HRP20141069T1 - Postupak dobivanja i proizvodnje na veliko izoliranih vrsta cijanobakterija koje proizvode paralizirajuä†e fikotoksine - Google Patents
Postupak dobivanja i proizvodnje na veliko izoliranih vrsta cijanobakterija koje proizvode paralizirajuä†e fikotoksine Download PDFInfo
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- HRP20141069T1 HRP20141069T1 HRP20141069AT HRP20141069T HRP20141069T1 HR P20141069 T1 HRP20141069 T1 HR P20141069T1 HR P20141069A T HRP20141069A T HR P20141069AT HR P20141069 T HRP20141069 T HR P20141069T HR P20141069 T1 HRP20141069 T1 HR P20141069T1
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- Prior art keywords
- culture medium
- cyanobacteria
- culture
- final
- mass production
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- 238000000034 method Methods 0.000 title claims 8
- 241000192700 Cyanobacteria Species 0.000 title claims 5
- 231100000769 Phycotoxin Toxicity 0.000 title claims 2
- 239000001963 growth medium Substances 0.000 claims 7
- NUCLJNSWZCHRKL-UHFFFAOYSA-N allantoic acid Chemical compound NC(=O)NC(C(O)=O)NC(N)=O NUCLJNSWZCHRKL-UHFFFAOYSA-N 0.000 claims 4
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 239000004475 Arginine Substances 0.000 claims 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 2
- 238000005119 centrifugation Methods 0.000 claims 2
- 229930182817 methionine Natural products 0.000 claims 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims 2
- 241000192660 Aphanizomenon Species 0.000 claims 1
- 244000085413 Aphanizomenon flos aquae Species 0.000 claims 1
- 235000013781 Aphanizomenon flos aquae Nutrition 0.000 claims 1
- 241000531070 Aphanizomenon gracile Species 0.000 claims 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims 1
- 241001299747 Cylindrospermopsis raciborskii Species 0.000 claims 1
- 241001559570 Dolichospermum circinale Species 0.000 claims 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims 1
- 229910003599 H2SeO4 Inorganic materials 0.000 claims 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims 1
- 241001181287 Microseira wollei Species 0.000 claims 1
- 229910018890 NaMoO4 Inorganic materials 0.000 claims 1
- 229930003779 Vitamin B12 Natural products 0.000 claims 1
- 229940054349 aphanizomenon flos-aquae Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims 1
- 235000019797 dipotassium phosphate Nutrition 0.000 claims 1
- 239000008187 granular material Substances 0.000 claims 1
- 238000011081 inoculation Methods 0.000 claims 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims 1
- 235000019341 magnesium sulphate Nutrition 0.000 claims 1
- 239000011565 manganese chloride Substances 0.000 claims 1
- 235000002867 manganese chloride Nutrition 0.000 claims 1
- 239000002609 medium Substances 0.000 claims 1
- QYHFIVBSNOWOCQ-UHFFFAOYSA-N selenic acid Chemical compound O[Se](O)(=O)=O QYHFIVBSNOWOCQ-UHFFFAOYSA-N 0.000 claims 1
- 235000017557 sodium bicarbonate Nutrition 0.000 claims 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims 1
- 235000010344 sodium nitrate Nutrition 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 235000019157 thiamine Nutrition 0.000 claims 1
- 239000011721 thiamine Substances 0.000 claims 1
- 239000011715 vitamin B12 Substances 0.000 claims 1
- 235000019163 vitamin B12 Nutrition 0.000 claims 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims 1
- 229910000368 zinc sulfate Inorganic materials 0.000 claims 1
- 239000011686 zinc sulphate Substances 0.000 claims 1
- 235000009529 zinc sulphate Nutrition 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Claims (6)
1. Postupak dobivanja i proizvodnje na veliko izoliranih vrsta cijanobakterija koje proizvode paralizirajuće fikotoksine, izabranih iz skupine koja obuhvaća Cylindrospermopsis raciborskii, Aphanizomenon flos-aquae, Aphanizomenon (Aph) issatschenkoi (Usacev) Proskina-Lavrenco, Anabaena circinalis, Lyngbya wollei i Aphanizomenon gracile (Lemm) Lemm, naznačeno time što taj postupak obuhvaća korake:
a. inokuliranje od 20 do 40 milijuna cijanobakterijskih filamenata na svake 3 litre MLA medija kulture koji sadrži MgSO4 · 7H2O, NaNO3, K2HPO4, H3BO3, H2SeO4, biotin, vitamin B12, tiamin HCl, CuSO4 · 5H2O, ZnSO4 · 7H2O , CoCl2 · 6H20, NaMoO4 · 2H20 , Na2EDTA, FeCl3 · 6H2O, NaHCO3, MnCl2 · H2O, s dodatkom od 2 do 3.5 mM arginina, od 1 do 2.2 mM metionina i 0.7 do 1.3 mM alantoinske kiseline;
b. uzgajanje kulture cijanobakterija u mediju kulture na temperaturi od 15 do 35°C pod stalnim prirodnim ili umjetnim osvjetljenjem;
c. uzimanje volumena medija kulture u rasponu od 20 do 40% ukupnog volumena svakih 1 do 3 dana i zamjena uklonjenog volumena svježim medijem kulture;
d. održavanje kulture u kontinuiranom stanju;
e. centrifugiranje medija s cijanobakterijama uzetog u c) pri relativnoj centrifugalnoj sili u rasponu od 5,000 do 15,000 x g u vremenu od 5 do 30 minuta i dobivanje tih cijanobakterija iz granula koje nastanu centrifugiranjem.
2. Postupak prema zahtjevu 1, gdje je temperatura kulture u rasponu od 15 do 25°C.
3. Postupak prema zahtjevu 2, gdje je ta temperatura 22±2°C.
4. Postupak prema zahtjevu 1, gdje medij kulture ima konačnu koncentraciju arginina 2.8 mM.
5. Postupak prema zahtjevu 1, gdje medij kulture ima konačnu koncentraciju metionina 1.7 mM.
6. Postupak prema zahtjevu 1, gdje medij kulture ima konačnu koncentraciju alantoinske kiseline 1.0 mM.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CL2009000722A CL2009000722A1 (es) | 2009-03-24 | 2009-03-24 | Metodo de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes, utilizando un medio de cultivo mla suplementado con arginina, metionina y ácido alantoico. |
| PCT/IB2010/051188 WO2010109387A1 (es) | 2009-03-24 | 2010-03-18 | Método de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes |
| EP10755519.5A EP2412796B1 (en) | 2009-03-24 | 2010-03-18 | Method for the obtention and mass production of an isolated cyanobacteria species producing paralysing phycotoxins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20141069T1 true HRP20141069T1 (hr) | 2015-01-30 |
Family
ID=42780211
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20141069AT HRP20141069T1 (hr) | 2009-03-24 | 2014-11-03 | Postupak dobivanja i proizvodnje na veliko izoliranih vrsta cijanobakterija koje proizvode paralizirajuä†e fikotoksine |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP2412796B1 (hr) |
| JP (1) | JP5757044B2 (hr) |
| KR (1) | KR101296533B1 (hr) |
| AR (1) | AR076144A1 (hr) |
| BR (1) | BRPI1013549B1 (hr) |
| CA (1) | CA2754158C (hr) |
| CL (1) | CL2009000722A1 (hr) |
| DK (1) | DK2412796T3 (hr) |
| ES (1) | ES2523373T3 (hr) |
| HR (1) | HRP20141069T1 (hr) |
| MX (1) | MX2011010019A (hr) |
| PE (1) | PE20110908A1 (hr) |
| PT (1) | PT2412796E (hr) |
| WO (1) | WO2010109387A1 (hr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8957207B2 (en) | 2009-03-24 | 2015-02-17 | Proteus S.A. | Methods for producing phycotoxins |
| PE20160011A1 (es) | 2013-03-15 | 2016-02-10 | Childrens Medical Center | Formulaciones combinadas de neosaxitoxina para una anestesia local prolongada |
| CN108949847B (zh) * | 2018-08-01 | 2021-06-18 | 浙江海洋大学 | 一种利用海洋细菌发酵制备膝沟藻毒素的方法 |
| CN112110930B (zh) * | 2020-09-16 | 2022-03-29 | 中国水产科学研究院东海水产研究所 | 一种从有毒贝壳中提取新石房蛤毒素的方法 |
-
2009
- 2009-03-24 CL CL2009000722A patent/CL2009000722A1/es unknown
-
2010
- 2010-03-18 ES ES10755519.5T patent/ES2523373T3/es active Active
- 2010-03-18 BR BRPI1013549A patent/BRPI1013549B1/pt active IP Right Grant
- 2010-03-18 WO PCT/IB2010/051188 patent/WO2010109387A1/es active Application Filing
- 2010-03-18 DK DK10755519.5T patent/DK2412796T3/en active
- 2010-03-18 MX MX2011010019A patent/MX2011010019A/es active IP Right Grant
- 2010-03-18 EP EP10755519.5A patent/EP2412796B1/en active Active
- 2010-03-18 KR KR1020117021026A patent/KR101296533B1/ko active IP Right Grant
- 2010-03-18 JP JP2012501445A patent/JP5757044B2/ja active Active
- 2010-03-18 CA CA2754158A patent/CA2754158C/en active Active
- 2010-03-18 PT PT107555195T patent/PT2412796E/pt unknown
- 2010-03-18 PE PE2011001286A patent/PE20110908A1/es active IP Right Grant
- 2010-03-23 AR ARP100100935A patent/AR076144A1/es active IP Right Grant
-
2014
- 2014-11-03 HR HRP20141069AT patent/HRP20141069T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010109387A1 (es) | 2010-09-30 |
| ES2523373T3 (es) | 2014-11-25 |
| JP5757044B2 (ja) | 2015-07-29 |
| KR101296533B1 (ko) | 2013-08-13 |
| EP2412796A1 (en) | 2012-02-01 |
| PT2412796E (pt) | 2014-11-12 |
| BRPI1013549B1 (pt) | 2018-09-25 |
| EP2412796B1 (en) | 2014-08-06 |
| MX2011010019A (es) | 2011-10-11 |
| AR076144A1 (es) | 2011-05-18 |
| EP2412796A4 (en) | 2013-02-27 |
| CA2754158A1 (en) | 2010-09-30 |
| KR20110132368A (ko) | 2011-12-07 |
| PE20110908A1 (es) | 2012-01-19 |
| DK2412796T3 (en) | 2014-11-17 |
| BRPI1013549A2 (pt) | 2015-08-25 |
| CA2754158C (en) | 2015-07-14 |
| JP2012521212A (ja) | 2012-09-13 |
| CL2009000722A1 (es) | 2009-06-19 |
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