JP2002125460A - Method for making mushroom bed of sparassis crispa rr. having physiologically functional activity - Google Patents
Method for making mushroom bed of sparassis crispa rr. having physiologically functional activityInfo
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- JP2002125460A JP2002125460A JP2000326881A JP2000326881A JP2002125460A JP 2002125460 A JP2002125460 A JP 2002125460A JP 2000326881 A JP2000326881 A JP 2000326881A JP 2000326881 A JP2000326881 A JP 2000326881A JP 2002125460 A JP2002125460 A JP 2002125460A
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Abstract
Description
【0001】[0001]
【発明の属する技術分野】本発明は、生理機能活性を有
するハナビラタケ(Sparassis Crispa Rr.)の菌床作製方
法に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for preparing a bacterial bed of Physcomitrella patens (Sparassis Crispa Rr.) Having a physiological function activity.
【0002】[0002]
【従来の技術】ハナビラタケは、カラマツに生える非常
に希少な茸であり、幻の茸とも称されている。該ハナビ
ラタケは歯ごたえが良いことに加えて、純白に近い淡黄
色の色合いおよび葉牡丹のような形態から、貴重な食材
として日本料理界でも珍重されている。2. Description of the Related Art Hanabitake mushrooms are extremely rare mushrooms that grow on Japanese larch, and are also called phantom mushrooms. In addition to its good chewyness, Hanabitake mushrooms are also prized in the Japanese cuisine world as valuable foodstuffs because of their pale yellow color close to pure white and forms like leaf peony.
【0003】更に、ハナビラタケには有用な生理活性物
質が多量に含まれており、この点でも注目されている。
特に、優れた免疫賦活作用を有する生理活性物質として
知られる総βグルカンの含有量は、100g当り43.
6mgである。総ベータグルカン含有量の多い茸として
知られるアガリクスやマイタケでも、その総ベータグル
カン含有量は、夫々100g当り11.6g、18.1
gであることと比較すれば、ハナビラタケに含まれる含
有量が驚異的に多いことが分かる。なお、総ベータグル
カンの中で、特にβ−1,3−D−グルカンについては
抗腫瘍効果が証明されている。従って、ハナビラタケは
健康食品として優れた食材であるのみならず、抗腫瘍剤
を製造するための貴重な原料である。Further, Hanabitake mushrooms contain a large amount of useful physiologically active substances, and attention has been paid to this point as well.
In particular, the content of total β-glucan known as a physiologically active substance having an excellent immunostimulating action is 43.
6 mg. Agaricus and maitake, also known as mushrooms with a high total beta-glucan content, have a total beta-glucan content of 11.6 g per 100 g and 18.1 respectively.
g, it can be seen that the content of Hanabitaketake is surprisingly high. In addition, among the total beta-glucan, in particular, β-1,3-D-glucan has been proven to have an antitumor effect. Therefore, Hanabiratake is not only an excellent food material as a health food, but also a valuable raw material for producing an antitumor agent.
【0004】上記のように優れた性質を有するにもかか
わらず、ハナビラタケは、幻の茸と称されることからも
明らかなように非常に希少であり、一般の食品として使
用するには高価すぎるという問題がある。人工栽培につ
いても種々試みられているが、カビ類に弱いという他の
茸にはない性質を有しているため人工栽培自体が困難で
あり、また運良く人工栽培に成功したとしても成長が遅
いため、未だ十分な成果を得るには至っていないのが現
状である。[0004] Despite having the above excellent properties, Hanabitake mushrooms are very rare, as evident from being called phantom mushrooms, and are too expensive to be used as general foods. There is a problem. Although various attempts have been made on artificial cultivation, artificial cultivation itself is difficult because it has a property that is not susceptible to fungi, which is not found in other mushrooms, and it is slow to grow even if it is lucky to succeed in artificial cultivation Therefore, at present, sufficient results have not yet been obtained.
【0005】更に、ハナビラタケの従来の人工栽培法に
おいては、その菌床作製の際に、培地に用いるカラマツ
大鋸屑を、予め熱水煮沸温度120〜121℃、圧力2
気圧の高温水蒸気に60分間さらした後、更に水洗する
ことによる培地処理が行われていた。これは、カラマツ
大鋸屑に含まれる阻害物質がハナビラタケの生育を阻害
するため、この阻害物質の除去を行わなければハナビラ
タケは順調に発育しないと言う理由に基づくものであ
る。しかし、この阻害物質の除去方法は操作が複雑であ
り、多額の費用がかかるため、菌床作製上の障害となっ
ていた。Further, in the conventional artificial cultivation method of Hanabiratake, larch sawdust used as a culture medium is previously heated to a boiling water temperature of 120 to 121 ° C. and a pressure of 2 at the time of preparing the bacterial bed.
After being exposed to high-pressure steam at atmospheric pressure for 60 minutes, the medium was treated by washing with water. This is based on the reason that the inhibitor contained in the larch sawdust inhibits the growth of Hanabitaketake, and unless this inhibitor is removed, Hanabitaketake does not grow smoothly. However, this method for removing the inhibitor is complicated in operation and requires a large amount of cost, which has been an obstacle in the preparation of a bacterial bed.
【0006】[0006]
【発明が解決しようとする課題】本発明は上記事情に鑑
みてなされたもので、その課題は、ハナビラタケを確実
に人工栽培することを可能にし、且つ従来の複雑かつ費
用のかかる培地処理を省略することが可能な、ハナビラ
タケの菌床作製方法を提供することである。DISCLOSURE OF THE INVENTION The present invention has been made in view of the above circumstances, and an object of the present invention is to make it possible to reliably cultivate Hanabitake mushrooms and to omit the conventional complicated and expensive medium treatment. It is an object of the present invention to provide a method for preparing a fungus bed of C. versicolor.
【0007】[0007]
【課題を解決するための手段】上記課題は、大鋸屑を主
成分とする培地に、ハナビラタケMH−3(受託番号F
ERM P−17221)を接種することを特徴とする
ハナビラタケの人工栽培用菌床の作製方法によって達成
される。Means for Solving the Problems The object of the present invention is to provide a medium containing sawdust as a main component, Hanabitaketake MH-3 (accession number F).
ERM P-17221), which is achieved by a method for producing a bacterial bed for artificial cultivation of Hanabitaketake.
【0008】本発明で使用するハナビラタケMH−3
は、人工栽培に適した菌株ものとして発明者が同定した
ものであり、平成11年2月17日に、受託番号FER
M P−17221の下に工業技術院生命工学工業技術
研究所に寄託された。この菌株を、大鋸屑を主成分とす
る培地に接種することにより作製した菌床を用いること
により、従来困難であったハナビラタケの人工栽培を可
能にすることができる。接種するハナビラタケMH−3
としては、その胞子または菌糸を用いるのが好ましい。[0008] Hanabitaketake MH-3 used in the present invention
Was identified by the inventor as a strain suitable for artificial cultivation, and on February 17, 1999, the accession number FER
Deposited with the Institute of Biotechnology and Industrial Technology under the MP-17221. By using a bacterial bed prepared by inoculating this strain into a medium mainly composed of sawdust, artificial cultivation of Hanabitake mushrooms, which has been difficult in the past, can be made possible. Inoculated Hanabitaketake MH-3
It is preferable to use the spores or hypha.
【0009】本発明により得られる菌床は、抗癌作用、
血糖値降下作用、血圧降下作用を有するβ−1,3−D
グルカンを多量に含有するハナビラタケ(茸)の工業的
人工栽培に使用するのに適している。The bacterial bed obtained by the present invention has an anticancer effect,
Β-1,3-D having blood glucose lowering action and blood pressure lowering action
It is suitable for industrial artificial cultivation of Hanabitake mushroom (mushroom) containing a large amount of glucan.
【0010】本発明においては、前記大鋸屑を主成分と
する培地に小麦粉を5〜10%添加して用いるのが好ま
しい。In the present invention, it is preferable to add 5 to 10% of flour to the above-mentioned medium containing sawdust as a main component.
【0011】本発明においては、前記カラマツ大鋸屑の
ハナビラタケ生育阻害物質を除去しないで用いることが
できる。In the present invention, the larch sawdust can be used without removing the growth inhibitory substance of the Japanese larch sawdust.
【0012】[0012]
【発明の実施の形態】ハナビラタケは、従来人工栽培が
行われている茸すなわち地中海シメジ、シイタケ、ヒラ
タケに比較して人工栽培が技術的に極めて困難であっ
た。しかし、本願発明者は鋭意研究の結果、菌の選別使
用(MH−3)により、目的とするβ−1,3−Dグル
カンを多量に含む茸の栽培を、従来の培地処理を必要と
することなく、短期間でかつ経済的に達することができ
た。また、栽培室の改善および培地組成の改良により、
更に改善された結果が得られた。BEST MODE FOR CARRYING OUT THE INVENTION The artificial cultivation of Hanabitake mushrooms has been extremely difficult technically in comparison with mushrooms conventionally subjected to artificial cultivation, namely Mediterranean shimeji, shiitake and oyster mushrooms. However, as a result of intensive research, the inventor of the present application has found that cultivation of mushrooms containing a large amount of the desired β-1,3-D glucan requires conventional medium treatment by selective use of bacteria (MH-3). Without any short and economical consequences. In addition, by improving the cultivation room and improving the medium composition,
Further improved results were obtained.
【0013】本発明において使用する培地は、ツガ、モ
ミ、マツ、ブナ、シイの大鋸屑を主成分とするものであ
る。これらの単一の大鋸屑もしくは混合物、またはその
チップに、ハナビラタケ(茸)の栽培に必要な栄養素を
混合して用いるのが好ましい。The medium used in the present invention is mainly composed of sawdust of hemlock, fir, pine, beech, and shii. It is preferable to use nutrients required for cultivating Hanabitake mushrooms in these single sawdust or mixture or chips thereof.
【0014】上記培地に接種するハナビラタケMH−3
としては、その胞子または菌糸を使用することができ
る。本発明においては、例えば、ツガ、モミ、マツ、ブ
ナ、シイの大鋸屑、またはチップにハナビラタケの発育
に必要な栄養素を混合し、これを500mLのポリエチ
レンビンに充填し、滅菌し、培地を常温まで冷却後、M
H−3の胞子または菌糸を無菌状態で接種する。こうし
て作製された菌床から子実体を栽培することにより、癌
の予防、治療、糖尿病、高血圧の治療に有効なβ−1,
3−Dグルカンを大量に含有するハナビラタケを栽培す
ることができる。[0014] Hanabitaketake MH-3 inoculated into the above medium
The spores or hyphae can be used. In the present invention, for example, sawdust, fir, pine, beech, shii sawdust, or chips are mixed with nutrients necessary for the growth of Hanabitake mushrooms, filled into a 500 mL polyethylene bottle, sterilized, and the medium is cooled to room temperature. After cooling, M
H-3 spores or hyphae are inoculated aseptically. By cultivating fruiting bodies from the fungal bed thus prepared, β-1, effective for cancer prevention, treatment, diabetes and hypertension treatment.
Hanabitake mushrooms containing a large amount of 3-D glucan can be grown.
【0015】本発明の第一の側面は、ハナビラタケMH
−3を使用することにより、高温水蒸気処理および水洗
といった従来の複雑かつ費用のかかる培地処理を必要と
せずに、ハナビラタケの人工栽培菌床の作製を可能にし
たことである。即ち、発明者はMH−3菌を使用するこ
とにより、上記の阻害物質除去操作を必要とせずに、ハ
ナビラタケを順調に生育せしめることに成功した。これ
により、極めて経済的かつ簡便にハナビラタケ菌床を作
製することが可能になった。また、従来ハナビラタケは
菌床の容器としてガラスビンを使用しなければ雑菌が混
入し、ハナビラタケの栽培は不可能とされていたが、M
H−3菌の使用によってポリエチレン容器を使用でき、
大型自動機械の使用が可能な工業化大量栽培が可能とな
った。[0015] The first aspect of the present invention relates to Hanabitaketake MH.
By using -3, it is possible to produce an artificially cultivated bacterial bed of Hanabitake mushroom without the need for conventional complicated and expensive medium treatment such as high-temperature steam treatment and water washing. That is, the inventor succeeded in successfully growing Hanabitaketake without using the above-mentioned inhibitory removal operation by using the MH-3 bacteria. As a result, it became possible to prepare the Hanabitake mushroom bed extremely economically and easily. In addition, conventionally, it was said that cultivation of Hanabiratake was impossible if various bacteria were mixed unless a glass bottle was used as a container for the fungus bed.
A polyethylene container can be used by using H-3 bacteria,
Industrialized mass cultivation that allows the use of large automatic machines has become possible.
【0016】本発明の第二の側面は、菌床培地の改良に
向けられている。上記のように、本発明ではハナビラタ
ケMH−3の菌床を作製するための培地として大鋸屑を
主成分に用いるが、これに添加する他の栄養素として小
麦を加えることにより、栽培期間の短縮および収穫量の
増大を達成することが可能になった。[0016] A second aspect of the present invention is directed to improvement of a bacterial bed medium. As described above, in the present invention, sawdust is used as a main component as a medium for preparing a fungus bed of Hanabitaketake MH-3, but by adding wheat as another nutrient to be added thereto, the cultivation period can be shortened and the harvest can be shortened. It has become possible to achieve increased amounts.
【0017】上記のようにして作製した菌床を用い、人
工栽培により選られたMH−3ハナビラタケは、抗癌作
用、血糖値降下作用、免疫賦活作用、および抗高血圧作
用が強力であることを発明者は発見した。この実験につ
いて以下に述べる。The MH-3 mushrooms selected by artificial cultivation using the bacterial bed prepared as described above show that the anti-cancer, hypoglycemic, immunostimulatory and anti-hypertensive effects are strong. The inventor has discovered. This experiment is described below.
【0018】 試験期間は35日間、使用したマウス
の体重約30g。このマウスにサルコーマ180型固形
肝ガンを移植した。この肝癌移植マウスに対して、試験
開始日より7,9,11日目の3回だけ、ハナビラタケ
(MH−3)湯水抽出のβ−1,3−Dグルカンを10
0ppm、500ppmを経口投与した。いずれの投与
量においても100%癌発生がなく、治癒した。The test period was 35 days and the weight of the mice used was about 30 g. Sarcoma 180 type solid liver cancer was transplanted to this mouse. To this liver cancer transplanted mouse, β-1,3-D glucan extracted from Hanabitake mushroom (MH-3) was extracted 10 times only on days 7, 9, and 11 from the test start date.
0 ppm and 500 ppm were orally administered. At all doses, 100% did not develop cancer and healed.
【0019】 自己免疫性糖尿病モデルであるNOD
マウス(約30g)に対して、ハナビラタケより抽出し
たβ−1,3−Dグルカン(100ppm、500pp
m)をそれぞれ経口投与したところ、いずれの投与量に
おいても発症が遅延または治癒した。NOD, an autoimmune diabetes model
For a mouse (about 30 g), β-1,3-D glucan extracted from Hanabitaketake (100 ppm, 500 pp)
When m) was orally administered, onset was delayed or cured at any dose.
【0020】 全身性自己免疫疾患糖尿病モデルのM
RL−1PL−1Prマウス(約30g)に対して、ハ
ナビラタケ抽出(MH−3)のβ−1,3−Dグルカン
(100ppm、500ppm)をそれぞれ経口投与す
ることにより、症状は改善された。M of the diabetes model for systemic autoimmune diseases
The symptom was improved by orally administering β-1,3-D glucan (100 ppm, 500 ppm) of Hanabitaketake (MH-3) to RL-1PL-1Pr mice (about 30 g).
【0021】 高血圧マウス(約30g)にハナビラ
タケ(MH−3)の温湯抽出β−1,3−Dグルカンを
50ppm、100ppm投与で、血圧は正常に戻り、
マウスの死亡を防止した。When hypertensive mice (approximately 30 g) were administered 50 ppm and 100 ppm of hot-water-extracted β-1,3-D glucan of Hanabitaketake (MH-3), the blood pressure returned to normal.
Mouse death was prevented.
【0022】[0022]
【実施例】実施例1 粉末バナナ6g、エビオス45g、ペプトン1g、カル
シウム0.6g、塩化マグネシウム0.5g、粉末蜂蜜
2g、小麦粉100gを混合した。次いで、これを1k
gのカラマツ大鋸屑(水分約20%)に添加し、常水
1.5Lを注加して混合した。EXAMPLE 1 6 g of powdered banana, 45 g of Ebios, 1 g of peptone, 0.6 g of calcium, 0.5 g of magnesium chloride, 2 g of powdered honey, and 100 g of flour were mixed. Then, this is 1k
g of larch sawdust (water content: about 20%), and 1.5 L of ordinary water was poured and mixed.
【0023】次に、上記で得た混合物をポリエチレン製
の500mL瓶に充填し、瓶の中心に直径1.5cmの
空気流通用の穴を開けて蓋をした後、100℃の蒸気滅
菌器に入れて5時間滅菌した。この方法によって、カラ
マツ大鋸屑のハナビラタケ発育防止物質による障害は防
止できるため、ハナビラタケ生育阻害物質の処理のため
に従来行われていた大鋸屑の加熱は必要としない。Next, the mixture obtained above was filled in a 500 mL bottle made of polyethylene, a hole for air circulation having a diameter of 1.5 cm was formed at the center of the bottle, and the bottle was covered with a steam sterilizer at 100 ° C. And sterilized for 5 hours. By this method, the larch sawdust can be prevented from being hindered by the growth inhibitor of Hanabitaketake, so that the heating of sawdust conventionally performed for treating the growth inhibitor of Hanabitaketake is not required.
【0024】滅菌した菌床を常温まで冷却したところ
で、MH−3菌(茸ハナビラタケ)の菌糸を無菌的に接
種した。接種後、20℃で1ヶ月のインキュベーション
の後に原基が発生した。容器の蓋を取り去り、温度20
℃、湿度80%の暗室に1ヶ月間放置したところ、目的
のβ−1,3−Dグルカンを大量に含有したハナビラタ
ケを収穫することができた。When the sterilized bed was cooled to room temperature, the mycelium of MH-3 (mushroom Hanabitaketake) was aseptically inoculated. After inoculation, primordia developed after incubation at 20 ° C. for one month. Remove the container lid and allow
When left in a dark room at 80 ° C. and a humidity of 80% for one month, it was possible to harvest Hanabitake mushrooms containing a large amount of the desired β-1,3-D glucan.
【0025】実施例2: 培地に小麦を添加したことに
よる効果 培地に小麦を添加しないで作製した菌床(培地A)と、
小麦を添加して作製した菌床(培地B)を作製した。こ
の両方の菌床を用いてハナビラタケの人工栽培を行い、
その結果を比較した。なお、培地Bは実施例1で使用し
た培地と同じものであり、菌床の作製方法は何れも実施
例1に記載したものと同様である。Example 2: Effect of the addition of wheat to the medium A bacterial bed (medium A) prepared without adding wheat to the medium,
A bacterial bed (medium B) was prepared by adding wheat. Artificial cultivation of Hanabiratake is performed using both of these bacterial beds,
The results were compared. The medium B is the same as the medium used in Example 1, and the method for preparing the bacterial bed is the same as that described in Example 1.
【0026】<培地(菌床)の組成> 培地A(小麦添加なし);粉末バナナ6g、ビール酵母
45g、ペプトン1g、塩化カルシウム0.6g、粉末
蜂蜜2g、カラマツ大鋸屑1kg 培地B(小麦添加あり);粉末バナナ6g、ビール酵母
45g、ペプトン1g、塩化カルシウム0.6g、粉末
蜂蜜2g、塩化マグネシウム0.5g、小麦粉100
g、カラマツ大鋸屑1kg <結果> 栽培経過 培地Aによる栽培と、培地Bによる栽培における経過を
以下の表1に示す。この経過から分かるとおり、菌接種
より原基の発生期間は、培地Aの場合の2ヶ月に対して
培地Bは1ヶ月であり、培地Bを用いることにより発生
期間を1/2に短縮することができた。<Composition of Medium (Bacterial Bed)> Medium A (without wheat); powdered banana 6 g, brewer's yeast 45 g, peptone 1 g, calcium chloride 0.6 g, powdered honey 2 g, larch sawdust 1 kg Medium B (with wheat added) ); Powdered banana 6g, brewer's yeast 45g, peptone 1g, calcium chloride 0.6g, powdered honey 2g, magnesium chloride 0.5g, flour 100
g, larch sawdust 1 kg <Results> Cultivation progress Table 1 below shows the progress in cultivation using medium A and cultivation using medium B. As can be seen from this process, the development period of the primordium from the bacterial inoculation is one month in the medium B for two months in the case of the medium A, and the development period is reduced to half by using the medium B. Was completed.
【0027】一方、子実体の発生からの収穫期間は、培
地Aおよび培地Bの何れの場合も1ヶ月であったが、菌
接種から収穫までの期間は、培地Aでは3ヶ月であるの
に対して培地Bは2ヶ月であった。この結果、培地Bを
使用することにより栽培期間が短縮することができた。
このことは、栽培サイクルの短縮により経済性を高め、
利益効率を向上できることを意味している。On the other hand, the harvesting period from the emergence of fruiting bodies was 1 month in both the medium A and the medium B. However, the period from the inoculation to the harvest was 3 months in the medium A. In contrast, medium B was 2 months. As a result, the cultivation period could be shortened by using the medium B.
This increases economy by shortening the cultivation cycle,
This means that profit efficiency can be improved.
【0028】[0028]
【表1】 [Table 1]
【0029】収穫量 培地Aを用い栽培と、培地Bを用いた栽培との間で、収
穫時の生ハナビラタケの生産量ならびにβ−1,3−D
グルカン重量を比較した。その結果を表2に示す。Amount of Harvest Between the cultivation using the medium A and the cultivation using the medium B, the production amount of the raw mushroom at the time of harvest and the β-1,3-D
The glucan weights were compared. Table 2 shows the results.
【0030】収穫時の100株当り平均で、培地Aは生
ハナビラタケ50.5g/1株に対し、培地Bは10
0.5g/1株で培地Aに比較して培地Bは約2倍の収
穫量であった。On average, the medium A was harvested at a rate of 50.5 g / raw strain of A. mushroom per 100 strains at the time of harvesting, and the medium B was 10
The yield of the medium B was about twice as large as that of the medium A at 0.5 g / 1 strain.
【0031】[0031]
【表2】 [Table 2]
【0032】表1、表2の栽培条件: ・カラマツ大鋸屑 ・菌株(茸ハナビラタケ)MH−3 ・菌株接種後、原基発生までの温度…18〜25℃、湿
度…55〜75% ・子実体発生より収穫までの温度…18〜25℃、湿度
…75〜85% β−1,3−Dグルカンの生産量を培地A、Bで比較す
るとAは35.8g/1株に対し、Bは45.2g/1
株であり、培地Aに比較して培地Bは約1.3倍の生産
が可能になり、経済的効果は培地Bによって極めて向上
した。Cultivation conditions in Tables 1 and 2: Larch sawdust ・ Strain (Mushroom Hanabiratake) MH-3 ・ Temperature after inoculation of the strain until primordia emergence: 18-25 ° C., humidity: 55-75% ・ Fruit body Temperature from emergence to harvest: 18-25 ° C, Humidity: 75-85% Comparing the production amount of β-1,3-D glucan in media A and B, A is 35.8 g / strain and B is 45.2g / 1
As a strain, the medium B was able to produce about 1.3 times as much as the medium A compared to the medium A, and the economic effect was significantly improved by the medium B.
【0033】[0033]
【発明の効果】MH−3菌を使用し、培地に添加栄養剤
に小麦粉を使用することにより以下の効果を得た。The following effects were obtained by using MH-3 bacteria and using flour as a nutrient added to the medium.
【0034】(1)菌床作製に使用するカラマツ大鋸屑
は、従来ハナビラタケの発育阻害を防止する目的で、熱
水煮沸・高温水蒸気処理・水洗が従来行われていたが、
本発明の方法によれば従来のこれらの処理が必要なく、
菌床作製に一工程が省けることにより経済効果が極めて
向上した。(1) Larch sawdust used for the preparation of the fungus bed has been conventionally subjected to boiling with hot water, high-temperature steam treatment and washing with water for the purpose of preventing growth inhibition of Hanabitake mushroom.
According to the method of the present invention, these conventional processes are not required,
The economic effect was greatly improved by eliminating one step for the preparation of the bacterial bed.
【0035】(2)本発明のB処方すなわち従来の処方
に5〜10%小麦粉を栄養剤として添加することでハナ
ビラタケの発育が促進され、さらに目的とするβ−1,
3−Dグルカンの量が従来法(A処方)に比較して約
1.3倍の増量が得られ、経済効果が格段に向上した。(2) The addition of 5 to 10% flour as a nutrient to the B-formulation of the present invention, ie, the conventional formula, promotes the growth of C. versicolor and further improves the desired β-1,
The amount of 3-D glucan was increased about 1.3 times as compared with the conventional method (formulation A), and the economic effect was remarkably improved.
【0036】(3)従来ハナビラタケはガラスビンを菌
床の容器として使用しなければ雑菌が混入しハナビラタ
ケの栽培は不可能とされていたが、MH−3菌の使用に
よってポリエチレン容器を使用でき、大型自動機械の使
用が可能な工業的大量栽培が可能となった。(3) Conventionally, it has been considered that cultivation of Hanabitake mushrooms is impossible due to contamination of various bacteria unless glass bottles are used as a container for the fungus bed, but polyethylene containers can be used by using MH-3 bacteria. Industrial mass cultivation that can use automatic machines has become possible.
─────────────────────────────────────────────────────
────────────────────────────────────────────────── ───
【手続補正書】[Procedure amendment]
【提出日】平成13年7月11日(2001.7.1
1)[Submission date] July 11, 2001 (2001.7.1)
1)
【手続補正1】[Procedure amendment 1]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】0003[Correction target item name] 0003
【補正方法】変更[Correction method] Change
【補正内容】[Correction contents]
【0003】更に、ハナビラタケには有用な生理活性物
質が多量に含まれており、この点でも注目されている。
特に、優れた免疫賦活作用を有する生理活性物質として
知られる総βグルカンの含有量は、100g当り43.
6gである。総ベータグルカン含有量の多い茸として知
られるアガリクスやマイタケでも、その総ベータグルカ
ン含有量は、夫々100g当り11.6g、18.1g
であることと比較すれば、ハナビラタケに含まれる含有
量が驚異的に多いことが分かる。なお、総ベータグルカ
ンの中で、特にβ−1,3−D−グルカンについては抗
腫瘍効果が証明されている。従って、ハナビラタケは健
康食品として優れた食材であるのみならず、抗腫瘍剤を
製造するための貴重な原料である。Further, Hanabitake mushrooms contain a large amount of useful physiologically active substances, and attention has been paid to this point as well.
In particular, the content of total β-glucan known as a physiologically active substance having an excellent immunostimulating action is 43.
6 g . Agaricus and maitake, which are known as mushrooms having a high total beta-glucan content, have a total beta-glucan content of 11.6 g and 18.1 g per 100 g, respectively.
It can be seen from the comparison with that that the content of Hanabitaketake is surprisingly high. In addition, among the total beta-glucan, in particular, β-1,3-D-glucan has been proven to have an antitumor effect. Therefore, Hanabitake mushrooms are not only excellent foods as health foods, but also valuable raw materials for producing antitumor agents.
Claims (4)
タケMH−3(受託番号FERM P−17221)を
接種することを特徴とするハナビラタケ人工栽培用菌床
の作製方法。1. A method for producing a fungus bed for artificial cultivation of Hanabitake mushrooms, which comprises inoculating Hanabitaketake MH-3 (Accession No. FERM P-17221) into a medium mainly containing sawdust.
除去するための、高温水蒸気処理および水洗の工程を行
わないことを特徴とする、請求項1または2に記載のハ
ナビラタケ人工栽培用菌床の作製方法。2. The method for producing a fungus bed for artificial cultivation of Hanabitake mushrooms according to claim 1 or 2, wherein a high-temperature steam treatment and a washing step for removing the growth inhibitory substances of Hanabitaketake in the culture medium are not performed. Method.
いることを特徴とする、請求項1または2に記載のハナ
ビラタケ人工栽培用菌床の作製方法。3. The method according to claim 1, wherein a plastic container is used as the bacterial bed container.
ことを特徴とする、請求項1〜3の何れか1項に記載の
ハナビラタケ人工栽培用菌床の作製方法。4. The method according to claim 1, wherein the culture medium contains 5 to 10% of flour.
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WO2004075627A1 (en) * | 2003-02-28 | 2004-09-10 | Yonekura, Yasuko | Vital culture of sparassic crispa and method of prparing growth medium |
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KR20030096548A (en) * | 2002-06-14 | 2003-12-31 | 박배한 | The Artificial Culture for Sparassis crispa Wulf. ex Fr using Sawdust |
WO2004075627A1 (en) * | 2003-02-28 | 2004-09-10 | Yonekura, Yasuko | Vital culture of sparassic crispa and method of prparing growth medium |
KR100555339B1 (en) * | 2004-01-20 | 2006-03-10 | 에코앤바이오 주식회사 | Method for cultivating phellinus linteus and apparatus for the method |
WO2005077148A1 (en) * | 2004-02-13 | 2005-08-25 | Toyama, Noriko | Hyphal culture of sparassis crispa and method of preparing composite culture medium |
JP2006075154A (en) * | 2004-08-09 | 2006-03-23 | Unitika Ltd | Method for artificially cultivating sparassia crispa |
KR100816504B1 (en) | 2005-11-03 | 2008-03-26 | 대한민국 | Cultivation method for cauliflower mushroom by use of steam-treated coniferous sawdusts |
JP2008230991A (en) * | 2007-03-19 | 2008-10-02 | Katsuragi Sangyo:Kk | BIOLOGICALLY ACTIVE COMPOSITION ORIGINATED FROM SPARASSIS CRISPA Wulf:Fr. |
JP2008271957A (en) * | 2007-03-31 | 2008-11-13 | Masanori Kobayashi | METHOD FOR PRODUCING SPARASSIS CRISPA AND beta-GLUCAN |
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