FR2843277A1 - Production of cultured pearls comprises introducing a solution of proteins and associated molecules derived from mother-of-pearl into the gonadal pouch of a mollusc - Google Patents
Production of cultured pearls comprises introducing a solution of proteins and associated molecules derived from mother-of-pearl into the gonadal pouch of a mollusc Download PDFInfo
- Publication number
- FR2843277A1 FR2843277A1 FR0210213A FR0210213A FR2843277A1 FR 2843277 A1 FR2843277 A1 FR 2843277A1 FR 0210213 A FR0210213 A FR 0210213A FR 0210213 A FR0210213 A FR 0210213A FR 2843277 A1 FR2843277 A1 FR 2843277A1
- Authority
- FR
- France
- Prior art keywords
- pearl
- oyster
- solution
- species
- gonadal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000011049 pearl Substances 0.000 title claims abstract description 39
- 230000002710 gonadal effect Effects 0.000 title claims abstract description 17
- 241000237852 Mollusca Species 0.000 title claims abstract description 12
- 102000004169 proteins and genes Human genes 0.000 title abstract description 7
- 108090000623 proteins and genes Proteins 0.000 title abstract description 7
- 239000010984 cultured pearl Substances 0.000 title abstract 2
- 239000000243 solution Substances 0.000 claims description 34
- 241000237502 Ostreidae Species 0.000 claims description 33
- 235000020636 oyster Nutrition 0.000 claims description 33
- 230000004936 stimulating effect Effects 0.000 claims description 32
- 238000000034 method Methods 0.000 claims description 18
- 241000490567 Pinctada Species 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 10
- 241000217407 Margaritifera Species 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 210000002149 gonad Anatomy 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 2
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 239000008346 aqueous phase Substances 0.000 claims 2
- 241001464019 Pinctada margaritifera Species 0.000 claims 1
- 241001490476 Pinctada maxima Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 239000012460 protein solution Substances 0.000 claims 1
- 239000008223 sterile water Substances 0.000 claims 1
- 239000003086 colorant Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 238000001033 granulometry Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
Description
La présente invention concerne un procédé de culture de perles.The present invention relates to a pearl cultivation method.
On sait que pour réaliser la culture de perles on utilise un mollusque nacrier receveur, généralement une 5 huître receveuse, ayant une poche gonadique dans laquelle est introduit un nucleus. Lors d'une greffe originale, c'est-à-dire lorsque l'huître receveuse reçoit un nucleus pour la première fois, on ajoute en outre un greffon sous forme d'un petit morceau de manteau d'une huître don10 neuse. Lors d'une regreffe, c'est-àdire lorsque l'huître It is known that to carry out the cultivation of pearls a receiving pearl mollusc is used, generally a recipient oyster, having a gonadal pocket into which a nucleus is introduced. During an original transplant, that is to say when the recipient oyster receives a nucleus for the first time, a graft is also added in the form of a small piece of mantle from a donor oyster. During a transplant, i.e. when the oyster
receveuse a déjà produit une première perle, un second nucleus est introduit dans la poche gonadique de l'huître receveuse et il n'est généralement pas nécessaire de remettre un greffon. recipient has already produced a first pearl, a second nucleus is introduced into the gonad pocket of the recipient oyster and it is generally not necessary to deliver a graft.
Qu'il s'agisse d'une greffe originale ou d'une Whether it's an original transplant or a
regreffe, un dépôt significatif de nacre sur le nucleus n'est généralement obtenu qu'après un peu plus d'un an à partir de l'introduction du nucleus et pour dbtenir une perle de qualité il est généralement nécessaire d'atten20 dre au moins deux ans. regreffe, a significant deposit of mother-of-pearl on the nucleus is generally obtained only after a little more than a year from the introduction of the nucleus and to obtain a quality pearl it is generally necessary to wait at least two years.
Un but de l'invention est de proposer un procédé An object of the invention is to propose a method
permettant d'accélérer la formation et améliorer la qualité du dépôt de nacre sur le nucleus. allowing to accelerate the formation and to improve the quality of the nacre deposit on the nucleus.
Selon l'invention, on propose un procédé compor25 tant l'étape d'introduire dans la poche gonadique une solution stimulante de protéines et de molécules associées According to the invention, a method is proposed comprising both the step of introducing into the gonadal pocket a stimulating solution of proteins and associated molecules.
d'origine nacrière.of pearl origin.
En ajoutant cette étape au procédé de greffe traditionnel, qu'il s'agisse d'une greffe originale ou d'une 30 regreffe, on a constaté une accélération remarquable dans By adding this step to the traditional grafting process, whether it is an original graft or a re-graft, there has been a remarkable acceleration in
la formation du dépôt de nacre dans la mesure o un dépôt significatif de nacre a été obtenu quelques mois seulement après l'introduction du nucleus dans la poche gonadique de l'huître receveuse. the formation of the nacre deposit insofar as a significant nacre deposit was obtained only a few months after the introduction of the nucleus into the gonadal pocket of the recipient oyster.
Pour mettre en oeuvre le procédé selon l'invention, il convient tout d'abord de réaliser la solution stimulante contenant les protéines et les molécules associées d'origine nacrière. Dans le cas d'une cul5 ture de perles en utilisant une huître, on réalise tout d'abord une plaque de nacre par ponçage des couches extérieures d'une coquille d'huître jusqu'à l'apparition de la couleur irisée de la nacre. On effectue ensuite une réduction en poudre, par exemple par sciage des plaques 10 de nacre décrotées et broyage, et on sélectionne de préférence la fraction de poudre de granulométrie inférieure à 50,m. La poudre de nacre ainsi obtenue est mise en contact avec de l'eau ultrapure ou apirogène par trempage de la poudre dans l'eau ultrapure, à raison de 25 g à 100 15 g de poudre pour 100 ml d'eau en fonction de la granulométrie de la poudre, en agitant le mélange de façon continue pendant environ vingt heures. Le mélange obtenu est centrifugé puis filtré pour séparer la partie solide de la partie liquide et ne conserver que la partie liquide 20 qui forme la solution stimulante contenant les protéines To implement the method according to the invention, it is first of all necessary to produce the stimulating solution containing the proteins and associated molecules of pearly origin. In the case of a pearl culture using an oyster, a mother-of-pearl plate is first produced by sanding the outer layers of an oyster shell until the iridescent color of the mother-of-pearl appears . A powder reduction is then carried out, for example by sawing off the disconnected mother-of-pearl plates 10 and grinding, and the fraction of powder with a particle size of less than 50 μm is preferably selected. The mother-of-pearl powder thus obtained is brought into contact with ultrapure or aspirogenic water by soaking the powder in ultrapure water, at a rate of 25 g to 100 15 g of powder per 100 ml of water depending on the granulometry of the powder, stirring the mixture continuously for about twenty hours. The mixture obtained is centrifuged then filtered to separate the solid part from the liquid part and keep only the liquid part 20 which forms the stimulating solution containing the proteins.
et les molécules associées.and associated molecules.
Le procédé selon l'invention peut alors être mis en oeuvre de différentes façons. Selon un premier mode de mise en oeuvre préféré, le nucleus est trempé dans la so25 lution stimulante préalablement à son introduction dans The method according to the invention can then be implemented in different ways. According to a first preferred embodiment, the nucleus is soaked in the stimulating solution prior to its introduction into
la poche gonadique de l'huître perlière. the gonadal pocket of the pearl oyster.
Selon un second mode de mise en oeuvre réalisable lors d'une greffe originale, le greffon est trempé dans la solution stimulante préalablement à son introduction 30 dans la poche gonadique. On notera que dans ce cas il est According to a second mode of implementation achievable during an original graft, the graft is soaked in the stimulating solution prior to its introduction into the gonadal pocket. Note that in this case it is
également possible de tremper à la fois le nucleus et le greffon dans la solution stimulante avant leur introduction dans la poche gonadique. it is also possible to soak both the nucleus and the graft in the stimulating solution before their introduction into the gonadal pocket.
Selon un troisième mode de mise en oeuvre, une in- According to a third embodiment, an information
jection de solution stimulante est effectuée dans la poche gonadique avant ou après introduction du nucleus. Stimulating solution is injected into the gonadal pocket before or after introduction of the nucleus.
Comme dans le cas précédent une injection de solution stimulante peut être réalisé conjointement à un recouvre5 ment du nucleus et/ou du greffon avec la solution stimulante. As in the previous case, an injection of stimulating solution can be carried out in conjunction with a covering of the nucleus and / or of the graft with the stimulating solution.
Selon un autre aspect de l'invention, la solution stimulante est en outre utilisée pour faire varier la couleur de la perle obtenue. En effet, dans le mode de 10 mise en oeuvre décrit ci-dessus pour l'obtention de la solution stimulante, on remarquera que l'intégralité de la plaque de nacre d'une huître est utilisée pour réaliser la poudre de nacre dont sont extraites les protéines et les molécules associées. Ceci signifie que des protéines 15 vont non seulement être extraites de la partie blanche de la nacre mais également de la partie colorée qui est représentative du genre et de l'espèce de l'huître dont la According to another aspect of the invention, the stimulating solution is also used to vary the color of the pearl obtained. Indeed, in the embodiment described above for obtaining the stimulating solution, it will be noted that the entire mother-of-pearl plate of an oyster is used to make the mother-of-pearl powder from which are extracted proteins and associated molecules. This means that proteins will not only be extracted from the white part of the mother-of-pearl but also from the colored part which is representative of the genus and species of the oyster whose
nacre est extraite.mother-of-pearl is extracted.
Pour obtenir des perles de différentes couleurs à 20 partir d'une même huître donneuse, ou d'une façon plus générale à partir d'un même mollusque nacrier, on peut réaliser la solution stimulante à partir d'une nacre de couleur sélectionnée, soit en utilisant une zone particulière de la coquille, soit en utilisant de la nacre pro25 venant d'une huître ayant une couleur différente de celle To obtain pearls of different colors from the same donor oyster, or more generally from the same mother-of-pearl mollusc, the stimulant solution can be produced from a mother-of-pearl of selected color, either using a particular area of the shell, or using pro25 mother-of-pearl from an oyster having a color different from that
l'huître receveuse.the recipient oyster.
A titre d'exemple, une solution stimulante réalisée à partir d'une huître du genre Pinctada, espèce Margaritifera variant albinos, utilisée avec une huître re30 ceveuse du genre Pinctada espèce Margaritifera normale permet d'obtenir une perle présentant un orient gris argenté très doux avec des reflets de couleurs pastels type For example, a stimulating solution made from an oyster of the genus Pinctada, species Margaritifera variant albino, used with an oyster of the genus Pinctada species Margaritifera normal makes it possible to obtain a pearl having a very silvery gray orient with typical pastel color reflections
gorge de pigeon.pigeon throat.
De même, avec une solution stimulante réalisée à partir de la nacre d'une huître du genre Pinctada espèce Maculata et utilisée en relation avec une huître receveuse du genre Pinctada espèce Margaritifera normale, la perle obtenue est une perle dorée à reflets sombres ayant un orient très chatoyant. Bien entendu, l'invention n'est pas limitée aux Similarly, with a stimulating solution made from the nacre of an oyster of the genus Pinctada species Maculata and used in conjunction with a recipient oyster of the genus Pinctada species Margaritifera normal, the pearl obtained is a golden pearl with dark reflections having an orient very shimmering. Of course, the invention is not limited to
modes de mise en oeuvre décrits et on peut y apporter des variantes de réalisation sans sortir du cadre de l'invention tel que défini par les revendications. Modes of implementation described and it is possible to add variants without departing from the scope of the invention as defined by the claims.
En particulier, bien que dans les modes de mise en oeuvre décrits il ait été fait mention d'une utilisation directe de la solution stimulante, on peut prévoir de conserver la solution stimulante sous forme lyophilisée. Dans ce cas il est possible d'agir sur la concentra15 tion de la solution stimulante lors de la réhydratation du lyophilisat. En particulier pour un trempage du nucleus ou du greffon, on utilise une solution obtenue en diluant 50 mg à 150 mg, et de préférence 100 mg, de lyophilisat dans 50 ml d'eau tandis que pour une injection 20 les mêmes quantités de lyophilisat sont diluées dans 20 In particular, although in the embodiments described, mention has been made of direct use of the stimulating solution, provision may be made to keep the stimulating solution in lyophilized form. In this case it is possible to act on the concentration of the stimulating solution during the rehydration of the lyophilisate. In particular for soaking the nucleus or the graft, use is made of a solution obtained by diluting 50 mg to 150 mg, and preferably 100 mg, of lyophilisate in 50 ml of water while for an injection the same amounts of lyophilisate are diluted in 20
ml d'eau seulement.ml of water only.
De même, en ce qui concerne la recherche de couleurs de perles variées, bien que l'invention ait été décrite en relation avec une huître receveuse de couleur 25 sombre et des solutions stimulantes obtenues à partir d'huîtres de couleur claire, on peut prévoir d'inverser ces couleurs par exemple en utilisant une solution stimulante extraite d'une huître du genre Pinctada espèce Margaritifera introduite dans la poche gonadique d'une huî30 tre du genre Pinctada espèce Fucata normale. On peut également utiliser des huîtres d'un genre ou d'une espèce différente de ceux énoncés, par exemple une huître du genre Pinctada espèce Maxima. On obtient ainsi des perles claires pouvant présenter différentes nuances dorées ou argentées. Likewise, with regard to the search for various pearl colors, although the invention has been described in relation to a recipient oyster of dark color and stimulating solutions obtained from light oysters, provision may be made invert these colors for example by using a stimulating solution extracted from an oyster of the genus Pinctada species Margaritifera introduced into the gonad pocket of an oyster of the genus Pinctada species normal Fucata. One can also use oysters of a genus or species different from those listed, for example an oyster of the genus Pinctada species Maxima. Clear pearls are thus obtained, which can have different shades of gold or silver.
Bien que la description ait été faite plus particulièrement en relation avec la culture de perles dans les huîtres, on peut également envisager une culture de 5 perles utilisant d'autres mollusques nacriers tels que Although the description has been made more particularly in relation to the cultivation of pearls in oysters, it is also possible to envisage a culture of 5 pearls using other pearly molluscs such as
des ormeaux.abalone.
Claims (12)
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0210213A FR2843277B1 (en) | 2002-08-12 | 2002-08-12 | PROCESS FOR THE CULTURE OF PEARLS STIMULATED BY PROTEINS OF NACRIOR ORIGIN |
AU2003284974A AU2003284974B2 (en) | 2002-08-12 | 2003-07-10 | Method for pearl culture stimulated by proteins of mother-of-pearl origin |
NZ538022A NZ538022A (en) | 2002-08-12 | 2003-07-10 | A method of culturing pearls stimulated by nacre-originating proteins |
PCT/FR2003/002172 WO2004016078A1 (en) | 2002-08-12 | 2003-07-10 | Method for pearl culture stimulated by proteins of mother-of-pearl origin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0210213A FR2843277B1 (en) | 2002-08-12 | 2002-08-12 | PROCESS FOR THE CULTURE OF PEARLS STIMULATED BY PROTEINS OF NACRIOR ORIGIN |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2843277A1 true FR2843277A1 (en) | 2004-02-13 |
FR2843277B1 FR2843277B1 (en) | 2004-10-22 |
Family
ID=30471073
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR0210213A Expired - Fee Related FR2843277B1 (en) | 2002-08-12 | 2002-08-12 | PROCESS FOR THE CULTURE OF PEARLS STIMULATED BY PROTEINS OF NACRIOR ORIGIN |
Country Status (4)
Country | Link |
---|---|
AU (1) | AU2003284974B2 (en) |
FR (1) | FR2843277B1 (en) |
NZ (1) | NZ538022A (en) |
WO (1) | WO2004016078A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2866201A1 (en) * | 2004-02-13 | 2005-08-19 | Robert Wan Holding | Cultivating pearls comprises introducing nucleus into gonad pocket of recipient nacreous mollusk and introducing stimulating product into gonad pocket of recipient nacreous mollusk based on extrapalleal fluid, during growth of pearl |
FR2889793A1 (en) * | 2005-08-17 | 2007-02-23 | Robert Wan Holding | Improving the survival of oyster larvae during their fixing in which the larvae is nourished with a nutritive solution added to the breeding water from the hatching of larvae until fixing |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005063005A1 (en) * | 2003-11-28 | 2005-07-14 | Robert Wan Holding | Pearl culture method stimulated by proteins of mother-of-pearl origin |
CN103975883B (en) * | 2014-05-23 | 2016-01-20 | 广东海洋大学 | The selection of the black shell strain of a kind of pteria martensii for cultivating sea water pearls |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0556729A (en) * | 1991-08-31 | 1993-03-09 | Taiyo Fishery Co Ltd | Method for promoting growth of pearl |
JPH10108582A (en) * | 1996-10-08 | 1998-04-28 | Tazaki Shinjiyu Kk | Nucleus insertion method for cultured pearl |
-
2002
- 2002-08-12 FR FR0210213A patent/FR2843277B1/en not_active Expired - Fee Related
-
2003
- 2003-07-10 AU AU2003284974A patent/AU2003284974B2/en not_active Ceased
- 2003-07-10 WO PCT/FR2003/002172 patent/WO2004016078A1/en active IP Right Grant
- 2003-07-10 NZ NZ538022A patent/NZ538022A/en unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0556729A (en) * | 1991-08-31 | 1993-03-09 | Taiyo Fishery Co Ltd | Method for promoting growth of pearl |
JPH10108582A (en) * | 1996-10-08 | 1998-04-28 | Tazaki Shinjiyu Kk | Nucleus insertion method for cultured pearl |
Non-Patent Citations (2)
Title |
---|
PATENT ABSTRACTS OF JAPAN vol. 017, no. 364 (C - 1081) 9 July 1993 (1993-07-09) * |
PATENT ABSTRACTS OF JAPAN vol. 1998, no. 09 31 July 1998 (1998-07-31) * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2866201A1 (en) * | 2004-02-13 | 2005-08-19 | Robert Wan Holding | Cultivating pearls comprises introducing nucleus into gonad pocket of recipient nacreous mollusk and introducing stimulating product into gonad pocket of recipient nacreous mollusk based on extrapalleal fluid, during growth of pearl |
WO2005077161A2 (en) * | 2004-02-13 | 2005-08-25 | Robert Wan Holding | Method for cultivating peals stimulated by extrapalleal liquid |
WO2005077161A3 (en) * | 2004-02-13 | 2005-11-10 | Robert Wan Holding | Method for cultivating peals stimulated by extrapalleal liquid |
FR2889793A1 (en) * | 2005-08-17 | 2007-02-23 | Robert Wan Holding | Improving the survival of oyster larvae during their fixing in which the larvae is nourished with a nutritive solution added to the breeding water from the hatching of larvae until fixing |
Also Published As
Publication number | Publication date |
---|---|
AU2003284974B2 (en) | 2007-06-14 |
WO2004016078A1 (en) | 2004-02-26 |
FR2843277B1 (en) | 2004-10-22 |
NZ538022A (en) | 2007-02-23 |
AU2003284974A1 (en) | 2004-03-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP3193821B1 (en) | Method for producing a stable precipitate enriched in phycobiliproteins | |
US5749319A (en) | Pearl and method for producing same | |
FR2501715A1 (en) | PROCESS FOR CULTURING CELLS REQUIRING FASTENING FOR THE MANUFACTURE OF THERAPEUTIC SUBSTANCES | |
FR2991989A1 (en) | COLORING MATERIALS OF VEGETABLE ORIGIN AND THEIR USE FOR COLORING COMPOSITIONS, ESPECIALLY COSMETIC COMPOSITIONS | |
EP2559342A1 (en) | Method for improving the nutritional value of filter-feeding molluscs, in particular oysters, by storage in the presence of microalgae | |
FR2843277A1 (en) | Production of cultured pearls comprises introducing a solution of proteins and associated molecules derived from mother-of-pearl into the gonadal pouch of a mollusc | |
Kalb et al. | New lichen species from Brazil and Venezuela | |
WO2005063005A1 (en) | Pearl culture method stimulated by proteins of mother-of-pearl origin | |
FR2789399A1 (en) | Preparing extracts from photosynthetic microorganisms, useful in nutritional, cosmetic and therapeutic compositions, comprises lysing cells grown on induction medium | |
Avian | Temperature influence on in vitro reproduction and development of Pelagia noctiluca (Forskål) | |
JPH1156346A (en) | Hematococcus algal body containing astaxanthin and its extract | |
FR2996724A1 (en) | Stimulative composition for producing cultured pearls, has proteins and lipids in respective proportions that are adapted to support growth composition of pearls, and to support shining effect and quality of skin of produced pearl | |
FR2654743A1 (en) | METHOD OF GROWING MASS OF DIATOMEE HASLEA OSTRERIA SIMONSEN | |
WO2003067970A1 (en) | Pearl culture method | |
EP1589942A1 (en) | Method for the extraction of lipids from nacreous molluscs | |
FR2620308A1 (en) | Preparation based on fish eggs, similar to caviar, and method for obtaining it | |
WO2005077161A2 (en) | Method for cultivating peals stimulated by extrapalleal liquid | |
FR3109584A1 (en) | Microcompartment for culturing cnidarian cells | |
FR2610006A1 (en) | PROCESS FOR IN VITRO RECONSTITUTION OF TUMORS | |
Muzii et al. | Calcite deposition during shell repair by the aragonitic gastropod Murex fulvescens | |
Montresor et al. | Reproduction and cyst formation in Scrippsiella precaria (Dinophyceae) | |
JP3224630B2 (en) | How to decolor red fish | |
Delaval | An Experimental Inquiry Into the Cause of the Changes of Colours in Opake and Coloured Bodies: With an Historical Preface Relative to the Parts of Philosophy Therein Examined, and to the Several Arts and Manufactures Dependent on Them | |
RU2244735C2 (en) | Method for preparing vodka | |
RU2257405C1 (en) | Method for producing vodka |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse |
Effective date: 20110502 |
|
RN | Application for restoration | ||
FC | Decision of inpi director general to approve request for restoration |
Effective date: 20111109 |
|
PLFP | Fee payment |
Year of fee payment: 15 |
|
PLFP | Fee payment |
Year of fee payment: 16 |
|
PLFP | Fee payment |
Year of fee payment: 17 |
|
PLFP | Fee payment |
Year of fee payment: 18 |
|
PLFP | Fee payment |
Year of fee payment: 19 |
|
PLFP | Fee payment |
Year of fee payment: 20 |