RU2014146286A - Способ осуществления цифровой пцр - Google Patents
Способ осуществления цифровой пцр Download PDFInfo
- Publication number
- RU2014146286A RU2014146286A RU2014146286A RU2014146286A RU2014146286A RU 2014146286 A RU2014146286 A RU 2014146286A RU 2014146286 A RU2014146286 A RU 2014146286A RU 2014146286 A RU2014146286 A RU 2014146286A RU 2014146286 A RU2014146286 A RU 2014146286A
- Authority
- RU
- Russia
- Prior art keywords
- sample
- nucleic acid
- target nucleic
- ionic concentration
- change
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 30
- 239000000523 sample Substances 0.000 claims abstract 33
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 16
- 102000039446 nucleic acids Human genes 0.000 claims abstract 16
- 108020004707 nucleic acids Proteins 0.000 claims abstract 16
- 239000011248 coating agent Substances 0.000 claims abstract 8
- 238000000576 coating method Methods 0.000 claims abstract 8
- 150000002500 ions Chemical class 0.000 claims abstract 7
- 230000003321 amplification Effects 0.000 claims abstract 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract 6
- 239000000758 substrate Substances 0.000 claims abstract 4
- 239000000126 substance Substances 0.000 claims abstract 3
- 239000008187 granular material Substances 0.000 claims abstract 2
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract 2
- 239000001257 hydrogen Substances 0.000 claims abstract 2
- -1 hydrogen ions Chemical class 0.000 claims abstract 2
- 239000007788 liquid Substances 0.000 claims abstract 2
- 239000000499 gel Substances 0.000 claims 2
- 239000011159 matrix material Substances 0.000 claims 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims 1
- 238000009395 breeding Methods 0.000 claims 1
- 230000001488 breeding effect Effects 0.000 claims 1
- 210000002744 extracellular matrix Anatomy 0.000 claims 1
- 238000005194 fractionation Methods 0.000 claims 1
- 239000000017 hydrogel Substances 0.000 claims 1
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 claims 1
- 238000011002 quantification Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
- C12M1/38—Temperature-responsive control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261635584P | 2012-04-19 | 2012-04-19 | |
| US61/635,584 | 2012-04-19 | ||
| PCT/US2013/032598 WO2013158313A1 (en) | 2012-04-19 | 2013-03-15 | Nucleic acid amplification |
| USPCT/US2013/032598 | 2013-03-15 | ||
| PCT/US2013/037352 WO2013158982A1 (en) | 2012-04-19 | 2013-04-19 | Method of performing digital pcr |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2014146286A true RU2014146286A (ru) | 2016-06-10 |
Family
ID=49384101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2014146286A RU2014146286A (ru) | 2012-04-19 | 2013-04-19 | Способ осуществления цифровой пцр |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10030262B2 (https=) |
| EP (1) | EP2839029B1 (https=) |
| KR (1) | KR20150011359A (https=) |
| CN (1) | CN104487592B (https=) |
| AU (1) | AU2013249123A1 (https=) |
| IN (1) | IN2014DN09784A (https=) |
| RU (1) | RU2014146286A (https=) |
| WO (1) | WO2013158982A1 (https=) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3957398A1 (en) * | 2015-09-29 | 2022-02-23 | Life Technologies Corporation | Systems and methods for performing digital pcr |
| CN108779423B (zh) * | 2016-03-28 | 2021-12-21 | 富士胶片株式会社 | Pcr用容器 |
| WO2017196676A1 (en) * | 2016-05-10 | 2017-11-16 | Life Technologies Corporation | Metal chelation post incorporation detection methods |
| EP3299471B1 (en) * | 2016-09-23 | 2019-10-23 | Roche Diagniostics GmbH | Methods for determining the amount of a nucleic acid of interest in an unprocessed sample |
| BR112019012414A2 (pt) | 2016-12-19 | 2020-02-27 | Quantum-Si Incorporated | Carregamento de moléculas em poços de amostra para análise |
| CN106497774A (zh) * | 2017-01-03 | 2017-03-15 | 京东方科技集团股份有限公司 | 基因测序芯片、基因测序设备及基因测序方法 |
| CN106754312B (zh) * | 2017-01-03 | 2025-01-28 | 京东方科技集团股份有限公司 | 基因测序芯片、基因测序设备及基因测序方法 |
| US20180318839A1 (en) * | 2017-05-03 | 2018-11-08 | Lifeos Genomics Corporation | Incubation Device Having Rotary Mechanism |
| KR102709865B1 (ko) | 2017-05-05 | 2024-09-26 | 퀀텀-에스아이 인코포레이티드 | 생물학적 반응에 있어서 개질된 표면 반응성 및 오손방지 특성을 갖는 기재 |
| JP6921638B2 (ja) | 2017-06-16 | 2021-08-18 | 株式会社東芝 | 核酸検出定量方法、チップ、アッセイキット、核酸検出定量装置及びプログラム |
| CN109504745A (zh) * | 2017-09-12 | 2019-03-22 | 莱弗斯基因股份有限公司 | 分析样品中多重目标核酸检测方法 |
| CN107385103B (zh) * | 2017-09-18 | 2023-04-07 | 星源智（珠海）生物科技有限公司 | 一种非纯化核酸扩增方法及装置 |
| CN110819522B (zh) * | 2018-08-13 | 2023-09-22 | 上海新微技术研发中心有限公司 | 一种数字pcr系统及数字pcr液滴形成方法 |
| CN110819523B (zh) | 2018-08-13 | 2023-08-29 | 上海新微技术研发中心有限公司 | 一种数字pcr系统及数字pcr液滴形成方法 |
| CN111349555B (zh) * | 2018-12-21 | 2023-07-18 | 成都万众壹芯生物科技有限公司 | 一种基于微孔阵列芯片的数字pcr扩增装置及其使用方法 |
| CA3129315A1 (en) | 2019-02-11 | 2020-08-20 | Ultima Genomics, Inc. | Methods for nucleic acid analysis |
| CN112239719A (zh) * | 2019-07-19 | 2021-01-19 | 成都万众壹芯生物科技有限公司 | 基于微孔阵列芯片的数字pcr扩增装置和利用其进行扩增的方法 |
| US11712715B2 (en) | 2019-10-11 | 2023-08-01 | Quantum-Si Incorporated | Surface modification in the vapor phase |
| US12397293B2 (en) | 2019-12-04 | 2025-08-26 | Hewlett-Packard Development Company, L.P. | Sorting a droplet including a biologic sample |
| KR102395365B1 (ko) * | 2020-02-10 | 2022-05-10 | 한국과학기술연구원 | 소수성 플레이트 상에 배열된 복수의 하이드로젤 미세입자를 포함하는 pcr용 반응 칩 |
| CN111500408B (zh) * | 2020-04-24 | 2022-03-22 | 浙江大学 | 在全封闭条件下进行核酸分析的试剂盒、装置及分析方法 |
| EP4157992A4 (en) * | 2020-05-26 | 2024-07-31 | Bio-Rad Laboratories, Inc. | APPARATUS AND METHOD FOR RAPID DIGITAL DETECTION |
| WO2022109622A1 (en) * | 2020-11-23 | 2022-05-27 | Spangler Frank Leo | Transparent heating elements for polymerase chain reaction devices |
| WO2022259182A1 (en) | 2021-06-09 | 2022-12-15 | Ecole Polytechnique Federale De Lausanne (Epfl) | Device to carry out semiconductor-based diagnostics |
| KR102595551B1 (ko) * | 2021-06-23 | 2023-10-30 | (주)옵토레인 | 다중 친수성을 갖는 체외진단칩 |
| KR102537348B1 (ko) * | 2022-08-04 | 2023-05-30 | 한국기계연구원 | 디지털 pcr 분석용 기판 및 이의 제작방법 |
| KR102819404B1 (ko) * | 2022-08-31 | 2025-06-13 | 압티지엠제이 주식회사 | Pcr을 위한 열 발생 장치 및 그 방법 |
| WO2025230778A2 (en) * | 2024-04-30 | 2025-11-06 | Gram Medical Inc. | Method for processing biological samples |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6413792B1 (en) | 2000-04-24 | 2002-07-02 | Eagle Research Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| EP3985125A1 (en) | 2004-06-01 | 2022-04-20 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification |
| WO2007107710A1 (en) | 2006-03-17 | 2007-09-27 | Solexa Limited | Isothermal methods for creating clonal single molecule arrays |
| GB2457851B (en) * | 2006-12-14 | 2011-01-05 | Ion Torrent Systems Inc | Methods and apparatus for measuring analytes using large scale fet arrays |
| US7932034B2 (en) | 2006-12-20 | 2011-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Heat and pH measurement for sequencing of DNA |
| JP5368321B2 (ja) | 2007-03-02 | 2013-12-18 | ディ・エヌ・エイ・エレクトロニクス・リミテッド | 固相ｐＨ検出を用いたｑＰＣＲ |
| US20090053719A1 (en) * | 2007-08-03 | 2009-02-26 | The Chinese University Of Hong Kong | Analysis of nucleic acids by digital pcr |
| US20090286286A1 (en) | 2007-11-06 | 2009-11-19 | Ambergen , Inc. | Methods for controlling amplification |
| WO2009067632A1 (en) | 2007-11-20 | 2009-05-28 | Applied Biosystems Inc. | Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds |
| US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US20120156728A1 (en) | 2010-12-17 | 2012-06-21 | Life Technologies Corporation | Clonal amplification of nucleic acid on solid surface with template walking |
| WO2011106368A2 (en) | 2010-02-23 | 2011-09-01 | Illumina, Inc. | Amplification methods to minimise sequence specific bias |
| EP2539471B1 (en) | 2010-02-26 | 2014-08-06 | Life Technologies Corporation | Method for sequencing using a modified polymerase |
| WO2012036679A1 (en) | 2010-09-15 | 2012-03-22 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
| DE102011054101A1 (de) | 2011-09-30 | 2013-04-04 | Albert-Ludwigs-Universität Freiburg | Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen |
-
2013
- 2013-04-19 AU AU2013249123A patent/AU2013249123A1/en not_active Abandoned
- 2013-04-19 WO PCT/US2013/037352 patent/WO2013158982A1/en not_active Ceased
- 2013-04-19 US US14/395,456 patent/US10030262B2/en active Active
- 2013-04-19 CN CN201380028515.6A patent/CN104487592B/zh not_active Expired - Fee Related
- 2013-04-19 KR KR1020147032455A patent/KR20150011359A/ko not_active Withdrawn
- 2013-04-19 RU RU2014146286A patent/RU2014146286A/ru not_active Application Discontinuation
- 2013-04-19 EP EP13719209.2A patent/EP2839029B1/en not_active Not-in-force
-
2014
- 2014-11-19 IN IN9784DEN2014 patent/IN2014DN09784A/en unknown
-
2018
- 2018-07-23 US US16/043,002 patent/US20180327813A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US10030262B2 (en) | 2018-07-24 |
| AU2013249123A1 (en) | 2014-12-11 |
| US20150099644A1 (en) | 2015-04-09 |
| EP2839029A1 (en) | 2015-02-25 |
| IN2014DN09784A (https=) | 2015-07-31 |
| US20180327813A1 (en) | 2018-11-15 |
| CN104487592A (zh) | 2015-04-01 |
| WO2013158982A1 (en) | 2013-10-24 |
| KR20150011359A (ko) | 2015-01-30 |
| EP2839029B1 (en) | 2017-11-15 |
| CN104487592B (zh) | 2018-08-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA93 | Acknowledgement of application withdrawn (no request for examination) |
Effective date: 20160420 |