RU2696071C2 - Получение плюрипотентных клеток de novo - Google Patents
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- RU2696071C2 RU2696071C2 RU2014147093A RU2014147093A RU2696071C2 RU 2696071 C2 RU2696071 C2 RU 2696071C2 RU 2014147093 A RU2014147093 A RU 2014147093A RU 2014147093 A RU2014147093 A RU 2014147093A RU 2696071 C2 RU2696071 C2 RU 2696071C2
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| WO2015117021A1 (en) | 2014-01-31 | 2015-08-06 | Factor Bioscience Inc. | Methods and products for nucleic acid production and delivery |
| RU2711942C2 (ru) * | 2014-03-19 | 2020-01-23 | Виселл Терапеутикс, Инк. | Способы, относящиеся к плюрипотентным клеткам |
| JP6986968B2 (ja) * | 2014-12-04 | 2021-12-22 | ステモン・インコーポレイテッド | エネルギーを利用した多能性細胞誘導装置及び方法 |
| WO2016089178A1 (ko) * | 2014-12-04 | 2016-06-09 | 가톨릭관동대학교산학협력단 | 에너지를 이용한 다능성 세포 유도 장치 및 방법 |
| EP3543339A1 (en) | 2015-02-13 | 2019-09-25 | Factor Bioscience Inc. | Nucleic acid products and methods of administration thereof |
| WO2016203477A1 (en) * | 2015-06-18 | 2016-12-22 | Yeda Research And Development Co. Ltd. | Conditioning protocols and use of same for tissue regeneration |
| KR102050852B1 (ko) * | 2016-03-11 | 2019-12-03 | 주식회사 스템온 | 세포 리프로그래밍 장치 |
| KR101855967B1 (ko) * | 2016-03-11 | 2018-05-10 | 가톨릭관동대학교산학협력단 | 물리적 자극에 의한 환경유입을 이용한 세포 리프로그래밍 방법 |
| WO2017155167A1 (ko) * | 2016-03-11 | 2017-09-14 | 가톨릭관동대학교산학협력단 | 세포 리프로그래밍 장치 |
| WO2017155166A1 (ko) * | 2016-03-11 | 2017-09-14 | 가톨릭관동대학교기술지주 주식회사 | 물리적 자극에 의한 환경유입을 이용한 세포 리프로그래밍 방법 |
| US10456423B2 (en) | 2016-06-13 | 2019-10-29 | SMART SURGICAL, Inc. | Compositions for biological systems and methods for preparing and using the same |
| US10426796B2 (en) | 2016-06-13 | 2019-10-01 | SMART SURGICAL, Inc. | Compositions for biological systems and methods for preparing and using the same |
| CN109803977B (zh) | 2016-08-17 | 2023-03-17 | 菲克特生物科学股份有限公司 | 核酸产品及其施用方法 |
| US20180127738A1 (en) * | 2016-11-07 | 2018-05-10 | BiomediStem, LLC | Production and therapeutic uses of epinul pluripotent cells and differentiated cells derived therefrom |
| EP3601530A1 (en) * | 2017-03-20 | 2020-02-05 | IFOM Fondazione Istituto Firc di Oncologia Molecolare | Method of generating 2 cell-like stem cells |
| WO2018226435A1 (en) * | 2017-06-10 | 2018-12-13 | SMART SURGICAL, Inc. | Compositions for biological systems and methods for preparing and using the same |
| CN107729932B (zh) * | 2017-10-10 | 2019-07-26 | 杭州智微信息科技有限公司 | 骨髓细胞标记方法和系统 |
| KR102416171B1 (ko) | 2018-10-02 | 2022-07-06 | 주식회사 스템온 | 세포의 리프로그래밍을 유도하는 리프로좀 및 그 제조 방법 |
| WO2021003462A1 (en) | 2019-07-03 | 2021-01-07 | Factor Bioscience Inc. | Cationic lipids and uses thereof |
| US10501404B1 (en) | 2019-07-30 | 2019-12-10 | Factor Bioscience Inc. | Cationic lipids and transfection methods |
| JP2022546578A (ja) * | 2019-09-04 | 2022-11-04 | ブイセル セラピューティックス,インコーポレイテッド | 癌を処置するためのワクチンおよびストレスリプログラミングによるその作製方法 |
| AU2021218762A1 (en) | 2020-02-14 | 2022-10-06 | Hackensack Meridian Health, Inc. | Platelet-derived mitochondria treatment and method of generating multipotent cells |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2215539C2 (ru) * | 1995-02-02 | 2003-11-10 | ДХУТ Гхази Джасвиндер | Способ получения недифференцированной клетки |
| US20070190646A1 (en) * | 2006-02-10 | 2007-08-16 | The Trustees Of The University Of Pennsylvania | Regulating stem cell differentiation by controlling matrix elasticity |
| WO2008038148A2 (en) * | 2006-05-11 | 2008-04-03 | Andrew Craig Boquest | Stem cells and methods of making and using stem cells |
| US20110070647A1 (en) * | 2009-07-15 | 2011-03-24 | Mari Dezawa | Pluripotent stem cell that can be isolated from body tissue |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4963489A (en) | 1987-04-14 | 1990-10-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| FR2687404B1 (fr) | 1992-02-19 | 1994-05-20 | Centre Nal Recherc Scientifique | Peptides immunologiquement apparentes aux proteines d'un agent viral et leurs applications biologiques. |
| AU4543193A (en) | 1992-06-22 | 1994-01-24 | Henry E. Young | Scar inhibitory factor and use thereof |
| US5672346A (en) | 1992-07-27 | 1997-09-30 | Indiana University Foundation | Human stem cell compositions and methods |
| CA2146747C (en) | 1992-10-09 | 2006-12-19 | Brian A. Naughton | Liver reserve cells |
| US5753431A (en) * | 1993-10-13 | 1998-05-19 | Northeastern Ohio University | Cholesterol 7 α-hydroxdylase gene regulatory elements and transcription factors |
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| WO2002057428A1 (en) | 2000-10-30 | 2002-07-25 | University Of Massachusetts | Isolation of spore-like cells from tissues exposed to extreme conditions |
| GB0113118D0 (en) * | 2001-05-31 | 2001-07-18 | Intercytex Ltd | Stem Cells |
| US7575321B2 (en) | 2003-10-30 | 2009-08-18 | Welch Allyn, Inc. | Apparatus and method of diagnosis of optically identifiable ophthalmic conditions |
| JP4676442B2 (ja) * | 2003-12-02 | 2011-04-27 | セラヴィー バイオサイエンシズ エルエルシー | 神経前駆細胞を増殖させるための組成物および方法 |
| EP1789540B9 (en) * | 2004-09-03 | 2012-02-22 | Moraga Biotechnology Inc. | Non-embryonic totipotent blastomer-like stem cells and methods therefor |
| US20060084167A1 (en) | 2004-10-16 | 2006-04-20 | Cohenford Menashi A | Formation of Hybrid Cells by Fusion of Lineage Committed Cells with Stem Cells |
| US20090298095A1 (en) * | 2005-04-15 | 2009-12-03 | The John Hopkins University | Immortalization of cells including neuronal cells |
| FR2885368A1 (fr) * | 2005-05-04 | 2006-11-10 | Fred Zacouto | Systemes et procedes de regeneration cellulaire et utilisations de telles cellules regenerees |
| CA2621161A1 (en) * | 2005-09-08 | 2007-03-15 | The Government Of The United States Of America As Represented By The Sec Retary Of The Department Of Health And Human Services | Methods for promoting stem cell proliferation and survival |
| US20070087437A1 (en) * | 2005-10-14 | 2007-04-19 | Jifan Hu | Methods for rejuvenating cells in vitro and in vivo |
| US8278104B2 (en) * | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
| US20100135970A1 (en) * | 2006-10-27 | 2010-06-03 | Caritas St. Elizabeth Medical Center Of Boston, In | Methods for Reprogramming Adult Somatic Cells and Uses Thereof |
| CN105861443A (zh) * | 2007-04-07 | 2016-08-17 | 怀特黑德生物医学研究所 | 体细胞重编程 |
| CA2704220A1 (en) * | 2007-10-30 | 2009-05-07 | Le Centre Nationale De La Recherche Scientifique | Method for conducting an assay for neutralizing antibodies |
| KR101564044B1 (ko) | 2007-10-31 | 2015-10-28 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 핵초기화 방법 |
| US20090280518A1 (en) * | 2008-05-12 | 2009-11-12 | Massachusetts Institute Of Technology | System for high throughput measurement of mechanical properties of cells |
| EP2492715B1 (en) * | 2009-10-06 | 2019-01-09 | Canon Kabushiki Kaisha | Rear attachment lens, imaging optical system, and image pickup apparatus |
| JPWO2011125948A1 (ja) * | 2010-04-02 | 2013-07-11 | 独立行政法人理化学研究所 | Es細胞の製造方法 |
| KR20120002134A (ko) | 2010-06-30 | 2012-01-05 | 서울대학교산학협력단 | 지방 기질 세포의 역분화를 유도하는 방법 |
| ES3005334T3 (en) | 2010-07-09 | 2025-03-14 | Gid Bio Inc | Apparatus and methods relating to collecting and processing human biological material containing adipose |
-
2013
- 2013-04-24 WO PCT/US2013/037996 patent/WO2013163296A1/en not_active Ceased
- 2013-04-24 EP EP18203532.9A patent/EP3483261B1/en active Active
- 2013-04-24 ES ES18203532T patent/ES2972525T3/es active Active
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- 2013-04-24 US US14/397,080 patent/US11963977B2/en active Active
- 2013-04-24 KR KR1020147032963A patent/KR20150045935A/ko not_active Ceased
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- 2013-04-24 CN CN201380033613.9A patent/CN104718283A/zh active Pending
- 2013-04-24 AU AU2013251649A patent/AU2013251649B2/en active Active
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-
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- 2018-06-20 JP JP2018117481A patent/JP2018183145A/ja not_active Withdrawn
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- 2019-06-05 AU AU2019203943A patent/AU2019203943B2/en active Active
-
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- 2020-07-16 JP JP2020122035A patent/JP2020182480A/ja not_active Withdrawn
-
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- 2022-11-01 JP JP2022175459A patent/JP2023002805A/ja active Pending
-
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- 2025-04-03 JP JP2025061922A patent/JP2025106394A/ja active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2215539C2 (ru) * | 1995-02-02 | 2003-11-10 | ДХУТ Гхази Джасвиндер | Способ получения недифференцированной клетки |
| US20070190646A1 (en) * | 2006-02-10 | 2007-08-16 | The Trustees Of The University Of Pennsylvania | Regulating stem cell differentiation by controlling matrix elasticity |
| WO2008038148A2 (en) * | 2006-05-11 | 2008-04-03 | Andrew Craig Boquest | Stem cells and methods of making and using stem cells |
| US20110070647A1 (en) * | 2009-07-15 | 2011-03-24 | Mari Dezawa | Pluripotent stem cell that can be isolated from body tissue |
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