RO116208B1 - Procedeu de obţinere prin biosinteză a glucoamilazelor utilizând o tulpină selecţionată de aspergillus awamori - Google Patents
Procedeu de obţinere prin biosinteză a glucoamilazelor utilizând o tulpină selecţionată de aspergillus awamoriInfo
- Publication number
- RO116208B1 RO116208B1 RO98-00619A RO9800619A RO116208B1 RO 116208 B1 RO116208 B1 RO 116208B1 RO 9800619 A RO9800619 A RO 9800619A RO 116208 B1 RO116208 B1 RO 116208B1
- Authority
- RO
- Romania
- Prior art keywords
- glucoamylases
- biosynthesis
- preparing
- aspergillus awamori
- selected strain
- Prior art date
Links
- 241001513093 Aspergillus awamori Species 0.000 title abstract 2
- 102100022624 Glucoamylase Human genes 0.000 title abstract 2
- 108050008938 Glucoamylases Proteins 0.000 title abstract 2
- 230000015572 biosynthetic process Effects 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 240000008042 Zea mays Species 0.000 abstract 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 2
- 235000005822 corn Nutrition 0.000 abstract 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 abstract 1
- 235000019764 Soybean Meal Nutrition 0.000 abstract 1
- 238000005273 aeration Methods 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- 239000001110 calcium chloride Substances 0.000 abstract 1
- 235000011148 calcium chloride Nutrition 0.000 abstract 1
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 abstract 1
- 235000019797 dipotassium phosphate Nutrition 0.000 abstract 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 230000002779 inactivation Effects 0.000 abstract 1
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract 1
- 239000007791 liquid phase Substances 0.000 abstract 1
- 235000012054 meals Nutrition 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000011707 mineral Substances 0.000 abstract 1
- 235000010755 mineral Nutrition 0.000 abstract 1
- 230000000050 nutritive effect Effects 0.000 abstract 1
- 229910001562 pearlite Inorganic materials 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 230000000630 rising effect Effects 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 239000004455 soybean meal Substances 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Invenţia se referă la un procedeu de obţinere prin biosinteză a glucoamilazelor cu o tulpină selecţionată de Aspergillus awamori, care constă în aceea că, microorganismul se cultivă submers, în sistem discontinuu, în mediu nutritiv conţinând făină de porumb 2...3 g%, făină de soia 1...2 g%, extract de porumb 2...4 g% cu 48...50% s.u. şi supliment de săruri minerale KHPO0,1...0,5 g% şi (NH)SO0,1...0,5 g%, urmată de obţinerea preparatului enzimatic, prin filtrarea soluţiei enzimatice native pe filtrul Nutsche cu strat de perlită pe pânză petrol şi concentrarea fazei lichide rezultate, sub vacuum, la temperaturi cuprinse între 35 şi 40°C, pentru a se evita fenomenul de inactivare termică, parametrii indicaţi pentru desfăşurarea bioprocesului fiind temperatura 28...31°C,aerarea 0,1...0,3 1/1 min, iniţial, şi ridicată lent, pe parcursul fermentaţiei, până la 0,4...0,6 1/1 min, agitarea 200...300 rpm, pH-ul care rezultă după sterilizarea mediului este de 4,7...5,2, iar soluţiile enzimatice native se stabilizează prin adaos de CaCl0,1 g/%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO98-00619A RO116208B1 (ro) | 1998-02-27 | 1998-02-27 | Procedeu de obţinere prin biosinteză a glucoamilazelor utilizând o tulpină selecţionată de aspergillus awamori |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO98-00619A RO116208B1 (ro) | 1998-02-27 | 1998-02-27 | Procedeu de obţinere prin biosinteză a glucoamilazelor utilizând o tulpină selecţionată de aspergillus awamori |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RO116208B1 true RO116208B1 (ro) | 2000-11-30 |
Family
ID=64358109
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO98-00619A RO116208B1 (ro) | 1998-02-27 | 1998-02-27 | Procedeu de obţinere prin biosinteză a glucoamilazelor utilizând o tulpină selecţionată de aspergillus awamori |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO116208B1 (ro) |
-
1998
- 1998-02-27 RO RO98-00619A patent/RO116208B1/ro unknown
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