RU2185838C2 - Method for prophylaxis of colibacteriosis in calves - Google Patents

Abstract

FIELD: veterinary science. SUBSTANCE: calves are fed with lactobacterin thrice daily for 4 d per 20 billion cells mixed with colostrum one liter of which is additionally supplemented with 0.03 g manganous tetrachloride, 0.5 g fructose and 0.5 g lactose. Prophylactic efficiency of bacterial preparation is increased and intestinal adaptability of lacto- and bifidobacteria is improved in calves. EFFECT: improved prophylaxis of colibacteriosis. 2 tbl

Description

 The invention relates to veterinary medicine and is intended to increase the efficiency of the use of lactobacterin in the prevention of calf colibacillosis.

 For the prevention of colibacillosis of calves, various probiotics are used. One of the ways to increase the effectiveness of probiotics is the development and preparation of drugs consisting of not one, two or three types of antagonist bacteria [1, 2]. In order to prevent and treat calf diarrhea, including colibacteriosis, newborns are first absorbed with adsorbents and then probiotics are used [3]. An unconventional method of administering probiotics not by mouth, but intraperitoneally is also used [4].

 One of the ways to increase the effectiveness of treatment and prophylaxis of diarrhea is the joint use of antibacterial agents and antibiotic-resistant probiotics [5] or their use together with immunomodulators [6].

 There is a method of combined use of lactobacterin T and B-activin for the correction of intestinal microbiocenosis in calf diarrhea [7]. In this method, a probiotic normalizes intestinal bacteriocenosis, and immunomodulators reduce the degree of immunodeficiency and increase the resistance of animals. But T and B-activin do not affect the survival of lactobacilli and bifidobacteria in the animal body. On the other hand, different routes of drug administration are used here (by mouth and subcutaneously), and lactobacterin is drunk before colostrum is taken, which leads to an increase in the number of operations (procedures) and additional time spent on their implementation.

 The purpose of the invention is to increase the prophylactic effectiveness of lactobacterin in colibacteriosis of calves and reduce the number of preventive operations.

 The task is achieved in that in order to increase the effectiveness of the preventive action, reduce the number of operations and the time for their carrying out, newborn calves are thrice fed 20 billion MT of lactobacterin three times a day for four days in a mixture with colostrum, 0.3 liter of which is added g of manganese tetrahydrate chloride, 0.5 g of fructose and 0.5 g of lactose, which contribute to an increase in the number of lactobacilli and bifidobacteria and their better survival in the intestines of animals.

 Example 1

 An increase in the number of lactobacilli and bifidobacteria in the intestines of calves after the combined use of lactobacterin, manganese chloride, fructose and lactose.

 The experiments were performed on 3 groups of calves with 5 goals in each. The calves of the first group served as controls. The calves of the second group were fed lactobacterin of 20 billion MT for 20-30 minutes before feeding 3 times a day for four days. The calves of the third group lactobacterin in the same dose was drunk together with colostrum, in which 0.3 g of tetrahydrate chloride manganese, 0.5 g of fructose and 0.5 g of lactose were also added 3 times a day for four days. On the 8th day, the contents of the rectum were taken from calves for the quantitative determination of lactobacilli and bifidobacteria on Bogdanov and Blaurock environments. As can be seen from table 1, the use of lactobacterin with manganese salt, fructose and lactose has significantly increased the titers of lactobacilli and bifidobacteria in the intestines of the calves.

 Examples 2

 A comparative definition of the prophylactic effectiveness of lactobacterin with immunomodulators and the proposed method (lactobacterin with fructose, lactose and manganese chloride).

 The experiment was performed on four groups of calves (10 animals each) on a farm where there was a massive incidence and departure of newborns from colibacteriosis. From the first day of birth, calves of the first group 3 times a day for 4 days together with colostrum were fed 20 billion MT of lactobacterin, 0.5 g of fructose, 0.5 g of lactose and 0.3 g of tetrahydrate manganese chloride.

 From the first day of birth, 20 billion MT of lactobacterin were fed 3 times a day to calves of the second group 20-30 minutes before feeding for 4 days. In addition, animals were administered once a day for 3 days subcutaneously with T and B-activin. The first was injected with 1 ml of 0.01% solution, the second - 6 mg in 1 ml of saline per head.

 For calves of the third group, only lactobacterin at a dose of 20 billion MT was drunk 20-30 minutes before feeding 3 times a day for 4 days.

 Calves of the fourth (control) group of prophylactic agents were not used. Among animals of all groups, morbidity, mortality, duration of the disease, and time of its occurrence (in days) were taken into account.

 A comparison of the results shows that the proposed method for the prevention of colibacillosis is more effective compared to the analogue and prototype, which is expressed by a reduction in the incidence of 10-20%, the duration of the disease by 1.4-0.5 days and its later occurrence.

 Comparative data on the effectiveness of various methods for the prevention of colibacteriosis of calves are shown in table 2.

 The proposed method prevents the occurrence of diarrhea in 60% of calves, eliminates the possibility of death among them, reduces the duration of the disease by 0.5 days compared with the analogue and the time and money spent on therapeutic and preventive measures (operations). We hope that it will find application in production.

Sources of information
1. Golikov A.V., Marchuk A.T., Nesterenko Z.I., Shiryaeva V.T. Prevention of colibacteriosis in newborn calves with lactobrevicolibacterin. Proceedings of the All-Union Institute of Experimental Veterinary Medicine. M., 1980, v. 51.

 2. Ivanovsky A.A. A new probiotic (bactocellolactin) for various pathologies in animals. Veterinary Medicine, 1996, 11.

 3. Sisyagi P.N., Panilov N.A., Zotkin G.V. A method of treating gastrointestinal diseases of calves. USSR copyright certificate 708961, 1977.

 4. Sivers B. C., Dyachenko D.P., Gnatenko G.V., Gribennikov V.I. A method for the prevention and treatment of gastrointestinal diseases of young farm animals. USSR copyright certificate 708961, 1979.

 5. Zhirkov I.N., Bratukhin I.I. The use of the probiotic RAS for the correction of dysbiosis in calves. Veterinary Medicine, 1999, 4.

 6. Panin A.N., Serykh H.I., Malik E.V., Goraev I.M., Borovikov N.V., Denisov A.A. Improving the effectiveness of probiotic therapy in piglets. Veterinary Medicine, 1996, 3.

 7. Gryaznova T. N., Pavlova I. B., Voronin E. S., Panichkov M. A. Biological means of correction of calves intestinal microbiocenosis. Veterinary Medicine, 1991, 7, pp. 23-24.

Claims (1)

 A method for preventing colibacteriosis of calves, including feeding them lactobacterin, characterized in that the calves are fed lactobacterin three times a day for four days at 20 billion m. mixed with colostrum, per liter of which is added 0.03 g of manganese chloride tetrahydrate, 0.5 g of fructose and 0.5 g of lactose.

Images