CL2019003459A1 - Métodos de cultivo celular. - Google Patents

Métodos de cultivo celular.

Info

Publication number
CL2019003459A1
CL2019003459A1 CL2019003459A CL2019003459A CL2019003459A1 CL 2019003459 A1 CL2019003459 A1 CL 2019003459A1 CL 2019003459 A CL2019003459 A CL 2019003459A CL 2019003459 A CL2019003459 A CL 2019003459A CL 2019003459 A1 CL2019003459 A1 CL 2019003459A1
Authority
CL
Chile
Prior art keywords
recombinant protein
weight
cell culture
total
produced
Prior art date
Application number
CL2019003459A
Other languages
English (en)
Inventor
Andrew Jeffrey Yates
Yahia Bassem Ben
Laetitia Malphettes
Nadine Kochanowski
Gill Renner
Sandrine Durran
Original Assignee
Ucb Biopharma Sprl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb Biopharma Sprl filed Critical Ucb Biopharma Sprl
Publication of CL2019003459A1 publication Critical patent/CL2019003459A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Abstract

UN PROCESO PARA PRODUCIR UNA PROTEÍNA RECOMBINANTE, QUE COMPRENDE: A. CULTIVAR CÉLULAS HOSPEDERAS CAPACES DE PRODUCIR UNA PROTEÍNA RECOMBINANTE EN UN MEDIO; B. HACER PROGRESAR EL CULTIVO A TRAVÉS DE UNA FASE DE PRODUCCIÓN EN DONDE LA PROTEÍNA RECOMBINANTE ES PRODUCIDA POR LAS CÉLULAS, EN DONDE, DURANTE LA FASE DE PRODUCCIÓN, EL CULTIVO SE COMPLEMENTA CON • CISTEÍNA O CISTINA HASTA UNA CANTIDAD TOTAL DE 10% EN PESO A 30% EN PESO DE LA CANTIDAD TOTAL ESPERADA DE LA PROTEÍNA RECOMBINANTE PRODUCIDA; Y/O • TRIPTÓFANO HASTA UNA CANTIDAD TOTAL DE 8% EN PESO A 35% EN PESO DE LA CANTIDAD TOTAL ESPERADA DE LA PROTEÍNA RECOMBINANTE PRODUCIDA, C. Y, OPCIONALMENTE, RECUPERAR LA PROTEÍNA RECOMBINANTE DEL MEDIO DE CULTIVO CELULAR.
CL2019003459A 2017-05-31 2019-11-26 Métodos de cultivo celular. CL2019003459A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB1708655.4A GB201708655D0 (en) 2017-05-31 2017-05-31 Cell culture methods

Publications (1)

Publication Number Publication Date
CL2019003459A1 true CL2019003459A1 (es) 2020-04-24

Family

ID=59270991

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2019003459A CL2019003459A1 (es) 2017-05-31 2019-11-26 Métodos de cultivo celular.

Country Status (17)

Country Link
US (1) US20200239923A1 (es)
EP (1) EP3630946B1 (es)
JP (3) JP7460370B2 (es)
KR (1) KR20200012972A (es)
CN (1) CN110741077A (es)
AU (1) AU2018276376A1 (es)
BR (1) BR112019024390A2 (es)
CA (1) CA3065661A1 (es)
CL (1) CL2019003459A1 (es)
CO (1) CO2019012871A2 (es)
EA (1) EA201992828A1 (es)
GB (1) GB201708655D0 (es)
IL (1) IL270880A (es)
MX (1) MX2019014140A (es)
MY (1) MY193535A (es)
RU (2) RU2758674C2 (es)
WO (1) WO2018219968A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201708655D0 (en) 2017-05-31 2017-07-12 Ucb Biopharma Sprl Cell culture methods
IL303951A (en) * 2020-12-22 2023-08-01 Novartis Ag Methods for reducing the oxidation level of cysteine residues in recombinantly secreted protein during cell culture
GB202105424D0 (en) 2021-04-16 2021-06-02 UCB Biopharma SRL Cell culture processes

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101523782B1 (ko) 2006-11-08 2015-05-28 와이어쓰 엘엘씨 세포 배양을 위한 합리적으로 설계된 배지
US8030027B2 (en) * 2008-04-29 2011-10-04 St. Jude Children's Research Hospital Method for enhancing recombinant antibody production
AU2011246504B2 (en) * 2010-04-26 2013-09-26 Novartis Ag Improved cell culture medium
WO2012145682A1 (en) * 2011-04-21 2012-10-26 Amgen Inc. A method for culturing mammalian cells to improve recombinant protein production
JP6265970B2 (ja) 2012-03-26 2018-01-24 サノフイ 安定なＩｇＧ４に基づく結合剤の製剤
WO2013158275A1 (en) * 2012-04-20 2013-10-24 Abbvie Inc. Cell culture methods to reduce acidic species
GB201208370D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Antibodies
GB201208367D0 (en) * 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
RU2015144020A (ru) * 2013-03-15 2017-04-21 Дженентек, Инк. Среды для культивирования клеток и способы получения антител
GB201508180D0 (en) * 2015-05-13 2015-06-24 Ucb Biopharma Sprl Antibodies
US11220668B2 (en) * 2016-04-28 2022-01-11 Merck Patent Gmbh Method for reducing the trisulfide level in proteins
GB201608323D0 (en) * 2016-05-12 2016-06-29 Ucb Biopharma Sprl Pharmaceutical compositions
WO2018018613A1 (zh) * 2016-07-29 2018-02-01 广东东阳光药业有限公司 一种提高抗体纯度的细胞培养基和培养方法
GB201708655D0 (en) 2017-05-31 2017-07-12 Ucb Biopharma Sprl Cell culture methods

Also Published As

Publication number Publication date
MX2019014140A (es) 2020-02-07
RU2021128024A (ru) 2021-12-08
RU2758674C2 (ru) 2021-11-01
RU2019143742A3 (es) 2021-06-30
JP7460370B2 (ja) 2024-04-02
JP2021185185A (ja) 2021-12-09
IL270880A (en) 2020-01-30
CA3065661A1 (en) 2018-12-06
JP2020521490A (ja) 2020-07-27
EA201992828A1 (ru) 2020-04-21
KR20200012972A (ko) 2020-02-05
JP2022153421A (ja) 2022-10-12
MY193535A (en) 2022-10-18
GB201708655D0 (en) 2017-07-12
WO2018219968A1 (en) 2018-12-06
BR112019024390A2 (pt) 2020-07-14
CN110741077A (zh) 2020-01-31
EP3630946B1 (en) 2024-03-27
RU2019143742A (ru) 2021-06-30
AU2018276376A1 (en) 2019-12-12
CO2019012871A2 (es) 2020-01-17
US20200239923A1 (en) 2020-07-30
JP7406593B2 (ja) 2023-12-27
EP3630946A1 (en) 2020-04-08

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