IN2014DN09784A - - Google Patents
Info
- Publication number
- IN2014DN09784A IN2014DN09784A IN9784DEN2014A IN2014DN09784A IN 2014DN09784 A IN2014DN09784 A IN 2014DN09784A IN 9784DEN2014 A IN9784DEN2014 A IN 9784DEN2014A IN 2014DN09784 A IN2014DN09784 A IN 2014DN09784A
- Authority
- IN
- India
- Prior art keywords
- nucleic acid
- target nucleic
- sample
- sample volumes
- fractions
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
- C12M1/38—Temperature-responsive control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Abstract
A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present , counting the number of fractions with an amplified target nucleic acid , and determining the quantity of target nucleic acid in the sample.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261635584P | 2012-04-19 | 2012-04-19 | |
PCT/US2013/032598 WO2013158313A1 (en) | 2012-04-19 | 2013-03-15 | Nucleic acid amplification |
PCT/US2013/037352 WO2013158982A1 (en) | 2012-04-19 | 2013-04-19 | Method of performing digital pcr |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2014DN09784A true IN2014DN09784A (en) | 2015-07-31 |
Family
ID=49384101
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN9784DEN2014 IN2014DN09784A (en) | 2012-04-19 | 2014-11-19 |
Country Status (8)
Country | Link |
---|---|
US (2) | US10030262B2 (en) |
EP (1) | EP2839029B1 (en) |
KR (1) | KR20150011359A (en) |
CN (1) | CN104487592B (en) |
AU (1) | AU2013249123A1 (en) |
IN (1) | IN2014DN09784A (en) |
RU (1) | RU2014146286A (en) |
WO (1) | WO2013158982A1 (en) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017059049A1 (en) * | 2015-09-29 | 2017-04-06 | Life Technologies Corporation | Systems and methods for performing digital pcr |
CN108779423B (en) * | 2016-03-28 | 2021-12-21 | 富士胶片株式会社 | PCR container |
WO2017196676A1 (en) * | 2016-05-10 | 2017-11-16 | Life Technologies Corporation | Metal chelation post incorporation detection methods |
EP3299471B1 (en) * | 2016-09-23 | 2019-10-23 | Roche Diagniostics GmbH | Methods for determining the amount of a nucleic acid of interest in an unprocessed sample |
JP7167024B2 (en) * | 2016-12-19 | 2022-11-08 | クアンタム-エスアイ インコーポレイテッド | Loading molecules into sample wells for analysis |
CN106754312A (en) * | 2017-01-03 | 2017-05-31 | 京东方科技集团股份有限公司 | Gene sequencing chip, gene sequencing equipment and gene order surveying method |
CN106497774A (en) * | 2017-01-03 | 2017-03-15 | 京东方科技集团股份有限公司 | Gene sequencing chip, gene sequencing equipment and gene order surveying method |
US20180318839A1 (en) * | 2017-05-03 | 2018-11-08 | Lifeos Genomics Corporation | Incubation Device Having Rotary Mechanism |
BR112019021889A2 (en) | 2017-05-05 | 2020-05-26 | Quantum-Si Incorporated | SUBSTRATES HAVING MODIFIED SURFACE REACTIVITY AND ANTI-SCALING PROPERTIES IN BIOLOGICAL REACTIONS |
JP6921638B2 (en) | 2017-06-16 | 2021-08-18 | 株式会社東芝 | Nucleic acid detection and quantification method, chip, assay kit, nucleic acid detection and quantification device and program |
CN109504745A (en) * | 2017-09-12 | 2019-03-22 | 莱弗斯基因股份有限公司 | Analyze multiple target nucleic acid detection method in sample |
CN107385103B (en) * | 2017-09-18 | 2023-04-07 | 星源智(珠海)生物科技有限公司 | Method and device for amplifying unpurified nucleic acid |
CN110819522B (en) * | 2018-08-13 | 2023-09-22 | 上海新微技术研发中心有限公司 | Digital PCR system and digital PCR liquid drop forming method |
CN110819523B (en) * | 2018-08-13 | 2023-08-29 | 上海新微技术研发中心有限公司 | Digital PCR system and digital PCR liquid drop forming method |
CN111349555B (en) * | 2018-12-21 | 2023-07-18 | 成都万众壹芯生物科技有限公司 | Digital PCR amplification device based on micropore array chip and use method thereof |
CN112239719A (en) * | 2019-07-19 | 2021-01-19 | 成都万众壹芯生物科技有限公司 | Digital PCR amplification device based on micropore array chip and method for amplifying by using same |
CA3157505A1 (en) | 2019-10-11 | 2021-04-15 | Quantum-Si Incorporated | Surface modification in the vapor phase |
EP3990661A4 (en) * | 2019-12-04 | 2022-07-27 | Hewlett-Packard Development Company, L.P. | Sorting a droplet including a biologic sample |
KR102395365B1 (en) * | 2020-02-10 | 2022-05-10 | 한국과학기술연구원 | Reaction chip for PCR comprising multiple hydrogel microparticles arranged on a hydrophobic plate |
CN111500408B (en) * | 2020-04-24 | 2022-03-22 | 浙江大学 | Kit, device and analysis method for nucleic acid analysis under totally enclosed conditions |
WO2022109622A1 (en) * | 2020-11-23 | 2022-05-27 | Spangler Frank Leo | Transparent heating elements for polymerase chain reaction devices |
WO2022259182A1 (en) | 2021-06-09 | 2022-12-15 | Ecole Polytechnique Federale De Lausanne (Epfl) | Device to carry out semiconductor-based diagnostics |
KR102537348B1 (en) * | 2022-08-04 | 2023-05-30 | 한국기계연구원 | Digital-PCR Analysis Substrate and Fabrication Method Thereof |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6413792B1 (en) | 2000-04-24 | 2002-07-02 | Eagle Research Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
JP5026958B2 (en) | 2004-06-01 | 2012-09-19 | アリーア サン ディエゴ, インコーポレイテッド | Recombinase polymerase amplification |
US20080009420A1 (en) | 2006-03-17 | 2008-01-10 | Schroth Gary P | Isothermal methods for creating clonal single molecule arrays |
EP2639579B1 (en) | 2006-12-14 | 2016-11-16 | Life Technologies Corporation | Apparatus for measuring analytes using large scale FET arrays |
US7932034B2 (en) | 2006-12-20 | 2011-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Heat and pH measurement for sequencing of DNA |
EP2129792B1 (en) * | 2007-03-02 | 2010-09-01 | DNA Electronics Ltd | Qpcr using an ion-sensitive field effect transistor for ph sensing |
US20090053719A1 (en) * | 2007-08-03 | 2009-02-26 | The Chinese University Of Hong Kong | Analysis of nucleic acids by digital pcr |
US20090286286A1 (en) | 2007-11-06 | 2009-11-19 | Ambergen , Inc. | Methods for controlling amplification |
WO2009067632A1 (en) | 2007-11-20 | 2009-05-28 | Applied Biosystems Inc. | Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds |
US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
WO2011106368A2 (en) | 2010-02-23 | 2011-09-01 | Illumina, Inc. | Amplification methods to minimise sequence specific bias |
EP2808401B1 (en) * | 2010-02-26 | 2016-12-14 | Life Technologies Corporation | Method for sequencing using a modified DNA polymerase |
EP2617061B1 (en) * | 2010-09-15 | 2021-06-30 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
EP2652148B1 (en) | 2010-12-17 | 2016-11-30 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
DE102011054101A1 (en) | 2011-09-30 | 2013-04-04 | Albert-Ludwigs-Universität Freiburg | Method for the spatial arrangement of sample fragments for amplification and immobilization for further derivatizations |
-
2013
- 2013-04-19 WO PCT/US2013/037352 patent/WO2013158982A1/en active Application Filing
- 2013-04-19 US US14/395,456 patent/US10030262B2/en active Active
- 2013-04-19 EP EP13719209.2A patent/EP2839029B1/en not_active Not-in-force
- 2013-04-19 KR KR1020147032455A patent/KR20150011359A/en not_active Application Discontinuation
- 2013-04-19 CN CN201380028515.6A patent/CN104487592B/en not_active Expired - Fee Related
- 2013-04-19 RU RU2014146286A patent/RU2014146286A/en not_active Application Discontinuation
- 2013-04-19 AU AU2013249123A patent/AU2013249123A1/en not_active Abandoned
-
2014
- 2014-11-19 IN IN9784DEN2014 patent/IN2014DN09784A/en unknown
-
2018
- 2018-07-23 US US16/043,002 patent/US20180327813A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP2839029B1 (en) | 2017-11-15 |
KR20150011359A (en) | 2015-01-30 |
CN104487592B (en) | 2018-08-28 |
AU2013249123A1 (en) | 2014-12-11 |
US20180327813A1 (en) | 2018-11-15 |
CN104487592A (en) | 2015-04-01 |
EP2839029A1 (en) | 2015-02-25 |
WO2013158982A1 (en) | 2013-10-24 |
RU2014146286A (en) | 2016-06-10 |
US10030262B2 (en) | 2018-07-24 |
US20150099644A1 (en) | 2015-04-09 |
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