NO753578L - - Google Patents
Info
- Publication number
- NO753578L NO753578L NO753578A NO753578A NO753578L NO 753578 L NO753578 L NO 753578L NO 753578 A NO753578 A NO 753578A NO 753578 A NO753578 A NO 753578A NO 753578 L NO753578 L NO 753578L
- Authority
- NO
- Norway
- Prior art keywords
- stated
- coli
- enterotoxin
- buffer
- carried out
- Prior art date
Links
- 239000000147 enterotoxin Substances 0.000 claims description 86
- 231100000655 enterotoxin Toxicity 0.000 claims description 86
- 101710146739 Enterotoxin Proteins 0.000 claims description 69
- 241000588724 Escherichia coli Species 0.000 claims description 69
- 238000000034 method Methods 0.000 claims description 67
- 239000000872 buffer Substances 0.000 claims description 47
- 238000000926 separation method Methods 0.000 claims description 28
- 102000030621 adenylate cyclase Human genes 0.000 claims description 25
- 108060000200 adenylate cyclase Proteins 0.000 claims description 25
- 239000000706 filtrate Substances 0.000 claims description 25
- 230000000694 effects Effects 0.000 claims description 19
- 239000000523 sample Substances 0.000 claims description 18
- 229920000936 Agarose Polymers 0.000 claims description 16
- 239000003792 electrolyte Substances 0.000 claims description 16
- 238000002523 gelfiltration Methods 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 16
- 238000002218 isotachophoresis Methods 0.000 claims description 13
- 239000002609 medium Substances 0.000 claims description 13
- 150000002270 gangliosides Chemical class 0.000 claims description 12
- 238000011534 incubation Methods 0.000 claims description 12
- 239000000047 product Substances 0.000 claims description 12
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 230000007895 enterotoxin activity Effects 0.000 claims description 11
- 239000000959 ampholyte mixture Substances 0.000 claims description 10
- 230000000369 enteropathogenic effect Effects 0.000 claims description 10
- 108010050327 trypticase-soy broth Proteins 0.000 claims description 10
- 239000003446 ligand Substances 0.000 claims description 9
- 239000001974 tryptic soy broth Substances 0.000 claims description 9
- 229920005654 Sephadex Polymers 0.000 claims description 8
- 239000012507 Sephadex™ Substances 0.000 claims description 8
- 238000010828 elution Methods 0.000 claims description 8
- 229920002401 polyacrylamide Polymers 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 7
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical group Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 6
- PVKSNHVPLWYQGJ-KQYNXXCUSA-N AMP-PNP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)NP(O)(O)=O)[C@@H](O)[C@H]1O PVKSNHVPLWYQGJ-KQYNXXCUSA-N 0.000 claims description 6
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 6
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 claims description 6
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- ZFXYFBGIUFBOJW-UHFFFAOYSA-N theophylline Chemical compound O=C1N(C)C(=O)N(C)C2=C1NC=N2 ZFXYFBGIUFBOJW-UHFFFAOYSA-N 0.000 claims description 6
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims description 5
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 5
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- 125000004122 cyclic group Chemical group 0.000 claims description 5
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- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 claims description 5
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- 235000012538 ammonium bicarbonate Nutrition 0.000 claims description 4
- 239000013068 control sample Substances 0.000 claims description 4
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 4
- 239000000194 fatty acid Substances 0.000 claims description 4
- 229930195729 fatty acid Natural products 0.000 claims description 4
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- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 3
- 150000003904 phospholipids Chemical class 0.000 claims description 3
- 125000006850 spacer group Chemical group 0.000 claims description 3
- 229960000278 theophylline Drugs 0.000 claims description 3
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical group [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 2
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- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 claims description 2
- YBVNFKZSMZGRAD-UHFFFAOYSA-N pentamidine isethionate Chemical compound OCCS(O)(=O)=O.OCCS(O)(=O)=O.C1=CC(C(=N)N)=CC=C1OCCCCCOC1=CC=C(C(N)=N)C=C1 YBVNFKZSMZGRAD-UHFFFAOYSA-N 0.000 claims description 2
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 claims 1
- 125000005122 aminoalkylamino group Chemical group 0.000 claims 1
- MWEQTWJABOLLOS-UHFFFAOYSA-L disodium;[[[5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-oxidophosphoryl] hydrogen phosphate;trihydrate Chemical compound O.O.O.[Na+].[Na+].C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)C(O)C1O MWEQTWJABOLLOS-UHFFFAOYSA-L 0.000 claims 1
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
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- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 10
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- 231100000757 Microbial toxin Toxicity 0.000 description 1
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- 229940063655 aluminum stearate Drugs 0.000 description 1
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- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
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- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 244000309466 calf Species 0.000 description 1
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- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
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- OWEZJUPKTBEISC-UHFFFAOYSA-N decane-1,1-diamine Chemical compound CCCCCCCCCC(N)N OWEZJUPKTBEISC-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
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- SFNALCNOMXIBKG-UHFFFAOYSA-N ethylene glycol monododecyl ether Chemical compound CCCCCCCCCCCCOCCO SFNALCNOMXIBKG-UHFFFAOYSA-N 0.000 description 1
- 239000002095 exotoxin Substances 0.000 description 1
- 231100000776 exotoxin Toxicity 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000001641 gel filtration chromatography Methods 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- WHWDWIHXSPCOKZ-UHFFFAOYSA-N hexahydrofarnesyl acetone Natural products CC(C)CCCC(C)CCCC(C)CCCC(C)=O WHWDWIHXSPCOKZ-UHFFFAOYSA-N 0.000 description 1
- SYECJBOWSGTPLU-UHFFFAOYSA-N hexane-1,1-diamine Chemical compound CCCCCC(N)N SYECJBOWSGTPLU-UHFFFAOYSA-N 0.000 description 1
- 244000052637 human pathogen Species 0.000 description 1
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- 150000002500 ions Chemical class 0.000 description 1
- RVPVRDXYQKGNMQ-UHFFFAOYSA-N lead(2+) Chemical compound [Pb+2] RVPVRDXYQKGNMQ-UHFFFAOYSA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
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- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
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- 201000003152 motion sickness Diseases 0.000 description 1
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- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
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- 239000004014 plasticizer Substances 0.000 description 1
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
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- 108090000765 processed proteins & peptides Proteins 0.000 description 1
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- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
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- 239000011780 sodium chloride Substances 0.000 description 1
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- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/025—Enterobacteriales, e.g. Enterobacter
- A61K39/0258—Escherichia
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH1467174A CH612994A5 (en) | 1974-11-01 | 1974-11-01 | Process for the preparation of coli enterotoxin |
| CH1645874 | 1974-12-11 | ||
| CH1645974 | 1974-12-11 | ||
| CH604075A CH615196A5 (en) | 1975-05-12 | 1975-05-12 | Purification process for coli enterotoxin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO753578L true NO753578L (enrdf_load_stackoverflow) | 1976-05-04 |
Family
ID=27428963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO753578A NO753578L (enrdf_load_stackoverflow) | 1974-11-01 | 1975-10-24 |
Country Status (16)
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4740585A (en) * | 1984-07-30 | 1988-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Synthetic vaccine against urinary infections |
| IL78775A (en) * | 1985-05-15 | 1992-06-21 | Biotech Australia Pty Ltd | Oral vaccines |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA738364B (en) * | 1972-12-04 | 1974-09-25 | Rit Rech Ind Therapeut | Piglet colibacillosis vaccines and process for their preparation |
-
1975
- 1975-10-22 DE DE19752547224 patent/DE2547224A1/de not_active Ceased
- 1975-10-23 DK DK478575A patent/DK478575A/da unknown
- 1975-10-23 FI FI752964A patent/FI752964A7/fi not_active Application Discontinuation
- 1975-10-24 NO NO753578A patent/NO753578L/no unknown
- 1975-10-28 GB GB44308/75A patent/GB1529871A/en not_active Expired
- 1975-10-28 NL NL7512590A patent/NL7512590A/xx not_active Application Discontinuation
- 1975-10-29 IE IE2352/75A patent/IE43702B1/en unknown
- 1975-10-30 CA CA238,673A patent/CA1053152A/en not_active Expired
- 1975-10-30 ES ES442242A patent/ES442242A1/es not_active Expired
- 1975-10-30 DD DD189144A patent/DD124879A5/xx unknown
- 1975-10-30 NZ NZ179108A patent/NZ179108A/xx unknown
- 1975-10-30 IL IL48384A patent/IL48384A/xx unknown
- 1975-10-31 FR FR7533471A patent/FR2289206A1/fr active Granted
- 1975-10-31 JP JP50132085A patent/JPS5170810A/ja active Pending
- 1975-11-03 AU AU86282/75A patent/AU511934B2/en not_active Expired
-
1978
- 1978-06-20 DK DK278478A patent/DK278478A/da unknown
- 1978-09-14 FI FI782827A patent/FI782827A7/fi not_active Application Discontinuation
-
1981
- 1981-01-23 SE SE8100404A patent/SE8100404L/sv unknown
Also Published As
| Publication number | Publication date |
|---|---|
| FI752964A7 (enrdf_load_stackoverflow) | 1976-05-02 |
| IE43702B1 (en) | 1981-05-06 |
| DK278478A (da) | 1978-06-20 |
| IE43702L (en) | 1976-05-01 |
| FR2289206B1 (enrdf_load_stackoverflow) | 1978-11-10 |
| SE8100404L (sv) | 1981-01-23 |
| IL48384A0 (en) | 1976-03-31 |
| FR2289206A1 (fr) | 1976-05-28 |
| DD124879A5 (enrdf_load_stackoverflow) | 1977-03-16 |
| CA1053152A (en) | 1979-04-24 |
| AU8628275A (en) | 1977-05-12 |
| IL48384A (en) | 1979-05-31 |
| DE2547224A1 (de) | 1976-05-13 |
| GB1529871A (en) | 1978-10-25 |
| NZ179108A (en) | 1978-07-28 |
| FI782827A7 (fi) | 1978-09-14 |
| ES442242A1 (es) | 1977-08-01 |
| DK478575A (da) | 1976-05-02 |
| NL7512590A (en) | 1976-05-04 |
| JPS5170810A (enrdf_load_stackoverflow) | 1976-06-18 |
| AU511934B2 (en) | 1980-09-11 |
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