RU2002126969A - METHOD FOR DETERMINING Adequacy of Sample Volume in Biosensor Devices - Google Patents

METHOD FOR DETERMINING Adequacy of Sample Volume in Biosensor Devices

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Publication number
RU2002126969A
RU2002126969A RU2002126969/14A RU2002126969A RU2002126969A RU 2002126969 A RU2002126969 A RU 2002126969A RU 2002126969/14 A RU2002126969/14 A RU 2002126969/14A RU 2002126969 A RU2002126969 A RU 2002126969A RU 2002126969 A RU2002126969 A RU 2002126969A
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RU
Russia
Prior art keywords
volume
sample
biological sample
biosensor
current
Prior art date
Application number
RU2002126969/14A
Other languages
Russian (ru)
Other versions
RU2292841C2 (en
Inventor
Махиар З. КЕРМАНИ
Мария ТЕОДОРЧИК
Шерри Кс. ГУО
Original Assignee
Лайфскен, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from US09/974,597 external-priority patent/US6797150B2/en
Application filed by Лайфскен, Инк. filed Critical Лайфскен, Инк.
Publication of RU2002126969A publication Critical patent/RU2002126969A/en
Application granted granted Critical
Publication of RU2292841C2 publication Critical patent/RU2292841C2/en

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Claims (16)

1. Способ определения объема биологической пробы, удерживаемой внутри электрохимического биодатчика, заключающийся в том, что прикладывают к биодатчику напряжение переменного тока, имеющее выбранную амплитуду и выбранную частоту, измеряют ток, генерируемый за счет приложения напряжения переменного тока, определяют емкость биодатчика в соответствии с измеренным током, определяют площадь поверхности биодатчика, находящуюся в контакте с пробой, на основе упомянутой измеренной емкости, и определяют объем пробы внутри биодатчика по измеренной площади поверхности.1. A method for determining the volume of a biological sample held inside an electrochemical biosensor, which consists in applying an alternating current voltage having a selected amplitude and a selected frequency to the biosensor, measuring the current generated by applying an alternating current voltage, determining the biosensor capacitance in accordance with the measured current, determine the surface area of the biosensor in contact with the sample based on the measured capacitance, and determine the volume of the sample inside the biosensor by constant surface area. 2. Способ по п.1, отличающийся тем, что дополнительно определяют, является ли объем пробы адекватным для проведения измерения одной или более выбранных характеристик пробы.2. The method according to claim 1, characterized in that it further determines whether the sample volume is adequate to measure one or more selected characteristics of the sample. 3. Способ по п.2, отличающийся тем, что дополнительно измеряют концентрацию одного или нескольких исследуемых веществ, присутствующих в пробе, причем измерение проводят, если упомянутый объем пробы является адекватным.3. The method according to claim 2, characterized in that it further measures the concentration of one or more of the test substances present in the sample, and the measurement is carried out if the said sample volume is adequate. 4. Способ по п.2, отличающийся тем, что дополнительно определяют необходимый коэффициент компенсации, предназначенный для компенсации неадекватного объема на основе определения, показавшего, что объем пробы является неадекватным, осуществляют компенсацию неадекватного объема пробы.4. The method according to claim 2, characterized in that it further determines the necessary compensation coefficient, designed to compensate for the inadequate volume on the basis of a determination that showed that the sample volume is inadequate, compensate for the inadequate sample volume. 5. Способ по п.4, отличающийся тем, что для определения необходимого коэффициента компенсации определяют отношение емкости биодатчика, когда он полностью заполнен пробой, к емкости биодатчика с неадекватным объемом пробы.5. The method according to claim 4, characterized in that to determine the required compensation coefficient, the ratio of the capacity of the biosensor when it is completely filled with the sample to the capacity of the biosensor with an inadequate sample volume is determined. 6. Способ по п.1, отличающийся тем, что дополнительно определяют сопротивления биодатчика на основе измеренного тока.6. The method according to claim 1, characterized in that it further determines the resistance of the biosensor based on the measured current. 7. Способ по п.6, отличающийся тем, что дополнительно определяют площадь поверхности биодатчика, находящейся в контакте с пробой, на основе емкости и сопротивления.7. The method according to claim 6, characterized in that it further determines the surface area of the biosensor in contact with the sample based on the capacitance and resistance. 8. Способ по п.1, отличающийся тем, что дополнительно прикладывают напряжение постоянного тока к биодатчику.8. The method according to claim 1, characterized in that it further applies a DC voltage to the biosensor. 9. Способ по п.8, отличающийся тем, что приложение напряжения постоянного тока и напряжения переменного тока осуществляют одновременно.9. The method according to claim 8, characterized in that the application of DC voltage and AC voltage is carried out simultaneously. 10. Способ компенсации неадекватного объема биологической пробы, удерживаемой внутри электрохимического биодатчика для измерения по меньшей мере одной характеристики биологической пробы, заключающийся в том, что определяют отношение емкости биодатчика, когда он полностью заполнен пробой, к емкости биодатчика с неадекватным объемом пробы.10. A method of compensating for an inadequate volume of a biological sample held inside an electrochemical biosensor for measuring at least one characteristic of a biological sample, which consists in determining the ratio of the capacity of the biosensor when it is completely filled with the sample to the capacity of the biosensor with an inadequate sample volume. 11. Способ измерения по меньшей мере одной характеристики биологической пробы, удерживаемой внутри электрохимического биодатчика, заключающийся в том, что определяют объем биологической пробы, определяют адекватность объема биологической пробы, компенсируют неадекватный объем биологической пробы, удерживаемой внутри электрохимического биодатчика, на основе определения, показавшего, что объем является неадекватным, точно измеряют по меньшей мере одну характеристику биологической пробы.11. A method for measuring at least one characteristic of a biological sample held inside an electrochemical biosensor, which consists in determining the volume of the biological sample, determining the adequacy of the volume of the biological sample, compensating for the inadequate volume of the biological sample held inside the electrochemical biosensor, based on a determination that showed that the volume is inadequate, at least one characteristic of the biological sample is accurately measured. 12. Способ по п.11, отличающийся тем, что по меньшей мере одна характеристика биологической пробы является концентрацией по меньшей мере одного исследуемого вещества в пробе.12. The method according to claim 11, characterized in that at least one characteristic of the biological sample is the concentration of at least one analyte in the sample. 13. Система определения объема биологической пробы внутри химического источника тока, имеющего площадь поверхности и объем, содержащая источник напряжения, обеспечивающий приложение напряжения к химическому источнику тока, средство для измерения тока, генерируемого источником тока, когда к этому источнику тока приложено напряжение, средство для определения емкости источника тока на основе измеренного тока, средство для определения площади поверхности источника тока, покрытой биологической пробой, на основе емкости источника тока, и средство для определения объема биологической пробы, исходя из площади поверхности источника тока.13. A system for determining the volume of a biological sample inside a chemical current source having a surface area and a volume containing a voltage source that provides voltage to the chemical current source, means for measuring the current generated by the current source when voltage is applied to this current source, means for determining the capacity of the current source based on the measured current, means for determining the surface area of the current source coated with a biological sample, based on the capacity of the current source, and COROLLARY for determining the volume of a biological sample, based on the area of the current source surface. 14. Система по п.13, отличающаяся тем, что дополнительно содержит средство для определения того, является ли объем пробы адекватным для проведения точного измерения концентрации одного или более выбранных исследуемых веществ в биологической пробе.14. The system according to item 13, characterized in that it further comprises a means for determining whether the sample volume is adequate to accurately measure the concentration of one or more selected test substances in a biological sample. 15. Система по п.14, отличающаяся тем, что дополнительно содержит средство для измерения концентрации одного или более выбранных исследуемых веществ в биологической пробе и средство для компенсации объема пробы, определенного как неадекватный при измерении концентрации одного или более выбранных исследуемых веществ в биологической пробе.15. The system according to 14, characterized in that it further comprises a means for measuring the concentration of one or more selected test substances in a biological sample and a means for compensating for the volume of a sample determined to be inadequate when measuring the concentration of one or more selected test substances in a biological sample. 16. Система для использования совместно с измерительным прибором, обеспечивающим контакт с химическим источником тока и измерение концентрации одного или более выбранных исследуемых веществ в биологической пробе, удерживаемой в химическом источнике тока, содержащая источник напряжения, обеспечивающий приложение напряжения переменного и/или постоянного тока к химическому источнику тока, и электронную схему, обеспечивающую прием тока, генерируемого химическим источником тока, когда к нему приложено напряжение, измерение упомянутого генерируемого тока, определение емкости химического источника тока на основе измеренного тока, определение площади поверхности химического источника тока, покрытой биологической пробой, на основе емкости источника тока, и определение объема биологической пробы в соответствии с площадью поверхности источника тока, покрытой биологической пробой.16. A system for use in conjunction with a measuring device that provides contact with a chemical current source and measures the concentration of one or more selected test substances in a biological sample held in a chemical current source, containing a voltage source that provides an alternating and / or direct current voltage to the chemical a current source, and an electronic circuit that receives current generated by a chemical current source when voltage is applied to it, measuring said neriruemogo current determination of the chemical source based on the detected current of the current capacity, the definition area of the electrochemical surface coated biological sample, based on power source capacity, and determining the volume of a biological sample in accordance with the surface area of the current source, the coated biological sample.
RU2002126969/14A 2001-10-10 2002-10-09 Metod of determining adequacy of sample's volume in biological detector devices RU2292841C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/974,597 US6797150B2 (en) 2001-10-10 2001-10-10 Determination of sample volume adequacy in biosensor devices
US09/974,597 2001-10-10

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RU2002126969A true RU2002126969A (en) 2004-04-10
RU2292841C2 RU2292841C2 (en) 2007-02-10

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US (2) US6797150B2 (en)
JP (1) JP4354168B2 (en)
KR (1) KR100968354B1 (en)
CN (3) CN101311723B (en)
CA (2) CA2407249C (en)
CZ (1) CZ20023368A3 (en)
HK (1) HK1055463A1 (en)
IL (1) IL152214A0 (en)
MX (1) MXPA02010106A (en)
PL (1) PL208126B1 (en)
RU (1) RU2292841C2 (en)
TW (1) TW581866B (en)

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