NO345471B1 - Pharmaceutical compositions for preventing or treating a sea lice disease comprising an extract of Sophora flavescens Aiton or fractions thereof. - Google Patents
Pharmaceutical compositions for preventing or treating a sea lice disease comprising an extract of Sophora flavescens Aiton or fractions thereof.Info
- Publication number
- NO345471B1 NO345471B1 NO20181070A NO20181070A NO345471B1 NO 345471 B1 NO345471 B1 NO 345471B1 NO 20181070 A NO20181070 A NO 20181070A NO 20181070 A NO20181070 A NO 20181070A NO 345471 B1 NO345471 B1 NO 345471B1
- Authority
- NO
- Norway
- Prior art keywords
- extract
- sophora flavescens
- sea lice
- flavescens aiton
- fish
- Prior art date
Links
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- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000001846 repelling effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/10—Anthelmintics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Fodder In General (AREA)
- Medicines Containing Plant Substances (AREA)
- Feed For Specific Animals (AREA)
Description
[Technical Field]
[1] The present invention relates to a pharmaceutical composition for preventing or treating a sea lice infectious disease, comprising an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredientand a feed additive composition for preventing or improving a sea lice infection.
[Background Art]
[2] Worldwide, fish aquaculture is an alternative to the depletion of fishery resources, focusing on fish species that are favorable to food and have excellent merchantability, and among them, salmon contains many unsaturated fatty acids such as DHA and EPA which are beneficial to our body, and is excellent in taste and is the most preferred fish species in the world. The world's largest salmon aquaculture country is Norway, followed by Britain, Chile, Canada and Australia. The common point of salmon cultivation stations is that they are located in the waters where cold seawater flows. The global salmon market is estimated at 4.3 million tons and 20 trillion won a year, including about 60 trillion won if it includes processing market.
[3] In Korea, Salmonidae, Cherry salmon is mainly cultivated, and Cherry salmon is a salmonidae living in rivers and seas of Korea, China, Japan and Russia, and the size is 60 cm smaller than 1 meter of Norwegian salmon. We call it Shima salmon which is Japanese name. That is, Salmonidae trout inhabitats in Korea, but they do not use the name salmon but cherry salmon (trout). Trout fishing and festivals in winter are famous. In Korea, the development of freshwater-based inland water trout farming has developed, and in 2015 it was the 50th anniversary of trout farming. Meanwhile, salmon can be classified into Pacific salmon and Atlantic salmon. In Korea, silver salmon belonging to Pacific salmon is being tried. Atlantic salmon, on the other hand, are cultivated in large scale in the North Sea fjord such as Norway, and in the Pacific coast in Chile and Canada. Korea is still in trial study.
[4] Norway's most known form of salmon, Marine Harvest, succeeded in salmon farming in the 1960s, and small businesses with local fishermen have grown into global companies today. Currently, the largest salmon farming company in the world, hatches 100 million salmon eggs per year and earns about 3 trillion won in salmon farming.
[5] However, the biggest problem of the current aquaculture industry is mortality rate, which is more than 20%, and the major causes are the incidence of intractable diseases and infections (viral, bacterial, parasitic) due to the rise in water temperature, and reduction of the effects of medicines treatment. In particular, "sea lice" is essential for farms with high breeding density, and salmon with sea lice has many side effects due to physical and chemical damage. 'Sea lice' is an external parasite that is largely parasitic to Atlantic salmon farmed in Norway as well as Scotland and Canada. Once a sea lice occurs, it can spread explosively. Specifically, sea lice for fish are external parasites that live on the mucus, epidermal tissue or blood of the host fish. The protein components present in the sea lice are attached to the in vitro tissues of the fish and become parasitic life as a host. When sea lice are parasitized on fish, they amplify inflammation, pathogens, etc., and they have a serious problem that the mortality rate increases and the fish population decreases. In Norway and Scotland, there are cases where salmon are killed by 30 to 40% because fish are concentrated and cultivated intensively within the cage farm.
[6] In the past, farmers tried to solve this problem by putting insecticides on feed for salmon farming, but in recent years, sea lice resistant to this chemical has appeared. Currently, Marine Harvest is trying to reduce the number of sea lice by introducing a feeder fish which naturally eats the sea lice into the fish farm. Experts predict that sea lice extermination will be possible with convergence technologies, including old management means such as pesticides and new strategies such as genetic resistance culture, but the performance is not yet successful, and to date, the use of insecticides containing emamectin benzoate has been the most effective means.
[7] [Prior art])
CN105029014A discloses a feed composition that improves immune function and improves resistance to bacteria, fungi, and parasites, including 0.2-0.3 parts by weight of Sophora Flavescens extract among various ingredients and the main ingredient contains 50-70 parts by weight of pine needle. No information on sea lice causing diseases are described.
CN101040936A discloses the use of 15-25% of radix Sophora Flavescens extract among various ingredients for fish parasites but no information on sea lice causing disease are described.
CN101690767A discloses the use of 1-50 parts by weight of radix Sophora Flavescentis extract among various ingredients for fish parasites, but no information on sea lice causing disease are described.
Jung, Sung Hee in “Studies on prophylactic effect of natural substances against
Fish disease". Project Report of National Fisheries Research and Development
Institute, 2010, describes the effect of Sophora Flavescens extract for fish parasites, but no information on sea lice causing disease are described.
DETAILED DESCRIPTION OF THE INVENTION
[Summary]
[8] The present invention provides a pharmaceutical composition for preventing or treating a sea lice infectious disease, using a Sophora flavescens Aiton, which is a food material harmless to human body and environmentally friendly and without causing tolerance, toxicity and environmental pollution.
[9] It is another object of the present invention provides a feed additive composition for preventing or improving a sea lice infection comprising the extract of Sophora flavescens Aiton or fraction thereof as an active ingredient.
[Technical Solution]
[10] The present invention provides a pharmaceutical composition for preventing or treating a sea lice infectious disease comprising an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredient. The extract of Sophora flavescens Aiton is a leaf, stem, root, flower, fruit or whole of Sophora flavescens Aiton, and extracts include matrine, oxymatrine, anagyrine, sophoranol, cytisine, methylcytisine, kurarinone, xanthohumol, Noranhydroicaritin, isoanhydroicaritin, glucoluteolin, kaempferol-3-sophoroside, Heilsteine, and Heilsteine-4-rhamnosyl glucoside.
[11] The extract of Sophora flavescens Aiton according to the present invention can be obtained by ultrasonic extraction, hot water extraction or solvent extraction, but is not limited thereto.
[12] In the present invention, the sea liceis of the genus Lepeophtheirus or the genus Caligus, , and more preferably, it is Lepeophtheirus salmonis or caligus rogercresseyi.
[13] The fish can be collectively referred to as any fish to which the parasitic infectious disease according to the present invention can develop, and it is preferable to use salmon, trout, catfish, perch, tuna, halibut, arctic char, sturgeon, flounder, tonguefish, carp, tilapia, striped bass, eel, sea bream, yellow tail, amber jack, grouper, Korean rockfish, red sea bream, rock bream, flat fish, mackerel, sea bass, tuna blue-fin, black sea bream, yellow tail and goldstriped amberjack. It is also preferably, but not exclusively, aquaculture. According to the present invetion, the fish is Salmonidae.
[14] The present invention also provides a feed additive composition for preventing or improving a sea lice infection comprising an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredient.
[15] It is also described a composition for the control or insects of a fish parasite comprising Sophora flavescens Aiton extract or a fraction thereof as an active ingredient.
[16] It is also described a method for preventing or treating parasitic infestation in a fish by injecting a composition comprising an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredient into fish.
[Effects of the Invention]
[17] The composition comprising the extract of Sophora flavescens Aiton of the present invention or the fraction thereof as an active ingredient may be used as a preventive or therapeutic agent for sea lice because it induces sea lice to be avoided or the fish to inhibit or eliminate parasitic access at the time of ingestion.
BRIEF DESCRIPTION OF THE DRAWINGS
[18] Fig.1 shows a Y-tube chamber for sea lice avoidance experiments.
[19] Fig. 2 shows the results of the sea lice avoidance effect of the extract of Sophora flavescens Aiton.
[20] Figures 3(a) and 3(b) show the death, blunting, and survival rate of sea lice in a concentration-dependent manner.
DETAILED DESCRIPTION
[21] Sophora flavescens Aiton is a perennial plant belonging to the rosemary bean family of dicotyledonous plants and a wild flower native to the mountain areas of the country and flowers bloom in raceme.
[22] The scientific name of Gosam is Sophorae flavescens Aiton, and the name Gosam uses the word 'Go' because the taste is bitter and use the letter 'Sam' because the efficacy is similar to ginseng. It’s roots are mainly used as medicines, and it has the effect of elimination of stroke, the cooling the heat of the body, the efficacy of taking urine out of it and extracting heat through it and the efficacy of cooling the heat and drying the moisture, and therefore, it is used for leukopenia treatment and antiradioactivity, blood sugar lowering, antitumor, antibacterial, immunosuppression and so on.
[23] In the present invention, Sophorae flavescens Aiton is used as raw, or it is possible to use a Sophorae flavescens Aiton which has undergone more than one process through a drying method such as natural drying, oven drying, hot air drying, freeze drying, or a method such as juicing, grinding, cutting and crushing.
[24] The term “extract” in the present invention means an extract obtained by extracting the Sophorae flavescens Aiton, a diluent or concentrate of the extract, a dried product obtained by drying the extract, an adjusted product or a purified product of the extract, or a mixture thereof, and extracts of all formulations which can be formed using an extract.
[25] In the extract of Sophora flavescens Aiton of the present invention, the method for extracting Sophora flavescens Aiton is not particularly limited and may be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction method, ultrasonic extraction method, filtration method, and reflux extraction method. These methods may be carried out singly or in combination of two or more methods.
[26] In the present invention, the kind of the extraction solvent used for extracting the Sophora flavescens Aiton is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; or mixtures thereof, and preferably, water, lower alcohol, 1,3-butyleneglycol, and ethyl acetate may be used alone or in combination of two or more.
In the present invention, the extract may be extracted with a solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms and a mixed solvent thereof, and the alcohol may be methanol, ethanol, propyl alcohol or butanol, but is not limited to.
[27] As a method for obtaining the extract of Sophora flavescens Aiton of the present invention, it is more preferable to dry the Sophora flavescens Aiton at a shade and a room temperature for 5 to 7 days after harvest, and to pulverize, and polar solvent of (C1) to (C4) lower alcohols such as water, methanol, ethanol, and butanol of about 1 to 20 times, preferably about 1 to 5 times, of the dry weight, or a mixed solvent thereof having a mixing ratio of about 1: 0.1 to 1:10 is used as an elution solvent, and the extraction temperature is 20 <o >C to 100 <o >C, preferably room temperature, and the extraction period can be extracted using extraction methods such as hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction for about 12 hours to 6 days, preferably 5 days.
[28] The term “fraction” in the present invention means the product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.
[29] The fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art. As a non-limiting example of the above-mentioned fractionation method, there can be mentioned a method in which the extract obtained by extracting Sophora flavescens Aiton is treated with a predetermined solvent to obtain a fraction from the extract.
[30] In the present invention, the compounds contained in the extract of Sophora flavescens Aiton include matrine, oxymatrine, anagyrine, sophoranol, cytisine, methylcytisine, kaurinone, xanthohumol, Noranhydroicaritin, isoanhydroicaritin, glucoluteolin, kaempferol-3-sophoroside, Heilsteine, Heilsteine-4-rhamnosyl glucoside, and the like. And such compounds may be obtained from the extract or fraction by conventional separation and purification processes.
[32]Fish can be collectively referred to as any fish to which the sea lice infectious disease according to the present invention can develop, and it is preferable to use salmon, trout, catfish, perch, tuna, halibut, arctic char, sturgeon, flounder, tonguefish, carp, tilapia, striped bass, eel, sea bream, yellow tail, amber jack, grouper, Korean rockfish, red sea bream, rock bream, flat fish, mackerel, sea bass, tuna blue-fin, black sea bream, yellow tail and goldstriped amberjack. It is also preferably, but not exclusively, aquaculture. In one embodiment of the present invention, an experiment was conducted using salmon as an example.
[33] A parasite collectively refers to an invertebrate living in parasitic life outside and in the body of another organism (fish, crustacean, animal, plant, etc.). In detail, there are the sea lice, Cymothoa Exigua, Ichthyophthirius multifiliis, Bivagina tai, Anisakiasis, Distoma, Tapeworm, Scutica, Benedenia, Anisakis Simplex, and the like. Also internal parasites (protozoa, annular animals, linear animals, etc.) and external parasites are included in the term (flat animals, arthropods, etc.).
[34] According to the present invention, it has been confirmed that the extract of Sophora flavescens Aiton has an effect of eliminating sea lice (FIGS. 2 and 3), and therefore, it has been found that the composition comprising the extract of Sophora flavescens Aiton of the present invention is suitable for use in the prevention, improvement or treatment of a sea lice infectious disease.
[35] The “prevention” in the present invention means any action to inhibit or delay the onset of a sea lice infectious disease of fish by the composition, and “treatment” means any action whereby the symptoms of a sea lice infectious disease of a fish are improved or beneficially altered by the composition.
[36] The “pharmaceutical composition” in the present invention means a preparation for the purpose of prevention or treatment of disease, and may be formulated into various forms according to conventional methods. For example, it can be formulated into oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions and syrups, and can be formulated in the form of external preparations, suppositories, and sterilized injection solutions. However, it is most preferable that the composition of the present invention is provided in the form of a bathing agent for the purpose of treating diseases of fishes.
[37] In addition, each of the formulations may be further formulated with pharmaceutically acceptable carriers such as buffers, preserving agents, wettability agents, solubilizers, isotonic agents, stabilizers, bases, excipients, lubricants and the like known in the art.
[38] Meanwhile, the pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. The “ pharmaceutically effective amount” of the present invention means an amount that is sufficient to treat a disease at a reasonable benefit / risk ratio applicable to veterinary treatment and does not cause side effects, and “effective dose levels” may be determined by factors including the health of a fish, severity, activity of the drug, sensitivity to the drug, method of administration, time of administration, route of administration and rate of release, duration of treatment, composition or factors including co-administered drugs and other factors well known in the veterinary arts.
[40] In another aspect, the present invention provides a feed additive composition for preventing or improving a sea lice infection, comprising an extract of Sophora flavescens Aiton or a fraction thereofas an active ingredient.
[42] For the purpose of the present invention, the extract of Sophora flavescens Aiton or fractions thereof, can be added to feed compositions as a feed additive, since they can be used for the purpose of preventing or improving a sea lice infectious disease of fish.
[43] In the present invention, the feed additives are collectively referred to as substances added to feed in small quantities for nutritive or specific purposes, therefore, in the present invention, the feed additives are substances added for the purpose of preventing or improving sea lice infectious diseases of fish. The extract of Sophora flavescens Aiton of the present invention or fraction thereof may be included in the feed composition as the feed additive, and the feed may be used to collectively mean all the foods supplied to the fish.
[44] The feed composition of the present invention may further contain a binder, an emulsifying agent, a preservative, etc. to be added for the purpose of preventing deterioration of quality, and may further contain an amino acid, a vitamin, an enzyme, a prophylactic agent, a flavoring agent, a non-protein nitrogenous substance, a silicate, a buffer, a colorant, an extractant, an oligosaccharide and the like for the purpose of enhancing the utility, and in addition, feed mixtures and the like may be further included, but the present invention is not limited thereto.
[45] The compound feed commonly used in the feed composition for fish includes at least one protein source selected from fish meal, casein, meat meal, and shrimp powder, or at least one carbohydrate source selected from sweet potato starch, potato starch, wheat starch, or at least one lipid source selected from rapeseed oil, soybean oil, corn oil, pollack liver oil, squid liver oil, but the present invention is not limited thereto, and even when used in combination with the compounded feed, the effect of preventing or improving the parasitic infectious disease described in the present invention occurs in the same manner.
[46] It is also described a method for preventing or treating parasitic infestation in a fish, comprising the step of administering a composition comprising the extract of Sophora flavescens Aiton, a fraction thereof or a compound isolated therefrom as an active ingredient directly to a fish or to a fish farm.
[47] It is also described a method for preventing or treating parasitic infestation in fish, comprising the step of administering to a fish directly or in a fish farm a pharmaceutical composition or feed additive composition comprising an extract of Sophora flavescens Aiton as an active ingredient.
[48] The method for preventing or treating a parasitic infectious disease of fish may include any step directly or indirectly delivered to a fish by including the step of administering directly to the fish or to a farm.
[49]The purpose of the present invention can be achieved by directly injecting or feeding to a fish, and can also be accomplished through means such as bathing or eating. Preferably, the effective concentration of the composition may be coated on the feed and fed to a fish having lesions due to parasitic infectious disease. At this time, the dose of the composition may vary depending on the weight of the fish to be treated, the health condition, the administration time, and the severity of the disease.
[51] Hereinafter, effects of the present invention will be described with reference to examples.
[53] [Example 1]: Preparation of extract of Sophora flavescens Aiton
[54] The roots were thoroughly washed with water, naturally dried for 5 to 7 days, and cut. Three times the weight of methanol was added to 50 kg of the excised Sophora flavescens Aiton, and the mixture was extracted at room temperature for 5 days and then filtered. It was concentrated under reduced pressure, lyophilized and powdered.
[56] [Example 2]: Sea lice (avoidance) repellent effect of Sophora flavescens Aiton [57] Using the Y-Tube choice camber, the movement of the sea lice and the time spent in each section were measured to confirm the avoidance effect of the sea lice repellent.
[58] The control section is flushed with clean sea water and the compound section is flushed with the substance to be tested. Mixed section is a section where control and compound sections are mixed and drained.
[59] The sea lice are positioned at the point where the mixed section starts and the movement is observed, and the analysis of the test results confirmed the effect of sea lice eradication by analyzing how long the sea lice moved and stayed in the opposite direction of flow of the test material, that is, the control section and the mixed section.(see Fig. 1)
[61] According to the above experimental method, the movement of the sea lice was observed by flowing the seawater in the control section and flowing the substance of the present invention in the compound section, as shown in FIG. 2.
[62] In Fig.2, Con.1 was treated with seawater as a control, OH4_A was treated with 0.5 mg / ml of Sophora flavescens Aiton and OH4_B was treated with 0.25 mg / ml of Sophora flavescens Aiton respectively and therefore it shows the effect of avoiding sea lice. In Con.1, the sea lice did not move to other sections because the seawater flows in all sections of the Y-tube chamber, and the time spent of sea lice in OH4_A treated with 0.5 mg / ml Sophora flavescens Aiton was about 68% in the control section, about 20% in the mixed section, and about 12% in the compound section, and the time spent of sea lice in OH4_B treated with 0.25 mg / ml of Sophora flavescens Aiton was about 29% in the control section, about 24% in the mixed section, and about 47% in the compound section. This means that it has sea lice avoidance effect depending on the concentration of Sophora flavescens Aiton.
[64] [Example 3]: The survival rate of sea lice about Sophora flavescens Aiton.
[65] In order to confirm the effectiveness of Sophora flavescens Aiton that the sea lice had a repelling effect, experiments were carried out using a 24-well plate. Sea water, sea lice and active substances were added to the 24-well plate at various concentrations, and then incubated in an incubator, and all solutions used in the experiments were kept at 10 ° C during the experiment. After incubation for 24 hours, the effect of sea lice was confirmed by observing the condition of sea lice.
[67] As a result of the experiment, as shown in Fig. 3 (a), about 95% of the sea lice survive in the control without any treatment, but as the treatment concentration of extract of Sophora flavescens Aiton increased, the number of alive species decreased and the number of dead species increased. In addition, when the concentration of extract of Sophora flavescens Aiton was 0.31 mg / ml, 50% (LD50) of the sea lice population was dead, and at 0.12 mg / ml, the sea lice population 50% (EC50) showed moribund state or swimming inhibition and showed excellent effect on sea lice.
[69] In conclusion, the extract of Sophora flavescens Aiton of the present invention or its fractions thereof have anti-sea lice activity, and thus can be used for the prevention or treatment of sea lice infectious diseases of fish, for the sea lice elimination, and for the control thereof.
[INDUSTRIAL APPLICABILITY]
The present invention is recognized as being industrially applicable in the fields of pharmaceutical biotechnology and agriculture and fisheries.
Claims (8)
1. A pharmaceutical composition for preventing or treating a sea lice infectious disease comprising an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredient.
2. The pharmaceutical composition according to claim 1, wherein the extract of Sophora flavescens Aiton is leaf, stem, root, fruit or whole of Sophora flavescens Aiton.
3. The pharmaceutical composition according to claim 1, wherein the the extract of Sophora flavescens Aiton comprises at least one compound selected from the group consisting of matrine, oxymatrine, anagyrine, sophoranol, cytisine, methylcytisine, kaurinone, xanthohumol, Noranhydroicaritin, isoanhydroicaritin, glucoluteolin, kaempferol-3-sophoroside, Heilsteine, and Heilsteine-4-rhamnosyl glucoside.
4. The pharmaceutical composition according to claim 1, wherein the extract of Sophora flavescens Aiton is subjected to ultrasonic extraction, hot water extraction or solvent extraction.
5. The pharmaceutical composition according to claim 1, wherein the sea lice is of the genus Lepeophtheirus or of the genus Caligus.
6. The pharmaceutical composition according to claim 5, wherein the sea lice is Lepeoptheirus salmonis or caligus rogercresseyi.
7. The pharmaceutical composition according to claim 1, wherein the fish is Salmonidae.
8. A feed additive composition for preventing or improving a sea lice infection comprising the extract of Sophora flavescens Aiton or fraction thereof as an active ingredient.
Oversettelse til norsk av godkjent kravsett
Norsk søknad 20181070
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/KR2018/001249 WO2019146826A1 (en) | 2018-01-29 | 2018-01-29 | Composition for preventing or treating parasitic infections in fish, comprising sophora flavescens aiton extract or fraction thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO20181070A1 NO20181070A1 (en) | 2018-08-14 |
| NO345471B1 true NO345471B1 (en) | 2021-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| NO20181070A NO345471B1 (en) | 2018-01-29 | 2018-01-29 | Pharmaceutical compositions for preventing or treating a sea lice disease comprising an extract of Sophora flavescens Aiton or fractions thereof. |
Country Status (4)
| Country | Link |
|---|---|
| CA (1) | CA3088618C (en) |
| GB (1) | GB2583664B (en) |
| NO (1) | NO345471B1 (en) |
| WO (1) | WO2019146826A1 (en) |
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| CN113499337B (en) * | 2021-08-11 | 2023-04-28 | 江苏海洋大学 | Application of kurarinone in preparation of aquatic pathogenic bacteria resistant medicines or feed additives |
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| CN101040936A (en) * | 2007-04-28 | 2007-09-26 | 大连水产学院 | Compound Chinese traditional medicine agent for preventing fish verminosis |
| CN101690767A (en) * | 2009-09-29 | 2010-04-07 | 北京大北农动物保健科技有限责任公司 | Compound pesticide for fishing and preparation method and application thereof |
| CN105029014A (en) * | 2015-07-08 | 2015-11-11 | 滁州市南谯区瑞琳水产养殖专业合作社 | Pine needle soft feed for reducing fish dermatopathy and preparation method thereof |
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| JP4608255B2 (en) * | 2004-07-15 | 2011-01-12 | 日本水産株式会社 | Composition having anti-parasitic activity for fish and method for preventing / treating parasitic disease |
| KR101006556B1 (en) * | 2007-12-20 | 2011-01-07 | 주식회사 바이넥스 | Anti-bacterial compositions against Streptococcus |
| KR101207276B1 (en) * | 2010-04-14 | 2012-12-13 | 대한민국 | Feed additives using Sophora flavescens Ait and Feed for raising fishes including the same |
| JP5954252B2 (en) * | 2012-05-16 | 2016-07-20 | 信越化学工業株式会社 | Resist material and pattern forming method using the same |
| CN103877459A (en) * | 2012-12-20 | 2014-06-25 | 青岛中仁药业有限公司 | Compound Chinese herbal medicine capable of treating grass carp ichthyophthirius multifiliis and its production method |
| KR101813078B1 (en) * | 2015-10-27 | 2017-12-29 | (주)나리소 | Insecticide Composition Containing Plant Extracts as Active Ingredients |
| CN105232850A (en) * | 2015-11-11 | 2016-01-13 | 郑州后羿制药有限公司 | Chinese herbal medicine fermentative extracting solution for preventing and controlling parasitic diseases of freshwater fish and preparation method thereof |
| CN106265628B (en) * | 2016-07-18 | 2018-12-14 | 暨南大学 | Sophoraflavanone G kills the application in fish epizoite infusorian drug in preparation |
| CN106038682A (en) * | 2016-07-18 | 2016-10-26 | 暨南大学 | Chinese herbal medicine compound preparation for preventing and treating chthyophthirius multifilis for fish as well as preparation and application of Chinese herbal medicine compound preparation |
| KR101769074B1 (en) * | 2017-03-30 | 2017-08-17 | 허운덕 | Compositions for repelling fur and feather, and vermin comprising sophora extract as active ingredients |
-
2018
- 2018-01-29 CA CA3088618A patent/CA3088618C/en active Active
- 2018-01-29 GB GB2010954.2A patent/GB2583664B/en active Active
- 2018-01-29 WO PCT/KR2018/001249 patent/WO2019146826A1/en active Application Filing
- 2018-01-29 NO NO20181070A patent/NO345471B1/en unknown
Patent Citations (3)
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| CN101040936A (en) * | 2007-04-28 | 2007-09-26 | 大连水产学院 | Compound Chinese traditional medicine agent for preventing fish verminosis |
| CN101690767A (en) * | 2009-09-29 | 2010-04-07 | 北京大北农动物保健科技有限责任公司 | Compound pesticide for fishing and preparation method and application thereof |
| CN105029014A (en) * | 2015-07-08 | 2015-11-11 | 滁州市南谯区瑞琳水产养殖专业合作社 | Pine needle soft feed for reducing fish dermatopathy and preparation method thereof |
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Also Published As
| Publication number | Publication date |
|---|---|
| CA3088618C (en) | 2023-10-03 |
| GB2583664A (en) | 2020-11-04 |
| GB202010954D0 (en) | 2020-09-02 |
| GB2583664B (en) | 2022-09-14 |
| CA3088618A1 (en) | 2019-08-01 |
| WO2019146826A1 (en) | 2019-08-01 |
| NO20181070A1 (en) | 2018-08-14 |
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