NZ538022A - A method of culturing pearls stimulated by nacre-originating proteins - Google Patents
A method of culturing pearls stimulated by nacre-originating proteinsInfo
- Publication number
- NZ538022A NZ538022A NZ538022A NZ53802203A NZ538022A NZ 538022 A NZ538022 A NZ 538022A NZ 538022 A NZ538022 A NZ 538022A NZ 53802203 A NZ53802203 A NZ 53802203A NZ 538022 A NZ538022 A NZ 538022A
- Authority
- NZ
- New Zealand
- Prior art keywords
- nacre
- oyster
- species
- genus
- gonad
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 32
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 11
- 239000011049 pearl Substances 0.000 title claims description 21
- 238000012258 culturing Methods 0.000 title claims description 10
- 210000002149 gonad Anatomy 0.000 claims abstract description 24
- 241000237852 Mollusca Species 0.000 claims abstract description 17
- 230000004936 stimulating effect Effects 0.000 claims description 36
- 241000237502 Ostreidae Species 0.000 claims description 34
- 235000020636 oyster Nutrition 0.000 claims description 34
- 241000490567 Pinctada Species 0.000 claims description 17
- 241000894007 species Species 0.000 claims description 12
- 241000217407 Margaritifera Species 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000003086 colorant Substances 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 2
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims 23
- 239000008346 aqueous phase Substances 0.000 claims 2
- 238000002156 mixing Methods 0.000 claims 2
- 239000008223 sterile water Substances 0.000 claims 2
- 241000237891 Haliotidae Species 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 238000012136 culture method Methods 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a pearl culture method employing a recipient pearl-producing mollusc with a gonad. The inventive method consists in introducing a nucleus and a stimulant solution containing proteins and associated molecules of mother-of-pearl origin into the gonad of the pearl-producing mollusc.
Description
New Zealand Paient Spedficaiion for Paient Number 538022
538022
A METHOD OF CULTURING PEARLS STIMULATED BY NACRE-ORIGINATING PROTEINS
The present invention relates to a method of culturing pearls.
It is known that a nacre-producing receiver mollusk is used to culture pearls, said mollusk generally being a receiver oyster having a gonad pouch into which a nucleus is introduced. During initial grafting, i.e. when the receiver oyster receives a nucleus for the first time, a 10 graft is also added in the form of a small piece of mantle from a donor oyster. During subsequent grafting, i.e. when the receiver oyster has already produced a first pearl, a second nucleus is introduced into the gonad pouch of the receiver oyster, and generally it is 15 not necessary to insert another graft.
Whether during initial grafting or subsequent grafting, in general no significant deposit of nacre is obtained until a little more than one year has elapsed since the nucleus was introduced, and in order to obtain 20 a quality pearl it is generally necessary to wait at least two years.
An object of the invention is to provide a method making it possible to accelerate the formation of the deposit of nacre on the nucleus and to improve its 25 quality, or to at least provide the public with a useful alternative.
The invention provides a method of culturing pearls by using a nacre-producing receiver mollusk having a gonad pouch, the method comprising a step of introducing 30 a nucleus into the gonad pouch of the nacre-producing mollusk, said method being wherein it further comprises the step of introducing a stimulating solution of associated nacre-originating proteins and molecules into the gonad pouch.
By adding this step to the traditional grafting method, whether during initial grafting or subsequent grafting, it is found that formation of the nacre deposit
Intellectual Property Office of N.2.
H DEC 2006
(Followed by page la)
la accelerates substantially in so far as a significant deposit of nacre is obtained only a few months after the nucleus is introduced into the gonad pouch of the receiver oyster.
In order to implement the method of the invention, it is appropriate to begin by preparing the stimulating
2
solution containing the associated nacre-originating proteins and molecules. When culturing pearls using an oyster, firstly a plate of nacre is obtained by sanding off the outer layers of an oyster shell until the 5 iridescent color of the nacre appears. Then, plates of descaled nacre are reduced to powder, for example, by sawing and grinding said plates of nacre, and it is preferable to select the powder fraction that has a grain size of less than 50 Jim. The resulting nacre powder is 10 put into contact with ultra-pure or pyrogen-free water by soaking the powder in ultra-pure water, at a concentration in the range of 25 grams (g) to 100 g of powder per 100 milliliters (mL) of water, depending on the grain size of the powder, the mixture being stirred 15 continuously for about twenty hours. The resulting mixture is centrifuged and then filtered in order to separate the solid portion from the liquid portion and retain only the liquid portion which makes up the stimulating solution containing the associated proteins 20 and molecules.
The method of the invention can then be implemented in different ways. In a preferred first implementation, the nucleus is soaked in the stimulating solution prior to being introduced into the gonad pouch of the pearl-25 producing oyster.
In a second implementation that can be performed during initial grafting, the graft is soaked in the stimulating solution prior to being introduced into the gonad pouch. In this case, it is observed that it is 30 also possible to soak both the nucleus and the graft in the stimulating solution before they are introduced into the gonad pouch.
In a third implementation, the stimulating solution is injected into the gonad pouch before or after the 35 nucleus is introduced. As in the second implementation, the stimulating solution can be injected while
3
simultaneously coating the nucleus and/or the graft with the stimulating solution.
In another aspect of the invention, the stimulating solution is also used in order to vary the color of the 5 pearl obtained. It can be seen that in the implementation of the method described above for obtaining the stimulating solution the entire plate of nacre of an oyster is used to produce the nacre powder from which the associated proteins and molecules are 10 extracted. This means that proteins are extracted not only from the white portion of the nacre but also from the colored portion, which is representative of the genus and of the species of the oyster from which the nacre is extracted.
In order to obtain pearls of different colors from a single donor oyster, or, more generally, from a single nacre-producing mollusk, it is possible to prepare the stimulating solution from nacre of a pre-selected color, either by using a particular zone of the shell, or by 20 using nacre coming from an oyster that is a different color from that of the receiving oyster.
By way of example, a stimulating solution prepared from an oyster of the Pinctada genus, Margaritifera species albinos variant, used with a receiver oyster of 25 the Pinctada genus, normal Margaritifera species serves to obtain a pearl presenting a very soft silver grey orient with pastel-colored reflections of dapple grey type.
Likewise, with a stimulating solution prepared from 3 0 the nacre of an oyster of the Pinctada genus Maculata species and used in conjunction with a receiver oyster of the Pinctada genus, normal Margaritifera species, the pearl obtained is a golden pearl with dark reflections having a very iridescent orient.
Of course, the invention is not limited to the implementations described and it is possible to vary
Claims (14)
1. A method of culturing pearls by using a nacre-producing receiver mollusk having a gonad pouch, the method comprising a step of introducing a nucleus into 5 the gonad pouch of the nacre-producing mollusk, said method being wherein it further comprises the step of introducing a stimulating solution of associated nacre-originating proteins and molecules into the gonad pouch. 10
2. A method according to claim 1, wherein the stimulating solution is introduced into the gonad pouch by the nucleus being soaked in said stimulating solution prior to being introduced into the gonad pouch. 15
3. A method according to claim 1 or claim 2, wherein it further comprises the step of introducing a graft into the gonad pouch, and in that the stimulating solution is introduced into the gonad pouch by the graft being soaked in said stimulating solution prior to being introduced 20 into the gonad pouch.
4. A method according to claim 1, wherein the stimulating solution is injected into the gonad pouch. 25
5. A method according to any one of claims 1 to 4, wherein the stimulating solution is obtained by reducing the nacre to powder on the basis of a grain size of less than 50 pm, and by putting the resulting powder into contact with ultra-pure water at a concentration in the 30 range of 25 g to 100 g of powder per 100 mL of water, followed by extraction of the aqueous phase.
6. A method according to claim 5, wherein the aqueous phase is lyophilized, and wherein a stimulating solution 35 is reconstituted by mixing 50 mg to 150 mg in 20 mL to 50 mL of sterile water, depending on the method used to implement the stimulating solution. Intellectual Property Office of N.Z. ' 1 DEC 2006 6
7. A method according to claim 6, wherein the stimulating solution is reconstituted by mixing 100 mg in 20 mL to 50 mL of sterile water. 5
8. A method according to any one of claims 1 to 7, wherein the stimulating solution is prepared from nacre having a pre-selected color. 10
9. A method according to claim 8, wherein the stimulating solution is extracted from the nacre of an oyster of the Pinctada genus, Margaritifera albinos species, and the receiver nacre-producing mollusk is an oyster of the Pinctada genus, normal Margaritifera species. 15
10. A method according to claim 8, wherein the stimulating solution is extracted from the nacre of an oyster of the Pinctada genus, normal Maculata species, and the receiver mollusk is an oyster of the Pinctada 20 genus, normal Margaritifera species.
11. A method according to claim 8, wherein the stimulating solution is extracted from the nacre of an oyster of the Pinctada genus, normal Margaritifera 25 species, and the receiver nacre-producing mollusk is an oyster of the Pinctada genus, normal Fucata species.
12. A method according to claim 8, wherein the stimulating solution is extracted from the nacre of an 30 oyster of the Pinctada genus, Maculata species or Margaritifera species and the nacre-producing receiver mollusk is an oyster of the Pinctada genus, Maxima species. 35
13. A method according to claim 8, wherein the stimulating solution is extracted from the nacre of an oyster of the Pinctada genus, Maxima species, and the Intellectual Property Office of N.2. 11 DEC 2006 7 nacre-producing receiver mollusk is an oyster of the Pinctada genus, Margaritifera species.
14. A pearl whenever produced by the method according to 5 any one of claims 1 to 13. ROBERT WAN HOLDING 53 8 0 ^ \ 8 \ ABSTRACT A METHOD OF CULTURING PEARLS STIMULATED BY NACRE-ORIGINATING PROTEINS 5 \ A method of culturing pearls is implemented by using a nacre-producing receiver mollusk having a gonad pouch, the method comprising Yhe steps of introducing a nucleus and a stimulating solution of associated nacre-10 originating proteins and Volecules into the gonad pouch of the nacre-producing mollusk. 15 20 25 30 Translation of the title and the abstract as they were when originally filed by the 35 Applicant. No account has been taken of any changes that may have been made subsequently by the PCT Authorities acting ex officio, e.g. under PCT Rules 37.2, 33.2, and/or 48.3.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0210213A FR2843277B1 (en) | 2002-08-12 | 2002-08-12 | PROCESS FOR THE CULTURE OF PEARLS STIMULATED BY PROTEINS OF NACRIOR ORIGIN |
PCT/FR2003/002172 WO2004016078A1 (en) | 2002-08-12 | 2003-07-10 | Method for pearl culture stimulated by proteins of mother-of-pearl origin |
Publications (1)
Publication Number | Publication Date |
---|---|
NZ538022A true NZ538022A (en) | 2007-02-23 |
Family
ID=30471073
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NZ538022A NZ538022A (en) | 2002-08-12 | 2003-07-10 | A method of culturing pearls stimulated by nacre-originating proteins |
Country Status (4)
Country | Link |
---|---|
AU (1) | AU2003284974B2 (en) |
FR (1) | FR2843277B1 (en) |
NZ (1) | NZ538022A (en) |
WO (1) | WO2004016078A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005063005A1 (en) * | 2003-11-28 | 2005-07-14 | Robert Wan Holding | Pearl culture method stimulated by proteins of mother-of-pearl origin |
FR2866201B1 (en) * | 2004-02-13 | 2006-12-08 | Robert Wan Holding | PROCESS FOR CULTIVATION OF PEARLS STIMULATED BY EXTRAPALLEAL LIQUID |
FR2889793B1 (en) * | 2005-08-17 | 2007-10-05 | Robert Wan Holding | METHOD OF IMPROVING THE SURVIVAL OF OYSTER LARVAES WHEN FIXED, AND NOURISHING SOLUTION FOR CARRYING OUT SAID METHOD. |
CN103975883B (en) * | 2014-05-23 | 2016-01-20 | 广东海洋大学 | The selection of the black shell strain of a kind of pteria martensii for cultivating sea water pearls |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0556729A (en) * | 1991-08-31 | 1993-03-09 | Taiyo Fishery Co Ltd | Method for promoting growth of pearl |
JPH10108582A (en) * | 1996-10-08 | 1998-04-28 | Tazaki Shinjiyu Kk | Nucleus insertion method for cultured pearl |
-
2002
- 2002-08-12 FR FR0210213A patent/FR2843277B1/en not_active Expired - Fee Related
-
2003
- 2003-07-10 AU AU2003284974A patent/AU2003284974B2/en not_active Ceased
- 2003-07-10 WO PCT/FR2003/002172 patent/WO2004016078A1/en active IP Right Grant
- 2003-07-10 NZ NZ538022A patent/NZ538022A/en unknown
Also Published As
Publication number | Publication date |
---|---|
AU2003284974B2 (en) | 2007-06-14 |
WO2004016078A1 (en) | 2004-02-26 |
FR2843277B1 (en) | 2004-10-22 |
FR2843277A1 (en) | 2004-02-13 |
AU2003284974A1 (en) | 2004-03-03 |
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Legal Events
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PSEA | Patent sealed | ||
RENW | Renewal (renewal fees accepted) |