RU2258501C2 - Method for applying ethidium bromide for treatment of horses with pyroplasmosis - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: method involves administration of ethidium bromide in the single dose 0.5-1.0 mg/kg of mass by intramuscular route. Invention promotes to effective treatment of horses with pyroplasmosis in combination with reducing toxic effect of ethidium bromide on the horse body. Invention can be used in treatment of horses with pyroplasmosis.

EFFECT: enhanced effectiveness of treatment.

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Description

The invention relates to veterinary medicine, and in particular to a method for the treatment of equine piroplasmosis with ethidium bromide.

Known drug azidine (berenyl) and its use as a pyroplasmacide. The drug is administered to animals twice, with an interval of 24 hours, intramuscularly at a dose of 3.5 mg per kg (see Abuladze K.I. et al. Parasitology and invasive diseases of farm animals. - M .: Agropromizdat, 1992. - P.291- 294).

Having high efficiency, this drug has several disadvantages. The administration of azidine to a weakened, depleted animal can cause intoxication, expressed in severe anxiety, diarrhea, after which a depressive state occurs, which reduces the effectiveness of treatment and lengthens the healing time of the animal. Due to the use of the drug for a long time, the formation of resistant strains of pyroplasm to this substance is not ruled out.

The closest solution adopted for the prototype is a well-known drug for the treatment of pyroplasmidoses of animals, ethidium bromide, which is administered twice with an interval of 24 hours at a dose of 0.5-1.0 mg per kg m. In addition, the animals are given ethidium bromide and azidine at a dose of 3.5 mg per kg g.m. as a rehabilitation pair (RU 2119792 dated 10/10/1998).

The disadvantage of this method is its significant toxicity and labor.

The technical problem solved by the invention is to reduce the toxic effects of ethidium bromide and reduce the frequency of its introduction.

The essence of the invention lies in the fact that in the known method of treating pyroplasmidoses in animals, comprising intramuscular administration of ethidium bromide at a dose of 0.5-1.0 mg / kg body weight, according to the invention, ethidium bromide is administered once to horses.

Example 1. For treatment, a 1% aqueous solution of ethidium bromide was used. The solution was prepared immediately before its introduction.

Six horses with pyroplasmosis were injected with a 1% aqueous solution of ethidium bromide, deeply intramuscularly at a dose of 0.5-1.0 mg / kg of live weight once. After administration, the general condition of the animals was monitored. Signs characteristic of intoxication were not noted. On the next day after administration of the drug, horses showed a decrease in temperature to normal, an improvement in appetite, and an increase in activity. Two days later, the absence of hemoglobinuria was established. 15 days after administration of ethidium bromide in all animals, clinical signs of pyroplasmosis were absent. Microscopy of blood smears did not reveal the causative agent of pyroplasmosis (P. caballi). During a clinical examination, deviations in the physiological state of the animals were not observed.

Example 2. To determine the comparative effectiveness of ethidium bromide introduced in the form of a 1% aqueous solution at a dose of 0.5-1.0 mg / kg body weight, one and two times with an interval of 24 hours formed two groups of animals experimental and control 8 patients with horse pyroplasmosis in each group. The diagnosis of pyroplasmosis was made taking into account the clinical manifestations of the disease and the results of the study of blood smears. The solution was prepared immediately before its introduction.

The animals of the first group were injected with a 1% solution of ethidium bromide in a dose of 0.5-1.0 mg / kg body weight, deeply intramuscularly, once.

To control animals (group 2), a 1% solution of ethidium bromide was injected intramuscularly, at the same dose as the animals of the first group only twice, with an administration interval of 24 hours.

After administration, the general condition of the animals was monitored.

During the entire observation period (20 days), no signs of intoxication were detected in animals of the first group. The very next day, all animals noted a decrease in temperature to normal, an increase in mobility, a gradual restoration of appetite. Two days later, there were no signs of hemoglobinuria. Fifteen days after the administration of ethidium bromide, the clinical picture of pyroplasmosis was not established in all animals; the causative agent of equine pyroplasmosis (P. caballi) was not found in peripheral blood smears.

In animals of the second group, which were injected with a 1% solution of ethidium bromide, only twice the dynamics of changes in the clinical picture of piroplasmosis was similar to that in horses of the first group - the next day the temperature reaction corresponded to physiological norms, also increased appetite and increased physical activity. Two days later, there were no signs of hemoglobinuria. 15 days after the administration of the drug, it can be stated that the horses are in satisfactory condition; the causative agent of pyroplasmosis was not found in blood smears.

However, it should be noted that in one animal 5 hours after the repeated administration of the drug, signs of intoxication were noted - increased sweating, signs of moderate diarrhea, decreased motor activity, and refusal to feed. On the second day after symptomatic and pathogenetic therapy, the condition of the animal can be characterized as satisfactory.

The results of the comparative effectiveness of single and double administration of a 1% aqueous solution of ethidium bromide are presented in the table.

The effectiveness of a 1% solution of ethidium bromide, administered at a dose of 0.5-1.0 mg / kg body weight once, was 100% and was similar to that with the introduction of ethidium bromide in the same doses, but twice with an interval of 24 hours, however in animals with a single administration, in contrast to the administration of a double administration, there were no signs of intoxication.

Table Group No. Number of animals A drug Dose mg / kg Multiplicity of introduction The number of recovered Efficiency% 1 8 Ethidium bromide 0.5-1.0 Once 8 100 2 8 Ethidium bromide 0.5-1.0 Twice, with an interval of 24 hours 8 100

Thus, reintroduction of ethidium bromide in horses with pyroplasmosis is inappropriate.

The use of this method of treatment of equine pyroplasmosis reduces the toxicity of ethidium bromide by reducing the frequency of administration.

Claims (1)

A method of treating equine pyroplasmosis, comprising intramuscular administration of ethidium bromide at a dose of 0.5-1.0 mg / kg body weight, characterized in that the horses are given ethidium bromide once.