JPH0556729A - Method for promoting growth of pearl - Google Patents

Abstract

PURPOSE:To easily obtain a pearl having large diameter and high quality by administering a mother-of-pearl with a factor to promote the growth of pearl, thereby promoting the secretion of nacre in the culture of pearls. CONSTITUTION:A factor effective for promoting the growth of pearl, e.g. tuna growth hormone extracted and purified from the pituitary gland of tuna and an insulin-like growth factor obtained from blood of mammal is administered to a mother-of-pearl. The administration is carried out either by immersing a piece in the solution of the above factor and inserting the piece into the mother-of-pearl or by injecting the factor into the genital gland of the shellfish.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a method for promoting pearl growth.

[0002]

2. Description of the Related Art Pearl culture is the formation of a pearl layer that surrounds the nucleus by causing a small round nucleus made from the shell of the pearl oyster to enter the body of the pearl oyster and causing a pathological phenomenon in the body of the pearl oyster. The goal is to make pearls, a natural gem that has the brilliance and thickness formed by calcium carbonate, which is the main component of secretions, and a protein called conchiolin, which accounts for about 5% of the accessory components, and water.

A piece of mantle of the pearl oyster is called a piece,
To put it in more detail, firstly 2-3
A piece is cut into a strip with a width of mm and further cut into a length of 2 to 3 mm to make a square. 2 pieces of this piece
Insert into the reproductive line of the mother shell, sandwiching the two nuclei. A large number of activators that improve the function of this piece (colchicine, ribonucleic acid, protein enzyme, inosinic acid, etc.) have already been marketed, but none have an excellent effect. The piece encases the nucleus while growing by its epithelium and connective tissue, and at the same time thickens the pearl. Therefore, the use of the above-mentioned activator was aimed not at promoting the growth of the whole pearl oyster but rather at promoting the division of the peace cells themselves, cell expansion, and increase in the amount of secretion.

[0004]

All of the conventional techniques increase the size or weight of pearls, and the effect on the quality determined by pearl luster and color has not been improved. Moreover, since the step of inserting the nucleus has been established, it was difficult to incorporate the activator into the complicated piece into the step of inserting the nucleus. Therefore, it is a problem to be solved by the present invention to find out a factor that improves the size and quality of pearls, introduce the factor into a mother oyster by a simple method, and further incorporate it into the intubation step.

[0005]

[Means for Solving the Problems] Means for solving the above problems, that is, the present invention is a pearl growth promoting method characterized by administering a factor for promoting pearl growth to a mother oyster. A pearl growth promoting method, wherein the growth promoting factor is a growth hormone or a growth promoting factor, and more specifically, tuna growth hormone extracted from the pituitary gland of tuna growth hormone or a recombinant tuna growth hormone thereof. (R-tGH), and the growth promoting factor is insulin-like growth factor (IGF) obtained from mammalian blood, which is a pearl growth promoting method.

Further, in the present invention, the method of administering a factor for promoting pearl growth to a mother oyster is to insert a piece immersed in a solution of the factor for promoting pearl growth into the mother oyster, or pearl growing It is a method of promoting pearl growth, which is to inject a factor that promotes pearl growth into the gonad of the mother oyster. Next, this method will be specifically described.

[0007] When inserting a nucleus of 6.9 mm into a 2-year-old shellfish having an average weight of about 76 g, an average shell height of about 72.3 mm, and an average shell width of about 29.6 mm, a tuna brain is placed in the gonad of one individual. Tuna growth hormone extracted and purified from the pituitary gland (JP-A-63-133951)
No.) and tuna growth hormone gene are isolated and integrated into a plasmid and expressed in E. coli (JP-A-1-104180),
Regenerated into an active form (JP-A-2-223596) Tuna growth hormone (r-tGH) or Rin-derkneckt E. and Humbel,
Prepared according to the method of RE (J. Biol. Chem., 253, 276
9-2776 (1978)) Insulin-like growth factor (IGF) 1mg ~
It is carried out by injecting 1 ng / 2 μl seawater into the gonad of the mother oyster. IGF is a peptide with a molecular weight of about 7,500, and its actions are (a) cartilage growth in mammals, (b) promotion of chondroitin sulfate synthesis in cartilage, and (c) hypertrophy of cell growth in liver and other organs, amino acid transport, It is known to promote protein synthesis. Prior to inserting the same size nucleus into the same size mother oyster, insert the piece into the mother oyster soaked for 30 minutes in r-tGH or IGF solution prepared in a concentration of 1 to 800 ppm with piece seawater. By doing things.

[0008]

[Effects of the Invention] The beads removed after peeling are classified into grades 1 and 2 in order of increasing value. Further, within each grade, the beads are divided into 7 mm, 8 mm, and 9 mm according to the bead diameter. The total weight of each pearl diameter section is determined by administering r-tGH or IGF to the gonad, or immersing the piece in r-tGH or IGF to promote secretion of the nacre and increase the pearl diameter. ,
Good quality beads can be produced.

[0009]

【Example】

[0010]

Example 1 A two-year-old shellfish produced in 1987 having an average body weight of about 76 g, an average shell height of about 72.3 mm and an average shell width of about 29.6 mm was used as a mother shellfish. The procaryotic nucleus used had a size of 6.9 mm.
30 to 60 minutes before transection, 1 mg of r-tGH in the gonad of one individual
1 ng / 2 μl seawater was injected. As a control group, a group inserted by the usual nucleus insertion method was used. Each group had 8000 individuals, and after growing for 2 years by a normal breeding method, they were stripped. The quality and diameter of the peeled beads are summarized in the table below. Grade 1 and grade 2 in descending order of quality, and the diameter of the beads was divided into 7 mm, 8 mm, and 9 mm. The total weight of each beads of each grade was shown.

[0011]

[Table 1]

From Table 1, by injecting r-tGH,
It can be seen that the weights of the first and second grade total beads increased as compared to the control group.

[0013]

Example 2 A 2-year-old shellfish having an average body weight of about 76 g, an average shell height of about 72.3 mm and an average shell width of about 29.6 mm was used as a mother clam. The procaryotic nucleus used had a size of 6.9 mm.
Prepared to a concentration of 1-800 ppm with seawater 30-60 minutes before intubation
The piece immersed in the r-tGH solution for 30 minutes was inserted into the mother oyster.
As a control group, a group inserted by the usual nucleus insertion method was used. Each group had 8000 individuals, and after growing for 2 years by a normal breeding method, they were stripped. The quality and diameter of the peeled beads are summarized in the table below. Grade 1 and grade 2 in order of good quality, with a pearl diameter of 7 mm, 8
mm and 9 mm. The total weight of each diameter category of each grade is shown.

[0014]

[Table 2]

From these results, it can be seen that the use of the pieces dipped in r-tGH increased the total weight of the first and second grade beads as compared with the control group.

[0016]

[Example 3] A 2-year-old shellfish having an average body weight of about 76 g, an average shell height of about 72.3 mm and an average shell width of about 29.6 mm was used as a mother clam. The procaryotic nucleus used had a size of 6.9 mm.
30 to 60 minutes before transection, 1 mg to 1 mg of IGF in the gonad of one individual
ng / 2 μl seawater was injected. As a control group, a group inserted by the usual nucleus insertion method was used. Each group has 800 individuals,
After being grown for 2 years by the usual breeding method, they were stripped. The quality and diameter of the peeled beads are summarized in the table below. Grade 1 and 2 in order of good quality
The beads were divided into 7 mm, 8 mm and 9 mm diameters. The total weight of each diameter category of each grade was shown.

[0017]

[Table 3]

It can be seen from Table 3 that the injection of IGF increased the total weight of the first-class and second-class beads as compared with the control group.

[0019]

Example 4 A 2-year-old shellfish having an average weight of about 76 g, an average shell height of about 72.3 mm and an average shell width of about 29.6 mm was used as a mother shellfish in 1987. The procaryotic nucleus used had a size of 6.9 mm.
Before the core insertion, a piece dipped in an IGF solution adjusted to a concentration of 1 to 800 ppm with seawater for 30 minutes was inserted into a mother oyster. As a control group, a group inserted by the usual nucleus insertion method was used. Each group consisted of 8000 individuals, and after growing for 2 years by a normal breeding method, they were stripped. The quality and diameter of the peeled beads are summarized in the table below. Grade 1 and grade 2 in descending order of quality, and the diameter of the beads was divided into 7 mm, 8 mm, and 9 mm. The total weight of each diameter category of each grade is shown.

[0020]

[Table 4]

From Table 4, by using the pieces dipped in the IGF solution, the grades 1 and 2 were compared with the control group.
It can be seen that the total pearl weight of the class has increased.

 ─────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Shoji Kimura 16-2 Wadai, Tsukuba-shi, Ibaraki Taiyo Fisheries Co., Ltd. Central Research Institute

Claims (6)

[Claims] 1. A method for promoting pearl growth, which comprises administering a factor that promotes pearl growth to a mother oyster. 2. The method according to claim 1, wherein the factor that promotes pearl growth is growth hormone or a growth promoting factor. 3. The method according to claim 2, wherein the growth hormone is tuna growth hormone extracted and purified from tuna pituitary gland or recombinant tuna growth hormone (r-tGH) thereof. 4. The method of claim 2, wherein the growth promoting factor is insulin-like growth factor (IGF) obtained from mammalian blood. 5. The method according to any one of claims 1 to 4, wherein the method of administering the mother oyster is to insert a piece immersed in a solution of a factor that promotes pearl growth into the mother pearl oyster. 6. The method according to any one of claims 1 to 4, wherein the method of administering the mother oyster is to inject a factor that promotes pearl growth into the gonad of the mother pearl oyster.