WO2004075627A1

WO2004075627A1 - Vital culture of sparassic crispa and method of prparing growth medium

Info

Publication number: WO2004075627A1
Application number: PCT/JP2003/002294
Authority: WO
Inventors: Yasuhiro Sekiguchi
Original assignee: Yonekura, Yasuko; Makiyama, Michiyo; Kobayashi, Fumiko; Mimura, Asuka
Priority date: 2003-02-28
Filing date: 2003-02-28
Publication date: 2004-09-10
Also published as: AU2003211432A1

Abstract

In the conventional art, it has been a practice to employ complicated procedures using larch sawdust or to use a specific material, a specific strain and, moreover, expensive nutrients. For mass-production by using these methods, an excessively large-scaled equipment is required. Because of using a specific material and a specific strain, it is impossible to produce Sparassic crispa on a mass scale. A tissue is separated from Sparassic crispa occurring in nature and cultured on an agar medium. Then, a seed culture is prepared in a conifer-deciduous sawdust mixture or conifer sawdust. Nutrients are further added to the above-described sawdust medium and the seed culture is transplanted therein. Thus, Sparassic crispa can be grown.

Description

Technical description Culture method of fungus and preparation of growth medium

TECHNICAL FIELD The present invention relates to a medium for culturing a bacterium and preparing a bacterium using a natural mushroom native to nature. Background art

Conventional cultivation techniques include larch sawdust as pretreatment for the preparation of bacterial beds, removal of hot water-soluble components from sawdust, boiling of sawdust and further immersion in high-pressure high-temperature steam, This was a method of using specific bacteria and adding more expensive nutrients to raw materials such as raw materials such as clogs, fir, and pine, which required new expensive equipment investment and large production costs. This was an obstacle for the preparation of bacterial beds. Disclosure of the invention

An object of the present invention is to improve the shortcomings of conventional cultivation methods and to develop a method for cultivating Hanabiratake mushrooms in a short period of time and at a low cost. And then inoculating the mycelium cultured by transplanting the mixture into a growth medium mainly containing mixed sawdust of coniferous and deciduous trees, into a cultivation medium in which the sawdust contains a growth nutrient. . BEST MODE FOR CARRYING OUT THE INVENTION

The mycelium of the present invention is composed of 2.5% agar as a main component and yeast extract 0.4% as a main component to isolate and cultivate the tissue from Hanabitake mushroom, which has grown naturally since ancient times. ■ Malt extract ι · ο% · Saccharose Add 100 ml of purified water to 10%, boil at 98 ° C for 25 minutes, transfer 25 ml to a Petri dish with an inner diameter of 90 mm, and aseptically cool to a solution temperature of 12 ° C. Aseptic transplantation of Hanabitake mushroom tissue into this medium, room temperature Incubate at 22 ° C · 75 ° / o humidity for about 10 days.

The inoculum is prepared as follows ^: A mixture of conifers and deciduous trees is used as a main ingredient, and a mixture of 20% rice bran, 20% bran, and 5% corn cob as nutrients is added to adjust the water content to 70%. Fill this into a culture bottle (capacity: 850 ml) and make a 1.5 mm diameter hole in the center. Add an air hole to this, sterilize it at 98 ° C for 2.5 hours, and then cool in an aseptic room to a temperature at which inoculation is possible at 12 ° C. This medium is inoculated with the bacteria isolated and cultured, and cultured at room temperature 22 ° C and humidity 75%.

According to this method, bacteria are prepared without performing the treatment of hot water-soluble components in the conventional method, and without using a specific medium and a specific bacterium.

For cultivation, further add 0.3% curdlan to the above inoculum medium, sterilize at 98 ° C for 2.5 hours, and cool to the inoculatable temperature of 12 ° C. This mechanically inoculated with inoculum primordium occurs by being enshrined approximately 15 days at 80% at room temperature 22 ^e C Humidity. By removing the shyness of the container and placing it in the same room for about 20 days, you can harvest Hanabitake mushrooms. Industrial applicability

Conventionally, cultivation of Hanabiratake has been a cultivation method with many problems in terms of preparation efficiency and difficulty in preparing a culture medium, and using a specific bacterium and restricting the culture medium using a specific main raw material. The present invention makes it possible to use native Hanabitake mushrooms, and to use a mixture of coniferous and deciduous trees for the cultivation medium and cultivation medium, and to use low-priced nutrients, enabling the cultivation of high-quality Hanabitaketake. In addition, the cultivation facilities for raw food mushrooms, which are currently popular in the food market, can be diverted as they are, and the materials and the like are the same, enabling mass production of Hanabitake mushrooms and revitalizing the economy of mushroom producers. Seem.

Claims

The scope of the claims

A method of isolating the indigenous Hanabitake mushroom tissue from ancient times and culturing the bacteria in a medium containing agar as a main component.

A method for preparing a fungal bed for cultivating Hanabitake mushrooms by inoculating the bacteria cultured by the above method into a mixed sawdust of softwood and deciduous trees or a medium mainly containing single sawdust of softwood.