JPH01132315A - Mushroom cultivation - Google Patents
Mushroom cultivationInfo
- Publication number
- JPH01132315A JPH01132315A JP63177396A JP17739688A JPH01132315A JP H01132315 A JPH01132315 A JP H01132315A JP 63177396 A JP63177396 A JP 63177396A JP 17739688 A JP17739688 A JP 17739688A JP H01132315 A JPH01132315 A JP H01132315A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- glucosamine
- cultivation
- mushrooms
- mushroom
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 25
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 claims abstract description 18
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 claims abstract description 15
- 229960002442 glucosamine Drugs 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims description 7
- 150000003839 salts Chemical class 0.000 claims description 4
- 239000002609 medium Substances 0.000 abstract description 26
- 239000001963 growth medium Substances 0.000 abstract description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract description 5
- 239000000428 dust Substances 0.000 abstract 1
- -1 inorganic acid salt Chemical class 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 240000006499 Flammulina velutipes Species 0.000 description 5
- 235000016640 Flammulina velutipes Nutrition 0.000 description 5
- 240000000599 Lentinula edodes Species 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000178 monomer Substances 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- 235000001715 Lentinula edodes Nutrition 0.000 description 2
- 244000168667 Pholiota nameko Species 0.000 description 2
- 235000014528 Pholiota nameko Nutrition 0.000 description 2
- 240000001462 Pleurotus ostreatus Species 0.000 description 2
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 2
- 241000121220 Tricholoma matsutake Species 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000007952 growth promoter Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000020637 scallop Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 2
- CBOJBBMQJBVCMW-BTVCFUMJSA-N (2r,3r,4s,5r)-2-amino-3,4,5,6-tetrahydroxyhexanal;hydrochloride Chemical compound Cl.O=C[C@H](N)[C@@H](O)[C@H](O)[C@H](O)CO CBOJBBMQJBVCMW-BTVCFUMJSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 240000001080 Grifola frondosa Species 0.000 description 1
- 235000007710 Grifola frondosa Nutrition 0.000 description 1
- 244000232885 Naematoloma sublateritium Species 0.000 description 1
- 235000016009 Naematoloma sublateritium Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000237509 Patinopecten sp. Species 0.000 description 1
- 241000237503 Pectinidae Species 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 229960001911 glucosamine hydrochloride Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明はきのこ類の栽培方法に関するものである。[Detailed description of the invention] (Industrial application field) The present invention relates to a method for cultivating mushrooms.
（従来の技術）
従来シイタケ、ナメコ、ヒラタケ、エノキタケ等のきの
こ類の栽培は、はだ水栽培あるいは鋸屑栽培で行われて
きたが培養基をビンにつめて行う、いわゆるビン栽培か
人工栽培の一種として行われている。このような人工栽
培において、栽培室など施設の利用効率を上げ、生産性
を高めるために、きのこの生長を促進し栽培期間を短縮
する方法の開発が望まれている。(Conventional technology) Mushrooms such as shiitake, nameko, oyster mushroom, and enokitake have traditionally been cultivated using bare water cultivation or sawdust cultivation, but it is now possible to cultivate them by filling the culture medium in a bottle, a type of so-called bottle cultivation or artificial cultivation. It is being done. In such artificial cultivation, in order to increase the utilization efficiency of facilities such as cultivation rooms and increase productivity, it is desired to develop a method to promote mushroom growth and shorten the cultivation period.
（発明が解決しようとする課題）
このような目的を満足するため生育の過程によって温湿
度などを最適にした栽培室を設けて移動するなどの方法
をとっている。しかし、栽培室を分けて作るので施設費
がかさむなどの問題があり、また−時期に集中して移動
を行うため労働負荷が大きくなる。(Problems to be Solved by the Invention) In order to satisfy these objectives, methods such as setting up a cultivation room with temperature and humidity optimized depending on the growth process and moving the cultivation room are used. However, since separate cultivation rooms are created, there are problems such as increased facility costs, and the labor load is increased because the cultivation is concentrated at different times of the year.
一方、培地に添加しうる生長促進剤かあればよい。しか
し、このようなものとしては食用に供するものを対象と
するのて、使用しうる物質も非常に制限され、適当な物
質の開発が望まれている。On the other hand, any growth promoter that can be added to the medium is sufficient. However, since these products are intended for edible use, the materials that can be used are very limited, and the development of suitable materials is desired.
本発明は上記の自然栽培及び人工栽培のいずれにも使用
できる天然物由来で無害のきのこ類生長促進剤を用いた
きのこ類の栽培方法を提供することを目的とする。An object of the present invention is to provide a method for cultivating mushrooms using a harmless mushroom growth promoter derived from natural products that can be used in both natural cultivation and artificial cultivation.
（課題を解決するための手段）
本発明者らはこのようなきのこ類の栽培に関する問題点
を克服するため鋭意研究を重ねた結果、エビやカニのカ
ラを塩酸で処理することによって得られるＤ−グルコサ
ミンを添加して培地を調製し、これにきのこの種菌を接
種、培養することによりきのこの生長促進栽培に成功し
、本発明に到達するに至った。(Means for Solving the Problems) The present inventors have conducted intensive research to overcome the problems related to the cultivation of mushrooms, and as a result, we have found that D. - By preparing a medium by adding glucosamine, inoculating the mushroom inoculum into the medium and culturing it, we succeeded in promoting the growth of mushrooms, leading to the present invention.
すなわち本発明は、グルコサミン又はその塩を含有する
培地を用いてきのこ類を栽培することを特徴とするきの
こ類の栽培方法を提供するものである。That is, the present invention provides a method for cultivating mushrooms, which comprises cultivating mushrooms using a medium containing glucosamine or a salt thereof.
本発明を適用しうる培地には特に制限はなく、液体培地
、木粉（鋸屑）培地てもよく、またほだ木を用いる自然
栽培に適用してもよい。例えば、びんに詰める培地は、
通常、鋸屑と米ヌカを主成分とするものあるいはポテト
エキスにグルコースを添加したものなどがあるが、これ
に制限されず、公知のどのような培地を用いてもよい。There is no particular restriction on the medium to which the present invention can be applied, and it may be a liquid medium, a wood powder (sawdust) medium, or it may be applied to natural cultivation using wood. For example, the culture medium to be bottled is
Usually, there are those whose main ingredients are sawdust and rice bran, or those where glucose is added to potato extract, but the medium is not limited thereto, and any known medium may be used.
このような培地は従来からきのこ類、例えばエノキタケ
、ナメコ、ヒラタケ、シイタケ、クリタケ、ブナシメシ
、タモギタケ、マイタケ、ヤナギマツタケ、マツシュル
ーム、マンネンタケなどの固体培地もしくは液体培地と
して用いられている。とりわけ本発明の栽培方法は食用
きのこ類の栽培に好適である。Such a medium has been conventionally used as a solid or liquid medium for mushrooms, such as enokitake, nameko, oyster mushroom, shiitake, kuritake, bunashimeshi, tamogitake, maitake, willow matsutake, pine mushroom, and stone mushroom. In particular, the cultivation method of the present invention is suitable for cultivation of edible mushrooms.
この培地は適量の水を含有することが必要である。水分
含有量は培養条件などを考慮して適宜定められるか、エ
ノキタケの固体培地では培地全体に対し６０〜６５重量
％が好ましい。This medium needs to contain an appropriate amount of water. The water content may be appropriately determined in consideration of culture conditions, etc., and in the case of a solid medium for enokitake, it is preferably 60 to 65% by weight based on the entire medium.
本発明に用いられるグルコサミンはＤ一体てもＬ一体で
もよいか、Ｄ一体がより好ましい。また、グルコサミン
は、塩酸塩などの無機酸の塩として用いるのが好ましい
。The glucosamine used in the present invention may be a D-monomer or an L-monomer, and is more preferably a D-monomer. Moreover, glucosamine is preferably used as a salt of an inorganic acid such as hydrochloride.
グルコサミンの添加量は例えば人工培地中０．０５重量
％以上が好ましく、０．３〜１．０重量％がより好まし
い。グルコサミンが少なすぎると子実体の掘生が悪くな
り、多すぎると子実体の形が正常でない場合かある。The amount of glucosamine added is preferably 0.05% by weight or more, more preferably 0.3 to 1.0% by weight, in the artificial medium. If there is too little glucosamine, the fruiting bodies will not grow properly, and if there is too much glucosamine, the shape of the fruiting bodies may not be normal.
グルコサミンの添加方法として、培地を調製する時に添
加してもよく、菌糸が成長した時点て子実体が発生する
直前に添加してもよい。さらに− 。Glucosamine may be added when preparing the medium, or may be added when the mycelium grows, just before the fruiting bodies develop. Furthermore-.
庶子実体を発生させた培地を子実体収穫後再使用する時
の添加も有効である。はだ木の場合も人工栽培に準じて
適用しグルコサミン又はその塩の水溶液中へほた木を浸
漬し、適宜含浸させることがてきる。It is also effective to add it when reusing the medium in which fruiting bodies have been generated after harvesting the fruiting bodies. In the case of bare wood, it can be applied in the same manner as artificial cultivation, by immersing the scallop into an aqueous solution of glucosamine or its salt to impregnate it as appropriate.
（発明の効果）
このようにしてつくられた培地てきのこ類を栽培すると
、比較的短時間で子実体の発生にまで至り、また発生す
る子実体の収量も従来のものに比しはるかに多く、肉質
のよい子実体が発生する。(Effects of the invention) When mushrooms are cultivated in the culture medium created in this way, fruiting bodies will develop in a relatively short period of time, and the yield of the fruiting bodies produced will be much higher than that of conventional methods. , fleshy fruiting bodies develop.
（実施例） 次に本発明を実施例に基づきさらに詳細に説明する。(Example) Next, the present invention will be explained in more detail based on examples.
実施例１
次の培地を調製した。ｐＨはＩＮ水酸化ナトリウム又は
ＩＮ塩酸で調整した。Example 1 The following culture medium was prepared. pH was adjusted with IN sodium hydroxide or IN hydrochloric acid.
ポテトデキストロース液体培地
ポテトエキス ２００．０ｇ／文グルコース
２０．０ｇ／立チアミン
１ ｍ ｇ ／立ｐＨ５，８
この培地５ｍｌを２０ｍ１容広口びんに入れ、これにＤ
−グルコサミン塩酸塩を０．５重量％を混合しく培地Ｂ
という）又は１．０重量％を混合して（培地Ｃという）
培地を調製し、これに供試用食用きのこ菌種としてエノ
キタケを接種、培養した。なおびんの口には布て軽く栓
をした。またコントロールとしてＤ−グルコサミン塩酸
塩を添加しない培地（培地Ａという）についてもエノキ
タケを接種、培養した。このようにしてエノキタケを２
０°Ｃの明所で２０日間培養し、菌糸の生育状況を観察
した結果、Ｄ−グルコサミン塩酸塩１．０重量％添加区
てはコントロールよりも菌糸の生育が少し劣るか０．５
重量％添加区では菌糸の生育がコントロールとほとんど
同してあった。Potato dextrose liquid medium potato extract 200.0g/gram glucose
20.0g/standing thiamin
1 mg/vertical pH 5.8 Pour 5 ml of this medium into a 20 ml wide-mouth bottle, and add D
-Medium B with 0.5% by weight of glucosamine hydrochloride
) or mixed with 1.0% by weight (called medium C)
A medium was prepared, and Enokitake mushroom was inoculated thereto as a test edible mushroom species and cultured. The mouth of the bottle was lightly stoppered with a cloth. As a control, enokitake mushrooms were also inoculated and cultured in a medium to which D-glucosamine hydrochloride was not added (referred to as medium A). In this way, make 2 enoki mushrooms.
After culturing in the light at 0°C for 20 days and observing the growth status of mycelia, it was found that the growth of mycelia was slightly inferior to the control in the group containing 1.0% by weight of D-glucosamine hydrochloride.
In the wt% addition plot, the growth of mycelium was almost the same as in the control.
菌糸の乾燥重量を第１表に示す。The dry weight of mycelium is shown in Table 1.
第 １ 表
上記と同様の培養試験を繰り返したところ培養後２０日
間で菌糸か蔓延したので菌かきし、さらに培養したとこ
ろ６６日目で添加区のサンプルびんすべてに子実体が見
られたが、コントロールには全く見られなかった。Table 1 When the same culture test as above was repeated, the mycelium spread after 20 days of culture, so the fungus was scraped off and further culture was carried out. On the 66th day, fruiting bodies were observed in all the sample bottles in the addition group. It was not seen at all in the control.
実施例２
２５０文容０水槽に水２００文を入れ、これにＤ−グル
コサミン塩酸塩１ｋｇを添加混合し、完全に溶解させた
。この水槽に予めシイタケの菌糸を蔓延させたほだ木（
直径的１０ｃｍ、長さ約１００ｃｍ）１０本を浸漬し、
水温１５°Ｃで２４時間静置した。その後、はた木を水
槽からひき上げ、シイタケ培養を行った。一方、Ｄ−ク
ルコサミン塩酸塩無添加の水を入れた水槽に同様のほた
木を浸漬した後、同様にシイタケ培養を行い、比較した
。子実体の収量及び大きさ別の個数を第２表に示す。Example 2 200 grams of water was placed in a 250-liter water tank, and 1 kg of D-glucosamine hydrochloride was added and mixed to completely dissolve it. This aquarium was pre-infested with shiitake mushroom mycelium (
Soak 10 pieces (10 cm in diameter and about 100 cm in length),
It was left standing at a water temperature of 15°C for 24 hours. Afterwards, we pulled the trees out of the aquarium and cultured the shiitake mushrooms. On the other hand, similar scallops were immersed in a water tank containing water without the addition of D-curcosamine hydrochloride, and then shiitake mushrooms were cultured in the same manner for comparison. Table 2 shows the yield and number of fruit bodies by size.
第２表
第２表の結果から明らかなように本発明によるグルコサ
ミン塩酸塩添加区は子実体収量か多く、また子実体も大
型、中型か６０％以上を占め肉質がよい。これに対し無
添加区では収量が少なく、小型の子実体が約６０％を占
める。As is clear from the results in Table 2, the glucosamine hydrochloride-added plot according to the present invention has a higher fruiting body yield, and the fruiting bodies are large or medium in size, accounting for 60% or more, and have good flesh quality. On the other hand, in the additive-free plot, the yield is low, with small fruit bodies accounting for about 60%.
Claims (1)
類を栽培することを特徴とするきのこ類の栽培方法。A method for cultivating mushrooms, which comprises cultivating mushrooms using a medium containing glucosamine or a salt thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63177396A JP2676375B2 (en) | 1987-08-05 | 1988-07-16 | How to grow mushrooms |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19515387 | 1987-08-05 | ||
| JP62-195153 | 1987-08-05 | ||
| JP63177396A JP2676375B2 (en) | 1987-08-05 | 1988-07-16 | How to grow mushrooms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01132315A true JPH01132315A (en) | 1989-05-24 |
| JP2676375B2 JP2676375B2 (en) | 1997-11-12 |
Family
ID=26497949
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63177396A Expired - Fee Related JP2676375B2 (en) | 1987-08-05 | 1988-07-16 | How to grow mushrooms |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2676375B2 (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100372814C (en) * | 2005-06-20 | 2008-03-05 | 付锡臣 | Organic fertilizer prepared by using waste blackfungus culture medium and its preparation method and use |
| US7514085B2 (en) | 2004-07-16 | 2009-04-07 | Medimush A/S | Immune modulating compounds from fungi |
| CN103960045A (en) * | 2014-05-14 | 2014-08-06 | 谢光玉 | Method for cultivating white funguses through fiber-shaped sugarcane stalks |
| US9072776B2 (en) | 2005-06-15 | 2015-07-07 | Glycanova As | Anti-cancer combination treatment and kit-of-parts |
| CN104871815A (en) * | 2015-05-11 | 2015-09-02 | 苏州葛家坞生物科技有限公司 | Method for preparing watermelon peels for planting snow fungus |
| US9249438B2 (en) | 2001-09-03 | 2016-02-02 | Glycanova As | Production of fungal extracellular immune stimulating compounds |
| CN107236674A (en) * | 2017-06-26 | 2017-10-10 | 浦江县美泽生物科技有限公司 | Edible fungus culture medium and preparation method thereof |
-
1988
- 1988-07-16 JP JP63177396A patent/JP2676375B2/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9249438B2 (en) | 2001-09-03 | 2016-02-02 | Glycanova As | Production of fungal extracellular immune stimulating compounds |
| US10471135B2 (en) | 2001-09-03 | 2019-11-12 | Glycanova As | Production of fungal extracellular immune stimulating compounds |
| US7514085B2 (en) | 2004-07-16 | 2009-04-07 | Medimush A/S | Immune modulating compounds from fungi |
| US7682615B2 (en) | 2004-07-16 | 2010-03-23 | Beka Holding As | Immune modulating compounds from fungi |
| US9072776B2 (en) | 2005-06-15 | 2015-07-07 | Glycanova As | Anti-cancer combination treatment and kit-of-parts |
| CN100372814C (en) * | 2005-06-20 | 2008-03-05 | 付锡臣 | Organic fertilizer prepared by using waste blackfungus culture medium and its preparation method and use |
| CN103960045A (en) * | 2014-05-14 | 2014-08-06 | 谢光玉 | Method for cultivating white funguses through fiber-shaped sugarcane stalks |
| CN104871815A (en) * | 2015-05-11 | 2015-09-02 | 苏州葛家坞生物科技有限公司 | Method for preparing watermelon peels for planting snow fungus |
| CN107236674A (en) * | 2017-06-26 | 2017-10-10 | 浦江县美泽生物科技有限公司 | Edible fungus culture medium and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2676375B2 (en) | 1997-11-12 |
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