WO2005077148A1

WO2005077148A1 - Hyphal culture of sparassis crispa and method of preparing composite culture medium

Info

Publication number: WO2005077148A1
Application number: PCT/JP2004/001552
Authority: WO
Inventors: Yasuhiro Sekiguchi
Original assignee: Toyama, Noriko; Yonekura, Yasuko; Kobayashi, Fumiko; Mimura, Asuka
Priority date: 2004-02-13
Filing date: 2004-02-13
Publication date: 2005-08-25

Abstract

It has been a practice to employ a method comprising complicated procedures with the use of larch sawdust or a method wherein a specific material and a specific strain as well as an expensive nutrient are employed. To perform such a culture method on a large scale, great capital investment with the use of a specific material and a specific strain is required. Accordingly, Sparassis crispa cannot be produced thereby on a large scale. A tissue is separated from Sparassis crispa growing in nature and hyphae are cultured in an agar medium. Next, a seed culture is prepared by using conifer/deciduous mixed sawdust or conifer sawdust. Next, the seed culture is transplanted into a composite growth medium prepared by adding nutrients to the sawdust medium, thereby growing Sparassis crispa

Description

TECHNICAL FIELD The present invention relates to a mycelium culture and composite growth medium using Hanabitake mushrooms that have naturally grown in nature since ancient times. Background Art Conventional cultivation techniques include larch sawdust as pretreatment for the preparation of a fungal bed, boiling of sawdust as a work to remove hot water-soluble components of sawdust, and further immersion in high-pressure, high-temperature steam. This method uses germs, fir, pine, etc. and uses a specific fungus to add more expensive nutrients, so new capital expenditures and large production costs are required. Was an obstacle. DISCLOSURE OF THE INVENTION The present invention aims to improve the disadvantages of the conventional cultivation method and to develop a large-sized, high-quality, and inexpensive cultivation method for Hanabitake mushrooms in a short period of time. Cultivation in a medium containing agar as a main component, and transplantation into a mycelial growth medium containing a mixture of conifer and deciduous sawdust as a main component. Is inoculated in a polyethylene bag and weighing 2.5 kg. BEST MODE FOR CARRYING OUT THE INVENTION The mycelium of the present invention is composed of 2.5% agar as a main component and 0.4% of yeast extract in order to separate and multiply the tissue from Hanabitake mushroom, which has grown naturally since ancient times. Add 1.0 ml of purified water to 1.0% of Moltsect Structural 1.0% Sauce Loin, boil at 98 ° C for 25 minutes, and then place in a Petri dish with an inner diameter of 90 mm. Transfer m1 and aseptically cool to solution temperature of 12 ° C. Aseptic mushrooms are transplanted to this medium and cultured at room temperature 22 ° C and 75% humidity for about 10 days. The inoculum is prepared by mixing 20% of rice bran, 20% of bran, and 5% of corn cob as nutrients in a medium made mainly of mixed sawdust of softwood and deciduous trees to adjust the water content to 70%. Fill this into a culture bottle (850 ml), and make a hole with a diameter of 1.5 mm at the center. This is covered with a lid filled with air, sterilized at 98 ° C for 2.5 hours, and then cooled in an aseptic room to a temperature at which inoculation can be performed at 12 ° C. This medium is inoculated with the tissue-cultured bacteria, and cultured at room temperature 22 ° C and humidity 75%.

According to this method, the inoculum is produced without performing the treatment of the hot water-soluble component in the conventional method and without using the specific medium and the specific bacteria.

Cultivation is based on mixed sawdust of coniferous and deciduous trees, with the same nutrients as the above-mentioned inoculum, plus 0.3% of force-drain, and the polyethylene bag is filled with 2.5 kg and 2 inoculation holes are opened. , Close your mouth. The composite is sterilized at 120 ° C for 4.5 hours and cooled to the inoculatable temperature of 12 ° C. Then, disintegrate the seed culture cultivated in the bottle, and sow about 20 ml in the inoculation hole. This was placed at room temperature of 22 ° C to 23 ° C and humidity of 80%. By cutting the bag only in the area where the mushrooms of the primordia grow, and leaving it in place for about 15 days, you can harvest high-quality large-sized mushrooms. Industrial applicability Conventionally, cultivation of Hanabitake mushrooms has many problems in terms of economy and difficulty in preparing a culture medium, and has been a cultivation method under the constraints of culture media using specific bacteria and using specific main raw materials. . The present invention makes it possible to use native Nachinabitake fungi, to use a mixture of coniferous and deciduous trees for the cultivation medium and cultivation medium, and to use low-priced nutrients, thereby enabling the cultivation of high-quality Hanabitaketake. In addition, the cultivation facilities for raw food mushrooms, which are currently spread in the food market, can be diverted as they are, and the materials are the same, so that mass production of Hanabitake mushrooms is possible and economic revitalization of mushroom production farmers will be carried out. Seem.

Claims

The scope of the claims

1. A method in which the tissue of Hanabitake mushroom, which grows naturally since ancient times, is isolated and the mycelium is cultured in an agar-based medium.

2. A method for preparing a growth medium for cultivating Hanabitake mushrooms by inoculating the bacteria cultured by the above method into a composite medium mainly containing conifer, deciduous tree sawdust, or single conifer sawdust.