RU2015112578A - Локусы fad3 для выполнения операций и соответствующие связывающиеся со специфическими сайтами-мишенями белки, способные к вызову направленных разрывов - Google Patents
Локусы fad3 для выполнения операций и соответствующие связывающиеся со специфическими сайтами-мишенями белки, способные к вызову направленных разрывов Download PDFInfo
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Abstract
1. Способ модификации генома клетки, включающий: расщепление, сайт-специфическим образом, сайта-мишени в гене FAD3 в клетке с созданием, таким образом, разрыва в гене FAD3; причем ген FAD3 подвергается модификации после разрыва.2. Способ по п. 1, причем способ, кроме того, включает интеграцию в разрыв представляющей интерес последовательности нуклеиновой кислоты.3. Способ по п. 1 или 2, причем геном FAD3 является ген FAD3A, FAD3A′, FAD3A′′, FAD3C, FAD3C′′ и/или FAD3C′.4. Способ по п. 1 или 2, причем расщепление сайт-специфическим образом включает введение составного белка, включающего ДНК-связывающий домен и расщепляющий домен или полдомена для расщепления, в клетку или полинуклеотида, кодирующего составной белок, причем составной белок связывается со специфичностью в отношении сайта-мишени и расщепляет в и вблизи сайте(а)-мишени с созданием таким образом разрыва.5. Способ по п. 4, причем ДНК-связывающий домен выбирают из группы, состоящей из ДНК-связывающего домена мегануклеазы, ДНК-связывающего домена «лейциновая молния», ДНК-связывающего домена подобного активатору транскрипции белка (TAL), РНК-направляемой системы CRISPR-Cas9, рекомбиназы, ДНК-связывающего домена белка с цинковыми пальцами и химерных комбинаций любых из вышеизложенных доменов.6. Способ по п. 4, причем расщепляющий домен или полдомена для расщепления выбирают из группы, состоящей из полдомена для расщепления из эндонуклеазы рестрикции типа IIS, полдомена для расщепления из эндонуклеазы FokI, полдомена для расщепления из эндонуклеазы StsI и хоминг-нуклеазы.7. Способ по п. 4, причем составным белком является нуклеаза на основе цинковых пальцев.8. Способ по п. 7, причем нуклеаза на основе цинковых пальцев включает от трех до шести доменов «цинковые пальцы», при этом
Claims (21)
1. Способ модификации генома клетки, включающий: расщепление, сайт-специфическим образом, сайта-мишени в гене FAD3 в клетке с созданием, таким образом, разрыва в гене FAD3; причем ген FAD3 подвергается модификации после разрыва.
2. Способ по п. 1, причем способ, кроме того, включает интеграцию в разрыв представляющей интерес последовательности нуклеиновой кислоты.
3. Способ по п. 1 или 2, причем геном FAD3 является ген FAD3A, FAD3A′, FAD3A′′, FAD3C, FAD3C′′ и/или FAD3C′.
4. Способ по п. 1 или 2, причем расщепление сайт-специфическим образом включает введение составного белка, включающего ДНК-связывающий домен и расщепляющий домен или полдомена для расщепления, в клетку или полинуклеотида, кодирующего составной белок, причем составной белок связывается со специфичностью в отношении сайта-мишени и расщепляет в и вблизи сайте(а)-мишени с созданием таким образом разрыва.
5. Способ по п. 4, причем ДНК-связывающий домен выбирают из группы, состоящей из ДНК-связывающего домена мегануклеазы, ДНК-связывающего домена «лейциновая молния», ДНК-связывающего домена подобного активатору транскрипции белка (TAL), РНК-направляемой системы CRISPR-Cas9, рекомбиназы, ДНК-связывающего домена белка с цинковыми пальцами и химерных комбинаций любых из вышеизложенных доменов.
6. Способ по п. 4, причем расщепляющий домен или полдомена для расщепления выбирают из группы, состоящей из полдомена для расщепления из эндонуклеазы рестрикции типа IIS, полдомена для расщепления из эндонуклеазы FokI, полдомена для расщепления из эндонуклеазы StsI и хоминг-нуклеазы.
7. Способ по п. 4, причем составным белком является нуклеаза на основе цинковых пальцев.
8. Способ по п. 7, причем нуклеаза на основе цинковых пальцев включает от трех до шести доменов «цинковые пальцы», при этом каждый домен «цинковые пальцы» включает распознающий спиральный участок, причем белок с цинковыми пальцами включает распознающие спиральные участки, упорядоченные и продемонстрированные в одном ряду таблицы 3.
9. Способ по п. 1 или 2, причем расщепление сайт-специфическим образом является специфическим в отношении некоторых, но не всех копий FAD3A, FAD3A′, FAD3A′′, FAD3C, FAD3C′ и/или FAD3C′.
10. Способ по п. 1 или 2, причем сайт-мишень выбирают из группы, состоящей из SEQ ID NO: 20-23, SEQ ID NO: 25-38, SEQ ID NO: 40-45, SEQ ID NO: 47 и SEQ ID NO: 49.
11. Способ по п. 1 или 2, причем клеткой является клетка растения, грибов, бактерий или водорослей.
12. Способ по п. 11, причем клеткой растения является клетка однодольного растения или клетка двудольного растения.
13. Способ по п. 12, причем растение выбирают из группы, состоящей из Brassica sp., Brassica napus, Brassica rapa, Brassica juencea, Brassica oleracea, Brassica nigra, Zea sp., Zea mays, Glycine sp., Glycine max, Triticum sp., Triticum aestivum, Oryza sp., Oryza sativa, Triticae sp., Triticae triticum, Heliantheas sp., Heliantheas helianthus, Gossypium sp., Gossypium hirsutum и Hordeum vulgar.
14. Способ по п. 2, причем представляющую интерес последовательность нуклеиновой кислоты выбирают из группы, состоящей из последовательности, включающей сайт-мишень для связывания ДНК-связывающего домена, одного или более генов устойчивости к инсектицидам, одного или более генов устойчивости к гербицидам, одного или более генов эффективности потребления азота, одного или более генов эффективности потребления воды, одного или более генов пищевой ценности, одного или более генов ДНК-связывающих белков, одного или более генов селектируемых маркеров и их комбинации.
15. Клетка, семя или растение, включающее клетку, модифицированную(ая) в соответствии со способом по любому из пп. 1-14.
16. Клетка, семя или растение по п. 15, причем клеткой, семенем или растением является трансгенная(ое) клетка, семя или растение, включающая(ее) представляющую интерес нуклеотидную последовательность, интегрированную в одну или более копий гена FAD3A, FAD3A′, FAD3A′′, FAD3C, FAD3C′′ и/или FAD3C′.
17. Клетка, семя или растение по п. 16, причем нуклеотидная последовательность является гетерологичной или гомологичной по отношению к клетке.
18. Клетка, семя или растение по п. 17, причем гомологичная последовательность включает по меньшей мере один однонуклеотидный полиморфизм.
19. Клетка, семя или растение по п. 16 или 17, причем последовательность нуклеиновой кислоты интегрируется в или вблизи сайте(а)-мишени, выбираемого из группы, состоящей из SEQ ID NO: 20-23, SEQ ID NO: 25-38, SEQ ID NO: 40-45, SEQ ID NO: 47 и SEQ ID NO: 49.
20. Сайт-специфическая нуклеаза на основе цинковых пальцев, которая расщепляет в или вблизи сайте(а)-мишени нуклеиновой кислоты, выбираемого из группы, состоящей из SEQ ID NO: 20-23, SEQ ID NO: 25-38, SEQ ID NO: 40-45, SEQ ID NO: 47 и SEQ ID NO: 49.
21. Нуклеаза на основе цинковых пальцев по п. 20, причем нуклеаза на основе цинковых пальцев включает от трех до шести доменов «цинковые пальцы», при этом каждый домен «цинковые пальцы» включает распознающий спиральный участок, причем белок с цинковыми пальцами включает распознающие спиральные участки, упорядоченные и продемонстрированные в одном ряду таблицы 3.
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| Application Number | Priority Date | Filing Date | Title |
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| US201261697854P | 2012-09-07 | 2012-09-07 | |
| US61/697,854 | 2012-09-07 | ||
| US201361820260P | 2013-05-07 | 2013-05-07 | |
| US61/820,260 | 2013-05-07 | ||
| PCT/US2013/058267 WO2014039684A1 (en) | 2012-09-07 | 2013-09-05 | Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2769284C2 (ru) * | 2017-07-10 | 2022-03-30 | Дюммен Груп Б.В. | Роза, устойчивая к мучнистой росе |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP3613852A3 (en) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| EA038924B1 (ru) | 2012-05-25 | 2021-11-10 | Те Риджентс Оф Те Юниверсити Оф Калифорния | Способы и композиции рнк-специфической модификации днк-мишени и рнк-специфической модуляции транскрипции |
| CN105264067B (zh) * | 2012-09-07 | 2020-11-10 | 美国陶氏益农公司 | Fad3性能基因座及相应的能够诱导靶向断裂的靶位点特异性结合蛋白 |
| UA119135C2 (uk) * | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
| CN105441440B (zh) | 2012-10-23 | 2020-12-15 | 基因工具股份有限公司 | 包含特异于靶dna的向导rna和cas蛋白质编码核酸或cas蛋白质的用于切割靶dna的组合物及其用途 |
| KR102531576B1 (ko) | 2012-12-06 | 2023-05-11 | 시그마-알드리치 컴퍼니., 엘엘씨 | Crispr-기초된 유전체 변형과 조절 |
| AU2014235794A1 (en) | 2013-03-14 | 2015-10-22 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
| EP2981166B1 (en) | 2013-04-05 | 2020-09-09 | Dow AgroSciences LLC | Methods and compositions for integration of an exogenous sequence within the genome of plants |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| DK3066201T3 (en) | 2013-11-07 | 2018-06-06 | Editas Medicine Inc | CRISPR-RELATED PROCEDURES AND COMPOSITIONS WITH LEADING GRADES |
| US20150166982A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting pi3k point mutations |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| CN107250148B (zh) | 2014-12-03 | 2021-04-16 | 安捷伦科技有限公司 | 具有化学修饰的指导rna |
| KR102648489B1 (ko) | 2015-04-06 | 2024-03-15 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | Crispr/cas-매개 유전자 조절을 위한 화학적으로 변형된 가이드 rna |
| WO2017023803A1 (en) | 2015-07-31 | 2017-02-09 | Regents Of The University Of Minnesota | Modified cells and methods of therapy |
| US10837024B2 (en) * | 2015-09-17 | 2020-11-17 | Cellectis | Modifying messenger RNA stability in plant transformations |
| CN108368516A (zh) | 2015-10-06 | 2018-08-03 | 基础科学研究院 | 用于从原生质体生成整个植物的方法 |
| BR112018007022A2 (pt) * | 2015-10-08 | 2018-10-16 | Simplot Co J R | cultivar de batata y9 |
| JP7109784B2 (ja) | 2015-10-23 | 2022-08-01 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 遺伝子編集のための進化したＣａｓ９蛋白質 |
| WO2017134601A1 (en) * | 2016-02-02 | 2017-08-10 | Cellectis | Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| CN107784200B (zh) * | 2016-08-26 | 2020-11-06 | 深圳华大生命科学研究院 | 一种筛选新型CRISPR-Cas系统的方法和装置 |
| IL247752A0 (en) * | 2016-09-11 | 2016-11-30 | Yeda Res & Dev | Compositions and methods for modulating gene expression for site-directed mutagenesis |
| WO2018071868A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
| CN109689693B (zh) * | 2016-11-03 | 2022-06-28 | 深圳华大生命科学研究院 | 提高基因编辑效率的方法和系统 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| CN106591293A (zh) * | 2016-12-28 | 2017-04-26 | 贵州省草业研究所 | 基于酶切连接从未知基因组中分离已知序列侧翼序列的方法 |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| US11390876B2 (en) | 2018-03-09 | 2022-07-19 | Pioneer Hi-Bred International, Inc. | Compositions and methods for modification of fatty acids in soybean |
| EP3578658A1 (en) * | 2018-06-08 | 2019-12-11 | Johann Wolfgang Goethe-Universität Frankfurt | Method for generating a gene editing vector with fixed guide rna pairs |
| CN109868283B (zh) * | 2019-02-21 | 2021-07-20 | 浙江农林大学 | 一种评估CRISPR/Cas9基因编辑效率或脱靶频率的方法 |
| MX2021011426A (es) | 2019-03-19 | 2022-03-11 | Broad Inst Inc | Metodos y composiciones para editar secuencias de nucleótidos. |
| CN111378721B (zh) * | 2020-04-16 | 2023-06-23 | 广西壮族自治区水产科学研究院 | 凡纳滨对虾耐亚硝酸盐氮性状相关的分子标记及其筛选 |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| AU2021271086A1 (en) * | 2020-05-13 | 2022-12-15 | Nunhems B.V. | Method for obtaining mutant plants by targeted mutagenesis |
| CN112813081B (zh) * | 2021-02-23 | 2022-10-28 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | 一种获得高含量亚油酸水稻株系的方法 |
| DE112022001376T5 (de) | 2021-03-05 | 2024-03-14 | Denso Corporation | Spurbestimmungsvorrichtung, spurwechselbestimmungsprogramm und datenstruktur |
| AU2022343300A1 (en) | 2021-09-10 | 2024-04-18 | Agilent Technologies, Inc. | Guide rnas with chemical modification for prime editing |
Family Cites Families (153)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US789538A (en) | 1904-11-11 | 1905-05-09 | Colin E Ham | Dumb-bell. |
| US1173508A (en) | 1913-10-27 | 1916-02-29 | Oscar F Heartwell Jr | Ironing-board. |
| US4727028A (en) | 1981-06-22 | 1988-02-23 | Eli Lilly And Company | Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof |
| US4693977A (en) | 1982-08-23 | 1987-09-15 | Queen's University At Kingston | Enzyme immobilization for producing cephalosporin antibiotics |
| US4536475A (en) | 1982-10-05 | 1985-08-20 | Phytogen | Plant vector |
| US4535060A (en) | 1983-01-05 | 1985-08-13 | Calgene, Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use |
| US5352605A (en) | 1983-01-17 | 1994-10-04 | Monsanto Company | Chimeric genes for transforming plant cells using viral promoters |
| NL8300698A (nl) | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
| US5428147A (en) | 1983-04-15 | 1995-06-27 | Mycogen Plant Science, Inc. | Octopine T-DNA promoters |
| NZ207765A (en) | 1983-04-15 | 1987-03-06 | Lubrizol Genetics Inc | Plant expression of transferred dna(t-dna)from plasmids associated with agrobacterium sp |
| US4940840A (en) | 1984-03-26 | 1990-07-10 | Dna Plant Technology Corporation | Novel chitinase-producing bacteria and plants |
| US5447858A (en) | 1984-04-13 | 1995-09-05 | Mycogen Plant Sciences, Inc. | Heat shock promoter and gene |
| US5420034A (en) | 1986-07-31 | 1995-05-30 | Calgene, Inc. | Seed-specific transcriptional regulation |
| US4943674A (en) | 1987-05-26 | 1990-07-24 | Calgene, Inc. | Fruit specific transcriptional factors |
| US5753475A (en) | 1985-01-17 | 1998-05-19 | Calgene, Inc. | Methods and compositions for regulated transcription and expression of heterologous genes |
| US4886937A (en) | 1985-05-20 | 1989-12-12 | North Carolina State University | Method for transforming pine |
| US4940835A (en) | 1985-10-29 | 1990-07-10 | Monsanto Company | Glyphosate-resistant plants |
| US4810648A (en) | 1986-01-08 | 1989-03-07 | Rhone Poulenc Agrochimie | Haloarylnitrile degrading gene, its use, and cells containing the gene |
| ES2018274T5 (es) | 1986-03-11 | 1996-12-16 | Plant Genetic Systems Nv | Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica. |
| US4975374A (en) | 1986-03-18 | 1990-12-04 | The General Hospital Corporation | Expression of wild type and mutant glutamine synthetase in foreign hosts |
| ATE87032T1 (de) | 1986-12-05 | 1993-04-15 | Ciba Geigy Ag | Verbessertes verfahren zur transformation von pflanzlichen protoplasten. |
| US5015580A (en) | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
| US5359142A (en) | 1987-01-13 | 1994-10-25 | Monsanto Company | Method for enhanced expression of a protein |
| US5322938A (en) | 1987-01-13 | 1994-06-21 | Monsanto Company | DNA sequence for enhancing the efficiency of transcription |
| EP0333033A1 (en) | 1988-03-09 | 1989-09-20 | Meiji Seika Kaisha Ltd. | Glutamine synthesis gene and glutamine synthetase |
| US5416011A (en) | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
| US5302523A (en) | 1989-06-21 | 1994-04-12 | Zeneca Limited | Transformation of plant cells |
| US5501967A (en) | 1989-07-26 | 1996-03-26 | Mogen International, N.V./Rijksuniversiteit Te Leiden | Process for the site-directed integration of DNA into the genome of plants |
| US7705215B1 (en) | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US5550318A (en) | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6051753A (en) | 1989-09-07 | 2000-04-18 | Calgene, Inc. | Figwort mosaic virus promoter and uses |
| DE69033628T2 (de) | 1989-10-31 | 2001-04-19 | Monsanto Co | Promotor für transgene Pflanzen |
| US5641876A (en) | 1990-01-05 | 1997-06-24 | Cornell Research Foundation, Inc. | Rice actin gene and promoter |
| US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
| JP3209744B2 (ja) | 1990-01-22 | 2001-09-17 | デカルブ・ジェネティクス・コーポレーション | 結実能力のある遺伝子変換コーン |
| US6403865B1 (en) | 1990-08-24 | 2002-06-11 | Syngenta Investment Corp. | Method of producing transgenic maize using direct transformation of commercially important genotypes |
| US5633435A (en) | 1990-08-31 | 1997-05-27 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases |
| US5266317A (en) | 1990-10-04 | 1993-11-30 | University Of Georgia Research Foundation, Inc. | Insect-specific paralytic neurotoxin genes for use in biological insect control: methods and compositions |
| US5384253A (en) | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
| GB9104617D0 (en) | 1991-03-05 | 1991-04-17 | Nickerson Int Seed | Pest control |
| GB9115909D0 (en) | 1991-07-23 | 1991-09-04 | Nickerson Int Seed | Recombinant dna |
| DK39692D0 (da) | 1992-03-25 | 1992-03-25 | Danisco | Biologisk materiale |
| US5487994A (en) | 1992-04-03 | 1996-01-30 | The Johns Hopkins University | Insertion and deletion mutants of FokI restriction endonuclease |
| US5436150A (en) | 1992-04-03 | 1995-07-25 | The Johns Hopkins University | Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease |
| US5356802A (en) | 1992-04-03 | 1994-10-18 | The Johns Hopkins University | Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease |
| ATE398679T1 (de) | 1992-07-07 | 2008-07-15 | Japan Tobacco Inc | Verfahren zur transformation einer monokotyledon pflanze |
| EP0652965A1 (en) | 1992-07-27 | 1995-05-17 | Pioneer Hi-Bred International, Inc. | An improved method of agrobacterium-mediated transformation of cultured soybean cells |
| US5607914A (en) | 1993-01-13 | 1997-03-04 | Pioneer Hi-Bred International, Inc. | Synthetic antimicrobial peptides |
| US6118047A (en) | 1993-08-25 | 2000-09-12 | Dekalb Genetic Corporation | Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction |
| US5362865A (en) | 1993-09-02 | 1994-11-08 | Monsanto Company | Enhanced expression in plants using non-translated leader sequences |
| US5580852A (en) | 1993-12-17 | 1996-12-03 | Pioneer Hi-Bred International, Inc. | Derivatives of tachyplesin having inhibitory activity towards plant pathogenic fungi |
| CA2681922C (en) | 1994-01-18 | 2012-05-15 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
| US6242568B1 (en) | 1994-01-18 | 2001-06-05 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
| US6140466A (en) | 1994-01-18 | 2000-10-31 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
| EP2022856B1 (en) | 1994-08-20 | 2011-09-14 | Gendaq Limited | Improvements in or relating to binding proteins for recognition of DNA |
| GB9824544D0 (en) | 1998-11-09 | 1999-01-06 | Medical Res Council | Screening system |
| US5789538A (en) | 1995-02-03 | 1998-08-04 | Massachusetts Institute Of Technology | Zinc finger proteins with high affinity new DNA binding specificities |
| US5994627A (en) | 1995-03-31 | 1999-11-30 | Common Wealth Scientific And Industrial Research Organisation | Genetic sequences conferring nematode resistance in plants and uses therefor |
| US5693512A (en) | 1996-03-01 | 1997-12-02 | The Ohio State Research Foundation | Method for transforming plant tissue by sonication |
| US5850019A (en) | 1996-08-06 | 1998-12-15 | University Of Kentucky Research Foundation | Promoter (FLt) for the full-length transcript of peanut chlorotic streak caulimovirus (PCLSV) and expression of chimeric genes in plants |
| US5925523A (en) | 1996-08-23 | 1999-07-20 | President & Fellows Of Harvard College | Intraction trap assay, reagents and uses thereof |
| JPH11514534A (ja) | 1996-09-05 | 1999-12-14 | ユニリーバー・ナームローゼ・ベンノートシヤープ | 乳酸菌から誘導される塩誘発プロモーターおよび所望のタンパク質産生のための乳酸菌でのその使用 |
| HUP0000922A3 (en) | 1997-01-20 | 2002-03-28 | Plant Genetic Systems Nv | Pathogen-induced plant promoters |
| US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
| GB2338237B (en) | 1997-02-18 | 2001-02-28 | Actinova Ltd | In vitro peptide or protein expression library |
| GB9703369D0 (en) | 1997-02-18 | 1997-04-09 | Lindqvist Bjorn H | Process |
| US5922564A (en) | 1997-02-24 | 1999-07-13 | Performance Plants, Inc. | Phosphate-deficiency inducible promoter |
| GB9710807D0 (en) | 1997-05-23 | 1997-07-23 | Medical Res Council | Nucleic acid binding proteins |
| GB9710809D0 (en) | 1997-05-23 | 1997-07-23 | Medical Res Council | Nucleic acid binding proteins |
| US6087166A (en) | 1997-07-03 | 2000-07-11 | Basf Aktiengesellschaft | Transcriptional activators with graded transactivation potential |
| US6410248B1 (en) | 1998-01-30 | 2002-06-25 | Massachusetts Institute Of Technology | General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites |
| CA2319079A1 (en) | 1998-02-20 | 1999-08-26 | Zeneca Limited | Pollen specific promoter |
| ES2270227T3 (es) | 1998-02-26 | 2007-04-01 | Pioneer Hi-Bred International, Inc. | Promotor met-1 del maiz. |
| CA2315549A1 (en) | 1998-02-26 | 1999-09-02 | Pioneer Hi-Bred International, Inc. | Family of maize pr-1 genes and promoters |
| EP1060261B1 (en) | 1998-03-02 | 2010-05-05 | Massachusetts Institute of Technology | Poly zinc finger proteins with improved linkers |
| US6635806B1 (en) | 1998-05-14 | 2003-10-21 | Dekalb Genetics Corporation | Methods and compositions for expression of transgenes in plants |
| US6307123B1 (en) | 1998-05-18 | 2001-10-23 | Dekalb Genetics Corporation | Methods and compositions for transgene identification |
| US6140815A (en) | 1998-06-17 | 2000-10-31 | Dover Instrument Corporation | High stability spin stand platform |
| JP2000083680A (ja) | 1998-07-16 | 2000-03-28 | Nippon Paper Industries Co Ltd | 光誘導型プロモ―タ―の制御下に置かれた不定芽再分化遺伝子を選抜マ―カ―遺伝子とする植物への遺伝子導入方法及びこれに用いる植物への遺伝子導入用ベクタ― |
| US6140081A (en) | 1998-10-16 | 2000-10-31 | The Scripps Research Institute | Zinc finger binding domains for GNN |
| US6534261B1 (en) | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
| US7070934B2 (en) | 1999-01-12 | 2006-07-04 | Sangamo Biosciences, Inc. | Ligand-controlled regulation of endogenous gene expression |
| US6453242B1 (en) | 1999-01-12 | 2002-09-17 | Sangamo Biosciences, Inc. | Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites |
| US6599692B1 (en) | 1999-09-14 | 2003-07-29 | Sangamo Bioscience, Inc. | Functional genomics using zinc finger proteins |
| EP1141346A2 (en) | 1999-01-14 | 2001-10-10 | Monsanto Co. | Soybean transformation method |
| US6794136B1 (en) | 2000-11-20 | 2004-09-21 | Sangamo Biosciences, Inc. | Iterative optimization in the design of binding proteins |
| US7030215B2 (en) | 1999-03-24 | 2006-04-18 | Sangamo Biosciences, Inc. | Position dependent recognition of GNN nucleotide triplets by zinc fingers |
| US6429357B1 (en) | 1999-05-14 | 2002-08-06 | Dekalb Genetics Corp. | Rice actin 2 promoter and intron and methods for use thereof |
| US6194636B1 (en) | 1999-05-14 | 2001-02-27 | Dekalb Genetics Corp. | Maize RS324 promoter and methods for use thereof |
| US6207879B1 (en) | 1999-05-14 | 2001-03-27 | Dekalb Genetics Corporation | Maize RS81 promoter and methods for use thereof |
| US6232526B1 (en) | 1999-05-14 | 2001-05-15 | Dekalb Genetics Corp. | Maize A3 promoter and methods for use thereof |
| US6677503B1 (en) | 1999-06-23 | 2004-01-13 | Pioneer Hi-Bred International, Inc. | Sunflower anti-pathogene proteins and genes and their uses |
| CA2284246A1 (en) * | 1999-10-01 | 2001-04-01 | Agriculture And Agrifood Canada Of Agriculture And Agri-Food | Plant fatty acid desaturases and alleles therefor |
| WO2001040798A2 (en) | 1999-12-06 | 2001-06-07 | Sangamo Biosciences, Inc. | Methods of using randomized libraries of zinc finger proteins for the identification of gene function |
| EP1248850A2 (en) | 2000-01-21 | 2002-10-16 | Pioneer Hi-Bred International, Inc. | Root-preferred promoter elements and methods of use |
| WO2001059450A2 (en) | 2000-02-08 | 2001-08-16 | Sangamo Biosciences, Inc. | Cells expressing zinc finger protein for drug discovery |
| US20020061512A1 (en) | 2000-02-18 | 2002-05-23 | Kim Jin-Soo | Zinc finger domains and methods of identifying same |
| US6388170B1 (en) | 2000-04-07 | 2002-05-14 | University Of Kentucky Research Foundation | Bidirectional promoters and methods related thereto |
| WO2001088197A2 (en) | 2000-05-16 | 2001-11-22 | Massachusetts Institute Of Technology | Methods and compositions for interaction trap assays |
| JP2002060786A (ja) | 2000-08-23 | 2002-02-26 | Kao Corp | 硬質表面用殺菌防汚剤 |
| US7067317B2 (en) | 2000-12-07 | 2006-06-27 | Sangamo Biosciences, Inc. | Regulation of angiogenesis with zinc finger proteins |
| US7022826B2 (en) | 2001-02-26 | 2006-04-04 | The Regents Of The University Of California | Non-oligomerizing fluorescent proteins |
| GB0108491D0 (en) | 2001-04-04 | 2001-05-23 | Gendaq Ltd | Engineering zinc fingers |
| US20040224385A1 (en) | 2001-08-20 | 2004-11-11 | Barbas Carlos F | Zinc finger binding domains for cnn |
| US7262054B2 (en) | 2002-01-22 | 2007-08-28 | Sangamo Biosciences, Inc. | Zinc finger proteins for DNA binding and gene regulation in plants |
| US8106255B2 (en) * | 2002-01-23 | 2012-01-31 | Dana Carroll | Targeted chromosomal mutagenasis using zinc finger nucleases |
| AU2003218382B2 (en) | 2002-03-21 | 2007-12-13 | Sangamo Therapeutics, Inc. | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
| US7361635B2 (en) | 2002-08-29 | 2008-04-22 | Sangamo Biosciences, Inc. | Simultaneous modulation of multiple genes |
| AU2003298574B2 (en) | 2002-09-05 | 2008-04-24 | California Institute Of Technology | Use of chimeric nucleases to stimulate gene targeting |
| ES2390765T3 (es) | 2003-02-11 | 2012-11-16 | Dow Agrosciences Llc | Genes FAD2 y FAD3 Alterados en Brassica y la detección asistida por marcadores moleculares de éstos |
| BRPI0409816B8 (pt) | 2003-04-29 | 2022-12-06 | Pioneer Hi Bred Int | Genes de glifosato-n-acetiltransferase (gat), construtos os compreendendo, célula bacteriana, polipeptídeo tendo atividade de gat, bem como método para a produção de uma planta transgênica resistente ao glifosato e métodos para controlar ervas daninhas em um campo contendo uma safra |
| US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| US8409861B2 (en) | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
| KR100537955B1 (ko) | 2003-10-29 | 2005-12-20 | 학교법인고려중앙학원 | 꽃가루 특이적 유전자 발현 프로모터 |
| US7972854B2 (en) | 2004-02-05 | 2011-07-05 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| CA2562193A1 (en) | 2004-04-08 | 2005-10-27 | Sangamo Biosciences, Inc. | Treatment of neuropathic pain with zinc finger proteins |
| EP1732614B1 (en) | 2004-04-08 | 2008-12-24 | Sangamo Biosciences Inc. | Compositions for treating neuropathic and neurodegenerative conditions |
| CA2561565C (en) * | 2004-04-08 | 2013-11-26 | Sangamo Biosciences, Inc. | Methods for repression of phospholamban gene and modulating cardiac contractility |
| SI2308977T2 (sl) | 2004-04-30 | 2017-07-31 | Dow Agrosciences Llc | Novi geni z rezistenco na herbicide |
| EP1846560A4 (en) * | 2005-02-09 | 2009-02-11 | Bioriginal Food & Science Corp | NEW MEMBERS OF THE OMEGA-3 FATTY ACID DESATURASE FAMILY AND USES THEREOF |
| EP1877583A2 (en) | 2005-05-05 | 2008-01-16 | Arizona Board of Regents on behalf of the Unversity of Arizona | Sequence enabled reassembly (seer) - a novel method for visualizing specific dna sequences |
| AP2693A (en) * | 2005-05-27 | 2013-07-16 | Monsanto Technology Llc | Soybean event MON89788 and methods for detection thereof |
| KR101419729B1 (ko) * | 2005-07-26 | 2014-07-17 | 상가모 바이오사이언스 인코포레이티드 | 외래 핵산 서열의 표적화된 통합 및 발현 |
| ES2612119T3 (es) | 2005-10-28 | 2017-05-12 | Dow Agrosciences Llc | Nuevos genes de resistencia a herbicidas |
| CA2651494C (en) | 2006-05-25 | 2015-09-29 | Sangamo Biosciences, Inc. | Engineered cleavage half-domains |
| CN105296527B (zh) | 2006-08-11 | 2020-11-27 | 陶氏益农公司 | 锌指核酸酶介导的同源重组 |
| WO2008076290A2 (en) | 2006-12-14 | 2008-06-26 | Dow Agrosciences Llc | Optimized non-canonical zinc finger proteins |
| CA2700231C (en) | 2007-09-27 | 2018-09-18 | Sangamo Biosciences, Inc. | Rapid in vivo identification of biologically active nucleases |
| CA2937438C (en) | 2007-09-27 | 2020-07-07 | Dow Agrosciences Llc | Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes |
| AU2008317354B2 (en) | 2007-10-25 | 2014-04-10 | Ospedale San Raffaele S.R.L. | Methods and compositions for targeted integration |
| AU2009238629C1 (en) | 2008-04-14 | 2015-04-30 | Sangamo Therapeutics, Inc. | Linear donor constructs for targeted integration |
| US8703489B2 (en) | 2008-08-22 | 2014-04-22 | Sangamo Biosciences, Inc. | Methods and compositions for targeted single-stranded cleavage and targeted integration |
| RU2557316C2 (ru) * | 2008-11-04 | 2015-07-20 | ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи | Brassica juncea КАЧЕСТВА ОМЕГА-9 |
| JP2012511926A (ja) | 2008-12-17 | 2012-05-31 | ダウ・アグロサイエンス・エル・エル・シー | Ｚｐ１５遺伝子座内への標的組込み |
| EP2206723A1 (en) | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
| US20110239315A1 (en) | 2009-01-12 | 2011-09-29 | Ulla Bonas | Modular dna-binding domains and methods of use |
| KR102262704B1 (ko) | 2009-08-11 | 2021-06-09 | 상가모 테라퓨틱스, 인코포레이티드 | 표적화 변형에 대한 동형접합성 유기체 |
| US10340778B2 (en) | 2009-10-19 | 2019-07-02 | Qm Power, Inc. | Parallel magnetic circuit motor |
| MY174390A (en) * | 2009-10-22 | 2020-04-15 | Sangamo Biosciences Inc | Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis |
| CN102712911B (zh) | 2009-11-20 | 2016-01-13 | 拜尔作物科学公司 | 包含突变fad3等位基因的芸苔属植物 |
| PL2510096T5 (pl) | 2009-12-10 | 2018-06-29 | Regents Of The University Of Minnesota | Modyfikacja DNA zależna od efektora TAL |
| CN102433331B (zh) * | 2009-12-28 | 2013-05-08 | 华中农业大学 | 一种甘蓝型油菜低亚麻酸分子标记及其制备方法与应用 |
| GEP20176628B (en) | 2010-01-22 | 2017-02-27 | Sangamo Biosciences Inc | Targeted genomic alteration |
| EP2615106B1 (en) | 2010-02-08 | 2018-04-25 | Sangamo Therapeutics, Inc. | Engineered cleavage half-domains |
| CA2788850C (en) | 2010-02-09 | 2019-06-25 | Sangamo Biosciences, Inc. | Targeted genomic modification with partially single-stranded donor molecules |
| CA2798988C (en) | 2010-05-17 | 2020-03-10 | Sangamo Biosciences, Inc. | Tal-effector (tale) dna-binding polypeptides and uses thereof |
| JP6349261B2 (ja) | 2012-02-01 | 2018-06-27 | ダウ アグロサイエンシィズ エルエルシー | 合成アブラナ属由来葉緑体輸送ペプチド |
| RU2650819C2 (ru) * | 2012-05-07 | 2018-04-17 | Сангамо Терапьютикс, Инк. | Способы и композиции для опосредованной нуклеазой направленной интеграции трансгенов |
| UA118090C2 (uk) * | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив |
| AR092482A1 (es) * | 2012-09-07 | 2015-04-22 | Dow Agrosciences Llc | Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas |
| UA119135C2 (uk) * | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
| CN105264067B (zh) * | 2012-09-07 | 2020-11-10 | 美国陶氏益农公司 | Fad3性能基因座及相应的能够诱导靶向断裂的靶位点特异性结合蛋白 |
| JP5937635B2 (ja) | 2014-03-28 | 2016-06-22 | ファナック株式会社 | 電磁接触器の溶着検出機能を有するモータ駆動装置 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2769284C2 (ru) * | 2017-07-10 | 2022-03-30 | Дюммен Груп Б.В. | Роза, устойчивая к мучнистой росе |
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