KR101769074B1 - Compositions for repelling fur and feather, and vermin comprising sophora extract as active ingredients - Google Patents
Compositions for repelling fur and feather, and vermin comprising sophora extract as active ingredients Download PDFInfo
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- A01N63/04—
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- Y10S514/919—
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Abstract
Description
본 발명은 고삼추출물을 포함하는 유해조수 및 해충 기피제 조성물 및 그의 제조방법에 관한 것이다. The present invention relates to harmful tidal water and insect repellent compositions comprising a ginseng extract and a process for preparing the same.
작물은 사람의 보호 및 관리 하에서 군락을 이루고 생육하는 식물을 의미하는데, 각종 채소류와 같은 식용작물 외에 꽃 등의 관상작물도 포함된다.Crops means plants that grow and grow in a community under the protection and management of humans, including corn crops such as flowers as well as edible crops such as various vegetables.
작물은 노지뿐만 아니라, 출하시기를 조절하기 위하여 비닐 하우스와 같은 시설에서 재배되는데, 작물의 생산량 증대 및 품질 향상을 위하여 해충 방제를 위하여 각종 농약이 사용되고 있다.The crops are cultivated in facilities such as vinyl houses to regulate the release date as well as the paddy fields. Various pesticides are used for the control of pests to increase the production of crops and improve the quality.
그러나 상기와 같은 농약의 사용은 방제자 및 소비자 등의 인체 및 환경에 악영향을 미치며, 농약에 대한 해충의 내성 증가시킴으로써 잠재해충의 주요 해충화, 방제비용의 증가 등과 같은 문제점들을 야기한다. However, the use of the agricultural chemicals as described above adversely affects the human body and environment such as disinfectants and consumers, and increases the resistance of insect pests to pesticides, thereby causing problems such as major insect pest insects and an increase in control costs.
최근 들어 농약이 잔류될 가능성이 있는 오이, 토마토, 고추 등의 식용 과채류에 대한 소비자들의 소비회피 경향도 나타나고 있는 것으로 보고되고 있다. Recently, it has been reported that consumers tend to avoid consumption of edible fruits and vegetables such as cucumber, tomatoes and peppers, which are likely to contain pesticides.
상기와 같은 문제를 해결하기 위하여 다양한 천연 살충성분의 농약을 사용하려는 시도가 계속되고 있으나, 살충 효과에 한계가 있고 방제비용이 과다하게 소요되는 등의 문제점 때문에 현실화되지 못하고 있는 실정이다.In order to solve the above problems, various attempts have been made to use pesticides having natural insecticidal properties, but they have not been realized due to problems such as insecticidal effect and excessive control cost.
본 발명은, 종래기술의 상기와 같은 단점을 해결하기 위하여 안출된 것으로서,SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned disadvantages of the prior art,
환경에 대한 피해가 적고 인체에 해가 없으며, 살충력이 우수한 천연 살충제 조성물 및 그의 제조방법을 제공하는 것을 목적으로 한다.It is an object of the present invention to provide a natural insecticide composition which is less harmful to the environment, is harmless to the human body, and has excellent insecticidal power, and a method for producing the same.
본 발명은, 상기 목적을 달성하기 위하여, The present invention, in order to achieve the above object,
고삼 뿌리 10~20 중량부, 창포 5~10 중량부, 및 조릿대잎 5~10 중량부에 클로로포름, 저급알코올 또는 이들의 혼합물 300~600 중량부를 가하여 5~24시간 동안 추출 및 농축하고, 상기 농축액에 바실러스 투린지엔시스(Bacillus thuringiensis) 균주를 3~5 중량부를 접종하고 배양하여 제조된 제1살충성분 30~70 중량%; 및10 to 20 parts by weight of ginseng roots, 5 to 10 parts by weight of iris, 5 to 10 parts by weight of sage leaves, 300 to 600 parts by weight of chloroform, a lower alcohol or a mixture thereof is added and the mixture is extracted and concentrated for 5 to 24 hours. 30 to 70% by weight of a first insecticide component prepared by inoculating and culturing 3 to 5 parts by weight of a strain of Bacillus thuringiensis; And
작두콩 10~20 중량부, 흰목이버섯 2~5 중량부, 및 삼지구엽초 5~10 중량부에 물 300~600 중량부를 가하여 열수추출 및 농축하고, 상기 농축액에 바실러스 섭틸리스(Bacillus subtilis)균주를 3~5 중량부를 접종하고 배양하여 제조된 제2살충성분 30~70 중량%;를 포함하는 천연 살충제 조성물을 제공한다.(Bacillus subtilis) is added to the concentrate by addition of 300 to 600 parts by weight of water to 10 to 20 parts by weight of soybean curd, 2 to 5 parts by weight of white mushroom, and 5 to 10 parts by weight of three- And 30 to 70% by weight of a second insecticide component prepared by inoculating and culturing 3 to 5 parts by weight of the insecticidal composition.
또한, 본 발명은In addition,
고삼 뿌리 10~20 중량부, 창포 5~10 중량부, 및 조릿대잎 5~10 중량부에 클로로포름, 저급알코올 또는 이들의 혼합물 300~600 중량부를 가하여 5~24시간 동안 추출 및 농축하는 단계;Adding 5 to 10 parts by weight of ginseng roots, 5 to 10 parts by weight of iris, 5 to 10 parts by weight of sage leaves, and adding 300 to 600 parts by weight of chloroform, a lower alcohol or a mixture thereof to extraction and concentration for 5 to 24 hours;
상기 농축액에 바실러스 투린지엔시스(Bacillus thuringiensis) 균주를 3~5 중량부를 접종하고 배양하여 제1살충성분을 제조하는 단계;3 to 5 parts by weight of a strain of Bacillus thuringiensis is inoculated into the concentrate and cultured to prepare a first insecticidal component;
작두콩 10~20 중량부, 흰목이버섯 2~5 중량부, 및 삼지구엽초 5~10 중량부에 물 300~600 중량부를 가하여 열수추출 및 농축하는 단계;Extracting and concentrating water by adding 300 to 600 parts by weight of water to 10 to 20 parts by weight of soybean curd, 2 to 5 parts by weight of white mushroom, and 5 to 10 parts by weight of three-leafed mushroom;
상기 농축액에 바실러스 섭틸리스(Bacillus subtilis) 균주를 3~5 중량부를 접종하고 배양하여 제2살충성분을 제조하는 단계; 및3 to 5 parts by weight of a strain of Bacillus subtilis is inoculated into the concentrate and cultured to prepare a second insecticidal component; And
상기 제1살충성분 및 제2살충성분을 3:7~7:3의 중량비로 혼합하는 단계;를 포함하는 천연 살충제 조성물의 제조방법을 제공한다.Mixing the first insecticidal component and the second insecticidal component in a weight ratio of 3: 7 to 7: 3.
본 발명의 환경에 대한 피해가 적고 인체에 해가 없으며, 살충력이 우수한 천연 살충제 조성물 및 그의 제조방법을 제공한다. The present invention provides a natural insecticidal composition having less damage to the environment, harmless to the human body, and excellent insecticidal power, and a method for producing the same.
이하에서 본 발명을 자세히 설명한다.Hereinafter, the present invention will be described in detail.
고삼 뿌리 10~20 중량부, 창포 5~10 중량부, 및 조릿대잎 5~10 중량부에 클로로포름, 저급알코올 또는 이들의 혼합물 300~600 중량부를 가하여 5~24시간 동안 추출 및 농축하고, 상기 농축액에 바실러스 투린지엔시스(Bacillus thuringiensis) 균주를 3~5 중량부를 접종하고 배양하여 제조된 제1살충성분 30~70 중량%; 및10 to 20 parts by weight of ginseng roots, 5 to 10 parts by weight of iris, 5 to 10 parts by weight of sage leaves, 300 to 600 parts by weight of chloroform, a lower alcohol or a mixture thereof is added and the mixture is extracted and concentrated for 5 to 24 hours. 30 to 70% by weight of a first insecticide component prepared by inoculating and culturing 3 to 5 parts by weight of a strain of Bacillus thuringiensis; And
작두콩 10~20 중량부, 흰목이버섯 2~5 중량부, 및 삼지구엽초 5~10 중량부에 물 300~600 중량부를 가하여 열수추출 및 농축하고, 상기 농축액에 바실러스 섭틸리스(Bacillus subtilis)균주를 3~5 중량부를 접종하고 배양하여 제조된 제2살충성분 30~70 중량%;를 포함하는 천연 살충제 조성물에 관한 것이다.(Bacillus subtilis) is added to the concentrate by addition of 300 to 600 parts by weight of water to 10 to 20 parts by weight of soybean curd, 2 to 5 parts by weight of white mushroom, and 5 to 10 parts by weight of three- And 30 to 70% by weight of a second insecticide component prepared by inoculating and culturing 3 to 5 parts by weight of a pesticidal composition.
상기 제1살충성분은 추출시에 으름 5~10 중량부를 더 첨가하여 제조되고, 상기 제2살충성분은 추출시에 파극천 5~10 중량부를 더 첨가하여 제조될 수 있으며,그 경우에 더 우수한 살충효과를 제공한다. The first insecticidal component may be prepared by further adding 5-10 parts by weight of the insecticidal component at the time of extraction, and the second insecticidal component may be prepared by further adding 5-10 parts by weight of the insecticidal compound at the time of extraction. In this case, Effect.
상기 천연 살충제 조성물은 벼멸구, 배추좀나방, 점박이응애, 담배거세미나방, 총채벌레, 굴파리, 온실가루이, 청벌레, 파밤나방, 심식나방, 민달팽이, 진딧물, 벼물바구미, 이화명나방, 혹명나방 중 선택된 1종 이상의 해충 방제에 사용될 수 있으며, 특히 진딧물의 살충에 바람직하게 사용될 수 있다. The natural insecticide composition is selected from the group consisting of brown planthopper, cabbage moth, spotted mite, tobacco spider moth, thrips worm, gullfruit, greenhouse powder, cilbrot, parakeet moth, moth moth, aphid moth, weevil moth, It can be used for pest control more than species, and can be suitably used especially for the insecticide of aphids.
이하에서 상기 천연 살충제 조성물물의 성분에 대하여 자세히 설명한다.The components of the natural pesticide composition will be described in detail below.
상기 고삼은 콩과식물로서, 항알레르기 활성이 크며, 이뇨작용, 자궁수축작용, 해열, 진통, 살충효과 등이 크다고 알려져 있다.The ginseng is a soybean plant and has a high antiallergic activity, and is known to have a great effect on diuretic action, uterine contraction, antipyretic, analgesic and insecticidal effects.
상기 창포는 약리실험에서 진정, 진통, 항경련 작용과 순환계통에 대한 작용, 진해거담 작용, 소화기에 대한 작용, 피부 미용에 관한 작용이 보고되어 있다. 본 발명자들은 이 밖에 살균 및 살충에도 효과가 있음을 확인하였다.The iris has been reported to have effects on calming, analgesic, anticonvulsant and circulatory system, action on the digestive tract, action on the digestive system, and skin care in pharmacological experiments. The inventors of the present invention have also confirmed that they are effective also for sterilization and insecticide.
상기 조릿대잎은 천연 식이 유황성분을 포함하며, 항암, 기침을 멎게 하는 작용, 염증, 해독, 진정, 진통, 이뇨작용을 갖는 것으로 알려져 있다. 본 발명자들은 이 밖에 살균 및 살충에도 효과가 있음을 확인하였다.The bamboo leaves contain natural dietary sulfur components and are known to have anti-cancer, cough-stopping action, inflammation, detoxification, sedation, analgesia and diuretic action. The inventors of the present invention have also confirmed that they are effective also for sterilization and insecticide.
상기 작두콩은 본초강목에서 장과 위를 보호 하고 속을 따뜻하게 하고 신장기능을 강화하고 원기를 보호하고 종기나 치질 화농성 염증 질환에 효과가 있다고 언급되어 있다. 작두콩은 맛이 달고 그 성질은 따뜻하며, 비염, 알레르기성 비염, 감기, 축농증, 콧물, 재채기, 코막힘, 천식 등의 호흡기 질환에 효가가 탁월한 것으로 알려져 있다. 또한, 간이나 종기 등에 대한 염증에 탁월하고 구강염 중이염 등에도 좋으며, 혈액순환을 촉진시켜 주며, 면역력을 강화시키고, 체내 독소 배출 및 독성 해독 작용을 갖는 것으로 알려져 있다. 특히, 껍질 채로 건조한 작두콩이 더욱 바람직하게 사용될 수 있다. 본 발명자들은 이 밖에 살균 및 살충에도 효과가 있음을 확인하였다.The soy sauce is said to protect the intestines from stomach gills, warm the genus, enhance the kidney function, protect the scalp and be effective against inflammation of the boils and hemorrhoids. It is known that soybean is excellent for respiratory diseases such as rhinitis, allergic rhinitis, cold, sinusitis, runny nose, sneezing, nasal obstruction and asthma. In addition, it is excellent for inflammation of liver and boil, and is good for oral mucositis, etc. It is known that it promotes blood circulation, strengthens immunity, excretes toxins in the body and detoxifies. Particularly, dried soy sauce which is dried in shell may be more preferably used. The inventors of the present invention have also confirmed that they are effective also for sterilization and insecticide.
상기 흰목이버섯(Tremella fuciformis)은 여름과 가을에 각종 활엽수의 죽은 나무 또는 나뭇가지에서 자란다. 자실체는 지름 3~8cm, 높이 2~5cm, 불규칙한 파형의 덩어리 모양 겹꽃 모양이다. 표면은 순백색, 평활하며, 자실층은 표면 전체에 발달한다. 조직은 반투명한 젤라틴질이다. 식용 버섯으로 면역 증진, 콜레스테롤 저하, 혈당강하, 항산화 작용이 있는 약용 버섯이며, 한방에서는 호흡기 질환, 위염, 고혈압, 월경 불순 등 여러 질환에 도움이 된다고 알려져 있다. 주요 성분으로는 비타민 B1, B2, D 및 니아신(niacin), 만니톨(mannitol), 글리세롤(glycerol), 글루코스(glucose), 트레할로스(trehalose), 헤미셀룰로우스(hemicellulose), 키틴(chitin), 펙틴(pectin), 루코노실로 만난(luconoxylomannan), 리그닌(lignin) 등이 알려져 있다. 본 발명자들은 이 밖에 살균 및 살충에도 효과가 있음을 확인하였다.The above-mentioned Tremella fuciformis grows in various hardwood dead trees or branches in summer and autumn. Fruiting body is 3 ~ 8cm in diameter, 2 ~ 5cm in height, lumpy shape with irregular wave shape. The surface is pure white, smooth, and the fascial layer develops throughout the surface. The tissue is translucent gelatinous. It is medicinal mushroom which has immunity enhancement, cholesterol lowering, blood sugar lowering, antioxidant effect by edible mushroom, and it is known that it is helpful in various diseases such as respiratory diseases, gastritis, hypertension and menstrual irregularity. The main components include vitamins B1, B2, D and niacin, mannitol, glycerol, glucose, trehalose, hemicellulose, chitin, pectin, (pectin), luconoxylomannan, lignin, and the like are known. The inventors of the present invention have also confirmed that they are effective also for sterilization and insecticide.
상기 삼지구엽초는 한방에서 ‘음양곽’이라 하여 자양강장제로 쓴다. 선령비, 방장초 등으로도 불린다. 최음, 강장, 강정, 거풍 등에 효과가 있다. 보통은 물에 달여 마시지만 술을 담가 먹어도 같은 효과를 볼 수 있다. 잎과 꽃의 모양이 독특하므로 관상용으로도 가치가 높다. 본 발명자들은 이 밖에 살균 및 살충에도 효과가 있음을 확인하였다.The above-mentioned Sansho mushroom is used as a nourishing tonic agent in a room called 'Yin Yanggu'. It is also referred to as an age ratio, a guardian candle, and so on. It has effects on aphrodisiac, gangjang, gangjeong, and giant wind. Usually, they drink in water for a few months, but you can get the same effect by soaking in alcohol. Leaves and flower shapes are unique, so they are highly valuable for ornamental purposes. The inventors of the present invention have also confirmed that they are effective also for sterilization and insecticide.
상기 으름(Akebiae quinata Decne)은 으름덩굴과에 속하는 낙엽성 덩굴식물로, 전국 각지에서 자생한다. 새순과 어린 줄기는 나물로 먹었고, 꽃은 말려두었다가 향수를 대신하여 사용되었다. 줄기의 삶은 물은 노란색이나 황갈색의 염료로 사용되었으며, 특히 소염성 이뇨제, 소변통, 요도염, 인후통, 정신신경안정제에 효능이 있어 약용으로도 중요하게 사용되었다. 뿌리, 껍질은 배뇨장애에 효능이 있으며, 열매는 이뇨제, 중풍, 관절염에 효능이 있다. 본 발명자들은 이 밖에 살균 및 살충에도 효과가 있음을 확인하였다.Akebiae quinata Decne is a deciduous vine plant belonging to the genus Lepidoptera, and grows throughout the country. Seedlings and young stems were eaten as herbs, and the flowers were dried and used instead of perfume. Stem boiled water was used as a yellow or yellowish dye, and was especially important for medicinal use because it has efficacy for anti-inflammatory diuretics, urinalysis, urethritis, sore throat, and psychotropic drugs. Roots and bark are effective for urination disorders, and fruits are effective for diuretics, paralysis, and arthritis. The inventors of the present invention have also confirmed that they are effective also for sterilization and insecticide.
상기 파극천(Morinda officinalis)은 인도에서는 인도 뽕나무, 중국에서는 [0027] 바지티안, 카리브해안에서는 진통제 나무, 오스트레일리아에서는 치즈과일, 타히티섬에서는 노노라고 한다. 동의보감에는 해파극(海巴戟) 또는 파극천(巴戟天)이라고 되어 있다. 잎, 줄기, 꽃, 열매, 씨 등이 민간요법에 사용되어 왔으며, 남태평양 지역의 고대 문헌에 의하면 최고의 천연 치료제로 기록되어 있다. 실제로 안트라퀴논, 세로토닌 등의 성분이 들어 있어 소화작용을 돕고 통증을 줄여주며 고혈압과 암 등에도 효과가 있는 것으로 밝혀졌다. 본 발명자들은 이 밖에 살균 및 살충에도 효과가 있음을 확인하였다.The above Morinda officinalis is said to be Indian mulberry in India, Vagitian in China, analgesic tree in Caribbean, cheese fruit in Australia and Nono in Tahiti. It is said to be a sea pirate or a paradise. Leaves, stems, flowers, fruits and seeds have been used in folk remedies and are listed as the best natural remedies according to ancient literature in the South Pacific. In fact, it contains anthraquinone and serotonin, which helps digestion, reduces pain, and works on hypertension and cancer. The inventors of the present invention have also confirmed that they are effective also for sterilization and insecticide.
상기 파극천 추출물은 파극천의 다양한 기관 또는 부분 즉, 잎, 꽃, 열매, 줄기, 뿌리, 가지 및 껍질등을 추출하여 얻은 것을 의미하며, 바람직하게는 파극천의 뿌리를 추출하여 얻은 것을 의미한다.The above-mentioned Porphyrae extract means obtained by extracting various organs or parts such as leaves, flowers, fruits, stems, roots, branches and bark, etc., preferably obtained by extracting roots of Phragmites.
본 발명에서 제1살충성분의 제조에 사용되는 바실러스 튜린지엔시스(Bacillus thuringiensis)로는 한국생명공학연구원 유전자은행(KCTC)에 기탁된 균주 중 KCTC 1508 또는 KCTC1509 를 분양받아 사용할 수 있다. Bacillus thuringiensis used in the production of the first insecticidal component in the present invention may be KCTC 1508 or KCTC1509, which has been deposited with KCTC (Korea Research Institute of Bioscience and Biotechnology).
본 발명에서 제2살충성분의 제조에 사용되는 바실러스 섭틸리스(Bacillus subtilis)로는 한국과학기술연구원 부설 생명공학연구소 유전자은행에 기탁된 바실러스 섭틸리스 아밀로리쿼페이션스(KCTC 8913P) 또는 바실러스 섭틸리스 EBM 13(KCTC 0984BP)를 분양받아 사용한다.Bacillus subtilis used in the production of the second insecticidal component in the present invention may be Bacillus subtilis amyloliquefaciens (KCTC 8913P) or Bacillus subtilis deposited at the gene bank of the Institute of Bioscience & EBM 13 (KCTC 0984BP) is sold and used.
또한, 본 발명은In addition,
고삼 뿌리 10~20 중량부, 창포 5~10 중량부, 및 조릿대잎 5~10 중량부에 클로로포름, 저급알코올 또는 이들의 혼합물 300~600 중량부를 가하여 5~24시간 동안 추출 및 농축하는 단계;Adding 5 to 10 parts by weight of ginseng roots, 5 to 10 parts by weight of iris, 5 to 10 parts by weight of sage leaves, and adding 300 to 600 parts by weight of chloroform, a lower alcohol or a mixture thereof to extraction and concentration for 5 to 24 hours;
상기 농축액에 바실러스 투린지엔시스(Bacillus thuringiensis) 균주를 3~5 중량부를 접종하고 배양하여 제1살충성분을 제조하는 단계;3 to 5 parts by weight of a strain of Bacillus thuringiensis is inoculated into the concentrate and cultured to prepare a first insecticidal component;
작두콩 10~20 중량부, 흰목이버섯 2~5 중량부, 및 삼지구엽초 5~10 중량부에 물 300~600 중량부를 가하여 열수추출 및 농축하는 단계;Extracting and concentrating water by adding 300 to 600 parts by weight of water to 10 to 20 parts by weight of soybean curd, 2 to 5 parts by weight of white mushroom, and 5 to 10 parts by weight of three-leafed mushroom;
상기 농축액에 바실러스 섭틸리스(Bacillus subtilis) 균주를 3~5 중량부를 접종하고 배양하여 제2살충성분을 제조하는 단계; 및3 to 5 parts by weight of a strain of Bacillus subtilis is inoculated into the concentrate and cultured to prepare a second insecticidal component; And
상기 제1살충성분 및 제2살충성분을 3:7~7:3의 중량비로 혼합하는 단계;를 포함하는 천연 살충제 조성물의 제조방법을 제공한다.Mixing the first insecticidal component and the second insecticidal component in a weight ratio of 3: 7 to 7: 3.
상기 제1살충성분은 추출단계에서 으름 5~10 중량부를 더 첨가하여 제조되고, 상기 제2살충성분은 추출단계에서 파극천 5~10 중량부를 더 첨가하여 제조될 수 있다.The first insecticidal component may be prepared by further adding 5 to 10 parts by weight of cormorant in the extraction step and the second insecticidal component may be prepared by further adding 5 to 10 parts by weight of the coral acorn in the extraction step.
상기 천연 살충제 조성물의 제조방법에 있어서, 천연 살충제 조성물에 관하여 전술된 내용은 모두 적용될 수 있다. In the method for producing the natural insecticidal composition, the above-mentioned contents regarding the natural insecticidal composition are all applicable.
상기 제1살충성분 제조단계에서 추출용매인 클로로포름, 저급알코올 또는 이들의 혼합물 중에서 저급알코올은 C1~C4의 알코올을 의미한다. 상기 용매들 중에서도 에탄올이 특히 바람직하게 사용될 수 있다.In the first insecticidal component preparation step, the lower alcohol in the extraction solvent chloroform, the lower alcohol, or a mixture thereof means the C1-C4 alcohol. Of these solvents, ethanol is particularly preferably used.
상기 제1살충성분 제조단계에서 추출은 상온에서 진행될 수 있다.In the first insecticidal component production step, the extraction may proceed at room temperature.
상기 제1살충성분 제조단계 및 제2살충성분 제조단계에서 농축은 각각 추출액 100 중량부를 기준으로 추출액이 30~60 중량부가 될 때까지 진행될 수 있다. Concentration in the first insecticidal component preparation step and the second insecticidal component preparation step may be carried out until the amount of the extract is 30 to 60 parts by weight based on 100 parts by weight of the extract.
상기 제1살충성분 제조단계 및 제2살충성분 제조단계에서 배양은 각각 20~40℃에서 48~96시간 동안 진행될 수 있다. In the first insecticidal component production step and the second insecticidal component production step, the cultivation may be carried out at 20 to 40 ° C for 48 to 96 hours, respectively.
이하에서, 실시예를 통하여 본 발명을 보다 상세히 설명한다. 그러나, 하기의 실시예는 본 발명을 더욱 구체적으로 설명하기 위한 것으로서, 본 발명의 범위가 하기의 실시예에 의하여 한정되는 것은 아니다. 하기의 실시예는 본 발명의 범위 내에서 당업자에 의해 적절히 수정, 변경될 수 있다. Hereinafter, the present invention will be described in more detail by way of examples. However, the following examples are intended to further illustrate the present invention, and the scope of the present invention is not limited by the following examples. The following examples can be appropriately modified and changed by those skilled in the art within the scope of the present invention.
실시예Example 1: 천연 살충제 조성물의 제조 1: Preparation of natural insecticide composition
건조 고삼 뿌리 분말 15 중량부, 건조 창포 7 중량부, 및 건조 조릿대잎 7 중량부에 에탄올 450 중량부를 가하여 12시간 동안 추출하고, 추출액의 부피가 60%가 될 때까지 농축하였다. 상기 농축액에 바실러스 투린지엔시스(Bacillus thuringiensis) 균주로서 KCTC 1508 4 중량부를 접종하고 30℃에서 72 시간 배양하여 제1살충성분을 제조하였다.15 parts by weight of dried ginseng root powder, 7 parts by weight of dried iris, and 7 parts by weight of dried sage leaves were added with 450 parts by weight of ethanol, followed by extraction for 12 hours and concentration until the volume of the extract became 60%. 4 parts by weight of KCTC 1508 as a strain of Bacillus thuringiensis was inoculated into the concentrate and cultured at 30 DEG C for 72 hours to prepare a first insecticidal component.
껍질째로 건조한 작두콩 15 중량부, 건조 흰목이버섯 4 중량부, 및 건조 삼지구엽초 7 중량부에 물 450 중량부를 가하여 100℃로 열수추출하고, 추출액의 부피가 60%가 될 때까지 농축하였다. 상기 농축액에 바실러스 섭틸리스(Bacillus subtilis)균주로서 바실러스 섭틸리스 아밀로리쿼페이션스(KCTC 8913P) 4 중량부를 접종하고 30℃에서 72 시간 배양하여 제2살충성분을 제조하였다.Forty parts by weight of water was added to 15 parts by weight of dried pea pods, 4 parts by weight of dried white pine mushroom, and 7 parts by weight of dried pomace mushroom, and the mixture was subjected to hot extraction at 100 ° C. and concentrated until the volume of the extract became 60%. The concentrate was inoculated with 4 parts by weight of Bacillus subtilis amyloliquefaciens (KCTC 8913P) as a Bacillus subtilis strain and cultured at 30 ° C for 72 hours to prepare a second insecticidal component.
상기 제1살충성분과 제2살충성분을 5:5로 혼합하여 천연 살충제 조성물을 제조하였다. A natural insecticide composition was prepared by mixing the first insecticidal component and the second insecticidal component in a ratio of 5: 5.
실시예Example 2: 천연 살충제 조성물의 제조 2: Preparation of natural pesticide composition
건조 고삼 뿌리 분말 15 중량부, 건조 창포 7 중량부, 건조 조릿대잎 7 중량부 및 으름 7 중량부에 에탄올 450 중량부를 가하여 12시간 동안 추출하고, 추출액의 부피가 60%가 될 때까지 농축하였다. 상기 농축액에 바실러스 투린지엔시스(Bacillus thuringiensis) 균주로서 KCTC 1508 4 중량부를 접종하고 30℃에서 72 시간 배양하여 제1살충성분을 제조하였다.15 parts by weight of dried ginseng root powder, 7 parts by weight of dried iris, 7 parts by weight of dried sage leaves and 7 parts by weight of corn were added with 450 parts by weight of ethanol, and the mixture was extracted for 12 hours and concentrated until the volume of the extract became 60%. 4 parts by weight of KCTC 1508 as a strain of Bacillus thuringiensis was inoculated into the concentrate and cultured at 30 DEG C for 72 hours to prepare a first insecticidal component.
껍질째로 건조한 작두콩 15 중량부, 건조 흰목이버섯 4 중량부, 건조 삼지구엽초 7 중량부 및 파극천 7 중량부에 물 450 중량부를 가하여 100℃로 열수추출하고, 추출액의 부피가 60%가 될 때까지 농축하였다. 상기 농축액에 바실러스 섭틸리스(Bacillus subtilis)균주로서 바실러스 섭틸리스 아밀로리쿼페이션스(KCTC 8913P) 4 중량부를 접종하고 30℃에서 72 시간 배양하여 제2살충성분을 제조하였다.To 45 parts by weight of water was added to 15 parts by weight of dried pea pods, 4 parts by weight of dried white pine mushroom, 7 parts by weight of dried pomace mushroom and 7 parts by weight of dried pomace leaves, and the mixture was subjected to hot water extraction at 100 ° C. When the volume of the extract reached 60% . The concentrate was inoculated with 4 parts by weight of Bacillus subtilis amyloliquefaciens (KCTC 8913P) as a Bacillus subtilis strain and cultured at 30 ° C for 72 hours to prepare a second insecticidal component.
상기 제1살충성분과 제2살충성분을 5:5로 혼합하여 천연 살충제 조성물을 제조하였다. A natural insecticide composition was prepared by mixing the first insecticidal component and the second insecticidal component in a ratio of 5: 5.
시험예Test Example 1: 복숭아 진딧물 방제 효과 검정 1: Test effect of peach aphid control
상기 실시예 1 및 실시예 2의 천연 살충제 조성물을 사용하여 해충 방제 효과를 검정하였다. 해충은 복숭아혹진딧물을 사용하였고, 사전 개체수 대비 살충용 조성물의 분사 후 2일 또는 3일 후의 생충수와 사충수를 확인하고, 사충율을 계산하였다. 각 실험은 3회 반복되어 그 평균값을 다음 표 2에 나타내었다. The pest control effect was tested using the natural insecticidal compositions of Examples 1 and 2 above. Peach aphid was used as a pest, and the number of freshwater and larvae after 2 or 3 days after injection of the composition for insecticidal action in comparison with the population beforehand was checked, and the percentage of larvae was calculated. Each experiment was repeated three times, and the average value thereof is shown in Table 2 below.
상기 표 1에서 보는 바와 같이, 본 발명의 실시예 1 및 실시예 2의 천연 살충제 조성물은 복숭아혹진딧물의 방제 효과가 매우 우수하였다. 특히, 제1살충성분의 제조시에 으름을 더 첨가하고, 제2살충성분의 제조시에 파극천을 더 첨가하여 제조된 것인 경우에 더욱 우수한 방제 효과를 나타냈다. As shown in Table 1, the natural insecticidal compositions of Example 1 and Example 2 of the present invention showed excellent control effect on peach aphid. Particularly, when the first insecticidal component was prepared by further adding cornstalks and the second insecticidal component was further prepared by adding a deciduous stream, a more excellent control effect was exhibited.
Claims (5)
작두콩 10~20 중량부, 흰목이버섯 2~5 중량부, 및 삼지구엽초 5~10 중량부에 물 300~600 중량부를 가하여 열수추출 및 농축하고, 상기 농축액에 바실러스 섭틸리스(Bacillus subtilis)균주를 3~5 중량부를 접종하고 배양하여 제조된 제2살충성분 30~70 중량%;를 포함하는 복숭아 진딧물 살충제 조성물.Ethanol, or a mixture thereof in an amount of 300 to 600 parts by weight is added to 10 to 20 parts by weight of root, 5 to 10 parts by weight of iris root, 5 to 10 parts by weight of iris, 5 to 10 parts by weight of a sage leaf, and the mixture is extracted and concentrated for 5 to 24 hours. 30 to 70% by weight of a first insecticide component prepared by inoculating and culturing 3 to 5 parts by weight of a strain of Bacillus thuringiensis; And
(Bacillus subtilis) is added to the concentrate by addition of 300 to 600 parts by weight of water to 10 to 20 parts by weight of soybean curd, 2 to 5 parts by weight of white mushroom, and 5 to 10 parts by weight of three- And 30 to 70% by weight of a second insecticide component prepared by inoculating and culturing 3 to 5 parts by weight of the insecticidal composition.
상기 제1살충성분은 추출시에 으름 5~10 중량부를 더 첨가하여 제조된 것이며,
상기 제2살충성분은 추출시에 파극천 5~10 중량부를 더 첨가하여 제조된 것인 것을 특징으로 하는 복숭아 진딧물 살충제 조성물.The method according to claim 1,
The first insecticidal component is prepared by further adding 5 to 10 parts by weight of corms at the time of extraction,
Wherein the second insecticidal component is prepared by further adding 5 to 10 parts by weight of a pyrethrum at the time of extraction.
상기 농축액에 바실러스 투린지엔시스(Bacillus thuringiensis) 균주를 3~5 중량부를 접종하고 배양하여 제1살충성분을 제조하는 단계;
작두콩 10~20 중량부, 흰목이버섯 2~5 중량부, 및 삼지구엽초 5~10 중량부에 물 300~600 중량부를 가하여 열수추출 및 농축하는 단계;
상기 농축액에 바실러스 섭틸리스(Bacillus subtilis) 균주를 3~5 중량부를 접종하고 배양하여 제2살충성분을 제조하는 단계; 및
상기 제1살충성분 및 제2살충성분을 3:7~7:3의 중량비로 혼합하는 단계;를 포함하는 복숭아 진딧물 살충제 조성물의 제조방법.Extracting and concentrating 5 to 24 hours by adding 300 to 600 parts by weight of chloroform, ethanol or a mixture thereof to 10 to 20 parts by weight of roots, 5 to 10 parts by weight of iris, and 5 to 10 parts by weight of sage leaves,
3 to 5 parts by weight of a strain of Bacillus thuringiensis is inoculated into the concentrate and cultured to prepare a first insecticidal component;
Extracting and concentrating water by adding 300 to 600 parts by weight of water to 10 to 20 parts by weight of soybean curd, 2 to 5 parts by weight of white mushroom, and 5 to 10 parts by weight of three-leafed mushroom;
3 to 5 parts by weight of a strain of Bacillus subtilis is inoculated into the concentrate and cultured to prepare a second insecticidal component; And
And mixing the first insecticidal component and the second insecticidal component in a weight ratio of 3: 7 to 7: 3.
상기 제1살충성분은 추출단계에서 으름 5~10 중량부를 더 첨가하여 제조되며,
상기 제2살충성분은 추출단계에서 파극천 5~10 중량부를 더 첨가하여 제조되는 것인 것을 특징으로 하는 복숭아 진딧물 살충제 조성물의 제조방법.5. The method of claim 4,
The first insecticidal component is prepared by further adding 5 to 10 parts by weight of corn in the extraction step,
Wherein the second insecticidal component is prepared by further adding 5 to 10 parts by weight of a pearl grass in the extraction step.
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Cited By (7)
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| WO2019146826A1 (en) * | 2018-01-29 | 2019-08-01 | 전진바이오팜 주식회사 | Composition for preventing or treating parasitic infections in fish, comprising sophora flavescens aiton extract or fraction thereof |
| CN110742096A (en) * | 2019-11-26 | 2020-02-04 | 余庆县玉笏春茶业有限责任公司 | Small leaf broadleaf holly leaf insect-resist agent and preparation method thereof |
| KR102155499B1 (en) * | 2019-09-06 | 2020-09-14 | 주식회사 가온아이피엠 | Insect Repellent Composition Using Sophora flavescens and Morinda officinalis and Method for Manufacturing the Same |
| KR102212590B1 (en) * | 2020-07-17 | 2021-02-05 | 주식회사 가온아이피엠 | Natural pesticide composition harmless to human body and manufacturing method thereof |
| KR102212587B1 (en) * | 2020-07-14 | 2021-02-05 | 주식회사 가온아이피엠 | Orientalis McLachlan Repellent Composition Using Sophora flavescens and Morinda officinalis and Method for Manufacturing the Same |
| KR102361490B1 (en) * | 2021-08-18 | 2022-02-14 | 이용덕 | Eco-friendly pest insecticide composition for livestock and method for controlling pests by using the same |
| KR20240024438A (en) | 2022-08-17 | 2024-02-26 | 국립안동대학교 산학협력단 | Wax-type repellent composition against Thrips using alarm pheromone and control method of Thrips using it |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019146826A1 (en) * | 2018-01-29 | 2019-08-01 | 전진바이오팜 주식회사 | Composition for preventing or treating parasitic infections in fish, comprising sophora flavescens aiton extract or fraction thereof |
| GB2583664A (en) * | 2018-01-29 | 2020-11-04 | Jeonjin Bio Co Ltd | Composition for preventing or treating parasitic infections in fish, comprising Sophora flavescens Aiton extract or fraction thereof |
| GB2583664B (en) * | 2018-01-29 | 2022-09-14 | Jeonjin Bio Co Ltd | Composition for preventing or treating sea lice infections in fish, comprising an extract of Sophora Flavescens Aiton or a fraction thereof |
| KR102155499B1 (en) * | 2019-09-06 | 2020-09-14 | 주식회사 가온아이피엠 | Insect Repellent Composition Using Sophora flavescens and Morinda officinalis and Method for Manufacturing the Same |
| CN110742096A (en) * | 2019-11-26 | 2020-02-04 | 余庆县玉笏春茶业有限责任公司 | Small leaf broadleaf holly leaf insect-resist agent and preparation method thereof |
| KR102212587B1 (en) * | 2020-07-14 | 2021-02-05 | 주식회사 가온아이피엠 | Orientalis McLachlan Repellent Composition Using Sophora flavescens and Morinda officinalis and Method for Manufacturing the Same |
| KR102212590B1 (en) * | 2020-07-17 | 2021-02-05 | 주식회사 가온아이피엠 | Natural pesticide composition harmless to human body and manufacturing method thereof |
| KR102361490B1 (en) * | 2021-08-18 | 2022-02-14 | 이용덕 | Eco-friendly pest insecticide composition for livestock and method for controlling pests by using the same |
| KR20240024438A (en) | 2022-08-17 | 2024-02-26 | 국립안동대학교 산학협력단 | Wax-type repellent composition against Thrips using alarm pheromone and control method of Thrips using it |
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