WO2005063005A1 - Pearl culture method stimulated by proteins of mother-of-pearl origin - Google Patents
Pearl culture method stimulated by proteins of mother-of-pearl origin Download PDFInfo
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- WO2005063005A1 WO2005063005A1 PCT/FR2003/003520 FR0303520W WO2005063005A1 WO 2005063005 A1 WO2005063005 A1 WO 2005063005A1 FR 0303520 W FR0303520 W FR 0303520W WO 2005063005 A1 WO2005063005 A1 WO 2005063005A1
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- WIPO (PCT)
- Prior art keywords
- pearl
- oyster
- stimulating solution
- gonadal
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 9
- 238000012136 culture method Methods 0.000 title abstract 2
- 241000237852 Mollusca Species 0.000 claims abstract description 11
- 239000011049 pearl Substances 0.000 claims description 40
- 230000004936 stimulating effect Effects 0.000 claims description 33
- 241000237502 Ostreidae Species 0.000 claims description 32
- 235000020636 oyster Nutrition 0.000 claims description 32
- 238000000034 method Methods 0.000 claims description 19
- 230000002710 gonadal effect Effects 0.000 claims description 18
- 241000490567 Pinctada Species 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 10
- 241000217407 Margaritifera Species 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 239000008346 aqueous phase Substances 0.000 claims 2
- 241001490476 Pinctada maxima Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 235000015170 shellfish Nutrition 0.000 claims 1
- 239000008223 sterile water Substances 0.000 claims 1
- 210000002149 gonad Anatomy 0.000 abstract 2
- 230000000638 stimulation Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 20
- 239000003086 colorant Substances 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Definitions
- Pearl cultivation process stimulated with proteins of pearl origin.
- the present invention relates to a pearl cultivation method. It is known that to carry out the cultivation of pearls a receiving pearl mollusc is used, generally a recipient oyster, having a gonadal pocket into which a nucleus is introduced.
- a graft is also added in the form of a small piece of mantle from a donor oyster.
- a second nucleus is introduced into the gonadal pocket of the recipient oyster and it is generally not necessary to deliver a graft.
- An object of the invention is to propose a method making it possible to accelerate the formation and improve the quality of the deposition of nacre on the nucleus.
- a method is proposed comprising the step of introducing into the gonadal pocket a stimulating solution of proteins and associated molecules of pearly origin.
- a powder reduction is then carried out, for example by sawing off the mother-of-pearl plates and grinding, and the fraction of powder with a particle size less than 50 ⁇ m is preferably selected.
- the mother-of-pearl powder thus obtained is brought into contact with ultrapure or aspirogenic water by soaking the powder in ultrapure water, at a rate of 25 g to 100 g of powder per 100 ml of water depending on the particle size. powder, stirring the mixture continuously for about twenty hours.
- the mixture obtained is centrifuged then filtered to separate the solid part from the liquid part and keep only the liquid part which forms the stimulating solution containing the proteins and associated molecules.
- the method according to the invention can then be implemented in different ways.
- the nucleus is soaked in the stimulating solution before its introduction into the gonadal pocket of the pearl oyster.
- the graft is soaked in the stimulating solution before its introduction into the gonadal pocket. Note that in this case it is also possible to dip both the nucleus and the graft in the stimulating solution before their introduction into the gonadal pocket.
- an in- Stimulating solution is injected into the gonadal pocket before or after introduction of the nucleus.
- an injection of stimulating solution can be carried out in conjunction with covering the nucleus and / or the graft with the stimulating solution.
- the stimulating solution is also used to vary the color of the pearl obtained.
- the entire mother-of-pearl plate of an oyster is used to make the mother-of-pearl powder from which the proteins and associated molecules. This means that proteins will not only be extracted from the white part of the mother-of-pearl but also from the colored part which is representative of the genus and species of the oyster from which the mother-of-pearl is extracted.
- the stimulant solution can be produced from a mother-of-pearl of selected color, either by using a particular area of the shell, either by using the pro- nacre coming from a Gri 'be -having a color different from that oyster recipient.
- a stimulating solution produced from an oyster of the genus Pinctada, species Margaritifera variant albino, used with an oyster of the genus Pinctada species normal Margaritifera allows to obtain a pearl having a gray orient very soft silver with reflections of pastel colors like pigeon throat.
- the pearl obtained is a golden pearl with dark reflections having a very shimmering orient.
- the invention is not limited to the modes of implementation described and it is possible to make variant embodiments without departing from the scope of the invention as defined by the claims.
- mention has been made of direct use of the stimulating solution provision may be made to keep the stimulating solution in lyophilized form. In this case it is possible to act on the concentration of the stimulating solution during the rehydration of the lyophilisate.
- a solution obtained by diluting 50 mg to 150 mg, and preferably 100 mg, of lyophilisate in 50 ml of water is used while for an injection the same amounts of lyophilisate are diluted in 20 ml of water only.
- the invention has been described in relation to a recipient oyster of dark color and "stimulating solutions obtained from oysters of light color, provision may be made to invert these colors for example by using a stimulating solution extracted from an oyster of the genus Pinctada species Margaritifera introduced into the gonadal pocket of an oyster of the genus Pinctada species normal Fucata.
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a pearl culture method which is carried out by using a recipient pearl-producing mollusc having a gonad and consists in introducing, a nucleus and a stimulation solution containing protein and associated molecules of mother-of-pearl origin into the gonad of the pearl-producing mollusc.
Description
Procédé de culture de perles stimulée par des protéines d'origine nacrière. La présente invention concerne un procédé de culture de perles. On sait que pour réaliser la culture de perles on utilise un mollusque nacrier receveur, généralement une huître receveuse, ayant une poche gonadique dans laquelle est introduit un nucleus. Lors d'une greffe originale, c'est-à-dire lorsque l'huître receveuse reçoit un nucleus pour la première fois, on ajoute en outre un greffon sous forme d'un petit morceau de manteau d'une huître don- neuse. Lors d'une regreffe, c'est-à-dire lorsque l'huître receveuse a déjà produit une première perle, un second nucleus est introduit dans la poche gonadique de l'huître receveuse et il n'est généralement pas nécessaire de remettre un greffon. Qu'il s'agisse d'une greffe originale ou d'une regreffe, un dépôt significatif de nacre sur le nucleus n'est généralement obtenu qu'après un peu plus d'un an à partir de l'introduction du nucleus et pour obtenir une perle de qualité il est généralement nécessaire d'atten- dre au moins deux ans. Un but de l'invention est de proposer un procédé permettant d'accélérer la formation et améliorer la qualité du dépôt de nacre sur le nucleus. Selon l'invention, on propose un procédé compor- tant l'étape d'-introduire dans la poche gonadique une solution stimulante de protéines et de molécules associées d'origine nacrière. En ajoutant cette étape au procédé de greffe traditionnel, qu'il s'agisse d'une greffe originale ou d'une regreffe, on a constaté une accélération remarquable dans la formation du dépôt de nacre dans la mesure où un dépôt significatif de nacre a été obtenu quelques mois seule¬ ment après l'introduction du nucleus dans la poche gonadique de l'huître receveuse.
Pour mettre en œuvre le procédé selon l'invention, il convient tout d'abord de réaliser la solution stimulante contenant les protéines et les molécules associées d'origine nacrière. Dans le cas d'une cul- ture de perles en utilisant une huître, on réalise tout d'abord une plaque de nacre par ponçage des couches extérieures d'une coquille d'huître jusqu'à l'apparition de la couleur irisée de la nacre. On effectue ensuite une réduction en poudre, par exemple par sciage des plaques de nacre décroûtées et broyage, et on sélectionne de préférence la fraction de poudre de granulométrie inférieure à 50 μm. La poudre de nacre ainsi obtenue est mise en contact avec de l'eau ultrapure ou apirogène par trempage de la poudre dans l'eau ultrapure, à raison de 25 g à 100 g de poudre pour 100 ml d'eau en fonction de la granulométrie de la poudre, en agitant le mélange de façon continue pendant environ vingt heures. Le mélange obtenu est centrifugé puis filtré pour séparer la partie solide de la partie liquide et ne conserver que la partie liquide qui forme la solution stimulante contenant les protéines et les molécules associées. Le procédé selon l'invention peut alors être mis en œuvre de différentes façons. Selon un premier mode de mise en œuvre préféré, le nucleus est trempé dans la so- lution stimulante préalablement à son introduction dans la poche gonadique de l'huître perlière. Selon un second mode de mise en œuvre réalisable lors d'une greffe originale, le greffon est trempé dans la solution stimulante préalablement à son introduction dans la poche gonadique. On notera que dans ce cas il est également possible de tremper à la fois le nucleus et le greffon dans la solution stimulante avant leur introduction dans la poche gonadique. Selon un troisième mode de mise en œuvre, une in-
jection de solution stimulante est effectuée dans la poche gonadique avant ou après introduction du nucleus. Comme dans le cas précédent une injection de solution stimulante peut être réalisé conjointement à un recouvre- ment du nucleus et/ou du greffon avec la solution stimulante . Selon un autre aspect de l'invention, la solution stimulante est en outre utilisée pour faire varier la couleur de la perle obtenue. En effet, dans le mode de mise en œuvre décrit ci-dessus pour l'obtention de la solution stimulante, on remarquera que l'intégralité de la plaque de nacre d'une huître est utilisée pour réaliser la poudre de nacre dont sont extraites les protéines et les molécules associées. Ceci signifie que des protéines vont non seulement être extraites de la partie blanche de la nacre mais également de la partie colorée qui est représentative du genre et de l'espèce de l'huître dont la nacre est extraite. Pour obtenir des perles de différentes couleurs à partir d'une même huître donneuse, ou d'une façon plus générale à partir d'un même mollusque nacrier, on peut réaliser la solution stimulante à partir d'une nacre de couleur sélectionnée, soit en utilisant une zone particulière de la coquille, soit en utilisant de la nacre pro- venant d' une huî'tre -ayant une couleur différente de celle l'huître receveuse. A titre d'exemple, une solution stimulante réalisée à partir d'une huître du genre Pinctada, espèce Mar- garitifera variant albinos, utilisée avec une huître re- ceveuse du genre Pinctada espèce Margaritifera normale permet d'obtenir une perle présentant un orient gris argenté très doux avec des reflets de couleurs pastels type gorge de pigeon. De même, avec une solution stimulante réalisée à
partir de la nacre d'une huître du genre Pinctada espèce Maculata et utilisée en relation avec une huître receveuse du genre Pinctada espèce Margaritifera normale, la perle obtenue est une perle dorée à reflets sombres ayant un orient très chatoyant . Bien entendu, l'invention n'est pas limitée aux modes de mise en œuvre décrits et on peut y apporter des variantes de réalisation sans sortir du cadre de l'invention tel que défini par les revendications. En particulier, bien que dans les modes de mise en œuvre décrits il ait été fait mention d'une utilisation directe de la solution stimulante, on peut prévoir de conserver la solution stimulante sous forme lyophilisée. Dans ce cas il est possible d'agir sur la concentration de la solution stimulante lors de la réhydratation du lyophilisât. En particulier pour un trempage du nucleus ou du greffon, on utilise une solution obtenue en diluant 50 mg à 150 mg, et de préférence 100 mg, de lyophilisât dans 50 ml d'eau tandis que pour une injection les mêmes quantités de lyophilisât sont diluées dans 20 ml d'eau seulement. De même, en ce qui concerne la recherche de couleurs de perles variées, bien que l'invention ait été décrite en relation avec une huître receveuse de couleur sombre et des "solutions stimulantes obtenues à partir d'huîtres de couleur claire, on peut prévoir d'inverser ces couleurs par exemple en utilisant une solution stimulante extraite d'une huître du genre Pinctada espèce Margaritifera introduite dans la poche gonadique d'une huî- tre du genre Pinctada espèce Fucata normale. On peut également utiliser des huîtres d'un genre ou d'une espèce différente de ceux énoncés, par exemple une huître du genre Pinctada espèce Maxima. On obtient ainsi des perles claires pouvant présenter différentes nuances dorées ou
argentées . Bien que la description ait été faite plus particulièrement en relation avec la culture de perles dans les huîtres, on peut également envisager une culture de perles utilisant d'autres mollusques nacriers tels que des ormeaux.
Pearl cultivation process stimulated with proteins of pearl origin. The present invention relates to a pearl cultivation method. It is known that to carry out the cultivation of pearls a receiving pearl mollusc is used, generally a recipient oyster, having a gonadal pocket into which a nucleus is introduced. In an original transplant, that is to say when the recipient oyster receives a nucleus for the first time, a graft is also added in the form of a small piece of mantle from a donor oyster. During a transplant, that is to say when the recipient oyster has already produced a first pearl, a second nucleus is introduced into the gonadal pocket of the recipient oyster and it is generally not necessary to deliver a graft. Whether it is an original transplant or a transplant, a significant deposit of mother-of-pearl on the nucleus is generally obtained only after a little over a year from the introduction of the nucleus and for obtaining a quality pearl it is generally necessary to wait at least two years. An object of the invention is to propose a method making it possible to accelerate the formation and improve the quality of the deposition of nacre on the nucleus. According to the invention, a method is proposed comprising the step of introducing into the gonadal pocket a stimulating solution of proteins and associated molecules of pearly origin. By adding this step to the traditional grafting process, whether it is an original graft or a re-grafting, we noted a remarkable acceleration in the formation of the mother-of-pearl deposit insofar as a significant deposit of mother-of-pearl has obtained a few months only ¬ after the introduction of the nucleus in gonadal pocket oyster recipient. To implement the method according to the invention, it is first of all necessary to produce the stimulating solution containing the proteins and associated molecules of pearly origin. In the case of a pearl culture using an oyster, a mother-of-pearl plate is first produced by sanding the outer layers of an oyster shell until the iridescent color of the nacre. A powder reduction is then carried out, for example by sawing off the mother-of-pearl plates and grinding, and the fraction of powder with a particle size less than 50 μm is preferably selected. The mother-of-pearl powder thus obtained is brought into contact with ultrapure or aspirogenic water by soaking the powder in ultrapure water, at a rate of 25 g to 100 g of powder per 100 ml of water depending on the particle size. powder, stirring the mixture continuously for about twenty hours. The mixture obtained is centrifuged then filtered to separate the solid part from the liquid part and keep only the liquid part which forms the stimulating solution containing the proteins and associated molecules. The method according to the invention can then be implemented in different ways. According to a first preferred embodiment, the nucleus is soaked in the stimulating solution before its introduction into the gonadal pocket of the pearl oyster. According to a second mode of implementation achievable during an original graft, the graft is soaked in the stimulating solution before its introduction into the gonadal pocket. Note that in this case it is also possible to dip both the nucleus and the graft in the stimulating solution before their introduction into the gonadal pocket. According to a third mode of implementation, an in- Stimulating solution is injected into the gonadal pocket before or after introduction of the nucleus. As in the previous case, an injection of stimulating solution can be carried out in conjunction with covering the nucleus and / or the graft with the stimulating solution. According to another aspect of the invention, the stimulating solution is also used to vary the color of the pearl obtained. In fact, in the mode of implementation described above for obtaining the stimulating solution, it will be noted that the entire mother-of-pearl plate of an oyster is used to make the mother-of-pearl powder from which the proteins and associated molecules. This means that proteins will not only be extracted from the white part of the mother-of-pearl but also from the colored part which is representative of the genus and species of the oyster from which the mother-of-pearl is extracted. To obtain pearls of different colors from the same donor oyster, or more generally from the same mother-of-pearl mollusk, the stimulant solution can be produced from a mother-of-pearl of selected color, either by using a particular area of the shell, either by using the pro- nacre coming from a Gri 'be -having a color different from that oyster recipient. For example, a stimulating solution produced from an oyster of the genus Pinctada, species Margaritifera variant albino, used with an oyster of the genus Pinctada species normal Margaritifera allows to obtain a pearl having a gray orient very soft silver with reflections of pastel colors like pigeon throat. Similarly, with a stimulating solution made at from the nacre of an oyster of the genus Pinctada species Maculata and used in connection with a recipient oyster of the genus Pinctada species Margaritifera normal, the pearl obtained is a golden pearl with dark reflections having a very shimmering orient. Of course, the invention is not limited to the modes of implementation described and it is possible to make variant embodiments without departing from the scope of the invention as defined by the claims. In particular, although in the embodiments described, mention has been made of direct use of the stimulating solution, provision may be made to keep the stimulating solution in lyophilized form. In this case it is possible to act on the concentration of the stimulating solution during the rehydration of the lyophilisate. In particular for soaking the nucleus or the graft, a solution obtained by diluting 50 mg to 150 mg, and preferably 100 mg, of lyophilisate in 50 ml of water is used while for an injection the same amounts of lyophilisate are diluted in 20 ml of water only. Similarly, as regards the search for various pearl colors, although the invention has been described in relation to a recipient oyster of dark color and "stimulating solutions obtained from oysters of light color, provision may be made to invert these colors for example by using a stimulating solution extracted from an oyster of the genus Pinctada species Margaritifera introduced into the gonadal pocket of an oyster of the genus Pinctada species normal Fucata. One can also use oysters of a genus or of a different species from those listed, for example an oyster of the genus Pinctada species Maxima. This gives clear pearls which can have different golden or silvery. Although the description has been made more particularly in relation to the cultivation of pearls in oysters, it is also possible to envisage a cultivation of pearls using other pearly molluscs such as abalone.
Claims
1. Procédé de culture de perles en utilisant un mollusque nacrier receveur ayant une poche gonadique, le procédé comportant l'étape d'introduire un nucleus dans la poche gonadique du mollusque nacrier, caractérisé en ce qu'il comporte en outre l'étape d'introduire dans la poche gonadique une solution stimulante de protéines et de molécules associées d'origine nacrière. 1. A method of culturing pearls using a receiving pearl mollusc having a gonadal pocket, the method comprising the step of introducing a nucleus into the gonadal pocket of the pearly mollusc, characterized in that it further comprises step d '' Introduce into the gonadal pocket a stimulating solution of proteins and associated molecules of pearly origin.
2. Procédé selon la revendication 1, caractérisé en ce que l'introduction de solution stimulante dans la poche gonadique est effectuée en trempant le nucleus dans la solution stimulante préalablement à son introduction dans la poche gonadique. 2. Method according to claim 1, characterized in that the introduction of stimulating solution into the gonadal pocket is carried out by soaking the nucleus in the stimulating solution before its introduction into the gonadal pocket.
3. Procédé selon la revendication 1, caractérisé en ce qu'il comporte en outre l'étape d'introduire un greffon dans la poche gonadique et en ce que l'introduction de la solution stimulante dans la poche gonadique est effectuée en trempant le greffon dans la solution stimulante préalablement à son introduction dans la poche gonadique. 3. Method according to claim 1, characterized in that it further comprises the step of introducing a graft into the gonadal pocket and in that the introduction of the stimulating solution into the gonadal pocket is carried out by soaking the graft in the stimulating solution prior to its introduction into the gonadal pocket.
4. Procédé selon la revendication 1, caractérisé en ce que la solution stimulante est injectée dans la poche gonadique . - 5. Procédé* selon la revendication 1, caractérisé en ce que la solution stimulante est obtenue en réduisant en poudre de la nacre selon une granulométrie inférieure à 50 μ et en mettant la poudre obtenue en contact avec de l'eau ultrapure à raison de 25 g à 100 g de poudre pour 100 ml d'eau, suivie d'une extraction de la phase aqueuse . 6. Procédé selon la revendication 5, caractérisé en ce que la phase aqueuse est lyophilisée et en ce qu'une solution stimulante est reconstituée en mélangeant 50 g à 150 mg, de préférence 100 mg dans 20 ml à 50 ml d'eau stérile en fonction du mode de mise en œuvre de la solution stimulante. 7. Procédé selon la revendication 1, caractérisé en ce que la solution stimulante est réalisée à partir d'une nacre ayant une couleur sélectionnée. 8. Procédé selon la revendication 7, caractérisé en ce que la solution stimulante est extraite de la nacre d'une huître du genre Pinctada espèce Margaritifera albi- nos et le mollusque nacrier receveur est une huître du genre Pinctada espèce Margaritifera normale. 9. Procédé selon la revendication 7, caractérisé en ce que la solution stimulante est extraite de la nacre d'une huître du genre Pinctada espèce Maculata normale et le mollusque récepteur est une huître du genre Pinctada espèce Margaritifera normale. 10. Procédé selon la revendication 7, caractérisé en ce que la solution stimulante est extraite de la nacre d'une huître du genre Pinctada espèce Margaritifera nor- maie et le mollusque nacrier receveur est une huître du genre Pinctada espèce Fucata normale: 11. Procédé selon la revendication 7, caractérisé en ce que la solution stimulante est extraite de la nacre d'une huître du genre Pinctada espèce Maculata ou Marga- ritifera. et le mollusque nacrier receveur est une huître du genre Pinctada espèce Maxima. 12. Procédé selon la revendication 7, caractérisé en ce que la solution stimulante est extraite de la nacre d'une huître du genre Pinctada espèce Maxima et le mol- lusque nacrier receveur est une huître du genre Pinctada espèce Margaritifera. 4. Method according to claim 1, characterized in that the stimulating solution is injected into the gonadal pocket. - 5. Method * according to claim 1, characterized in that the stimulating solution is obtained by reducing mother-of-pearl powder to a particle size of less than 50 μ and putting the powder obtained in contact with ultrapure water at a rate of 25 g to 100 g of powder for 100 ml of water, followed by extraction of the aqueous phase. 6. Method according to claim 5, characterized in that the aqueous phase is lyophilized and in that a stimulating solution is reconstituted by mixing 50 g to 150 mg, preferably 100 mg in 20 ml to 50 ml of sterile water depending on the mode of implementation of the stimulating solution. 7. Method according to claim 1, characterized in that the stimulating solution is produced from a mother-of-pearl having a selected color. 8. Method according to claim 7, characterized in that the stimulating solution is extracted from the mother-of-pearl of an oyster of the genus Pinctada species Margaritifera albi- nos and the receiving pearl mollusk is an oyster of the genus Pinctada species Margaritifera normal. 9. Method according to claim 7, characterized in that the stimulating solution is extracted from the nacre of an oyster of the genus Pinctada normal Maculata species and the receiving mollusc is an oyster of the genus Pinctada normal Margaritifera species. 10. Method according to claim 7, characterized in that the stimulating solution is extracted from the mother-of-pearl of an oyster of the genus Pinctada species Margaritifera noria and the receiving pearl mollusk is an oyster of the genus Pinctada species normal Fucata: 11. Process according to claim 7, characterized in that the stimulating solution is extracted from the mother-of-pearl of an oyster of the genus Pinctada species Maculata or Margaritiveera. and the receiving pearl mollusk is an oyster of the genus Pinctada species Maxima. 12. Method according to claim 7, characterized in that the stimulating solution is extracted from the mother-of-pearl of an oyster of the genus Pinctada Maxima species and the receiving pearly shellfish is an oyster of the genus Pinctada species Margaritifera.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/FR2003/003520 WO2005063005A1 (en) | 2003-11-28 | 2003-11-28 | Pearl culture method stimulated by proteins of mother-of-pearl origin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/FR2003/003520 WO2005063005A1 (en) | 2003-11-28 | 2003-11-28 | Pearl culture method stimulated by proteins of mother-of-pearl origin |
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| WO2005063005A1 true WO2005063005A1 (en) | 2005-07-14 |
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| PCT/FR2003/003520 WO2005063005A1 (en) | 2003-11-28 | 2003-11-28 | Pearl culture method stimulated by proteins of mother-of-pearl origin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2889793A1 (en) * | 2005-08-17 | 2007-02-23 | Robert Wan Holding | Improving the survival of oyster larvae during their fixing in which the larvae is nourished with a nutritive solution added to the breeding water from the hatching of larvae until fixing |
| WO2008012065A1 (en) * | 2006-07-25 | 2008-01-31 | Poemata Raapoto | Method of manufacturing a mabe pearl |
| FR2909287A1 (en) * | 2006-11-07 | 2008-06-06 | Raapoto Poemata Solange Uriri | Introducing finely carved stone being fine and patterned and meeting rigorous rules of height of relief and trough depth, in mollusk shell, useful to obtain pearl layer and graft the beads |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0556729A (en) * | 1991-08-31 | 1993-03-09 | Taiyo Fishery Co Ltd | Method for promoting growth of pearl |
| JPH10108582A (en) * | 1996-10-08 | 1998-04-28 | Tazaki Shinjiyu Kk | Nucleus insertion method for cultured pearl |
| WO2004016078A1 (en) * | 2002-08-12 | 2004-02-26 | Robert Wan Holding | Method for pearl culture stimulated by proteins of mother-of-pearl origin |
-
2003
- 2003-11-28 WO PCT/FR2003/003520 patent/WO2005063005A1/en unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0556729A (en) * | 1991-08-31 | 1993-03-09 | Taiyo Fishery Co Ltd | Method for promoting growth of pearl |
| JPH10108582A (en) * | 1996-10-08 | 1998-04-28 | Tazaki Shinjiyu Kk | Nucleus insertion method for cultured pearl |
| WO2004016078A1 (en) * | 2002-08-12 | 2004-02-26 | Robert Wan Holding | Method for pearl culture stimulated by proteins of mother-of-pearl origin |
Non-Patent Citations (2)
| Title |
|---|
| PATENT ABSTRACTS OF JAPAN vol. 017, no. 364 (C - 1081) 9 July 1993 (1993-07-09) * |
| PATENT ABSTRACTS OF JAPAN vol. 1998, no. 09 31 July 1998 (1998-07-31) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2889793A1 (en) * | 2005-08-17 | 2007-02-23 | Robert Wan Holding | Improving the survival of oyster larvae during their fixing in which the larvae is nourished with a nutritive solution added to the breeding water from the hatching of larvae until fixing |
| WO2008012065A1 (en) * | 2006-07-25 | 2008-01-31 | Poemata Raapoto | Method of manufacturing a mabe pearl |
| FR2904188A1 (en) * | 2006-07-25 | 2008-02-01 | Raapoto Poemata Solange | METHOD FOR MOLLUSCULUS TO PRODUCE A NACRIERE LAYER ON NUCLEUS WITH FINELY SCULPTED RELIEF TO OBTAIN NACRIERE PROTUBERANCE IN RESPECT OF PROPORTIONS |
| FR2909287A1 (en) * | 2006-11-07 | 2008-06-06 | Raapoto Poemata Solange Uriri | Introducing finely carved stone being fine and patterned and meeting rigorous rules of height of relief and trough depth, in mollusk shell, useful to obtain pearl layer and graft the beads |
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