CL2009000722A1 - Metodo de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes, utilizando un medio de cultivo mla suplementado con arginina, metionina y ácido alantoico. - Google Patents

Metodo de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes, utilizando un medio de cultivo mla suplementado con arginina, metionina y ácido alantoico.

Info

Publication number
CL2009000722A1
CL2009000722A1 CL2009000722A CL2009000722A CL2009000722A1 CL 2009000722 A1 CL2009000722 A1 CL 2009000722A1 CL 2009000722 A CL2009000722 A CL 2009000722A CL 2009000722 A CL2009000722 A CL 2009000722A CL 2009000722 A1 CL2009000722 A1 CL 2009000722A1
Authority
CL
Chile
Prior art keywords
species
obtaining
mass production
ficotoxins
methionine
Prior art date
Application number
CL2009000722A
Other languages
English (en)
Inventor
Gonzalez Marcelo Santiago Lagos
Original Assignee
Proteus Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteus Sa filed Critical Proteus Sa
Priority to CL2009000722A priority Critical patent/CL2009000722A1/es
Publication of CL2009000722A1 publication Critical patent/CL2009000722A1/es
Priority to EP10755519.5A priority patent/EP2412796B1/en
Priority to PCT/IB2010/051188 priority patent/WO2010109387A1/es
Priority to KR1020117021026A priority patent/KR101296533B1/ko
Priority to PE2011001286A priority patent/PE20110908A1/es
Priority to MX2011010019A priority patent/MX2011010019A/es
Priority to JP2012501445A priority patent/JP5757044B2/ja
Priority to DK10755519.5T priority patent/DK2412796T3/en
Priority to PT107555195T priority patent/PT2412796E/pt
Priority to BRPI1013549A priority patent/BRPI1013549B1/pt
Priority to ES10755519.5T priority patent/ES2523373T3/es
Priority to CA2754158A priority patent/CA2754158C/en
Priority to ARP100100935A priority patent/AR076144A1/es
Priority to US13/243,119 priority patent/US8957207B2/en
Priority to US13/243,153 priority patent/US8952152B2/en
Priority to HRP20141069AT priority patent/HRP20141069T1/hr
Priority to US14/570,360 priority patent/US9249150B2/en
Priority to US14/570,373 priority patent/US20150099879A1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12Unicellular algae; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

LA PRESENTE INVENCIÓN SE RELACIONA CON UN MÉTODO DE OBTENCIÓN Y PRODUCCIÓN MASIVA DE UNA ESPECIE DE<br /> CIANOBACTERIA AISLADA PRODUCTORA DE FICOTOXINAS PARALIZANTES, QUE COMPRENDE LOS PASOS DE INOCULAR FILAMENTOS<br /> DE CIANOBACTERIA EN MEDIO DE CULTIVO MLA COMPLEMENTADO CON ARGININA, METIONINA Y ÁCIDO ALANTOICO; CULTIVAR<br /> LA CIANOBACTERIA; RECOLECTAR UN VOLUMEN DE MEDIO DE CULTIVO Y REPONER EL VOLUMEN RETIRADO CON MEDIO DE CULTIVO FRESCO; MANTENER EL CULTIVO EN MODO CONTINUO; CENTRIFUGAR EL MEDIO CON LAS CIANOBACTERIAS.<br />  
CL2009000722A 2009-03-24 2009-03-24 Metodo de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes, utilizando un medio de cultivo mla suplementado con arginina, metionina y ácido alantoico. CL2009000722A1 (es)

Priority Applications (18)

Application Number Priority Date Filing Date Title
CL2009000722A CL2009000722A1 (es) 2009-03-24 2009-03-24 Metodo de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes, utilizando un medio de cultivo mla suplementado con arginina, metionina y ácido alantoico.
CA2754158A CA2754158C (en) 2009-03-24 2010-03-18 Method to obtain and to produce massively an isolated species of cyanobacteria that produces paralyzing phycotoxins
PT107555195T PT2412796E (pt) 2009-03-24 2010-03-18 Método para a obtenção e produção em massa de uma espécie isolada de cianobactérias produtora de ficotoxinas paralisantes
ES10755519.5T ES2523373T3 (es) 2009-03-24 2010-03-18 Método de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes
KR1020117021026A KR101296533B1 (ko) 2009-03-24 2010-03-18 마비성 조류독소를 생산하는 단리된 시아노박테리아 종의 대량 취득 및 생산 방법
PE2011001286A PE20110908A1 (es) 2009-03-24 2010-03-18 Metodo de obtencion y produccion masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes
MX2011010019A MX2011010019A (es) 2009-03-24 2010-03-18 Metodo de obtencion y produccion masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes.
JP2012501445A JP5757044B2 (ja) 2009-03-24 2010-03-18 麻痺性藻類毒素を産生するシアノバクテリア分離種の大量取得生産方法
DK10755519.5T DK2412796T3 (en) 2009-03-24 2010-03-18 A method for obtaining and mass production of an isolated cyano-bacterial species which produce paralyzing phycotoksiner
EP10755519.5A EP2412796B1 (en) 2009-03-24 2010-03-18 Method for the obtention and mass production of an isolated cyanobacteria species producing paralysing phycotoxins
BRPI1013549A BRPI1013549B1 (pt) 2009-03-24 2010-03-18 método de obtenção e produção maciça de uma espécie de cianobactéria isolada produtora de ficotoxinas paralisantes
PCT/IB2010/051188 WO2010109387A1 (es) 2009-03-24 2010-03-18 Método de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes
ARP100100935A AR076144A1 (es) 2009-03-24 2010-03-23 Metodo de obtencion y produccion masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes
US13/243,119 US8957207B2 (en) 2009-03-24 2011-09-23 Methods for producing phycotoxins
US13/243,153 US8952152B2 (en) 2009-03-24 2011-09-23 Methods for purifying phycotoxins, pharmaceutical compositions containing purified phycotoxins, and methods of use thereof
HRP20141069AT HRP20141069T1 (hr) 2009-03-24 2014-11-03 Postupak dobivanja i proizvodnje na veliko izoliranih vrsta cijanobakterija koje proizvode paralizirajuä†e fikotoksine
US14/570,360 US9249150B2 (en) 2009-03-24 2014-12-15 Methods for purifying phycotoxins, pharmaceutical compositions containing purified phycotoxins, and methods of use thereof
US14/570,373 US20150099879A1 (en) 2009-03-24 2014-12-15 Methods for producing phycotoxins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2009000722A CL2009000722A1 (es) 2009-03-24 2009-03-24 Metodo de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes, utilizando un medio de cultivo mla suplementado con arginina, metionina y ácido alantoico.

Publications (1)

Publication Number Publication Date
CL2009000722A1 true CL2009000722A1 (es) 2009-06-19

Family

ID=42780211

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2009000722A CL2009000722A1 (es) 2009-03-24 2009-03-24 Metodo de obtención y producción masiva de una especie de cianobacteria aislada productora de ficotoxinas paralizantes, utilizando un medio de cultivo mla suplementado con arginina, metionina y ácido alantoico.

Country Status (14)

Country Link
EP (1) EP2412796B1 (es)
JP (1) JP5757044B2 (es)
KR (1) KR101296533B1 (es)
AR (1) AR076144A1 (es)
BR (1) BRPI1013549B1 (es)
CA (1) CA2754158C (es)
CL (1) CL2009000722A1 (es)
DK (1) DK2412796T3 (es)
ES (1) ES2523373T3 (es)
HR (1) HRP20141069T1 (es)
MX (1) MX2011010019A (es)
PE (1) PE20110908A1 (es)
PT (1) PT2412796E (es)
WO (1) WO2010109387A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108949847A (zh) * 2018-08-01 2018-12-07 浙江海洋大学 一种利用海洋细菌发酵制备膝沟藻毒素的方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8957207B2 (en) 2009-03-24 2015-02-17 Proteus S.A. Methods for producing phycotoxins
PE20160011A1 (es) 2013-03-15 2016-02-10 Childrens Medical Center Formulaciones combinadas de neosaxitoxina para una anestesia local prolongada
CN112110930B (zh) * 2020-09-16 2022-03-29 中国水产科学研究院东海水产研究所 一种从有毒贝壳中提取新石房蛤毒素的方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108949847A (zh) * 2018-08-01 2018-12-07 浙江海洋大学 一种利用海洋细菌发酵制备膝沟藻毒素的方法
CN108949847B (zh) * 2018-08-01 2021-06-18 浙江海洋大学 一种利用海洋细菌发酵制备膝沟藻毒素的方法

Also Published As

Publication number Publication date
WO2010109387A1 (es) 2010-09-30
ES2523373T3 (es) 2014-11-25
JP5757044B2 (ja) 2015-07-29
KR101296533B1 (ko) 2013-08-13
EP2412796A1 (en) 2012-02-01
PT2412796E (pt) 2014-11-12
BRPI1013549B1 (pt) 2018-09-25
HRP20141069T1 (hr) 2015-01-30
EP2412796B1 (en) 2014-08-06
MX2011010019A (es) 2011-10-11
AR076144A1 (es) 2011-05-18
EP2412796A4 (en) 2013-02-27
CA2754158A1 (en) 2010-09-30
KR20110132368A (ko) 2011-12-07
PE20110908A1 (es) 2012-01-19
DK2412796T3 (en) 2014-11-17
BRPI1013549A2 (pt) 2015-08-25
CA2754158C (en) 2015-07-14
JP2012521212A (ja) 2012-09-13

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