KR101877845B1 - Composition for preventing or treating parasitization in fishes comprising jerusalem artichoke and sophora flavescens aiton extract - Google Patents

Abstract

The present invention relates to a composition for preventing or treating parasitization in fish, including Jerusalem artichoke and Sophora flavescens aiton extracts as active ingredients, and more particularly, to a method for preventing or treating parasitization in fish, which includes the step of directly inputting a pharmaceutical composition for preventing or treating infestation of sea lice which is a fish parasite, a feed additive composition for prevention or improvement, and the composition to the fish or a farm. Accordingly, the present invention can prevent or treat infectious disease due to the fish parasite without tolerance, toxicity, and environment pollution.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing or treating a fish parasitic infection, which comprises a potato extract, a potato extract, and a ginseng extract. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for preventing or treating fish parasitic infection,

The present invention relates to a composition for preventing or treating a parasitic infectious disease of fish comprising an extract of Pork Potato (Jerusalem artichoke) and Gosam (Sophora flavescens Aiton) as an active ingredient. More specifically, , A method for preventing or treating parasitic infectious diseases of fish, comprising the step of feeding the composition directly to a fish or a farm, .

Worldwide, fish aquaculture is an alternative to depletion of fishery resources and is mainly focused on fish species with excellent commercial characteristics. Among them, salmon contains many unsaturated fatty acids such as DHA and EPA, The world's largest fish species, the world's largest salmon aquaculture country is Norway, followed by Britain, Chile, Canada and Australia. The common feature of salmon cultivation stations is that they are located in the waters where cold seawater flows. The global salmon market is estimated at 4.3 million tons and 20 trillion won a year, including around 60 trillion won if it includes the processing market.

Cherry salmon is a salmon inhabited by rivers and oceans of Korea, China, Japan and Russia. Its size is 60 cm smaller than 1 meter of Norwegian salmon. We call it Shima salmon which is Japanese name. In other words, in Korea, salmon and trout form, but they do not use the name salmon but chuck salmon (trout). Trout fishing and festivals in winter are famous. In Korea, the development of freshwater-based inland water trout farming has developed, and in 2015 it was the 50th anniversary of trout farming. Meanwhile, salmon can be classified into Pacific salmon and Atlantic salmon. In Korea, silver salmon belonging to Pacific salmon is being tried. On the other hand, Atlantic salmon is being cultivated in large scale in North Sea fjord such as Norway, and it is cultivated in Chile and Canada in the Pacific coast. Korea is still under trial research.

Marine Harvest, Norway's most known form of salmon, has succeeded in salmon farming in the 1960s, and small businesses with local fishermen have grown into a global company today. Currently, the largest salmon farming company in the world, it hatches 100 million salmon eggs a year and earns about 3 trillion won in salmon style.

However, the most problematic of the aquaculture industry is the mortality rate, which is more than 20% per year. The main cause of this is the incidence of intractable diseases and infection (viral, bacterial, parasitic) Especially in a farm where breeding density is high, "sea lice" is essential. Salmon with seaweed causes various side effects by physical and chemical damage. 'SeaIye' is a parasitic larva that is parasitic to Atlantic salmon cultured in Norway, Scotland and Canada. Once a sea bug occurs, it can spread explosively. Specifically, sea lice for fish are external parasites that live on the mucus, epidermal tissue or blood of the host fish. Sea lice are parasitic on the fish, which causes the fish to multiply, inflammation, pathogens, etc., and the mortality rate increases rapidly. There is a serious problem that the population of fish is reduced. In Norway and Scotland, there are cases where salmon are killed by 30 to 40% because fish are concentrated and cultivated intensively in the cage farm.

In the past, aquaculturists tried to solve this problem by putting insecticides on feed for salmon farming, but recently, marine species resistant to this chemical have appeared. Currently, Marine Habitus is trying to reduce the number of seas by introducing fish that feed on the sea lice naturally called feeder fish. Experts predict that marine extermination will be possible with fusion technologies including new management strategies such as insecticides and new management strategies such as genetic resistance culture, but the results have not been successful yet, and the 'emamectin benzoate' The use of insecticides containing only insecticides is the most effective means.

KR 1020010109335 A

The present invention relates to a composition for prevention or treatment of infectious diseases caused by fish parasites including environmentally friendly sea lice without causing tolerance, toxicity and environmental pollution by using pig potatoes and ginseng which are harmless to human body .

It is another object of the present invention to control, insect and eliminate fish parasites by using a feed additive composition comprising an active ingredient of a potato and a ginseng extract.

The present invention provides a pharmaceutical composition for preventing or treating a fish parasitic infectious disease, which comprises a mixture of Jerusalem artichoke and Sophora flavescens Aiton extract as an active ingredient. The pig potato extract may be leaves, stems, roots, fruits or pans of a potato potato, and the gossam extract may be leaf, stem, root, flower,

The mixture may be prepared by mixing the potato extract of Pig and the extract of Gossam gum at a weight ratio of 1:10 to 10: 1, preferably 1: 5 to 5: 1. The daily intake is suitably from 10 to 1000 mg / kg, preferably from 20 mg to 800 mg / kg.

In the present invention, the fish parasite is selected from the group consisting of sea lice, Cymothoa Exigua, Ichthyophthirius multifiliis, Bivagina tai, Anisakiasis, Distoma, Scutica, Benedenia or Anisakis Simplex, preferably in the genus Lepeophtheirus or caligus in the order Caligidae, and preferably in the genus Lepidoptera, It is preferably sea lice, Lepeoptheirus salmonis or caligus rogercresseyi.

In the present invention, fish can be collectively referred to as any fish to which the parasitic infectious disease according to the present invention can develop without limitation, and preferably salmon, trout, catfish, perch, tuna, halibut, arctic fish, sturgeon, It includes a lot of fish, sandfish, tiger prawn, carp, tilapia, rower, eel, sea bream, yellow tail, amber jacquard, jambar, rockfish, red sea bream, red sea bream, flounder, mackerel, . It is also preferably, but not exclusively, aquaculture. It is preferably Salmonidae.

The present invention also provides a feed additive composition for preventing or improving a fish parasitic infection comprising a mixture of Jerusalem artichoke and Sophora flavescens Aiton extract as an active ingredient.

The present invention also provides a composition for controlling or insecting a fish parasite, comprising a mixture of Jerusalem artichoke and Sophora flavescens Aiton extract as an active ingredient.

As another example, the present invention provides a method for preventing or treating parasitic infestation of fish by injecting a composition comprising a mixture of Jerusalem artichoke and Sophora flavescens Aiton extract as an active ingredient into fish.

The composition comprising the mixture of the potatoes and the extract of Piglets of the present invention as an active ingredient is useful as a preventive or therapeutic agent for fish parasites because it prevents the sea lice or prevents the access of the parasites when consumed by the fish .

Figure 1 shows the death, blunting, and survival rate of sea lice in a concentration-dependent manner.
Figure 2 shows the experimental conditions for sea lice in vivo.
Fig. 3 shows the iv vio results for the mixture after feeding a mixture of pig potato and ginseng extract for about one week while feeding the feed with a stabilization period and infecting the sea lice.
Figure 4 shows the iv vio result for a sea of a mixture of porcine potato and ginseng extract under the experimental conditions without the stabilization period.

Jerusalem artichoke is also known as ground potato, sorghum, mackerel, mung bean, mulberry, potato, kukwoo and mackerel. It has excellent effect on prevention of diabetes treatment and complication in root, Or more. It is also used as animal feed, fructose production, and ethanol. Especially, as it is known to be good for diabetes, farmers who pick up, grow and sell natural products are increasing, and they are widely used as health functional foods. It is also known that pig potatoes have no toxic components.

Gosam is a perennial plant belonging to the rosemary bean family of dicotyledonous plants. It is a wild flower native to the mountain areas of the country. The scientific name of Gosam is Sophorae flavescens Aiton, and the name Gosam uses the letter "Taste" and the word "Gosam". It mainly uses roots as medicinal materials, and it has the action of giant wind insecticide (风风 殺虫), 清热 ino (淸 热 尿 尿), and cheongryungmushu (淸 热 燥濕) to treat leukocytopenia and anti-radiation action, Tumor, antimicrobial, and immunosuppression.

In the present invention, porcine potatoes and ginseng can be used as a living organism, or as a dried product using a drying method such as natural drying, oven drying, hot air drying, freeze drying, or the like, And gosam can be used

The term "extract" in the present invention means an extract obtained by the extraction treatment of the above-mentioned porcine potato or bamboo shoot, a diluted solution or concentrate of the extract, a dried product obtained by drying the extract, a controlled preparation or a purified product of the extract, , The extract itself and extracts of all the formulations that can be formed using the extract.

In the porcine or ginseng extract of the present invention, the method for extracting the porcine potato and the ginseng is not particularly limited and may be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.

In the present invention, the kind of the extraction solvent used for extracting the potatoes and the ginseng is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof. Water, a lower alcohol, 1,3-butyleneglycol, and ethyl acetate may be used alone or in admixture of two or more. In the present invention, the extract may be extracted with a solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms and a mixed solvent thereof, and the alcohol may be methanol, ethanol, propyl alcohol or butanol, But is not limited to.

In the present invention, a method for obtaining a porcine potato and a ginseng extract is more preferably a method in which the potato and the ginseng are naturally dried for 5 to 7 days at room temperature after harvesting, and pulverized to about 1 to 20 times, preferably about (C1) to 4 (C4) lower alcohols such as methanol, ethanol and butanol, or a mixed solvent thereof having a mixing ratio of about 1: 0.1 to 1:10 Extraction extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction for about 12 to 6 days, preferably 5 days, at an extraction temperature of 20 to 100 ° C, preferably at room temperature Can be extracted using the extraction method of < RTI ID = 0.0 >

In the present invention, the compounds contained in the potato extract include Inuline, Betaine, Lutein, Helenien, hemagglutinin and saponin. Such compounds may be obtained from the extract or fraction by conventional separation and purification processes.

In the present invention, the compounds contained in the extract of Gossam gum extract include matrine, oxymatrine, anagyrine, sophoranol, cytisine, methylcytisine, xanthohumol, noranhydroicaritin, isoanhydroicaritin, glucoluteolin, kaempferol-3-sophoroside, and the like. Heilsteine, Heilsteine-4-rhamnosyl glucoside, etc. These compounds can be obtained from the extract or the fraction by a conventional separation and purification process.

In the present invention, the fish may be any fish which can cause parasitic infectious diseases according to the present invention without limitation, and preferably includes salmon, trout, catfish, perch, tuna, halibut, arctic fish, sturgeon, Includes prawns, staves, westerns, carp, tilapia, lobsters, eels, sea bream, yellow tail, amberjack, jambari, rockfish, red sea bream, red sea bream, flounder, mackerel, can do. It is also preferably, but not exclusively, aquaculture. In one embodiment of the present invention, an experiment was conducted using a representative salmon

The parasite of the present invention collectively refers to an invertebrate living in parasitic life outside and in the body of another organism (fish, crustacean, animal, plant, etc.). In detail, the sea lice, Cymothoa Exigua, Ichthyophthirius multifiliis, Bivagina tai, Anisakiasis, Distoma, Tapeworm, Scutica, Benedenia, Anisakis Simplex, and the like. It also includes at least one of internal parasites (protozoa, annular animals, linear animals, etc.) and external parasites (flat animals, arthropods, etc.).

In one embodiment of the present invention, it has been confirmed that the mixture of the pig potato and the ginseng extract has an effect of exterminating sea lice. Therefore, it is possible to prevent the parasite infection of fish including a mixture of the potato extract and the ginseng extract of the present invention, Improvement or therapeutic uses.

In the present invention, the term "prevent" means any action that inhibits or slows the onset of parasitic infectious disease of fish by the composition, and "treatment" means that the composition of the fish, Means any action that is changed.

The term "pharmaceutical composition" in the present invention means a preparation for the purpose of prevention or treatment of disease, and may be formulated into various forms according to ordinary methods. For example, it can be formulated into oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions and syrups, and can be formulated in the form of external preparations, suppositories, and sterilized injection solutions. However, it is most preferable that the composition of the present invention is provided in the form of a bathing agent for the purpose of treating diseases of fishes.

In addition, each of the formulations may be further prepared by incorporating pharmaceutically acceptable carriers such as buffers, preserving agents, wetting agents, solubilizing agents, isotonic agents, stabilizers, bases, excipients, lubricants and the like in the art .

Meanwhile, the pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. The term "pharmaceutically effective amount " of the present invention means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to veterinary treatment and not causing side effects, , The severity, the activity of the drug, the sensitivity to the drug, the method of administration, the time of administration, the route of administration and the rate of release, the duration of the treatment, the factors including the combined or concurrently used drugs, and other factors well known in the veterinary arts.

In another aspect, the present invention provides a feed additive composition for preventing or ameliorating a parasitic infestation disease of fish, comprising a mixture of a potato and a ginseng extract as an active ingredient.

For the purpose of the present invention, a mixture of porcine potato and ginseng extract can be added to the feed composition as a feed additive, as it can be used for the purpose of preventing or ameliorating the parasitic infestation of fish.

In the present invention, the feed additive is collectively referred to as a substance added to the feed in a nutritive or special manner for a specific purpose. In the present invention, it means a substance added for the purpose of preventing or improving a parasitic infectious disease of a fish. The mixture of porcine potato and ginseng extract of the present invention may be included in the feed composition as the feed additive, and the feed may be used to collectively mean all the foods supplied to the fish.

The feed composition of the present invention may further contain a binder, an emulsifying agent, a preservative, and the like added to prevent quality deterioration. The feed composition may contain an amino acid, a vitamin, an enzyme, a probiotics, a flavoring agent, A compound, a silicate, a buffer, a coloring agent, an extracting agent, an oligosaccharide, and the like, and may further include, but is not limited to, a feed mixture.

The compound feed commonly used in the feed composition for fish includes any one or more protein sources selected from fish meal, casein, bone meal, and shrimp powder, or any one or more carbohydrate sources selected from sweet potato starch, potato starch, wheat starch, But the present invention is not limited thereto. Even if it is used in combination with the compound feed, it is possible to prevent or ameliorate parasitic infectious diseases according to the present invention, The effect is the same.

Another aspect of the present invention provides a method for preventing or treating parasitic infestation in a fish, comprising the step of administering a composition comprising a mixture of a potato and a ginseng extract as an active ingredient directly to a fish or to a farm.

As another embodiment, there is provided a method for preventing or treating parasitic infestation of fish, comprising the step of administering a pharmaceutical composition or feed additive composition comprising the above-mentioned mixture of potato and ginseng extract as an active ingredient directly or in a fish farm .

The method for preventing or treating a parasitic infectious disease of a fish may include any step in which the composition of the present invention is directly or indirectly delivered to a fish by including the step of administering the fish directly or in a farm.

Specifically, for example, the purpose of the present invention may be accomplished by directly injecting or feeding fish, and may also be accomplished by means of bathing or eating. Preferably, the effective concentration of the composition may be coated on the feed and fed to fish having lesions due to parasitic infectious disease, but is not limited thereto. At this time, the dosage of the composition according to the present invention may be varied depending on the weight of the fish to be treated, the health condition, the administration time, and the severity of the disease.

Hereinafter, effects of the present invention will be described by way of examples.

[ Example  1]: preparation of pig potato extract

Pork potatoes were thoroughly washed with water, allowed to dry naturally for 5-7 days, and then cut. 1.2 kg of methanol was added to 50 kg of cut pork potato, and the mixture was extracted at room temperature for 5 days and then filtered. It was concentrated under reduced pressure, lyophilized and powdered.

[ Example  2] : GoSam  Preparation of extract

The roots were thoroughly washed with water, naturally dried for 5 to 7 days, and then cut. Three times as much methanol as weight was added to 50 kg of the cut gosam, and the mixture was extracted at room temperature for 5 days and then filtered. It was concentrated under reduced pressure, lyophilized and powdered.

The pig potato extract prepared in Example 1 and Example 2 and the ginseng extract were mixed at a weight ratio of 1: 1 and used for in vivo experiments.

[ Example  3] Dependence of the concentration of sea lice on the mixture of potato and ginseng extracts,

Experiments were conducted using a 24-well plate to confirm the effectiveness of the sea lice eradication by using a mixture of the potatoes and the ginseng extract, which were confirmed to have a repelling effect on the sea lice. All the solutions used in the experiment were kept at 10 ° C during the experiment. After incubation for 24 hours, sea lice were added to each well. The effect of sea lice eradication was confirmed by observing the condition.

As shown in FIG. 1 (a), about 75% of the sea lice are alive in the case of the control without any treatment. However, as the concentration of the mixture of the potato and the ginseng extract is increased, 1 (b) shows that when the concentration of the mixture (effective substance) of the potato and ginseng extract is 0.54 mg / ml, the number of dead sea lice is 50% (LD50 ) Was deadly. And sea lice population 50% (EC50) at 0.03 mg / ml became moribund state or swimming was inhibited and showed excellent effect on sea lice.

[ Example  4]: Mixture of in vivo  Experiment

About 30g of salmon per 100g of salmon was added per 200L tank (4 sections x 3 tanks = 12 tanks; control, 50mg / kg, 150mg / kg, 500mg / kg) Was applied to the feed for 7 days, and then sea lice were infused with sea lice (67 lbs) per se. Thereafter, the salmon was uniformly collected from each of 12 tanks at intervals of 10 days, 17 days and 23 days to confirm the degree of sea lice infection (see FIG. 2).

As a result, there was no difference between group A and group A, which was supplemented with a mixture of pig potato and ginseng extract. After 17 days of treatment, the infection rate of sea lice was significantly decreased with increasing dose. In group B, which had been treated with a mixture of pig and persimmon extracts at the lowest concentration 23 days after the administration of the substance, the infection by sea lice was greatly reduced and it was effective in eradicating sea lice. These results indicate that a mixture of the potato and ginseng extracts has an effect on sea lice eradication in a concentration-dependent manner over time (see Figure 3)

In addition, the sea lice are divided into planktonic and parasitic life span with time, and they are different from each other depending on the water temperature, but they take 17 ~ 72 days to become adult. They attach to the host and attach to the host It starts to eat food at the Copepodid, and continues to damage the salmon through the Chalimus until it becomes an adult. In the present invention, experiments were started by administering sea lice of Copepodid stage, and the effect of sea lice eradication on the mixture of potato and gosam was observed until adult. The sea lice are more difficult to eradicate as they grow into adults, but the mixture of the present invention prevents the damage of sea lice from the time of Copepodid until becoming adult, , And has an amazing effect of treating.

[ Example  5]: in in a condition without stabilization period vivo  Experiment

About 50-70 g of salmon was put into a 150 L tank (150 mg / kg) and about 20 salmon were put into the tank and 100 sea lice per a salmon were infused for sea lice infection. Pork potatoes and ginseng extracts were coated for 10 days, and salmon was collected constantly to determine the level of sea lice infection. As a result, the infection rate of sea lice was significantly reduced to about 66% after 10 days of administration, although no difference was observed between group A and group A. The results showed that a mixture of potato and ginseng extracts was effective in eradicating sea lice in a short time (see Figure 4)

In conclusion, the mixture of porcine potato and ginseng extract of the present invention has anti-sea lice activity, so that it can be used for the prevention or treatment of parasitic infectious diseases of fish, for the prevention of parasites, and for control thereof.

Claims (10)

A pharmaceutical composition for preventing or treating a fish parasitic infectious disease of the genus Caligidae comprising a mixture of Jerusalem artichoke and Sophora flavescens Aiton extract as an active ingredient
[Claim 2] The pharmaceutical composition according to claim 1, wherein the mixture is a mixture of a potato extract and a gossam extract in a weight ratio of 1: 10 to 10:
The pharmaceutical composition according to claim 1, wherein the fish parasite is a sea lice.
delete The pharmaceutical composition according to claim 1, wherein the fish parasite is genus Lepeophtheirus or genus Caligus.
6. The pharmaceutical composition according to claim 5, wherein the fish parasite is Lepeoptheirus salmonis or caligus rogercresseyi.
The pharmaceutical composition according to claim 1, wherein the fish is Salmonidae.
A feed additive composition for preventing or ameliorating a fish parasitic infestation of a fish (caligidae) comprising a mixture of Jerusalem artichoke and Sophora flavescens Aiton extract as an active ingredient
A composition for controlling fish parasites or an insecticidal composition comprising a mixture of Jerusalem artichoke and Sophora flavescens Aiton extract as active ingredients
A method of preventing or treating a fish parasite infestation by feeding a composition comprising a mixture of Jerusalem artichoke and Sophora flavescens Aiton extract as an active ingredient into fish

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