PE20110908A1 - Metodo de obtencion y produccion masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes - Google Patents
Metodo de obtencion y produccion masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantesInfo
- Publication number
- PE20110908A1 PE20110908A1 PE2011001286A PE2011001286A PE20110908A1 PE 20110908 A1 PE20110908 A1 PE 20110908A1 PE 2011001286 A PE2011001286 A PE 2011001286A PE 2011001286 A PE2011001286 A PE 2011001286A PE 20110908 A1 PE20110908 A1 PE 20110908A1
- Authority
- PE
- Peru
- Prior art keywords
- cylindrospermopsis
- na2edta
- cyanobacteria
- obtaining
- producing
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
SE REFIERE A UN METODO DE OBTENCION Y PRODUCCION DE UNA CIANOBACTERIA PRODUCTORA DE FICOTOXINAS PARALIZANTES DEL GENERO CYLINDROSPERMOPSIS RACIBORSKII, ANABAENA CIRCINALIS, ENTRE OTROS, EL CUAL COMPRENDE: A) INOCULAR ENTRE 20 Y 40 MILLONES DE FILAMENTOS DE CIANOBACTERIA POR CADA 3L DE MEDIO DE CULTIVO MLA QUE CONTIENE MgSO4 7H2O, NaNO3, K2HPO4, H3BO3, H2SeO4, BIOTINA, VITAMINA B12, TIAMINA HCl, CuSO4.5H2O, ZnSO4.7H2O, CoCl2.6H2O, NaMoO4.2H2O, Na2EDTA, FeCl3.6H2O, NaHCO3, MnCl2.H2O COMPLEMENTADO CON 2,8mM DE ARGININA, 1,7mM DE METIONINA Y 1,0mM DE ACIDO ALANTOICO; B) CULTIVAR LA CIANOBACTERIA EN EL MEDIO DE CULTIVO A UNA TEMPERATURA ENTRE 15 Y 35ºC CON LUZ NATURAL O ARTIFICIAL; C) RECOLECTAR UN VOLUMEN DE MEDIO DE CULTIVO ENTRE 20 A 40% DEL VOLUMEN TOTAL CADA 1 A 3 DIAS Y REPONER EL VOLUMEN RETIRADO CON MEDIO DE CULTIVO FRESCO; D) MANTENER EL CULTIVO EN MODO CONTINUO; E) CENTRIFUGAR EL MEDIO CON LAS CIANOBACTERIAS RECOLECTADO EN (C) ENTRE 5.000 A 15.000 X G ENTRE 5 Y 30 MINUTOS. DICHO METODO SE CARACTERIZA POR SU EFICIENCIA, ALTO RENDIMIENTO, SIMPLEZA Y ALTA RENTABILIDAD
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CL2009000722A CL2009000722A1 (es) | 2009-03-24 | 2009-03-24 | Metodo de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes, utilizando un medio de cultivo mla suplementado con arginina, metionina y ácido alantoico. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PE20110908A1 true PE20110908A1 (es) | 2012-01-19 |
Family
ID=42780211
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PE2011001286A PE20110908A1 (es) | 2009-03-24 | 2010-03-18 | Metodo de obtencion y produccion masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP2412796B1 (es) |
| JP (1) | JP5757044B2 (es) |
| KR (1) | KR101296533B1 (es) |
| AR (1) | AR076144A1 (es) |
| BR (1) | BRPI1013549B1 (es) |
| CA (1) | CA2754158C (es) |
| CL (1) | CL2009000722A1 (es) |
| DK (1) | DK2412796T3 (es) |
| ES (1) | ES2523373T3 (es) |
| HR (1) | HRP20141069T1 (es) |
| MX (1) | MX2011010019A (es) |
| PE (1) | PE20110908A1 (es) |
| PT (1) | PT2412796E (es) |
| WO (1) | WO2010109387A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8957207B2 (en) | 2009-03-24 | 2015-02-17 | Proteus S.A. | Methods for producing phycotoxins |
| PE20160011A1 (es) | 2013-03-15 | 2016-02-10 | Childrens Medical Center | Formulaciones combinadas de neosaxitoxina para una anestesia local prolongada |
| CN108949847B (zh) * | 2018-08-01 | 2021-06-18 | 浙江海洋大学 | 一种利用海洋细菌发酵制备膝沟藻毒素的方法 |
| CN112110930B (zh) * | 2020-09-16 | 2022-03-29 | 中国水产科学研究院东海水产研究所 | 一种从有毒贝壳中提取新石房蛤毒素的方法 |
-
2009
- 2009-03-24 CL CL2009000722A patent/CL2009000722A1/es unknown
-
2010
- 2010-03-18 ES ES10755519.5T patent/ES2523373T3/es active Active
- 2010-03-18 BR BRPI1013549A patent/BRPI1013549B1/pt active IP Right Grant
- 2010-03-18 WO PCT/IB2010/051188 patent/WO2010109387A1/es active Application Filing
- 2010-03-18 DK DK10755519.5T patent/DK2412796T3/en active
- 2010-03-18 MX MX2011010019A patent/MX2011010019A/es active IP Right Grant
- 2010-03-18 EP EP10755519.5A patent/EP2412796B1/en active Active
- 2010-03-18 KR KR1020117021026A patent/KR101296533B1/ko active IP Right Grant
- 2010-03-18 JP JP2012501445A patent/JP5757044B2/ja active Active
- 2010-03-18 CA CA2754158A patent/CA2754158C/en active Active
- 2010-03-18 PT PT107555195T patent/PT2412796E/pt unknown
- 2010-03-18 PE PE2011001286A patent/PE20110908A1/es active IP Right Grant
- 2010-03-23 AR ARP100100935A patent/AR076144A1/es active IP Right Grant
-
2014
- 2014-11-03 HR HRP20141069AT patent/HRP20141069T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010109387A1 (es) | 2010-09-30 |
| ES2523373T3 (es) | 2014-11-25 |
| JP5757044B2 (ja) | 2015-07-29 |
| KR101296533B1 (ko) | 2013-08-13 |
| EP2412796A1 (en) | 2012-02-01 |
| PT2412796E (pt) | 2014-11-12 |
| BRPI1013549B1 (pt) | 2018-09-25 |
| HRP20141069T1 (hr) | 2015-01-30 |
| EP2412796B1 (en) | 2014-08-06 |
| MX2011010019A (es) | 2011-10-11 |
| AR076144A1 (es) | 2011-05-18 |
| EP2412796A4 (en) | 2013-02-27 |
| CA2754158A1 (en) | 2010-09-30 |
| KR20110132368A (ko) | 2011-12-07 |
| DK2412796T3 (en) | 2014-11-17 |
| BRPI1013549A2 (pt) | 2015-08-25 |
| CA2754158C (en) | 2015-07-14 |
| JP2012521212A (ja) | 2012-09-13 |
| CL2009000722A1 (es) | 2009-06-19 |
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