RU1811864C - Method for therapy of cow mastitis due to conditionality pathogeneous microflora - Google Patents

Abstract

Usage: in veterinary medicine. SUMMARY OF THE INVENTION: a method for treating mastitis in cows by intracisternally administering staphylococcal, sevdomonas, protea and colibacteriophage and T-activin, the phages being mixed in equal parts and the T-activin being administered subcutaneously, the administration being carried out over 2- 3 days 6 tab.

Description

The invention relates to veterinary medicine, in particular to the treatment of mastitis in cows.

The purpose of the invention is to increase the effectiveness of treatment by increasing the specific effect on the conditionally pathogenic microflora and increasing non-specific factors of the body's resistance.

To achieve this goal in the treatment of cows for mastitis caused by conditionally pathogenic microflora using intracisternal administration of staphylococcal bacteriophage, pseudomonas, proteus and colibacteriophages and T-activin are additionally introduced, moreover, the staphylococcal bacteriophage is mixed in equal parts with pseudomonas -bacteriophages, and the mixture is administered at a dose of 45-50 ml per share of the udder 2 times a day at intervals of 6-7 hours for 2-3 days, with the introduction of T-activin is carried out subcutaneously in a dose of 0,19-0,21 ml per 1 kg body weight one time daily for. 2-3 days.

The method is carried out as follows. The following bacteriophages are mixed in equal proportions: staphylococcal, pseudomonas, Proteus and coli. The obtained polybacteriophage is administered intracisternally to sick cows, 45-50 ml per each udder share 2 times a day with an interval of hours for 2-3 days,

Example. At a dairy farm with a livestock of 240 cows, 72 cows fell ill with mastitis caused by opportunistic microflora. For the treatment of diseased animals, the proposed method was used, according to which the following bacteriophages were pre-mixed: staphylococcal, pseudomonas, protein and coli. The resulting polybacteriophage, consisting of equal amounts of these monophages, was administered intracisternally to sick cows at a dose of 50 ml per one udder 2 times a day with an interval of 7 hours for 3 days. At the same time, T-activin was administered subcutaneously at a dose of 0.2 ml per 1 kg of live weight once a day

with

00

about

N

also for 3 days. As a result of the treatment, 68 cows recovered.

To determine the optimal parameters of the method, a series of experiments were carried out, the results of which are given in Table. 1-6.

As follows from the table. 1, the introduction of polybacteriophage in a dose of less than 45 ml is ineffective or ineffective. With a dose increase of more than 50 ml, further treatment efficacy does not occur. Thus, a single dose of intracisternally administered polybacteriophage in the range of 45-50 ml per one udder is optimal.

Thus, with the introduction of the polybacteriophage mee. 4 times the treatment efficiency is not achieved. The introduction of the polybacteriophage within 2-3 days, i.e. 4-6 times, is optimal. Further administration of polybacteriophage does not lead to an increase in liver efficiency.

The optimal dose of T-activin is 0.19-0.21 ml per 1 kg of live weight. A smaller amount of the drug does not provide a sufficiently high treatment efficiency, and an increase in the dose does not lead to an increase in the recovery rate of diseased animals.

As follows from the table. 4, T-activin is advisable to enter 1 time per day for 2-3 days. A single administration of it does not provide a sufficiently high treatment efficiency. With continued administration of T-activin for more than 3 days, further treatment efficacy does not increase.

Analysis data table. 5 indicates Q that the greatest degree of in vitro lysis of microorganism cultures is achieved by contact with a polybacteriophage within 6-7 hours. Therefore, the optimal interval between two administrations of the polybacteriophage is 6-7 hours.

Thus, as follows from the above studies, the optimal therapeutic efficacy of treating mastitis caused by conditionally pathogenic microflora is achieved by subcutaneous administration of T-activin to cows at a dose of 0.190, 21 ml per 1 kg of live weight once a day for 2-3 days, in combination with intracisternal administration of mixed in equal proportions staphylococcal, pseudomonas, Proteus and coli bacteriophages, at a dose of 45-50 ml per one udder 2 times a day with an interval of 6-7 hours also for 2-3 days, Such exercise both treatments for mastitis caused by conditionally pathogenic microflora,

provides therapeutic efficacy of about 96.7%.

Claims (1)

SUMMARY OF THE INVENTION A method for treating mastitis in cows caused by conditionally pathogenic microflora, comprising intracisternal administration of a staphylococcal bacteriophage, characterized in that, in order to increase the effectiveness of the method, pseudomonas, proteic and colic bacteriophages and T-activin are additionally introduced, moreover, the staphylococcal bacteriophage is mixed before administration in equal parts with pseudomonas, proteus and coli bacteriophages, and the mixture is administered in a dose of 45-50 ml per share of the udder 2 times a day with an interval of 6-7 hours for 2-3 days, T-activin administering is performed subcutaneously at a dose of 0,19-0,21 ml per 1 kg of live weight 1 times a day for 2-3 days. Determination of a single dose of polybacteriophage Table 1 Determination of the frequency of administration of polybacteriophage .Table 1 -. ... Determination of the optimal dose of T-activin in combination with: polybacteriophage Determination of the frequency of administration of T-activin Determination of g vitro degree of lysis by a polybacteriophage of conditionally pathogenic L. microflora causing mastitis in cows table 2 That blitz 4 Table 5 Table b Comparative therapeutic efficacy of drugs