RU2619784C2 - Система и набор для получения цитологических образцов для исследования - Google Patents
Система и набор для получения цитологических образцов для исследования Download PDFInfo
- Publication number
- RU2619784C2 RU2619784C2 RU2014114524A RU2014114524A RU2619784C2 RU 2619784 C2 RU2619784 C2 RU 2619784C2 RU 2014114524 A RU2014114524 A RU 2014114524A RU 2014114524 A RU2014114524 A RU 2014114524A RU 2619784 C2 RU2619784 C2 RU 2619784C2
- Authority
- RU
- Russia
- Prior art keywords
- sample
- dye
- cells
- cell
- cytological
- Prior art date
Links
- 230000002380 cytological effect Effects 0.000 title claims abstract description 38
- 238000011160 research Methods 0.000 title claims description 6
- 210000004027 cell Anatomy 0.000 claims abstract description 183
- 238000000034 method Methods 0.000 claims abstract description 63
- 238000002360 preparation method Methods 0.000 claims abstract description 59
- 230000001086 cytosolic effect Effects 0.000 claims abstract description 28
- 238000000576 coating method Methods 0.000 claims abstract description 21
- 239000011248 coating agent Substances 0.000 claims abstract description 19
- 239000003607 modifier Substances 0.000 claims abstract description 16
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 11
- 201000011510 cancer Diseases 0.000 claims abstract description 10
- 238000012216 screening Methods 0.000 claims abstract description 5
- 238000003745 diagnosis Methods 0.000 claims abstract description 3
- 238000011275 oncology therapy Methods 0.000 claims abstract description 3
- 238000004393 prognosis Methods 0.000 claims abstract description 3
- 238000002560 therapeutic procedure Methods 0.000 claims abstract description 3
- 239000000523 sample Substances 0.000 claims description 153
- 239000000203 mixture Substances 0.000 claims description 66
- 238000010186 staining Methods 0.000 claims description 38
- 239000000834 fixative Substances 0.000 claims description 34
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 25
- 239000003795 chemical substances by application Substances 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 23
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 18
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims description 16
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 16
- 239000006185 dispersion Substances 0.000 claims description 13
- 239000000243 solution Substances 0.000 claims description 12
- 238000004458 analytical method Methods 0.000 claims description 11
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 claims description 10
- 229960000583 acetic acid Drugs 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 10
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 239000012472 biological sample Substances 0.000 claims description 8
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 8
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 claims description 8
- 239000002953 phosphate buffered saline Substances 0.000 claims description 8
- 230000007935 neutral effect Effects 0.000 claims description 7
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 6
- 239000003146 anticoagulant agent Substances 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 210000003679 cervix uteri Anatomy 0.000 claims description 5
- 239000012362 glacial acetic acid Substances 0.000 claims description 5
- 230000000007 visual effect Effects 0.000 claims description 5
- 229940127219 anticoagulant drug Drugs 0.000 claims description 4
- 239000002738 chelating agent Substances 0.000 claims description 4
- 210000003097 mucus Anatomy 0.000 claims description 4
- CDULGHZNHURECF-UHFFFAOYSA-N 2,3-dimethylaniline 2,4-dimethylaniline 2,5-dimethylaniline 2,6-dimethylaniline 3,4-dimethylaniline 3,5-dimethylaniline Chemical group CC1=CC=C(N)C(C)=C1.CC1=CC=C(C)C(N)=C1.CC1=CC(C)=CC(N)=C1.CC1=CC=C(N)C=C1C.CC1=CC=CC(N)=C1C.CC1=CC=CC(C)=C1N CDULGHZNHURECF-UHFFFAOYSA-N 0.000 claims description 3
- 239000000090 biomarker Substances 0.000 claims description 2
- 235000012730 carminic acid Nutrition 0.000 claims description 2
- 238000002573 colposcopy Methods 0.000 claims description 2
- XJCPMUIIBDVFDM-UHFFFAOYSA-M nile blue A Chemical compound [Cl-].C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4[O+]=C3C=C(N)C2=C1 XJCPMUIIBDVFDM-UHFFFAOYSA-M 0.000 claims description 2
- OARRHUQTFTUEOS-UHFFFAOYSA-N safranin Chemical compound [Cl-].C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 OARRHUQTFTUEOS-UHFFFAOYSA-N 0.000 claims description 2
- UJMBCXLDXJUMFB-GLCFPVLVSA-K tartrazine Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)C1=NN(C=2C=CC(=CC=2)S([O-])(=O)=O)C(=O)C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 UJMBCXLDXJUMFB-GLCFPVLVSA-K 0.000 claims description 2
- 235000012756 tartrazine Nutrition 0.000 claims description 2
- 229960000943 tartrazine Drugs 0.000 claims description 2
- 239000004149 tartrazine Substances 0.000 claims description 2
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 16
- 230000004048 modification Effects 0.000 abstract description 7
- 238000012986 modification Methods 0.000 abstract description 7
- 239000003086 colorant Substances 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000000975 dye Substances 0.000 description 98
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 27
- HSXUHWZMNJHFRV-QIKYXUGXSA-L orange G Chemical compound [Na+].[Na+].OC1=CC=C2C=C(S([O-])(=O)=O)C=C(S([O-])(=O)=O)C2=C1\N=N\C1=CC=CC=C1 HSXUHWZMNJHFRV-QIKYXUGXSA-L 0.000 description 17
- 210000000805 cytoplasm Anatomy 0.000 description 13
- 150000001875 compounds Chemical class 0.000 description 12
- ZHAFUINZIZIXFC-UHFFFAOYSA-N [9-(dimethylamino)-10-methylbenzo[a]phenoxazin-5-ylidene]azanium;chloride Chemical compound [Cl-].O1C2=CC(=[NH2+])C3=CC=CC=C3C2=NC2=C1C=C(N(C)C)C(C)=C2 ZHAFUINZIZIXFC-UHFFFAOYSA-N 0.000 description 11
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 11
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 11
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 11
- 206010008342 Cervix carcinoma Diseases 0.000 description 9
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 9
- 201000010881 cervical cancer Diseases 0.000 description 9
- 239000002253 acid Substances 0.000 description 8
- 239000004094 surface-active agent Substances 0.000 description 8
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 7
- 210000004940 nucleus Anatomy 0.000 description 7
- 238000009595 pap smear Methods 0.000 description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 241000701806 Human papillomavirus Species 0.000 description 4
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 210000003855 cell nucleus Anatomy 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000010191 image analysis Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 239000001509 sodium citrate Substances 0.000 description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 229910019093 NaOCl Inorganic materials 0.000 description 3
- 239000013504 Triton X-100 Substances 0.000 description 3
- 229920004890 Triton X-100 Polymers 0.000 description 3
- RZUBARUFLYGOGC-MTHOTQAESA-L acid fuchsin Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=C(N)C(C)=CC(C(=C\2C=C(C(=[NH2+])C=C/2)S([O-])(=O)=O)\C=2C=C(C(N)=CC=2)S([O-])(=O)=O)=C1 RZUBARUFLYGOGC-MTHOTQAESA-L 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000012303 cytoplasmic staining Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- SEACYXSIPDVVMV-UHFFFAOYSA-L eosin Y Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(Br)C=C21 SEACYXSIPDVVMV-UHFFFAOYSA-L 0.000 description 3
- 230000009969 flowable effect Effects 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 3
- 238000012758 nuclear staining Methods 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 125000006850 spacer group Chemical group 0.000 description 3
- MAGFQRLKWCCTQJ-UHFFFAOYSA-M 4-ethenylbenzenesulfonate Chemical compound [O-]S(=O)(=O)C1=CC=C(C=C)C=C1 MAGFQRLKWCCTQJ-UHFFFAOYSA-M 0.000 description 2
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 2
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 2
- 229920002845 Poly(methacrylic acid) Polymers 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 2
- DGOBMKYRQHEFGQ-UHFFFAOYSA-L acid green 5 Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 DGOBMKYRQHEFGQ-UHFFFAOYSA-L 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000015271 coagulation Effects 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000011928 denatured alcohol Substances 0.000 description 2
- 229960000633 dextran sulfate Drugs 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 229940051132 light green sf yellowish Drugs 0.000 description 2
- 210000004379 membrane Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000012046 mixed solvent Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 210000004400 mucous membrane Anatomy 0.000 description 2
- 150000003901 oxalic acid esters Chemical class 0.000 description 2
- 230000002085 persistent effect Effects 0.000 description 2
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000011120 smear test Methods 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 description 2
- 230000008719 thickening Effects 0.000 description 2
- XOSXWYQMOYSSKB-LDKJGXKFSA-L water blue Chemical compound CC1=CC(/C(\C(C=C2)=CC=C2NC(C=C2)=CC=C2S([O-])(=O)=O)=C(\C=C2)/C=C/C\2=N\C(C=C2)=CC=C2S([O-])(=O)=O)=CC(S(O)(=O)=O)=C1N.[Na+].[Na+] XOSXWYQMOYSSKB-LDKJGXKFSA-L 0.000 description 2
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- LOMVENUNSWAXEN-UHFFFAOYSA-N Methyl oxalate Chemical compound COC(=O)C(=O)OC LOMVENUNSWAXEN-UHFFFAOYSA-N 0.000 description 1
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 1
- HSNVNALJRSJDHT-UHFFFAOYSA-N P(=O)(=O)[Mo] Chemical compound P(=O)(=O)[Mo] HSNVNALJRSJDHT-UHFFFAOYSA-N 0.000 description 1
- 208000006994 Precancerous Conditions Diseases 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- JHIDGGPPGFZMES-UHFFFAOYSA-N acetic acid;n-(2-aminoethyl)hydroxylamine Chemical compound CC(O)=O.CC(O)=O.CC(O)=O.NCCNO JHIDGGPPGFZMES-UHFFFAOYSA-N 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 150000007514 bases Chemical class 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 150000001860 citric acid derivatives Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- -1 crimson crimson Chemical compound 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000011363 dried mixture Substances 0.000 description 1
- 210000004696 endometrium Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- SQHOAFZGYFNDQX-UHFFFAOYSA-N ethyl-[7-(ethylamino)-2,8-dimethylphenothiazin-3-ylidene]azanium;chloride Chemical compound [Cl-].S1C2=CC(=[NH+]CC)C(C)=CC2=NC2=C1C=C(NCC)C(C)=C2 SQHOAFZGYFNDQX-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229920006158 high molecular weight polymer Polymers 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 230000005741 malignant process Effects 0.000 description 1
- VHGXRGXCDVQIKS-KRWDZBQOSA-N methyl (2s)-3-(4-methylphenyl)sulfonyloxy-2-(phenylmethoxycarbonylamino)propanoate Chemical compound C([C@@H](C(=O)OC)NC(=O)OCC=1C=CC=CC=1)OS(=O)(=O)C1=CC=C(C)C=C1 VHGXRGXCDVQIKS-KRWDZBQOSA-N 0.000 description 1
- RFKJHQXSLBUONF-UHFFFAOYSA-N methyl blue free acid Chemical compound C1=CC(S(=O)(=O)O)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=NC=2C=CC(=CC=2)S(O)(=O)=O)C=2C=CC(NC=3C=CC(=CC=3)S(O)(=O)=O)=CC=2)C=C1 RFKJHQXSLBUONF-UHFFFAOYSA-N 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000003319 supportive effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161534045P | 2011-09-13 | 2011-09-13 | |
| US61/534,045 | 2011-09-13 | ||
| PCT/IB2012/054606 WO2013038306A1 (en) | 2011-09-13 | 2012-09-06 | System and kit for preparing a cytological sample for examination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2014114524A RU2014114524A (ru) | 2015-10-20 |
| RU2619784C2 true RU2619784C2 (ru) | 2017-05-18 |
Family
ID=47018337
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2014114524A RU2619784C2 (ru) | 2011-09-13 | 2012-09-06 | Система и набор для получения цитологических образцов для исследования |
Country Status (4)
| Country | Link |
|---|---|
| CN (1) | CN103827654B (zh) |
| MX (1) | MX2014002844A (zh) |
| RU (1) | RU2619784C2 (zh) |
| WO (1) | WO2013038306A1 (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU178938U1 (ru) * | 2017-06-20 | 2018-04-23 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Амурская государственная медицинская академия" Министерства здравоохранения Российской Федерации | Устройство для подготовки цитологического мазка биологических жидкостей к проведению экспресс-анализа клеточного состава |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10126216B2 (en) | 2011-02-17 | 2018-11-13 | Ventana Medical Systems, Inc. | Method for tissue sample fixation |
| WO2016041890A2 (en) * | 2014-09-17 | 2016-03-24 | Ventana Medical Systems, Inc. | Compositions, methods, and systems for tissue fixation |
| CN104390832A (zh) * | 2014-11-07 | 2015-03-04 | 刘志军 | 一种适用于血细胞的快速瑞氏染液的配方及制作方法 |
| JP6703599B2 (ja) * | 2015-09-14 | 2020-06-03 | エッセンリックス コーポレーション | 試料、特に血液を分析するための装置及びシステム、並びにそれらの使用方法 |
| CN108287240A (zh) * | 2018-01-29 | 2018-07-17 | 青岛浩铂生物科技有限公司 | 基于尿液中宫颈脱落细胞染色的检测宫颈癌试剂及制备方法 |
| CN108303416A (zh) * | 2018-01-29 | 2018-07-20 | 青岛浩铂生物科技有限公司 | 一种上皮组织染色液试剂盒及其制备方法 |
| CN112574938B (zh) * | 2019-09-29 | 2023-02-03 | 体必康生物科技(广东)股份有限公司 | 一种用于膜过滤富集细菌的痰处理液及其应用 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1555507A (en) * | 1975-08-08 | 1979-11-14 | Gen Electric | Slides |
| RU2089082C1 (ru) * | 1991-10-10 | 1997-09-10 | Сэмсонайт Корпорейшн | Кожгалантерейное изделие личного пользования и шарнир для такого изделия (варианты) |
| RU2184966C1 (ru) * | 2000-11-30 | 2002-07-10 | Боев Сергей Федотович | Способ анализа клеточного состава крови по мазку |
| US20030211452A1 (en) * | 2002-05-10 | 2003-11-13 | Vladimir Vincek | Preservation of RNA and morphology in cells and tissues |
| CN101598731A (zh) * | 2009-05-07 | 2009-12-09 | 陈志南 | 一种用于肿瘤病理诊断用途的免疫组织化学诊断试剂盒 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3796594A (en) * | 1970-10-30 | 1974-03-12 | Gen Electric | Stain coated slides for differentially staining blood |
| US3834874A (en) * | 1972-10-18 | 1974-09-10 | Gen Electric | Detection of malarial parasites in blood |
| DE2515869C3 (de) * | 1975-04-11 | 1979-11-08 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Vorrichtung zur Herstellung von vorbeschichteten Objektträgern |
| FR2320544A1 (fr) * | 1975-08-08 | 1977-03-04 | Gen Electric | Formulation et procede de coloration et de scellement d'un echantillon biologique |
| JPS60159630A (ja) * | 1984-01-31 | 1985-08-21 | Hisashi Tokita | 抗癌剤感受性試験のキツト |
| US5002736A (en) * | 1987-03-31 | 1991-03-26 | Fisher Scientific Co. | Microscope slide and slide assembly |
| GB9518129D0 (en) * | 1995-09-06 | 1995-11-08 | Zynocyte Ltd | Stain and capillary slide |
| ATE268470T1 (de) * | 1998-06-30 | 2004-06-15 | Lamina Inc | Zytologische und histologische fixier- zusammensetzung und verfahren zur verwendung |
| CN101852697B (zh) * | 2010-05-26 | 2012-05-02 | 福州泰普生物科学有限公司 | 一种检测脱落细胞的靶向染色试剂盒及其使用方法 |
-
2012
- 2012-09-06 MX MX2014002844A patent/MX2014002844A/es unknown
- 2012-09-06 WO PCT/IB2012/054606 patent/WO2013038306A1/en active Application Filing
- 2012-09-06 CN CN201280044631.2A patent/CN103827654B/zh not_active Expired - Fee Related
- 2012-09-06 RU RU2014114524A patent/RU2619784C2/ru not_active IP Right Cessation
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1555507A (en) * | 1975-08-08 | 1979-11-14 | Gen Electric | Slides |
| RU2089082C1 (ru) * | 1991-10-10 | 1997-09-10 | Сэмсонайт Корпорейшн | Кожгалантерейное изделие личного пользования и шарнир для такого изделия (варианты) |
| RU2184966C1 (ru) * | 2000-11-30 | 2002-07-10 | Боев Сергей Федотович | Способ анализа клеточного состава крови по мазку |
| US20030211452A1 (en) * | 2002-05-10 | 2003-11-13 | Vladimir Vincek | Preservation of RNA and morphology in cells and tissues |
| CN101598731A (zh) * | 2009-05-07 | 2009-12-09 | 陈志南 | 一种用于肿瘤病理诊断用途的免疫组织化学诊断试剂盒 |
Non-Patent Citations (1)
| Title |
|---|
| Селиванов В.Е. Справочник "Красители в биологии и медицине", И-"АЗБУКА", Барнаул 2003, с. 5-8. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU178938U1 (ru) * | 2017-06-20 | 2018-04-23 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Амурская государственная медицинская академия" Министерства здравоохранения Российской Федерации | Устройство для подготовки цитологического мазка биологических жидкостей к проведению экспресс-анализа клеточного состава |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103827654A (zh) | 2014-05-28 |
| WO2013038306A1 (en) | 2013-03-21 |
| MX2014002844A (es) | 2014-07-09 |
| RU2014114524A (ru) | 2015-10-20 |
| CN103827654B (zh) | 2017-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2619784C2 (ru) | Система и набор для получения цитологических образцов для исследования | |
| AU740441B2 (en) | Cytological and histological fixative composition and methods of use | |
| JP7454290B2 (ja) | 食道扁平上皮癌の初代細胞用の培養培地、及びその培養方法 | |
| US20060088814A1 (en) | Enhanced cell preservative solution and methods for using same | |
| KR100476428B1 (ko) | 액체 표본으로부터 미립자 물질을 분리하는 방법 및 장치 | |
| WO2006047252A1 (en) | Enhanced cell preseravtive solution and methods for using same | |
| RU2536502C2 (ru) | Цитологическая и гистологическая фиксирующая композиция и способ окрашивания | |
| CN112213172A (zh) | 一种稳定的阴道分泌物有形成分染色液及其制备方法 | |
| EP3199638B1 (en) | Cancer cell detection method using living body derived cells | |
| WO2005035736A1 (ja) | 粘液除去方法並びにこれに用いる細胞処理液及び保存液 | |
| JP2000187033A (ja) | 細胞染色液 | |
| Anchana et al. | Cytomorphologic analysis of wet fixed and air dried oral buccal smears rehydrated with normal saline: A comparative study | |
| JP6671681B2 (ja) | 検鏡標本の作製方法 | |
| TW202111305A (zh) | 生物材料染色組成物及方法 | |
| Rehfeld et al. | Histological methods | |
| RU2702237C1 (ru) | Способ приготовления препарата отделяемого слизистой влагалища для оценки микробиоценоза | |
| JP6974980B2 (ja) | 細胞処理試薬及び顕微鏡標本を作製する方法 | |
| DK2449377T3 (en) | Method for Detecting Tumor Cells by Fluorescence Signals | |
| US20230384199A1 (en) | Quality control substance used in urine sediment analyzer | |
| Liana et al. | The Technique of Rapid on-Site Evaluation on Lung Cancer | |
| WO2023174977A1 (en) | Sample preparation method for microscopic examination using a chitosan-based porous material | |
| JP2023177266A (ja) | 尿沈渣分析装置で使用される精度管理物質 | |
| UA132804U (uk) | Спосіб фарбування ооцист cryptosporidium spp. для високодиференційної люмінесцентно-мікроскопічної детекції |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20200907 |