания никотина, редуцирующих сахаров и уровня биосинтеза алкалоидов в растении и способ изготовления восстановленного табака
RU2257711C2 (ru) * 2003-10-06 2005-08-10 Всероссийский научно-исследовательский институт защиты растений Композиция на основе хитозана, снижающая содержание нитратов в растениеводческой продукции
CN1867674B (zh) * 2003-10-16 2011-11-09 美国无烟烟草有限责任公司 从烟草克隆细胞色素p450基因
US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
CA2666383C (en) * 2006-10-13 2015-12-22 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
CN101686731B (zh) * 2007-02-23 2013-05-08 美国无烟烟草有限责任公司 新型烟草组合物及制备方法
US8624084B2 (en) * 2007-03-16 2014-01-07 Gloria Coruzzi Pericycle-specific expression of microrna 167 in plants
US8124851B2 (en) * 2007-11-12 2012-02-28 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
US9247706B2 (en) 2010-01-15 2016-02-02 North Carolina State University Compositions and methods for minimizing nornicotine synthesis in tobacco
EP2537929B1 (en) * 2010-02-17 2017-04-12 Japan Tobacco, Inc. Plant component regulation factor, and use thereof
EP2619220A1 (en) * 2010-09-22 2013-07-31 British American Tobacco (Investments) Limited Transgenic plants
JP2014515596A (ja) 2011-02-28 2014-07-03 ノース カロライナ ステイト ユニヴァーシティ タバコ近交系植物ｎｃｂｅｘ１ｆ、ｎｃｂｅｘ１ｍｓ、及びｎｃｅｘ９０
ITMI20121419A1 (it) * 2012-08-08 2014-02-09 Fattoria Autonoma Tabacchi S C A R L Metodo per la produzione di tabacco volto a ridurre il tenore di nitrosammine.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
WO2013140155A1 (en) * 2012-03-20 2013-09-26 British Americal Tobacco (Investments) Limited Transgenic plants with altered nitrate levels in leaves

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GETU BEYENE et al., Two new cysteine proteinases with specific expression patterns in mature and senescent tobacco (Nicotiana tabacum L.) leaves, Journal of Experimental Botany, 2006, Vol. 57, No. 6, pp. 1431-1443. *
LAURENT NUSSAUME et al., Post-Transcriptional Regulation of Nitrate Reductase by Light Is Abolished by an N-Terminal Deletion, The Plant Cell, 1995, Vol. 7, pp.611-621 *
UNNI S. LEA et al., Posttranslational Regulation of Nitrate Reductase Strongly Affects the Levels of Free Amino Acids and Nitrate, whereas Transcriptional Regulation Has Only Minor Influence, Plant Physiol, 2006, Vol. 140, pp.1085-1094. *
UNNI S. LEA et al., Posttranslational Regulation of Nitrate Reductase Strongly Affects the Levels of Free Amino Acids and Nitrate, whereas Transcriptional Regulation Has Only Minor Influence, Plant Physiol, 2006, Vol. 140, pp.1085-1094. LAURENT NUSSAUME et al., Post-Transcriptional Regulation of Nitrate Reductase by Light Is Abolished by an N-Terminal Deletion, The Plant Cell, 1995, Vol. 7, pp.611-621. *

Also Published As

Publication number Publication date
PH12017500313A1 (en) 2017-07-17
KR20170078599A (ko) 2017-07-07
JP2017529850A (ja) 2017-10-12
MX2017003932A (es) 2017-06-30
KR102524798B1 (ko) 2023-04-24
US11666082B2 (en) 2023-06-06
CO2017004076A2 (es) 2017-09-20
RU2017114356A3 (https=) 2019-04-29
RU2017114356A (ru) 2018-10-29
CA2959245A1 (en) 2016-03-31
WO2016046288A1 (en) 2016-03-31
CN106998787A (zh) 2017-08-01
BR112017003842B1 (pt) 2024-02-20
BR112017003842A2 (pt) 2017-12-19
EP3198015A1 (en) 2017-08-02
JP6921740B2 (ja) 2021-08-18
US20170303582A1 (en) 2017-10-26

Similar Documents

Publication Publication Date Title
RU2721799C2 (ru) Уменьшение содержания табак-специфичных нитрозаминов посредством изменения пути ассимиляции нитратов
US20240376488A1 (en) Tobacco plants comprising reduced nicotine and reduced tobacco specific nitrosamines
JP7280245B2 (ja) タバコにおける改善された窒素利用効率に関する方法および組成物
JP6956084B2 (ja) タバコベルベリンブリッジ酵素様の核酸の標的化された突然変異誘発
KR20170002444A (ko) 식물 내 니코틴의 노르니코틴으로의 변환 감소
US20240218386A1 (en) Compositions and Methods for Producing Tobacco Plants and Products Having Altered Alkaloid Levels
KR20140057642A (ko) 니코티아나 타바쿰으로부터의 트레오닌 합성효소 및 그의 방법 및 용도
CN113271768A (zh) 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平
US20250243501A1 (en) Compositions and methods for producing tobacco plants and products having altered alkaloid levels with desirable leaf quality via manipulating leaf quality genes
WO2021247740A1 (en) Compositions and methods for producing tobacco plants and products having altered alkaloid levels
JP2026035667A (ja) タバコにおける淡黄色遺伝子座およびその応用
US20190309317A1 (en) Compositions and methods for producing tobacco plants and products having altered alkaloid levels
WO2023230433A1 (en) Methods and compositions for regulating alkaloids in tobacco field
WO2024031038A1 (en) Methods and compositions for regulating alkaloids in tobacco
US20210230627A1 (en) Methods and compositions related to improved nitrogen use efficiency