RU2012125624A - Варианты бета-глюкозидазы 1 с улучшенными свойствами - Google Patents
Варианты бета-глюкозидазы 1 с улучшенными свойствами Download PDFInfo
- Publication number
- RU2012125624A RU2012125624A RU2012125624/10A RU2012125624A RU2012125624A RU 2012125624 A RU2012125624 A RU 2012125624A RU 2012125624/10 A RU2012125624/10 A RU 2012125624/10A RU 2012125624 A RU2012125624 A RU 2012125624A RU 2012125624 A RU2012125624 A RU 2012125624A
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- Prior art keywords
- bgl1
- variant
- wild
- compared
- type
- Prior art date
Links
- 101710099628 Beta-glucosidase 1 Proteins 0.000 title claims abstract 319
- 230000000694 effects Effects 0.000 claims abstract 120
- 230000007062 hydrolysis Effects 0.000 claims abstract 9
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract 9
- 102000006995 beta-Glucosidase Human genes 0.000 claims abstract 8
- 108010047754 beta-Glucosidase Proteins 0.000 claims abstract 8
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract 4
- 239000008103 glucose Substances 0.000 claims abstract 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract 3
- 229910021529 ammonia Inorganic materials 0.000 claims abstract 3
- 239000001913 cellulose Substances 0.000 claims abstract 3
- 229920002678 cellulose Polymers 0.000 claims abstract 3
- 235000005822 corn Nutrition 0.000 claims abstract 3
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract 3
- 102000004169 proteins and genes Human genes 0.000 claims abstract 3
- 108090000623 proteins and genes Proteins 0.000 claims abstract 3
- 239000010902 straw Substances 0.000 claims abstract 3
- 238000006467 substitution reaction Methods 0.000 claims 26
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 239000002028 Biomass Substances 0.000 claims 2
- 101100373011 Drosophila melanogaster wapl gene Proteins 0.000 claims 2
- 241000209149 Zea Species 0.000 claims 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 208000012396 long COVID-19 Diseases 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 210000004483 pasc Anatomy 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 235000000346 sugar Nutrition 0.000 claims 1
- 150000008163 sugars Chemical class 0.000 claims 1
- 240000008042 Zea mays Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26324009P | 2009-11-20 | 2009-11-20 | |
| US61/263,240 | 2009-11-20 | ||
| PCT/US2010/057531 WO2011063308A2 (en) | 2009-11-20 | 2010-11-19 | Beta-glucosidase i variants with improved properties |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2012125624A true RU2012125624A (ru) | 2013-12-27 |
Family
ID=43608030
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012125624/10A RU2012125624A (ru) | 2009-11-20 | 2010-11-19 | Варианты бета-глюкозидазы 1 с улучшенными свойствами |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US9447400B2 (OSRAM) |
| EP (6) | EP2501806B1 (OSRAM) |
| JP (2) | JP2013511280A (OSRAM) |
| CN (2) | CN102844429B (OSRAM) |
| BR (1) | BR112012011711A2 (OSRAM) |
| CA (1) | CA2780767A1 (OSRAM) |
| DK (6) | DK2501806T3 (OSRAM) |
| IN (1) | IN2012DN03825A (OSRAM) |
| RU (1) | RU2012125624A (OSRAM) |
| WO (1) | WO2011063308A2 (OSRAM) |
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| JP7051491B2 (ja) * | 2018-02-26 | 2022-04-11 | 花王株式会社 | 変異β－グルコシダーゼ |
| US11525151B2 (en) | 2018-03-09 | 2022-12-13 | Danisco Us Inc. | Glucoamylases and methods of use, thereof |
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| JP7655849B2 (ja) | 2018-08-20 | 2025-04-02 | ウィスコンシン アルムニ リサーチ ファンデイション | ヘマグルチニン（ｈａ）タンパク質内の非ドミナントエピトープに対する免疫応答を誘起するためのベクター |
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| CN116426506B (zh) * | 2023-03-10 | 2024-01-30 | 云南师范大学 | 低温活性提高的β-木糖苷酶突变体D259G及其应用 |
| CN118006590B (zh) * | 2024-02-23 | 2024-12-27 | 南京师范大学常州合成生物学产业研究院有限公司 | 一种Microbacterium dextranolyticumβ-葡萄糖苷酶的突变体及方法和应用 |
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| FR2935986B1 (fr) | 2008-09-12 | 2012-11-23 | Inst Francais Du Petrole | Variants de beta-glucosidase a activite amelioree et leurs utilisations. |
| CA2763836A1 (en) * | 2009-05-29 | 2010-12-02 | Iogen Energy Corporation | Novel beta-glucosidase enzymes |
| WO2012125937A2 (en) | 2011-03-17 | 2012-09-20 | Danisco Us Inc. | Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis |
| DK2686434T3 (da) | 2011-03-17 | 2021-10-11 | Danisco Us Inc | Fremgangsmåde til reduktion af viskositet i saccharificeringsproces |
| US8468683B2 (en) * | 2011-10-31 | 2013-06-25 | HGST Netherlands B.V. | High bevel angle magnetic writer pole fabrication process |
| BR112015009820A2 (pt) | 2012-10-31 | 2017-08-22 | Danisco Us Inc | Composição compreendendo um polipeptídeo recombinante, ácido nucleico codi-ficando o mesmo, célula hospedeira, bem como métodos para produzir uma beta-glucosidase e para hidrolisar um substrato de biomassa lignocelulósica |
| CN104781399A (zh) | 2012-10-31 | 2015-07-15 | 丹尼斯科美国公司 | 来自稻瘟病菌的β-葡糖苷酶 |
| BR112015009201A2 (pt) | 2012-10-31 | 2017-11-14 | Danisco Us Inc | composição compreendeendo um polipeptídeo recombinante, ácido nucleico codificando o mesmo, célula hospedeira, bem como métodos para produzir uma beta-glucosidase e para hidrolisar um substrato de biomassa lignocelulósica |
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2010
- 2010-11-19 DK DK10782511.9T patent/DK2501806T3/da active
- 2010-11-19 JP JP2012540120A patent/JP2013511280A/ja active Pending
- 2010-11-19 DK DK12199330.7T patent/DK2599863T3/da active
- 2010-11-19 EP EP10782511.9A patent/EP2501806B1/en not_active Not-in-force
- 2010-11-19 EP EP12199328.1A patent/EP2602317B1/en not_active Not-in-force
- 2010-11-19 DK DK12199329.9T patent/DK2599862T3/da active
- 2010-11-19 US US13/510,902 patent/US9447400B2/en not_active Expired - Fee Related
- 2010-11-19 RU RU2012125624/10A patent/RU2012125624A/ru not_active Application Discontinuation
- 2010-11-19 EP EP12199329.9A patent/EP2599862B1/en not_active Not-in-force
- 2010-11-19 CN CN201080061975.5A patent/CN102844429B/zh not_active Expired - Fee Related
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- 2010-11-19 EP EP12199330.7A patent/EP2599863B1/en not_active Not-in-force
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- 2010-11-19 CN CN201510672711.8A patent/CN105316303B/zh not_active Expired - Fee Related
- 2010-11-19 DK DK12199328.1T patent/DK2602317T3/da active
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- 2010-11-19 CA CA2780767A patent/CA2780767A1/en not_active Abandoned
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2015
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