IN2014DN09784A - - Google Patents

Info

Publication number
IN2014DN09784A
IN2014DN09784A IN9784DEN2014A IN2014DN09784A IN 2014DN09784 A IN2014DN09784 A IN 2014DN09784A IN 9784DEN2014 A IN9784DEN2014 A IN 9784DEN2014A IN 2014DN09784 A IN2014DN09784 A IN 2014DN09784A
Authority
IN
India
Prior art keywords
nucleic acid
target nucleic
sample
sample volumes
fractions
Prior art date
Application number
Inventor
Caifu Chen
Casey R Mcfarland
Jr David N Keys
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2013/032598 external-priority patent/WO2013158313A1/en
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of IN2014DN09784A publication Critical patent/IN2014DN09784A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present , counting the number of fractions with an amplified target nucleic acid , and determining the quantity of target nucleic acid in the sample.
IN9784DEN2014 2012-04-19 2014-11-19 IN2014DN09784A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261635584P 2012-04-19 2012-04-19
PCT/US2013/032598 WO2013158313A1 (en) 2012-04-19 2013-03-15 Nucleic acid amplification
PCT/US2013/037352 WO2013158982A1 (en) 2012-04-19 2013-04-19 Method of performing digital pcr

Publications (1)

Publication Number Publication Date
IN2014DN09784A true IN2014DN09784A (en) 2015-07-31

Family

ID=49384101

Family Applications (1)

Application Number Title Priority Date Filing Date
IN9784DEN2014 IN2014DN09784A (en) 2012-04-19 2014-11-19

Country Status (8)

Country Link
US (2) US10030262B2 (en)
EP (1) EP2839029B1 (en)
KR (1) KR20150011359A (en)
CN (1) CN104487592B (en)
AU (1) AU2013249123A1 (en)
IN (1) IN2014DN09784A (en)
RU (1) RU2014146286A (en)
WO (1) WO2013158982A1 (en)

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CN116497097A (en) * 2015-09-29 2023-07-28 生命技术公司 System and method for performing digital PCR
CN108779423B (en) * 2016-03-28 2021-12-21 富士胶片株式会社 Containers for PCR
WO2017196676A1 (en) * 2016-05-10 2017-11-16 Life Technologies Corporation Metal chelation post incorporation detection methods
EP3299471B1 (en) * 2016-09-23 2019-10-23 Roche Diagniostics GmbH Methods for determining the amount of a nucleic acid of interest in an unprocessed sample
EP3555314B1 (en) 2016-12-19 2023-08-16 Quantum-Si Incorporated Loading molecules into sample wells for analysis
CN106754312B (en) * 2017-01-03 2025-01-28 京东方科技集团股份有限公司 Gene sequencing chip, gene sequencing device and gene sequencing method
CN106497774A (en) * 2017-01-03 2017-03-15 京东方科技集团股份有限公司 Gene sequencing chip, gene sequencing equipment and gene order surveying method
US20180318839A1 (en) * 2017-05-03 2018-11-08 Lifeos Genomics Corporation Incubation Device Having Rotary Mechanism
US11224878B2 (en) 2017-05-05 2022-01-18 Quantum-Si Incorporated Substrates having modified surface reactivity and antifouling properties in biological reactions
JP6921638B2 (en) 2017-06-16 2021-08-18 株式会社東芝 Nucleic acid detection and quantification method, chip, assay kit, nucleic acid detection and quantification device and program
CN109504745A (en) * 2017-09-12 2019-03-22 莱弗斯基因股份有限公司 Analyze multiple target nucleic acid detection method in sample
CN107385103B (en) * 2017-09-18 2023-04-07 星源智（珠海）生物科技有限公司 Method and device for amplifying unpurified nucleic acid
CN110819523B (en) 2018-08-13 2023-08-29 上海新微技术研发中心有限公司 Digital PCR system and digital PCR liquid drop forming method
CN110819522B (en) * 2018-08-13 2023-09-22 上海新微技术研发中心有限公司 A digital PCR system and digital PCR droplet formation method
CN111349555B (en) * 2018-12-21 2023-07-18 成都万众壹芯生物科技有限公司 Digital PCR amplification device based on micropore array chip and use method thereof
WO2020167656A1 (en) 2019-02-11 2020-08-20 Ultima Genomics, Inc. Methods for nucleic acid analysis
CN112239719A (en) * 2019-07-19 2021-01-19 成都万众壹芯生物科技有限公司 Digital PCR amplification device based on micropore array chip and method for amplifying by using same
JP2022551523A (en) 2019-10-11 2022-12-09 クアンタム－エスアイ インコーポレイテッド Surface modification in gas phase
EP3990661A4 (en) * 2019-12-04 2022-07-27 Hewlett-Packard Development Company, L.P. SORTING A DROPLET WITH A BIOLOGICAL SAMPLE
KR102395365B1 (en) * 2020-02-10 2022-05-10 한국과학기술연구원 Reaction chip for PCR comprising multiple hydrogel microparticles arranged on a hydrophobic plate
CN111500408B (en) * 2020-04-24 2022-03-22 浙江大学 Kit, device and analysis method for nucleic acid analysis under fully enclosed conditions
US12296344B2 (en) 2020-11-23 2025-05-13 Frank Leo Spangler Transparent heating elements for polymerase chain reaction devices
WO2022259182A1 (en) 2021-06-09 2022-12-15 Ecole Polytechnique Federale De Lausanne (Epfl) Device to carry out semiconductor-based diagnostics
KR102537348B1 (en) * 2022-08-04 2023-05-30 한국기계연구원 Digital-PCR Analysis Substrate and Fabrication Method Thereof
KR102819404B1 (en) * 2022-08-31 2025-06-13 압티지엠제이 주식회사 Heat generating device for pcr and method thereof

Family Cites Families (17)

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US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
ES2425354T3 (en) 2004-06-01 2013-10-14 Alere San Diego, Inc. Amplification by recombinase polymerase
EP2021503A1 (en) 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
AU2007334393A1 (en) * 2006-12-14 2008-06-26 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
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US20090053719A1 (en) * 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
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WO2009067632A1 (en) 2007-11-20 2009-05-28 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2011106368A2 (en) 2010-02-23 2011-09-01 Illumina, Inc. Amplification methods to minimise sequence specific bias
WO2011106629A2 (en) 2010-02-26 2011-09-01 Life Technologies Corporation Modified proteins and methods of making and using same
EP2617061B1 (en) 2010-09-15 2021-06-30 Life Technologies Corporation Methods and apparatus for measuring analytes
CN118086471A (en) 2010-12-17 2024-05-28 生命技术公司 Methods, compositions, systems, instruments and kits for nucleic acid amplification
DE102011054101A1 (en) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Method for the spatial arrangement of sample fragments for amplification and immobilization for further derivatizations

Also Published As

Publication number Publication date
US20150099644A1 (en) 2015-04-09
EP2839029A1 (en) 2015-02-25
CN104487592B (en) 2018-08-28
CN104487592A (en) 2015-04-01
EP2839029B1 (en) 2017-11-15
US20180327813A1 (en) 2018-11-15
US10030262B2 (en) 2018-07-24
WO2013158982A1 (en) 2013-10-24
AU2013249123A1 (en) 2014-12-11
RU2014146286A (en) 2016-06-10
KR20150011359A (en) 2015-01-30

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