CN104487592B - 进行数字pcr的方法 - Google Patents

进行数字pcr的方法 Download PDF

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Publication number
CN104487592B
CN104487592B CN201380028515.6A CN201380028515A CN104487592B CN 104487592 B CN104487592 B CN 104487592B CN 201380028515 A CN201380028515 A CN 201380028515A CN 104487592 B CN104487592 B CN 104487592B
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China
Prior art keywords
sample
nucleic acid
target nucleic
amplification
ion concentration
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Expired - Fee Related
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CN201380028515.6A
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English (en)
Chinese (zh)
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CN104487592A (zh
Inventor
陈才夫
凯西·R·麦克法兰德
小大卫·N·肯斯
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Life Technologies Inc
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Life Technologies Inc
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Priority claimed from PCT/US2013/032598 external-priority patent/WO2013158313A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201380028515.6A 2012-04-19 2013-04-19 进行数字pcr的方法 Expired - Fee Related CN104487592B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261635584P 2012-04-19 2012-04-19
US61/635,584 2012-04-19
PCT/US2013/032598 WO2013158313A1 (en) 2012-04-19 2013-03-15 Nucleic acid amplification
USPCT/US2013/032598 2013-03-15
PCT/US2013/037352 WO2013158982A1 (en) 2012-04-19 2013-04-19 Method of performing digital pcr

Publications (2)

Publication Number Publication Date
CN104487592A CN104487592A (zh) 2015-04-01
CN104487592B true CN104487592B (zh) 2018-08-28

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Family Applications (1)

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CN201380028515.6A Expired - Fee Related CN104487592B (zh) 2012-04-19 2013-04-19 进行数字pcr的方法

Country Status (8)

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US (2) US10030262B2 (https=)
EP (1) EP2839029B1 (https=)
KR (1) KR20150011359A (https=)
CN (1) CN104487592B (https=)
AU (1) AU2013249123A1 (https=)
IN (1) IN2014DN09784A (https=)
RU (1) RU2014146286A (https=)
WO (1) WO2013158982A1 (https=)

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CN108779423B (zh) * 2016-03-28 2021-12-21 富士胶片株式会社 Pcr用容器
WO2017196676A1 (en) * 2016-05-10 2017-11-16 Life Technologies Corporation Metal chelation post incorporation detection methods
EP3299471B1 (en) * 2016-09-23 2019-10-23 Roche Diagniostics GmbH Methods for determining the amount of a nucleic acid of interest in an unprocessed sample
TW201837184A (zh) 2016-12-19 2018-10-16 美商寬騰矽公司 分子載入樣品孔以供分析
CN106497774A (zh) * 2017-01-03 2017-03-15 京东方科技集团股份有限公司 基因测序芯片、基因测序设备及基因测序方法
CN106754312B (zh) * 2017-01-03 2025-01-28 京东方科技集团股份有限公司 基因测序芯片、基因测序设备及基因测序方法
US20180318839A1 (en) 2017-05-03 2018-11-08 Lifeos Genomics Corporation Incubation Device Having Rotary Mechanism
JP7356909B2 (ja) 2017-05-05 2023-10-05 クアンタム－エスアイ インコーポレイテッド 生物学的反応における表面反応性と防汚特性が改変された基板
JP6921638B2 (ja) 2017-06-16 2021-08-18 株式会社東芝 核酸検出定量方法、チップ、アッセイキット、核酸検出定量装置及びプログラム
CN109504745A (zh) * 2017-09-12 2019-03-22 莱弗斯基因股份有限公司 分析样品中多重目标核酸检测方法
CN107385103B (zh) * 2017-09-18 2023-04-07 星源智（珠海）生物科技有限公司 一种非纯化核酸扩增方法及装置
CN110819522B (zh) 2018-08-13 2023-09-22 上海新微技术研发中心有限公司 一种数字pcr系统及数字pcr液滴形成方法
CN110819523B (zh) * 2018-08-13 2023-08-29 上海新微技术研发中心有限公司 一种数字pcr系统及数字pcr液滴形成方法
CN111349555B (zh) * 2018-12-21 2023-07-18 成都万众壹芯生物科技有限公司 一种基于微孔阵列芯片的数字pcr扩增装置及其使用方法
AU2020222888B2 (en) 2019-02-11 2024-08-01 Ultima Genomics, Inc. Methods for nucleic acid analysis
CN112239719A (zh) * 2019-07-19 2021-01-19 成都万众壹芯生物科技有限公司 基于微孔阵列芯片的数字pcr扩增装置和利用其进行扩增的方法
KR20220079943A (ko) 2019-10-11 2022-06-14 퀀텀-에스아이 인코포레이티드 증기 상에서의 표면 개질
WO2021112841A1 (en) 2019-12-04 2021-06-10 Hewlett-Packard Development Company, L.P. Sorting a droplet including a biologic sample
KR102395365B1 (ko) * 2020-02-10 2022-05-10 한국과학기술연구원 소수성 플레이트 상에 배열된 복수의 하이드로젤 미세입자를 포함하는 pcr용 반응 칩
CN111500408B (zh) * 2020-04-24 2022-03-22 浙江大学 在全封闭条件下进行核酸分析的试剂盒、装置及分析方法
CN115667490A (zh) * 2020-05-26 2023-01-31 生物辐射实验室股份有限公司 用于快速数字检测的装置和方法
US12296344B2 (en) 2020-11-23 2025-05-13 Frank Leo Spangler Transparent heating elements for polymerase chain reaction devices
WO2022259182A1 (en) 2021-06-09 2022-12-15 Ecole Polytechnique Federale De Lausanne (Epfl) Device to carry out semiconductor-based diagnostics
KR102595551B1 (ko) * 2021-06-23 2023-10-30 (주)옵토레인 다중 친수성을 갖는 체외진단칩
KR102537348B1 (ko) * 2022-08-04 2023-05-30 한국기계연구원 디지털 pcr 분석용 기판 및 이의 제작방법
KR102819404B1 (ko) * 2022-08-31 2025-06-13 압티지엠제이 주식회사 Pcr을 위한 열 발생 장치 및 그 방법
WO2025230778A2 (en) * 2024-04-30 2025-11-06 Gram Medical Inc. Method for processing biological samples

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Also Published As

Publication number Publication date
WO2013158982A1 (en) 2013-10-24
US20150099644A1 (en) 2015-04-09
KR20150011359A (ko) 2015-01-30
US20180327813A1 (en) 2018-11-15
EP2839029A1 (en) 2015-02-25
IN2014DN09784A (https=) 2015-07-31
RU2014146286A (ru) 2016-06-10
EP2839029B1 (en) 2017-11-15
US10030262B2 (en) 2018-07-24
CN104487592A (zh) 2015-04-01
AU2013249123A1 (en) 2014-12-11

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Granted publication date: 20180828