WO2019146826A1 - Composition for preventing or treating parasitic infections in fish, comprising sophora flavescens aiton extract or fraction thereof - Google Patents

Composition for preventing or treating parasitic infections in fish, comprising sophora flavescens aiton extract or fraction thereof Download PDF

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WO2019146826A1
WO2019146826A1 PCT/KR2018/001249 KR2018001249W WO2019146826A1 WO 2019146826 A1 WO2019146826 A1 WO 2019146826A1 KR 2018001249 W KR2018001249 W KR 2018001249W WO 2019146826 A1 WO2019146826 A1 WO 2019146826A1
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fish
extract
preventing
pharmaceutical composition
fraction
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PCT/KR2018/001249
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French (fr)
Korean (ko)
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이태훈
최원식
정태성
손세진
김태규
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전진바이오팜 주식회사
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Priority to PCT/KR2018/001249 priority Critical patent/WO2019146826A1/en
Priority to CA3088618A priority patent/CA3088618C/en
Priority to NO20181070A priority patent/NO345471B1/en
Priority to GB2010954.2A priority patent/GB2583664B/en
Publication of WO2019146826A1 publication Critical patent/WO2019146826A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/489—Sophora, e.g. necklacepod or mamani
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/10—Anthelmintics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the present invention relates to a composition for preventing or treating a parasitic infectious disease of fish comprising an extract of Sophora flavescens Aiton as an active ingredient, and more particularly to a composition for preventing or treating parasitic infectious disease of fish comprising an extract of Gossam gum, a fraction thereof or a compound isolated therefrom as an effective ingredient ,
  • salmon aquaculture is an alternative to depletion of fishery resources and is mainly focused on fish species with excellent commercial characteristics.
  • salmon contains many unsaturated fatty acids such as DHA and EPA, The world's largest fish species, the world's largest salmon aquaculture country is Norway, followed by England, Chile, Canada and Australia.
  • the common feature of salmon cultivation stations is that they are located in the waters where cold seawater flows.
  • the global salmon market is estimated at 4.3 million tons and 20 trillion won a year, including around 60 trillion won if it includes the processing market.
  • Cherry salmon is a salmon inhabited by rivers and oceans of Korea, China, Japan and Russia. Its size is 60 cm smaller than 1 meter of Norwegian salmon. We call it Shima salmon which is Japanese name. In other words, in Korea, salmon and trout form, but they do not use the name salmon but chuck salmon (trout). Trout fishing and festivals in winter are famous. In Korea, the development of freshwater-based inland water trout farming has developed, and in 2015 it was the 50th anniversary of trout farming. Meanwhile, salmon can be classified into Pacific salmon and Atlantic salmon. In Korea, silver salmon belonging to Pacific salmon is being tried. On the other hand, Atlantic salmon is being cultivated in large scale in North Sea fjord such as Norway, and it is cultivated in Chile and Canada in the Pacific coast. Korea is still under trial research.
  • Patent Document 1 KR1020010109335 A
  • the present invention provides a composition for prevention or treatment of infectious diseases caused by fish parasites including sea lice in an eco-friendly manner without causing tolerance, toxicity and environmental pollution by using gosam, a food material harmless to human body have.
  • the present invention provides a pharmaceutical composition for preventing or treating a fish parasitic infectious disease comprising Sophora flavescens Aiton extract or a fraction thereof as an active ingredient.
  • the extract of the present invention is a leaf, a stem, a root, a flower, a fruit or an outpast of a ginseng, and the extract includes matrine, oxymatrine, anagyrine, sophoranol, cytisine ), Methylcytisine, kurarinone, xanthohumol, Noranhydroicaritin, isoanhydroicaritin, glucoluteolin, chemperol-3- Kaempferol-3-sophoroside, Heilsteine, and Heilsteine-4-rhamnosyl glucoside.
  • the ginseng extract according to the present invention can be obtained by ultrasonic extraction, hot water extraction or solvent extraction, but is not limited thereto.
  • the fish parasite is selected from the group consisting of sea lice, Cymothoa Exigua, Ichthyophthirius multifiliis, Bivagina tai, Anisakiasis, Distoma, Scutica, Benedenia or Anisakis Simplex, preferably in the genus Lepeophtheirus or in the caligus, in water, caligidae, More preferred are sea lice, Lepeoptheirus salmonis or caligus rogercresseyi.
  • fish can be collectively referred to as any fish to which the parasitic infectious disease according to the present invention can develop without limitation, and preferably salmon, trout, catfish, perch, tuna, halibut, arctic fish, sturgeon, It includes a lot of fish, sandfish, tiger prawn, carp, tilapia, rower, eel, sea bream, yellow tail, amber jacquard, jambar, rockfish, red sea bream, red sea bream, flounder, mackerel, . It is also preferably, but not exclusively, aquaculture. It is preferably Salmonidae.
  • the present invention also provides a feed additive composition for preventing or improving a fish parasitic infection comprising an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredient.
  • the present invention also provides a composition for controlling or insecting a fish parasite, which contains Sophora flavescens Aiton extract or a fraction thereof as an active ingredient.
  • the present invention provides a method for preventing or treating parasitic infestation of fish by injecting a composition containing an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredient into fish.
  • composition comprising the extract of the present invention or the fraction thereof as an active ingredient can be used as a preventive or therapeutic agent for fish parasites because it prevents sea lice or prevents the access of parasites when consumed by fish .
  • Figure 1 shows a Y-tube chamber for sea lice avoidance experiments.
  • Fig. 2 shows the results of the seaweed avoidance effect of the ginseng extract.
  • Figs. 3 (a) and 3 (b) show the death, drowning and survival rate of sea lice in a concentration-dependent manner.
  • Gosam is a perennial plant belonging to the rosemary bean family of dicotyledonous plants. It is a wild flower native to the mountain areas of the country.
  • the scientific name of Gosam is Sophorae flavescens Aiton, and the name Gosam uses the letter 'Gosu' with the taste and the word 'Gosan' because it has similar efficacy. It is mainly used as a medicinal herb, and it has been used for the treatment of leukopenia, anti-radiation action, lowering of blood sugar, antitumor, antimicrobial activity, suppression of immune function, etc.
  • ginseng can be used as a biological organism or can be subjected to one or more processes through a drying process such as natural drying, oven drying, hot air drying, freeze drying, or the like, or by a method such as juicing, grinding,
  • extract in the present invention means an extract obtained by extracting the above-mentioned ginseng, a diluted solution or concentrate of the extract, a dried product obtained by drying the extract, a controlled preparation or a purified product of the extracted solution, And extracts of all formulations which can be formed using extracts.
  • the method for extracting ginseng is not particularly limited and may be carried out according to a method commonly used in the art.
  • Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.
  • the type of the extraction solvent used for extracting the ginseng is not particularly limited, and any solvent known in the art can be used.
  • Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof.
  • Water, a lower alcohol, 1,3-butyleneglycol, and ethyl acetate may be used alone or in admixture of two or more.
  • the extract may be extracted with a solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms and a mixed solvent thereof, and the alcohol may be methanol, ethanol, propyl alcohol or butanol, But is not limited to.
  • the extract is naturally dried for 5 to 7 days at the shade and at room temperature after harvesting, and pulverized to about 1 to 20 times, preferably about 1 to 5 times (C1) to 4 (C4) lower alcohols such as methanol, ethanol and butanol, or a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10 thereof, as an elution solvent Extraction is carried out at a temperature of 20 to 100 ° C, preferably at room temperature, for an extraction period of about 12 to 6 days, preferably 5 days, such as hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction .
  • C1 to 5 times C1 to 4 (C4) lower alcohols
  • C1 to 4 (C4) lower alcohols such as methanol, ethanol and butanol
  • a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10 thereof as an elution solvent Extraction is carried out at a temperature of 20 to 100 ° C, preferably at room temperature, for an extraction period of about 12 to 6 days
  • fraction in the present invention means a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.
  • the fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art.
  • a method in which a fraction obtained from the extract is treated with a predetermined solvent by an extract obtained by extracting the extract.
  • the compounds contained in the extract of Gossam gum extract include matrine, oxymatrine, anagyrine, sophoranol, cytisine, methylcytisine, xanthohumol, noranhydroicaritin, isoanhydroicaritin, glucoluteolin, kaempferol-3-sophoroside, and the like. Heilsteine, Heilsteine-4-rhamnosyl glucoside, etc. These compounds can be obtained from the extract or the fraction by a conventional separation and purification process.
  • the fish may be any fish which can cause parasitic infectious diseases according to the present invention without limitation, and preferably includes salmon, trout, catfish, perch, tuna, halibut, arctic fish, sturgeon, Includes prawns, staves, westerns, carp, tilapia, lobsters, eels, sea bream, yellow tail, amberjack, jambari, rockfish, red sea bream, red sea bream, flounder, mackerel, can do. It is also preferably, but not exclusively, aquaculture. In one embodiment of the present invention, an experiment was conducted using a representative salmon
  • the parasite of the present invention collectively refers to an invertebrate living in parasitic life outside and in the body of another organism (fish, crustacean, animal, plant, etc.).
  • the extract of the ginseng extract has an effect of exterminating the sea lice (FIGS. 2 and 3).
  • the present invention provides a method for preventing, improving or treating a parasite infection .
  • the term “prevent” means any action that inhibits or slows the onset of parasitic infectious disease of fish by the composition
  • treatment means that the composition of the fish, Means any action that is changed.
  • composition in the present invention means a preparation for the purpose of prevention or treatment of disease, and may be formulated into various forms according to ordinary methods.
  • oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions and syrups, and can be formulated in the form of external preparations, suppositories, and sterilized injection solutions.
  • the composition of the present invention is provided in the form of a bathing agent for the purpose of treating diseases of fishes.
  • each of the formulations may be further prepared by incorporating pharmaceutically acceptable carriers such as buffers, preserving agents, wetting agents, solubilizing agents, isotonic agents, stabilizers, bases, excipients, lubricants and the like in the art .
  • pharmaceutically acceptable carriers such as buffers, preserving agents, wetting agents, solubilizing agents, isotonic agents, stabilizers, bases, excipients, lubricants and the like in the art .
  • the pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount.
  • pharmaceutically effective amount of the present invention means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to veterinary treatment and not causing side effects, , The severity, the activity of the drug, the sensitivity to the drug, the method of administration, the time of administration, the route of administration and the rate of release, the duration of the treatment, the factors including the combined or concurrently used drugs, and other factors well known in the veterinary arts.
  • the present invention provides a feed additive composition for preventing or ameliorating a parasitic infestation disease of fish, comprising an extract of Gossam gum, a fraction thereof, or a compound isolated therefrom as an active ingredient.
  • the extract of Gossam gum, its fractions, or the compounds isolated therefrom can be added to the feed composition as a feed additive as it can be used for the purpose of preventing or improving the parasitic infestation of fish.
  • the feed additive is collectively referred to as a substance added to the feed in a nutritive or special manner for a specific purpose.
  • it means a substance added for the purpose of preventing or improving a parasitic infectious disease of a fish.
  • the ginseng extract of the present invention or the compound isolated therefrom may be included in the feed composition as the feed additive, and the feed may be used to collectively mean all the foods supplied to the fish.
  • the feed composition of the present invention may further contain a binder, an emulsifying agent, a preservative, and the like added to prevent quality deterioration.
  • the feed composition may contain an amino acid, a vitamin, an enzyme, a probiotics, a flavoring agent, A compound, a silicate, a buffer, a coloring agent, an extracting agent, an oligosaccharide, and the like, and may further include, but is not limited to, a feed mixture.
  • the compound feed commonly used in the feed composition for fish includes any one or more protein sources selected from fish meal, casein, bone meal, and shrimp powder, or any one or more carbohydrate sources selected from sweet potato starch, potato starch, wheat starch, But the present invention is not limited thereto. Even if it is used in combination with the compound feed, it is possible to prevent or ameliorate parasitic infectious diseases according to the present invention, The effect is the same.
  • Another aspect of the present invention provides a method for preventing or treating parasitic infestation in fish, comprising the step of administering a composition containing an extract of rosemary extract, fraction thereof, or a compound isolated therefrom as an active ingredient, directly to a fish or to a fish farm. do.
  • Another aspect of the present invention provides a method for preventing or treating parasitic infestation in a fish, comprising the step of administering a pharmaceutical composition or feed additive composition containing the extract as an active ingredient directly to a fish or in a farm.
  • the method for preventing or treating a parasitic infectious disease of a fish may include any step in which the composition of the present invention is directly or indirectly delivered to a fish by including the step of administering the fish directly or in a farm.
  • the purpose of the present invention may be accomplished by directly injecting or feeding fish, and may also be accomplished by means of bathing or eating.
  • the effective concentration of the composition may be coated on the feed and fed to fish having lesions due to parasitic infectious disease, but is not limited thereto.
  • the dose of the composition may vary depending on the weight of the fish to be treated, the health condition, the administration time, and the severity of the disease.
  • the roots were thoroughly washed with water, naturally dried for 5 to 7 days, and then cut. Three times as much methanol as weight was added to 50 kg of the cut gosam, and the mixture was extracted at room temperature for 5 days and then filtered. It was concentrated under reduced pressure, lyophilized and powdered.
  • the control section is flushed with clean sea water and the compound section is flushed with the substance to be tested.
  • Mixed section is a section where control and compound sections are mixed and drained.
  • the sea lice are located at the point where the mixed section starts and the movement is observed.
  • the interpretation of the experimental results is carried out in the opposite direction in which the test substance flows, that is, the control section and the mixed section section), the sea lice effect was confirmed by analyzing how long the sea lice moved and stayed. (See Fig. 1)
  • the experiment was carried out using a 24-well plate to confirm the effect of the sea lice on the sea lice. All the solutions used in the experiment were kept at 10 ° C during the experiment. After incubation for 24 hours, sea lice were added to each well. The effect of sea lice eradication was confirmed by observing the condition.
  • the ginseng extract or its fractions of the present invention have anti-sea lice activity and thus can be used for the prevention or treatment of parasitic infectious diseases of fish, the prevention of parasites, and the control thereof.
  • the present invention is recognized as an industrial applicability in the fields of pharmaceutical biotechnology and agriculture and fisheries.

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Abstract

The present invention relates to a composition for preventing or treating parasitic infectious diseases in fish, comprising a Sophora flavescens Aiton (Jerusalem artichoke) extract or a fraction thereof as an active ingredient, and specifically to a pharmaceutical composition for preventing or treating diseases caused by infection with sea lice, which are fish parasites, a feed additive composition for preventing or alleviating said diseases, and a method for preventing or treating parasitic infectious diseases in fish, comprising the step of introducing said compositions into fish directly or to a fish farm.

Description

고삼 추출물 또는 이의 분획물을 포함하는 어류 기생충 감염의 예방 또는 치료용 조성물Composition for preventing or treating fish parasitic infestation, comprising the extract or its fraction
본 발명은 고삼(Sophora flavescens Aiton) 추출물을 유효성분으로 포함하는 어류의 기생충 감염 질환 예방 또는 치료용 조성물에 관한 것으로서, 보다 구체적으로 고삼추출물, 이의 분획물 또는 이로부터 분리된 화합물을 유효성분으로 포함하는, 어류의 기생충 감염 질환 예방 또는 치료용 약학 조성물, 예방 또는 개선용 사료첨가제 조성물, 및 상기 조성물을 어류에 직접 또는 양식장에 투입하는 단계를 포함하는 어류의 기생충 감염 질환 예방 또는 치료방법에 관한 것이다. The present invention relates to a composition for preventing or treating a parasitic infectious disease of fish comprising an extract of Sophora flavescens Aiton as an active ingredient, and more particularly to a composition for preventing or treating parasitic infectious disease of fish comprising an extract of Gossam gum, a fraction thereof or a compound isolated therefrom as an effective ingredient , A pharmaceutical composition for preventing or treating a parasitic infectious disease of fish, a feed additive composition for prevention or improvement, and a method for preventing or treating a parasitic infectious disease in a fish, comprising the step of putting the composition directly into a fish or in a farm.
세계적으로 어류 양식업은 수산자원의 고갈에 대한 대안으로 식품으로 선호하고 상품성이 우수한 어종을 중심으로 이루어지고 있으며, 그 중 연어는 DHA, EPA 등 우리 몸에 이로운 불포화 지방산이 많이 포함되어 있고 맛도 뛰어나 전 세계적으로 선호하는 어종으로서 세계최대 연어 양식국은 노르웨이이며, 뒤이어 영국, 칠레, 캐나다, 호주 등이 양식을 하고 있다. 연어 양식국의 공통점은 차가운 해수가 흐르는 해역에 위치한다는 점이다. 전세계 연어시장은 연간 약 4.3백만톤, 20조원 규모이며, 가공시장을 포함할 경우 약 60조원에 이른다. Worldwide, fish aquaculture is an alternative to depletion of fishery resources and is mainly focused on fish species with excellent commercial characteristics. Among them, salmon contains many unsaturated fatty acids such as DHA and EPA, The world's largest fish species, the world's largest salmon aquaculture country is Norway, followed by Britain, Chile, Canada and Australia. The common feature of salmon cultivation stations is that they are located in the waters where cold seawater flows. The global salmon market is estimated at 4.3 million tons and 20 trillion won a year, including around 60 trillion won if it includes the processing market.
한편 한국에서는 연어과인 송어를 주로 양식하고 있으며, 송어(Cherry salmon)는 한국, 중국, 일본, 러시아의 강과 바다에 서식하는 연어과로서, 크기는 노르웨이 연어의 1미터 보다 작은 60 cm이다. 일본 명칭인 시마연어로도 부른다. 즉, 한국에도 연어과인 송어가 서식하나 연어라는 명칭을 사용하지 않고 송어(cherry salmon, trout)라고 부르는 것이다. 겨울철 송어낚시와 축제가 유명하다. 우리나라는 담수를 기반으로 한 내수면 송어양식이 발전을 이루었고, 2015년은 송어양식 50주년이기도 하였다. 한편, 연어는 태평양 연어와 대서양 연어로 구분할 수 있는데, 우리나라는 태평양연어에 속하는 은연어 양식이 시도되고 있다. 한편, 대서양 연어는 노르웨이 등 북해 피오르드에서 대규모 양식되고 있으며, 태평양 연안에서는 칠레, 캐나다 등에서 양식하고 있다. 우리나라는 아직 시험 연구 중에 있다.Cherry salmon is a salmon inhabited by rivers and oceans of Korea, China, Japan and Russia. Its size is 60 cm smaller than 1 meter of Norwegian salmon. We call it Shima salmon which is Japanese name. In other words, in Korea, salmon and trout form, but they do not use the name salmon but chuck salmon (trout). Trout fishing and festivals in winter are famous. In Korea, the development of freshwater-based inland water trout farming has developed, and in 2015 it was the 50th anniversary of trout farming. Meanwhile, salmon can be classified into Pacific salmon and Atlantic salmon. In Korea, silver salmon belonging to Pacific salmon is being tried. On the other hand, Atlantic salmon is being cultivated in large scale in North Sea fjord such as Norway, and it is cultivated in Chile and Canada in the Pacific coast. Korea is still under trial research.
이러한 양식 중 연어양식을 가장 알려져있는 노르웨이의 마린 하베스트사(Marine Harvest)는 연어양식을 1960년대에 성공했으며, 지역 어민이 차린 소기업이 오늘날 세계적 기업으로 성장했다. 현재 세계최대의 연어 양식 회사로서 연간 1억개의 연어알을 부화시키며 약 3조원 가량을 연어 양식으로 번다.Marine Harvest, Norway's most known form of salmon, has succeeded in salmon farming in the 1960s, and small businesses with local fishermen have grown into a global company today. Currently, the largest salmon farming company in the world, it hatches 100 million salmon eggs a year and earns about 3 trillion won in salmon style.
그러나, 현재 양식업의 가장 문제점은 폐사율이며 연간 20%를 상회하고 있고, 그 주요원인으로 수온 상승으로 인한 난치성 질병 발생 및 감염 (바이러스, 세균, 기생충), 약제 치료효과의 저감이 지목되고 있다. 특히 사육 밀집도가 높은 양식장에는 "바다이(sea lice)"가 필수적으로 발생하게 되는데, 바다이가 붙은 연어는 물리적, 화학적 손상에 의해 여러 가지 부작용을 낳는다. '바다이'는 노르웨이뿐 아니라 스코틀랜드, 캐나다 등지에서 양식되고 있는 대서양 연어에 많이 기생하는 외부 기생충이다. 바다 이가 한번 발생하면 폭발적으로 전염될 수 있다. 상세하게는 어류에 대한 바다 이(Sea lice)는 외부기생충으로 숙주인 어류의 점액, 표피조직 또는 혈액을 먹고 산다. 바다이(Sea lice)에 존재하는 단백질 성분이 어류의 체외조직에 붙어 숙주로 삼아 기생생활을 하게 되는 것이다.바다이(Sea lice)가 어류에 기생하게 되면 염증, 병원균 등을 증폭시키며, 폐사율이 급증되고 어류의 개체수가 감소되는 심각한 문제를 가지고 있다. 왜냐하면 가두리 양식장 내에 어류가 집약적으로 많이 모여서 양식되고 있기 때문에 노르웨이나 스코틀랜드에서는 30~40%정도 까지 연어가 폐사되는 경우도 있다고 한다.However, the most problematic of the aquaculture industry is the mortality rate, which is more than 20% per year. The main cause of this is the incidence of intractable diseases and infection (viral, bacterial, parasitic) Especially in a farm where breeding density is high, "sea lice" is essential. Salmon with seaweed causes various side effects by physical and chemical damage. 'SeaIye' is a parasitic larva that is parasitic to Atlantic salmon cultured in Norway, Scotland and Canada. Once a sea bug occurs, it can spread explosively. Specifically, sea lice for fish are external parasites that live on the mucus, epidermal tissue or blood of the host fish. Sea lice are parasitic on the fish, which causes the fish to multiply, inflammation, pathogens, etc., and the mortality rate increases rapidly. There is a serious problem that the population of fish is reduced. In Norway and Scotland, there are cases where salmon are killed by 30 to 40% because fish are concentrated and cultivated intensively in the cage farm.
과거에는 양식업자들이 연어 양식을 위해 사료에 살충제를 넣어서 이 문제를 해결하고자 하였으나, 최근에는 이 화학물질에 내성을 가진 바다이가 출현하였다. 현재 마린 하베트스는 어장에 feeder fish라는 자연적으로 연어의 바다이(sea lice)를 먹어치우는 어류를 도입하여 바다 이의 수를 줄이기 위해 노력하고 있다. 전문가들은 살충제와 같은 오래된 관리수단과 유전적 내성 배양법과 같은 신규 전략을 포함한 융합기술로 바다이 퇴치가 가능할 것이라고 예측하고 있으나 아직 그 성과가 성공적이지 않으며, 현재까지‘에마멕틴벤조에이트(emamectin benzoate)’를 함유한 살충제 사용이 가장 효율적인 수단에 불과한 실정이다. In the past, aquaculturists tried to solve this problem by putting insecticides on feed for salmon farming, but recently, marine species resistant to this chemical have appeared. Currently, Marine Habitus is trying to reduce the number of seas by introducing fish that feed on the sea lice naturally called feeder fish. Experts predict that marine extermination will be possible with fusion technologies including new management strategies such as insecticides and new management strategies such as genetic resistance culture, but the results have not been successful yet, and the 'emamectin benzoate' The use of insecticides containing only insecticides is the most effective means.
(특허문헌 1) KR1020010109335 A (Patent Document 1) KR1020010109335 A
본 발명은 인체에 무해한 식품소재인 고삼을 이용하여 내성, 독성 및 환경오염을 유발시키지 않고, 친환경적으로 바다 이(Sea lice)를 포함하는 어류 기생충에 의한 감염 질환의 예방 또는 치료용 조성물을 제공하는데 있다.The present invention provides a composition for prevention or treatment of infectious diseases caused by fish parasites including sea lice in an eco-friendly manner without causing tolerance, toxicity and environmental pollution by using gosam, a food material harmless to human body have.
또한 본 발명은 고삼 추출물을 유효성분으로 하는 사료첨가제 조성물을 이용하여 어류 기생충의 방제, 살충 및 퇴치하는데 그 목적이 있다.It is another object of the present invention to control, insect and eradicate fish parasites by using a feed additive composition containing an extract of Gossam gypsum as an active ingredient.
본 발명은 고삼(Sophora flavescens Aiton) 추출물 또는 이의 분획물을 유효성분으로 포함하는 어류 기생충 감염 질환의 예방 또는 치료용 약학 조성물을 제공한다. 상기 고삼 추출물은 고삼의 잎, 줄기, 뿌리, 꽃, 열매 또는 전초이며, 추출물에는 마트린(matrine), 옥시마트린(oxymatrine), 아나기린(anagyrine), 소포라놀(sophoranol), 시티진(cytisine), 메틸시티진(methylcytisine), 쿠라리논(kurarinone), 잔토휴몰(xanthohumol), 노르안하이드로이카리딘(Noranhydroicaritin), 이소안하이드로이카리딘(isoanhydroicaritin), 글루코루데올린(glucoluteolin), 켐페롤-3-소포로시드(kaempferol-3-sophoroside), 헤니스테인(Heilsteine), 헤니스테인-4-람노실글리코시드(Heilsteine-4-rhamnosyl glucoside)이 포함되어 있다.The present invention provides a pharmaceutical composition for preventing or treating a fish parasitic infectious disease comprising Sophora flavescens Aiton extract or a fraction thereof as an active ingredient. The extract of the present invention is a leaf, a stem, a root, a flower, a fruit or an outpast of a ginseng, and the extract includes matrine, oxymatrine, anagyrine, sophoranol, cytisine ), Methylcytisine, kurarinone, xanthohumol, Noranhydroicaritin, isoanhydroicaritin, glucoluteolin, chemperol-3- Kaempferol-3-sophoroside, Heilsteine, and Heilsteine-4-rhamnosyl glucoside.
본 발명에 따른 상기 고삼 추출물은 초음파추출, 열수추출 또는 용매추출에 의해 얻어질 수 있으며, 이에 한정되지는 않는다.  The ginseng extract according to the present invention can be obtained by ultrasonic extraction, hot water extraction or solvent extraction, but is not limited thereto.
본 발명에서 상기 어류 기생충은 바다 이(sea lice), 시모토아 에시구아(Cymothoa Exigua), 백점충(Ichthyophthirius multifiliis), 비바기나충(Bivagina tai), 아나사키스(Anisakiasis), 디스토마(Distoma), 촌충(Tapeworm), 스쿠치카(Scutica), 베네데니아(Benedenia) 또는 고래회충(Anisakis Simplex)이며, 바람직하게는 물이과(caligidae)에서, 레페오프테이루스(Lepeophtheirus) 속 또는 칼리구스(caligus) 속이고, 보다 바람직하게는 바다이(sea lice), 레페오프테이루스 살모니스(Lepeoptheirus salmonis) 또는 칼리구스 로게르세르세이지 (caligus rogercresseyi)이다.In the present invention, the fish parasite is selected from the group consisting of sea lice, Cymothoa Exigua, Ichthyophthirius multifiliis, Bivagina tai, Anisakiasis, Distoma, Scutica, Benedenia or Anisakis Simplex, preferably in the genus Lepeophtheirus or in the caligus, in water, caligidae, More preferred are sea lice, Lepeoptheirus salmonis or caligus rogercresseyi.
본 발명에서 어류는 본 발명에 따른 기생충 감염 질환이 발병할 수 있는 모든 어류를 제한없이 통칭할 수 있으며, 바람직하게는 연어, 송어, 메기, 농어, 참치, 넙치, 북극 곤들매기, 철갑상어, 평어, 도다리, 서대, 잉어, 틸라피아, 줄농어, 뱀장어, 바다브림, 엘로우테일, 앰버잭, 참바리, 조피볼락, 참돔, 돌돔, 가자미, 고등어, 능성어, 참다랑어, 감성동, 방어, 부시리 등을 포함할 수 있다. 또한, 바람직하게는 양식 어류일 수 있으나 이에 제한되지 않는다. 바람직하게는 연어과 어류(Salmonidae)이다.In the present invention, fish can be collectively referred to as any fish to which the parasitic infectious disease according to the present invention can develop without limitation, and preferably salmon, trout, catfish, perch, tuna, halibut, arctic fish, sturgeon, It includes a lot of fish, sandfish, tiger prawn, carp, tilapia, rower, eel, sea bream, yellow tail, amber jacquard, jambar, rockfish, red sea bream, red sea bream, flounder, mackerel, . It is also preferably, but not exclusively, aquaculture. It is preferably Salmonidae.
또한 본 발명은 고삼(Sophora flavescens Aiton) 추출물 또는 이의 분획물을 유효성분으로 포함하는 어류 기생충 감염 예방 또는 개선용 사료첨가제 조성물을 제공한다.The present invention also provides a feed additive composition for preventing or improving a fish parasitic infection comprising an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredient.
또한 본 발명은 고삼(Sophora flavescens Aiton) 추출물 또는 이의 분획물을 유효성분으로 포함하는 어류 기생충 방제 또는 살충용 조성물을 제공한다.The present invention also provides a composition for controlling or insecting a fish parasite, which contains Sophora flavescens Aiton extract or a fraction thereof as an active ingredient.
또 다른 예로, 본 발명은 고삼(Sophora flavescens Aiton) 추출물 또는 이의 분획물을 유효성분으로 포함하는 조성물을 어류에 투입하여 어류의 기생충 감염을 예방 또는 치료하는 방법을 제공한다.As another example, the present invention provides a method for preventing or treating parasitic infestation of fish by injecting a composition containing an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredient into fish.
본 발명에서 제공하는 고삼 추출물 또는 이의 분획물을 유효성분으로 포함하는 조성물은 바다이(sea lice)가 기피하거나 어류가 섭취시 기생충의 접근을 억제 또는 퇴치하게 유도하므로 어류의 기생충 예방 또는 치료제로 사용할 수 있다. The composition comprising the extract of the present invention or the fraction thereof as an active ingredient can be used as a preventive or therapeutic agent for fish parasites because it prevents sea lice or prevents the access of parasites when consumed by fish .
도 1은 바다이(sea lice) 기피 실험을 위한 와이튜브 챔버(Y-tube chamber)를 나타낸다.Figure 1 shows a Y-tube chamber for sea lice avoidance experiments.
도 2는 고삼 추출물의 바다이 기피효과에 대한 결과를 나타낸다.Fig. 2 shows the results of the seaweed avoidance effect of the ginseng extract.
도 3(a) 및 (b)은 농도의존적으로 바다이(sea lice)의 사멸, 빈사, 생존수를 나타낸다.Figs. 3 (a) and 3 (b) show the death, drowning and survival rate of sea lice in a concentration-dependent manner.
고삼은 쌍떡잎식물 장미목 콩과에 속하는 여러해살이 풀이며 전국의 산지에 자생하는 야생화로 꽃은 층상꽃차례로 핀다. 고삼의 학명은 Sophorae flavescens Aiton이며, 고삼이라는 이름은 맛이 써서 '고'라는 글자를 사용하고 효능이 삼과 유사하다하여 '삼'이라는 글자를 사용한다. 주로 뿌리를 약재로 사용하며 거풍살충, 청열이노, 청열조습 작용이 있다고 하여 백혈구감소증 치료 및 항방사능 작용, 혈당을 낮추는 작용, 항종양, 항균, 면역기능 억제 등에 사용되고 있다.Gosam is a perennial plant belonging to the rosemary bean family of dicotyledonous plants. It is a wild flower native to the mountain areas of the country. The scientific name of Gosam is Sophorae flavescens Aiton, and the name Gosam uses the letter 'Gosu' with the taste and the word 'Gosan' because it has similar efficacy. It is mainly used as a medicinal herb, and it has been used for the treatment of leukopenia, anti-radiation action, lowering of blood sugar, antitumor, antimicrobial activity, suppression of immune function, etc.
본 발명에서 고삼은 생물로 사용하거나 자연건조, 오븐건조, 열풍건조, 동결건조 등의 건조법을 이용한 건조물 또는 착즙, 분쇄, 절단, 파쇄 등의 방법을 통하여 한 가지 이상의 과정을 거친 고삼을 사용할 수 있다 In the present invention, ginseng can be used as a biological organism or can be subjected to one or more processes through a drying process such as natural drying, oven drying, hot air drying, freeze drying, or the like, or by a method such as juicing, grinding,
본 발명에서 용어 "추출물"은 상기 고삼의 추출처리에 의하여 얻어지는 추출액, 상기 추출액의 희석액이나 농축액, 상기 추출액을 건조하여 얻어지는 건조물, 상기 추출액의 조정제물이나 정제물, 또는 이들의 혼합물 등, 추출액 자체 및 추출액을 이용하여 형성 가능한 모든 제형의 추출물을 포함한다.The term "extract" in the present invention means an extract obtained by extracting the above-mentioned ginseng, a diluted solution or concentrate of the extract, a dried product obtained by drying the extract, a controlled preparation or a purified product of the extracted solution, And extracts of all formulations which can be formed using extracts.
본 발명의 상기 고삼 추출물에 있어서, 상기 고삼을 추출하는 방법은 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용하는 방법에 따라 추출할 수 있다. 상기 추출 방법의 비제한적인 예로는, 열수 추출법, 초음파 추출법, 여과법, 환류 추출법 등을 들 수 있으며, 이들은 단독으로 수행되거나 2 종 이상의 방법을 병용하여 수행될 수 있다.In the ginseng extract of the present invention, the method for extracting ginseng is not particularly limited and may be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.
본 발명에서 상기 고삼을 추출하는 데에 사용되는 추출 용매의 종류는 특별히 제한되지 아니하며, 당해 기술 분야에서 공지된 임의의 용매를 사용할 수 있다. 상기 추출 용매의 비제한적인 예로는 물; 메탄올, 에탄올, 프로필알코올, 부틸알코올 등의 C1내지 C4의 저급 알코올; 글리세린, 부틸렌글라이콜, 프로필렌글라이콜 등의 다가알코올; 및 메틸아세테이트, 에틸아세테이트, 아세톤, 벤젠, 헥산, 디에틸에테르, 디클로로메탄 등의 탄화수소계 용매; 또는 이들의 혼합물을 사용할 수 있으며, 바람직하게, 물, 저급알코올, 1,3-부틸렌글라이콜, 에틸아세테이트를 단독으로 사용하거나 2종 이상 혼합하여 사용할 수 있다. 본 발명에서 바람직하게, 상기 추출물은 물, 탄소수 1 내지 4의 알코올 및 이들의 혼합 용매로 구성된 군에서 선택되는 용매로 추출한 것일 수 있으며, 상기 알코올은 메탄올, 에탄올, 프로필알코올 또는 부탄올일 수 있으나 이에 제한되는 것은 아니다.In the present invention, the type of the extraction solvent used for extracting the ginseng is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof. Water, a lower alcohol, 1,3-butyleneglycol, and ethyl acetate may be used alone or in admixture of two or more. In the present invention, the extract may be extracted with a solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms and a mixed solvent thereof, and the alcohol may be methanol, ethanol, propyl alcohol or butanol, But is not limited to.
본 발명에서 고삼 추출물을 얻기 위한 방법으로 보다 바람직하게는 고삼을 수확 후 음지 및 실온에서 5 내지 7일 동안 자연건조시키고, 분쇄하여 건조 중량의 약 1 내지 20배, 바람직하게는 약 1 내지 5배에 달하는 부피의 물, 메탄올, 에탄올 및 부탄올 등과 같은 탄소수 1(C1)내지 4(C4)의 저급 알콜의 극성 용매 또는 이들의 약 1:0.1 내지 1:10의 혼합비를 갖는 혼합용매를 용출 용매로써 사용하고, 추출 온도는 20 내지 100℃, 바람직하게는 실온에서, 추출 기간은 약 12시간 내지 6일, 바람직하게는 5일 동안 열수 추출, 냉침 추출, 환류 냉각 추출 또는 초음파 추출 등의 추출방법을 사용하여 추출할 수 있다. As a method for obtaining the extract of the extract of the present invention, it is more preferred that the extract is naturally dried for 5 to 7 days at the shade and at room temperature after harvesting, and pulverized to about 1 to 20 times, preferably about 1 to 5 times (C1) to 4 (C4) lower alcohols such as methanol, ethanol and butanol, or a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10 thereof, as an elution solvent Extraction is carried out at a temperature of 20 to 100 ° C, preferably at room temperature, for an extraction period of about 12 to 6 days, preferably 5 days, such as hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction .
본 발명에서 용어 "분획물"은 여러 다양한 구성 성분들을 포함하는 혼합물로부터 특정 성분 또는 특정 성분 그룹을 분리하기 위하여 분획을 수행하여 얻어진 결과물을 의미한다.The term "fraction " in the present invention means a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.
본 발명에서 상기 분획물을 얻는 분획 방법은 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용하는 방법에 따라 수행될 수 있다. 상기 분획 방법의 비제한적인 예로는, 고삼 추출하여 얻은 추출물에 소정의 용매를 처리하여 상기 추출물로부터 분획물을 얻는 방법을 들 수 있다.The fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art. As a non-limiting example of the above-mentioned fractionation method, there can be mentioned a method in which a fraction obtained from the extract is treated with a predetermined solvent by an extract obtained by extracting the extract.
본 발명에서 고삼 추출물에 포함된 화합물은 마트린(matrine), 옥시마트린(oxymatrine), 아나기린(anagyrine), 소포라놀(sophoranol), 시티진(cytisine), 메틸시티진(methylcytisine), 쿠라리논(kurarinone), 잔토휴몰(xanthohumol), 노르안하이드로이카리딘(Noranhydroicaritin), 이소안하이드로이카리딘(isoanhydroicaritin), 글루코루데올린(glucoluteolin), 켐페롤-3-소포로시드(kaempferol-3-sophoroside), 헤니스테인(Heilsteine), 헤니스테인-4-람노실글리코시드(Heilsteine-4-rhamnosyl glucoside) 등이 있으며, 이러한 화합물은 추출물 또는 분획물로부터 통상의 분리 정제과정을 거쳐 얻어질 수 있다.In the present invention, the compounds contained in the extract of Gossam gum extract include matrine, oxymatrine, anagyrine, sophoranol, cytisine, methylcytisine, xanthohumol, noranhydroicaritin, isoanhydroicaritin, glucoluteolin, kaempferol-3-sophoroside, and the like. Heilsteine, Heilsteine-4-rhamnosyl glucoside, etc. These compounds can be obtained from the extract or the fraction by a conventional separation and purification process.
본 발명에서 상기 어류는 본 발명에 따른 기생충 감염 질환이 발병할 수 있는 모든 어류를 제한없이 통칭할 수 있으며, 바람직하게는 연어, 송어, 메기, 농어, 참치, 넙치, 북극 곤들매기, 철갑상어, 평어, 도다리, 서대, 잉어, 틸라피아, 줄농어, 뱀장어, 바다브림, 엘로우테일, 앰버잭, 참바리, 조피볼락, 참돔, 돌돔, 가자미, 고등어, 능성어, 참다랑어, 감성동, 방어, 부시리 등을 포함할 수 있다. 또한, 바람직하게는 양식 어류일 수 있으나 이에 제한되지 않는다. 본 발명의 일 실시예에서는 대표적으로 연어를 사용하여 실험을 수행하였다In the present invention, the fish may be any fish which can cause parasitic infectious diseases according to the present invention without limitation, and preferably includes salmon, trout, catfish, perch, tuna, halibut, arctic fish, sturgeon, Includes prawns, staves, westerns, carp, tilapia, lobsters, eels, sea bream, yellow tail, amberjack, jambari, rockfish, red sea bream, red sea bream, flounder, mackerel, can do. It is also preferably, but not exclusively, aquaculture. In one embodiment of the present invention, an experiment was conducted using a representative salmon
본 발명의 기생충(Parasite)은 다른 생물체(어류, 갑각류, 동물, 식물 등)의 외부 및 몸속에서 기생생활을 하면서 사는 무척추동물을 총칭한다. 상세하게는 바다 이(sea lice), 시모토아 에시구아(Cymothoa Exigua), 백점충(Ichthyophthirius multifiliis), 비바기나충(Bivagina tai), 아나사키스(Anisakiasis), 디스토마(Distoma), 촌충(Tapeworm), 스쿠치카(Scutica), 베네데니아(Benedenia), 고래회충(Anisakis Simplex) 등이 있으며 이에 국한 되는 것은 아니다. 또한 내부기생(원생동물, 환형동물, 선형동물 등) 및 외부기생(편형동물, 절지동물 등) 어느 하나 이상을 포함한다. The parasite of the present invention collectively refers to an invertebrate living in parasitic life outside and in the body of another organism (fish, crustacean, animal, plant, etc.). In detail, the sea lice, Cymothoa Exigua, Ichthyophthirius multifiliis, Bivagina tai, Anisakiasis, Distoma, Tapeworm, Scutica, Benedenia, Anisakis Simplex, and the like. It also includes at least one of internal parasites (protozoa, annular animals, linear animals, etc.) and external parasites (flat animals, arthropods, etc.).
본 발명의 일 실시예에서는 상기 고삼 추출물이 바다이(sea lice)에 대하여 퇴치 효과가 있음을 확인하였으므로(도 2 및 도 3), 본 발명의 고삼 추출물을 포함한 어류의 기생충 감염 예방, 개선 또는 치료 용도에 적합하다는 것을 밝혔다.In one embodiment of the present invention, it has been confirmed that the extract of the ginseng extract has an effect of exterminating the sea lice (FIGS. 2 and 3). Thus, the present invention provides a method for preventing, improving or treating a parasite infection .
본 발명에서 용어 "예방"은 상기 조성물에 의해 어류의 기생충 감염 질환을 억제하거나 발병을 지연시키는 모든 행위를 의미하며, "치료"는 상기 조성물에 의해 어류의 기생충 감염 질환에 의한 증세가 호전되거나 이롭게 변경되는 모든 행위를 의미한다.In the present invention, the term "prevent" means any action that inhibits or slows the onset of parasitic infectious disease of fish by the composition, and "treatment" means that the composition of the fish, Means any action that is changed.
본 발명에서 용어 "약학 조성물"은 질병의 예방 또는 치료를 목적으로 제조된 것을 의미하며, 각각 통상의 방법에 따라 다양한 형태로 제형화하여 사용될 수 있다. 예컨대, 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽 등의 경구형 제형으로 제형화할 수 있고, 외용제, 좌제 및 멸균 주사용액의 형태로 제형화하여 사용될 수 있다. 다만, 본 발명의 조성물은 어류의 질병 치료라는 목적상 약욕제의 형태로 제공되는 것이 가장 바람직할 수 있다.The term "pharmaceutical composition" in the present invention means a preparation for the purpose of prevention or treatment of disease, and may be formulated into various forms according to ordinary methods. For example, it can be formulated into oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions and syrups, and can be formulated in the form of external preparations, suppositories, and sterilized injection solutions. However, it is most preferable that the composition of the present invention is provided in the form of a bathing agent for the purpose of treating diseases of fishes.
또한, 각각의 제형에 따라 약학적으로 허용가능한 담체, 예컨대 완충제, 보존제, 무통화제, 가용화제, 등장제, 안정화제, 기제, 부형제, 윤활제 등 당업계에 공지된 담체를 추가로 포함하여 제조할 수 있다.In addition, each of the formulations may be further prepared by incorporating pharmaceutically acceptable carriers such as buffers, preserving agents, wetting agents, solubilizing agents, isotonic agents, stabilizers, bases, excipients, lubricants and the like in the art .
한편, 본 발명의 약학조성물은 약학적으로 유효한 양으로 투여한다. 본 발명의 용어 "약학적으로 유효한 양"은 수의학적 치료에 적용 가능한 합리적인 수혜/위험 비율로 질환을 치료하기에 충분하며 부작용을 일으키지 않을 정도의 양을 의미하며, 유효용량 수준은 어류의 건강상태, 중증도, 약물의 활성, 약물에 대한 민감도, 투여 방법, 투여 시간, 투여 경로 및 배출 비율, 치료기간, 배합 또는 동시 사용되는 약물을 포함한 요소 및 기타 수의학 분야에 잘 알려진 요소에 따라 결정될 수 있다.Meanwhile, the pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. The term "pharmaceutically effective amount " of the present invention means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to veterinary treatment and not causing side effects, , The severity, the activity of the drug, the sensitivity to the drug, the method of administration, the time of administration, the route of administration and the rate of release, the duration of the treatment, the factors including the combined or concurrently used drugs, and other factors well known in the veterinary arts.
또 다른 하나의 양태로서, 본 발명은 고삼 추출물, 이의 분획물 또는 이로부터 분리된 화합물을 유효성분으로 포함하는, 어류의 기생충 감염 질환 예방 또는 개선용 사료첨가제 조성물을 제공한다.In another aspect, the present invention provides a feed additive composition for preventing or ameliorating a parasitic infestation disease of fish, comprising an extract of Gossam gum, a fraction thereof, or a compound isolated therefrom as an active ingredient.
본 발명의 목적상, 고삼 추출물, 이의 분획물, 또는 이로부터 분리된 화합물은 어류의 기생 감염 질환의 예방 또는 개선을 위한 목적으로 사용될 수 있기 때문에, 사료 첨가제로서 포함되어 사료 조성물에 첨가될 수 있다.For the purpose of the present invention, the extract of Gossam gum, its fractions, or the compounds isolated therefrom can be added to the feed composition as a feed additive as it can be used for the purpose of preventing or improving the parasitic infestation of fish.
본 발명에서 상기 사료 첨가제는 영양적 또는 특정 목적을 위하여 사료에 미량으로 첨가되는 물질을 총칭하는 것으로, 본 발명에서는 어류의 기생충 감염 질환 예방 또는 개선을 목적으로 첨가되는 물질을 의미한다. 본 발명의 고삼 추출물 또는 이로부터 분리된 화합물은 상기 사료 첨가제로서 사료 조성물에 포함될 수 있으며, 상기 사료는 어류에 공급되는 모든 식품을 총칭하는 의미로 사용될 수 있다.In the present invention, the feed additive is collectively referred to as a substance added to the feed in a nutritive or special manner for a specific purpose. In the present invention, it means a substance added for the purpose of preventing or improving a parasitic infectious disease of a fish. The ginseng extract of the present invention or the compound isolated therefrom may be included in the feed composition as the feed additive, and the feed may be used to collectively mean all the foods supplied to the fish.
본 발명의 사료 조성물에는 품질 저하를 방지하기 위해 첨가되는 결착제, 유화제, 보존제 등을 추가로 포함할 수 있고, 효용 증대를 위하여 첨가되는 아미노산제, 비타민제, 효소제, 생균제, 향미제, 비단백태 질소화합물, 규산염제, 완충제, 착색제, 추출제, 올리고당 등을 추가로 포함할 수 있으며, 그 외에도 사료 혼합제 등을 추가로 포함할 수 있으며, 이에 한정된 것은 아니다.The feed composition of the present invention may further contain a binder, an emulsifying agent, a preservative, and the like added to prevent quality deterioration. The feed composition may contain an amino acid, a vitamin, an enzyme, a probiotics, a flavoring agent, A compound, a silicate, a buffer, a coloring agent, an extracting agent, an oligosaccharide, and the like, and may further include, but is not limited to, a feed mixture.
또한, 상기 어류용 사료 조성물에 통상적으로 사용되는 배합사료로는 어분, 카제인, 육골분, 새우분말 중에서 선택되는 어느 하나 이상의 단백질원 또는 고구마 전분, 감자전분, 밀 전분 중에서 선택되는 어느 하나 이상의 탄수화물원 또는 채종유, 대두유, 옥수수유, 명태간유, 오징어간유 중에서 선택되는 어느 하나 이상의 지질원 등이 있으나 본 발명은 이에 한정되는 것은 아니며, 상기 배합사료와 배합하여 사용하더라도 본 발명에서 나타내는 기생충 감염 질환 예방 또는 개선 효과는 동일하게 발생된다.The compound feed commonly used in the feed composition for fish includes any one or more protein sources selected from fish meal, casein, bone meal, and shrimp powder, or any one or more carbohydrate sources selected from sweet potato starch, potato starch, wheat starch, But the present invention is not limited thereto. Even if it is used in combination with the compound feed, it is possible to prevent or ameliorate parasitic infectious diseases according to the present invention, The effect is the same.
또 다른 하나의 양태로서, 고삼 추출물, 이의 분획물 또는 이로부터 분리된 화합물을 유효성분으로 포함하는 조성물을 어류에 직접 또는 양식장에 투여하는 단계를 포함하는, 어류의 기생충 감염 질환 예방 또는 치료 방법을 제공한다.Another aspect of the present invention provides a method for preventing or treating parasitic infestation in fish, comprising the step of administering a composition containing an extract of rosemary extract, fraction thereof, or a compound isolated therefrom as an active ingredient, directly to a fish or to a fish farm. do.
또 다른 하나의 양태로서, 상기 고삼 추출물을 유효성분으로 포함하는 약학 조성물 또는 사료첨가제 조성물을 어류에 직접 또는 양식장에 투여하는 단계를 포함하는, 어류의 기생충 감염 질환 예방 또는 치료방법을 제공한다.Another aspect of the present invention provides a method for preventing or treating parasitic infestation in a fish, comprising the step of administering a pharmaceutical composition or feed additive composition containing the extract as an active ingredient directly to a fish or in a farm.
상기 어류의 기생충 감염 질환 예방 또는 치료 방법은, 상기 어류에 직접 또는 양식장에 투여하는 단계를 포함함으로써, 어류에 직접 또는 간접적으로 본 발명의 조성물이 전달되는 모든 수단을 포함할 수 있다.The method for preventing or treating a parasitic infectious disease of a fish may include any step in which the composition of the present invention is directly or indirectly delivered to a fish by including the step of administering the fish directly or in a farm.
구체적으로, 예컨데 어류에 직접적으로 주사하거나 급이함으로써 본 발명의 목적을 달성할 수도 있으며 약욕시키거나 섭식시키는 등의 수단을 통해서도 달성될 수 있다. 바람직하게는 유효농도의 조성물을 사료에 코팅하여 기생충 감염 질환으로 인한 병변을 가진 어류에 섭식시시키는 것일 수 있으나 이에 제한되지는 않는다. 이 때 본조성물의 투여량은 피처리 어류의 중량, 건강상태, 투여시간, 질환의 중증도에 따라 그 범위를 달리할 수 있다.Specifically, for example, the purpose of the present invention may be accomplished by directly injecting or feeding fish, and may also be accomplished by means of bathing or eating. Preferably, the effective concentration of the composition may be coated on the feed and fed to fish having lesions due to parasitic infectious disease, but is not limited thereto. At this time, the dose of the composition may vary depending on the weight of the fish to be treated, the health condition, the administration time, and the severity of the disease.
이하, 실시예를 통하여 본 발명의 효과에 대하여 설명하기로 한다.Hereinafter, effects of the present invention will be described by way of examples.
[실시예 1] : 고삼 추출물 준비[Example 1] Preparation of gosam extract
고삼 뿌리를 물로 완전히 세척하고 자연건조를 5 내지 7일 시킨 후, 절단하였다. 절단된 고삼 50 kg에 중량 대비 3배의 메탄올을 가하여 5일간 실온에서 추출한 후 여과하였다. 이를 감압 농축하고 동결건조시킨뒤 분말화하여 수득하였다.The roots were thoroughly washed with water, naturally dried for 5 to 7 days, and then cut. Three times as much methanol as weight was added to 50 kg of the cut gosam, and the mixture was extracted at room temperature for 5 days and then filtered. It was concentrated under reduced pressure, lyophilized and powdered.
[실시예 2] : 고삼의 sea lice 퇴치(기피)효과 [Example 2]: The effect of avoiding sea lice
Y-Tube choice camber를 사용하여 sea lice의 이동, 각각의 section에 머무르는 시간을 측정하여 sea lice 퇴치제에 대한 기피효과를 확인하였다.Using the Y-Tube choice camber, the movement of the sea lice and the time spent in each section were measured to confirm the avoidance effect of the sea lice repellent.
콘트롤 구간(Control section)에는 바닷물(clean sea water)을 흘려보내고, 물질 구간(compound section)에는 실험하고자 하는 물질을 흘려보낸다. 혼합 구간(Mixed section)은 콘트롤(control)과 물질(compound) 구간이 혼합되어 배수되는 구간이다.The control section is flushed with clean sea water and the compound section is flushed with the substance to be tested. Mixed section is a section where control and compound sections are mixed and drained.
바다이(Sea lice)는 혼합 구간(mixed section)이 시작되는 지점에 위치시키고 이동을 관찰하게 되며 실험 결과에 대한 해석은 시험물질이 흐르는 반대방향으로 즉, 콘트롤 구간(control section) 및 혼합구간(mixed section)으로 sea lice가 얼마나 역행하여 이동하고 머물러 있는지 시간을 분석하여 sea lice 퇴치효과를 확인하였다. (도 1 참조)The sea lice are located at the point where the mixed section starts and the movement is observed. The interpretation of the experimental results is carried out in the opposite direction in which the test substance flows, that is, the control section and the mixed section section), the sea lice effect was confirmed by analyzing how long the sea lice moved and stayed. (See Fig. 1)
상기 실험방법에 따라 콘트롤 구간에서는 바닷물을 흘려주고 물질(compound) 구간에는 본 발명의 물질을 흘려 바다이(sea lice)의 이동을 관찰한 결과는 도 2에서 나타난 바와 같다.According to the above experimental method, the movement of the sea lice was observed by flowing the seawater in the control section and flowing the substance of the present invention in the compound section, as shown in FIG.
도 2에서 Con.1은 대조구로 바닷물을 처리한 것, OH4_A는 고삼 0.5 mg/ml, OH4_B는 고삼 0.25 mg/ml를 각각 처리하여 바다이(sea lice) 퇴치(기피)효과를 나타낸 것으로서, Con.1에서는 Y-tube chamber 모든 구간에서 바닷물이 흐르므로 바다이(Sea lice)가 다른 구간으로 이동을 하지 않았으며, 고삼 0.5 mg/ml이 처리된 OH4_A에서 바다이(Sea lice)가 머문 시간(time spent)이 콘트롤 구간에 약 68%, 혼합구간(mixed section)에 약 20%, 물질구간(compound section)에 약 12% 이였으며, 고삼 0.25 mg/ml이 처리된 OH4_B에서 바다이(Sea lice)가 머문 시간(time spent)이 콘트롤 구간(control section)에 약 29%, 혼합구간(mixed section)에 약 24%, 물질구간(compound section)에 약 47%로 나타났습니다. 이는 고삼 농도 의존적으로 바다이(sea lice) 기피효과를 가진다는 것을 의미한다.In FIG. 2, Con.1 was treated with sea water as a control, OH4_A was treated with 0.5 mg / ml of gosam and OH4_B was treated with 0.25 mg / ml of salted ginseng respectively. 1, sea lice did not migrate to other sections because sea water flowed in all sections of the Y-tube chamber, while sea lice in OH4_A treated with 0.5 mg / In the control section, about 68%, about 20% in the mixed section, about 12% in the compound section, and in the OH4_B treated with ginseng 0.25 mg / ml, time spent was about 29% in the control section, about 24% in the mixed section, and about 47% in the compound section. This means that it has a sea lice avoidance effect depending on the concentration of red ginseng.
[실시예 3] : 고삼 대한 바다이(sea lice) 생존율[Example 3] Survival rate of Sea lice
바다이(sea lice)에 기피효과를 보이는 것으로 확인된 고삼의 바다이(sea lice)에 대한 퇴치효과를 확인하기 위해 24-well plate를 이용하여 실험을 수행하였다. 24-well plate에 바닷물(sea water), Sea lice와 유효물질을 농도별로 넣은 후 incubator에서 정치하였고, 실험에 사용한 모든 용액(solution)은 실험 동안 10℃로 유지하였으며 24 시간동안 incubation 후 sea lice의 상태를 관찰하여 sea lice 퇴치효과를 확인하였다.The experiment was carried out using a 24-well plate to confirm the effect of the sea lice on the sea lice. All the solutions used in the experiment were kept at 10 ° C during the experiment. After incubation for 24 hours, sea lice were added to each well. The effect of sea lice eradication was confirmed by observing the condition.
실험결과 도 3(a)에서 나타난 바와 같이 아무것도 처리하지 않은 대조구(control)의 경우 약 95% 이상의 sea lice가 생존하고 있지만, 고삼 추출물의 처리농도가 증가할수록 생존(Alive)하고 있는 개체수가 감소하면서 사멸(Dead)하는 개체수가 증가하였다. 또한 고삼 추출물의 농도가 0.31mg/ml인 경우 sea lice 개체수 50%(LD50)가 사멸(Dead)하는 효과를 보였으며, 0.12mg/ml에서 sea lice 개체수 50%(EC50)가 빈사(Moribund)상태가 되거나 유영이 저해되는 것으로 나타나 sea lice에 뛰어난 효과를 보였다.As shown in FIG. 3 (a), about 95% of the sea lice survived in the control without any treatment, but the number of alive individuals decreased as the concentration of the extract of the ginseng extract increased The number of dead species increased. In addition, when the concentration of ginseng extract was 0.31 mg / ml, 50% (LD50) of the sea lice population died, and 50% (EC50) of sea lice at 0.12 mg / And the swimming effect was inhibited and showed excellent effect on sea lice.
결론적으로 본 발명의 고삼 추출물 또는 이의 분획물이 항-바다이(sea lice) 활성을 가지므로 어류의 기생충 감염 질환 예방 또는 치료용, 기생충 퇴치, 방제에 사용될 수 있다. In conclusion, the ginseng extract or its fractions of the present invention have anti-sea lice activity and thus can be used for the prevention or treatment of parasitic infectious diseases of fish, the prevention of parasites, and the control thereof.
본 발명은 의약바이오 및 농축수산업 분야에서의 산업상 이용 가능성이 인정된다.INDUSTRIAL APPLICABILITY The present invention is recognized as an industrial applicability in the fields of pharmaceutical biotechnology and agriculture and fisheries.

Claims (12)

  1. 고삼(Sophora flavescens Aiton) 추출물 또는 이의 분획물을 유효성분으로 포함하는 어류 기생충 감염 질환의 예방 또는 치료용 약학 조성물A pharmaceutical composition for preventing or treating a fish parasitic infectious disease comprising an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredient
  2. 제1항에 있어서, 상기 고삼 추출물은 고삼 잎, 줄기, 뿌리, 열매 또는 전초인 것을 특징으로 하는 약학 조성물[Claim 2] The pharmaceutical composition according to claim 1, wherein the ginseng extract is ginseng leaf, stem, root, fruit or plant
  3. 제1항에 있어서, 상기 고삼 추출물은 마트린(matrine), 옥시마트린(oxymatrine), 아나기린(anagyrine), 소포라놀(sophoranol), 시티진(cytisine), 메틸시티진(methylcytisine), 쿠라리논(kurarinone), 잔토휴몰(xanthohumol), 노르안하이드로이카리딘(Noranhydroicaritin), 이소안하이드로이카리딘(isoanhydroicaritin), 글루코루데올린(glucoluteolin), 켐페롤-3-소포로시드(kaempferol-3-sophoroside), 헤니스테인(Heilsteine), 및 헤니스테인-4-람노실글리코시드(Heilsteine-4-rhamnosyl glucoside)으로 이루어진 군에서 하나 이상을 포함하는 것을 특징으로 하는 약학 조성물The method of claim 1, wherein the extract is selected from the group consisting of matrine, oxymatrine, anagyrine, sophoranol, cytisine, methylcytisine, xanthohumol, noranhydroicaritin, isoanhydroicaritin, glucoluteolin, kaempferol-3-sophoroside, and the like. Wherein the pharmaceutical composition comprises at least one compound selected from the group consisting of Heilsteine, and Heilsteine-4-rhamnosyl glucoside.
  4. 제1항에 있어서, 상기 고삼 추출물은 초음파추출, 열수추출 또는 용매추출된 것을 특징으로 하는 약학 조성물[Claim 2] The pharmaceutical composition according to claim 1, wherein the ginseng extract is subjected to ultrasonic extraction, hot water extraction or solvent extraction
  5. 제1항에 있어서, 상기 어류 기생충은 바다 이(sea lice), 시모토아 에시구아(Cymothoa Exigua), 백점충(Ichthyophthirius multifiliis), 비바기나충(Bivagina tai), 아나사키스(Anisakiasis), 디스토마(Distoma), 촌충(Tapeworm), 스쿠치카(Scutica), 베네데니아(Benedenia) 또는 고래회충(Anisakis Simplex)인 것을 특징으로 하는 약학 조성물The method according to claim 1, wherein the fish parasite is selected from the group consisting of sea lice, Cymothoa Exigua, Ichthyophthirius multifiliis, Bivagina tai, Anisakiasis, Distoma ), Tapeworm, Scutica, Benedenia or Anisakis Simplex.
  6. 제1항에 있어서, 상기 어류 기생충은 물이과(caligidae)인 것을 특징으로 하는 약학 조성물2. The pharmaceutical composition according to claim 1, wherein the fish parasite is caligidae.
  7. 제1항에 있어서, 상기 어류 기생충은 레페오프테이루스(Lepeophtheirus) 속 또는 칼리구스(Caligus) 속인 것을 특징으로 하는 약학 조성물The pharmaceutical composition according to claim 1, wherein the fish parasite is genus Lepeophtheirus or genus Caligus.
  8. 제7항에 있어서, 상기 어류 기생충은 레페오프테이루스 살모니스(Lepeoptheirus salmonis) 또는 칼리구스 로게르세르세이지(caligus rogercresseyi)인 것을 특징으로 하는 약학 조성물The pharmaceutical composition according to claim 7, wherein the fish parasite is Lepeoptheirus salmonis or caligus rogercresseyi.
  9. 제1항에 있어서, 상기 어류는 연어과 어류(Salmonidae)인 것을 특징으로 하는 약학 조성물The pharmaceutical composition according to claim 1, wherein the fish is Salmonidae.
  10. 고삼(Sophora flavescens Aiton) 추출물 또는 이의 분획물을 유효성분으로 포함하는 어류 기생충 감염 예방 또는 개선용 사료첨가제 조성물A feed additive composition for preventing or improving a fish parasitic infection comprising an extract of Sophora flavescens Aiton or a fraction thereof as an active ingredient
  11. 고삼(Sophora flavescens Aiton) 추출물 또는 이의 분획물을 유효성분으로 포함하는 어류 기생충 방제 또는 살충용 조성물A composition for controlling fish parasites or insecticidal compositions comprising Sophora flavescens Aiton extract or fractions thereof as an active ingredient
  12. 고삼(Sophora flavescens Aiton) 추출물 또는 이의 분획물을 유효성분으로 포함하는 조성물을 어류에 투입하여 어류의 기생충 감염을 예방 또는 치료하는 방법A method for preventing or treating parasitic infestation of fish by injecting a composition comprising Sophora flavescens Aiton extract or its fraction as an active ingredient into fish
PCT/KR2018/001249 2018-01-29 2018-01-29 Composition for preventing or treating parasitic infections in fish, comprising sophora flavescens aiton extract or fraction thereof WO2019146826A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
PCT/KR2018/001249 WO2019146826A1 (en) 2018-01-29 2018-01-29 Composition for preventing or treating parasitic infections in fish, comprising sophora flavescens aiton extract or fraction thereof
CA3088618A CA3088618C (en) 2018-01-29 2018-01-29 Compositions for preventing or treating fish parasitic infections, including extracts of sophora flavescens aiton or fractions thereof
NO20181070A NO345471B1 (en) 2018-01-29 2018-01-29 Pharmaceutical compositions for preventing or treating a sea lice disease comprising an extract of Sophora flavescens Aiton or fractions thereof.
GB2010954.2A GB2583664B (en) 2018-01-29 2018-01-29 Composition for preventing or treating sea lice infections in fish, comprising an extract of Sophora Flavescens Aiton or a fraction thereof

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CA3088618A1 (en) 2019-08-01
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CA3088618C (en) 2023-10-03
GB2583664A (en) 2020-11-04
NO345471B1 (en) 2021-02-22
GB202010954D0 (en) 2020-09-02

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