KR101866286B1 - A feed composition for preventing or treating parasitic infectious disease of fish comprising Reynoutria japonica extract - Google Patents

Abstract

The present invention relates to a feed additive composition, a pharmaceutical composition, an anti-parasitic composition, a feed comprising the feed additive composition for preventing or treating a parasitic infectious disease of a fish containing an effective ingredient of a carrageenan root extract, a method for breeding fish using the feed And a method of preventing or treating a parasitic infectious disease using the composition. Since the composition containing the extract of the present invention reduces the mortality of fishes by killing the parasites infected with fishes, the composition of the present invention can be widely used for improving the survival rate of parasitic infections and improving the fishery productivity due to improvement of fishery productivity. .

Description

Technical Field [0001] The present invention relates to a feed additive composition for preventing or treating a parasitic infectious disease of a fish comprising an extract of Aspergillus oryzae as an active ingredient,

The present invention relates to a feed additive composition, a pharmaceutical composition, an anti-parasitic composition, a feed comprising the feed additive composition for preventing or treating a parasitic infectious disease of a fish containing an effective ingredient of a carrageenan root extract, a method for breeding fish using the feed And a method of preventing or treating a parasitic infectious disease using the composition.

Fish farming is an innovative alternative to the depletion of fishery resources. Since the mid 1990s, the government has been actively promoting the main fish species such as flounder, rockfish, shrimp and red sea bream. Among them, , Which accounts for a large portion of the total fish production. However, the current rate of mulching in Korea is more than 20% per year, and the main cause of this is the incidence of intractable diseases, mixed infections (viruses, bacteria, parasites)

There are viral septicemia, burnavirus, vibriosis, sliding bacteria, scutia, and tricodinae, which are infected and damaged by cultured flounder (10 cm) 30 cm) have been infected and are suffering from diseases such as lymphocystisis, Rappovirus, Edwad disease, Streptococcus, and Bacillus thuringiensis.

Especially, in case of infestation of Sututika fish in aquaculture fish, the damage is significant and causes massive mortality, which is a big problem in the aquaculture industry. As a parasitic infectious disease, scuticacidosis is a disease that predominantly occurs in seawater fish cultivated in the aquaculture tanks on the land or in marine cages, especially in flounder, and is a parasite belonging to Scuticociliatids , a species of protozoa of protozoa It is caused by invading the subcutaneous tissues of the flounder and causing ulcers. Sututika saccharides are infected irrespective of their size, including fishes, fishes, and sexually transmitted fishes, and the amount of dead fish caused by Sututika fish is about 10% of the total amount of dead fish.

At present, the occurrence of these diseases is firstly observed in the fry of the seedling production field, and then after the frying period, the sutuchakugi (January to February), Edwad disease (March to September) (April to October) and lymphocystisis (January to December) appear as single or mixed infections.

As a treatment method of Sukutika, which has been conventionally used, there has been used a method of bathing Sukutika with 100-150 ppm of formalin for about 1 hour when parasitized on the outside such as body surface, gills and fins. The above-mentioned formalin is prepared by dissolving formaldehyde, a typical carcinogen, in water to a concentration of 35 to 38%. It is widely used as a disinfectant and preservative. In Korea, it is classified as a poison according to the Water Quality Preservation Act (Article 29), the Toxic Chemicals Management Act (Article 15), and the Waste Management Act (Article 25) Are classified as hazardous substances according to the Air Quality Conservation Act (Article 2).

Therefore, the use of formalin as a remedy for Sukhtika spp. Has become more difficult to use in domestic farms, and it is necessary to develop a pharmaceutical composition to replace it. However, a specific alternative composition has not been developed yet. It is urgent to develop a remedy for Sucutica spp.

In this connection, compositions derived from extracts of Rhus verniciflua bark and Polysiphonia morrowii Harvey have been reported to exhibit antiscuctic activity (Korean Patent No. 10-1117754, No. 10-1149382 number).

Under these circumstances, the present inventors have focused on natural substances which can prevent or treat a parasitic infectious disease caused by a suture of a fish or the like, unlike formalin, which is not toxic. Accordingly, it is possible to provide a feed composition, a pharmaceutical composition, and an anti-parasitic composition having an activity of inhibiting the antiscuctica from the natural product-derived Hojangun extract and having excellent inhibitory activity against the parasitic infectious disease of fish using the same And confirmed the present invention.

It is an object of the present invention to provide a feed additive composition for preventing or treating a parasitic infectious disease of a fish, which comprises an extract of Aspergillus oryzae as an active ingredient.

It is another object of the present invention to provide a feed comprising the feed additive composition.

Yet another object of the present invention is to provide a method for breeding fish comprising feeding the feed to a fish.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating a parasitic infectious disease of a fish containing an extract of Aspergillus oryzae as an active ingredient.

Another object of the present invention is to provide a method for preventing or treating a parasitic infectious disease of a fish, comprising the step of administering a composition containing an extract of Aspergillus oryzae as an active ingredient directly to a fish or a farm.

Another object of the present invention is to provide an anti-parasitic composition comprising an extract of Aspergillus oryzae as an active ingredient.

This will be described in detail as follows. On the other hand, each description and embodiment disclosed in the present invention can be applied to each other description and embodiment. That is, all combinations of various elements disclosed in the present invention fall within the scope of the present invention. Further, the scope of the present invention is not limited by the detailed description described below.

In order to accomplish the above object, the present invention provides a feed additive composition for preventing or treating a parasitic infectious disease of fish comprising an extract of Aspergillus oryzae as an active ingredient.

The term " Reynoutria japonica HOUTT. "As used in the present invention is a perennial herbaceous plant belonging to the genus Liliaceae. It is grown in mountains and branches of each country. The stem is empty, Purple spots are scattered. Hojangun is used as palliative, diuretic and general economy, and is used as sedative in the private sector.

However, the anti-parasitic activity of Hojangun has not been known yet. Accordingly, the present invention has revealed that the extract of Paralichthys olivaceus has an antiparasitic activity against parasites.

As used herein, the term " Hojangun extract " means all the extracts extracted from Hojanggang.

Specifically, the callus root extract may be isolated from a natural substance, specifically, callus root. It can be extracted by extracting various organ, root, stem, leaf, flower as well as plant tissue culture of natural, hybrid, and variant plants.

In the present invention, Hojangun can be commercially purchased, purchased, or harvested or cultivated in nature. In addition, it may be used as it is, or it may be used as it is dried by being dried as it is, or it may be used in dried dry form by removing its husk, .

In the present invention, the Hojokgung extract can be obtained by extracting with water, a C ₁ to C ₄ lower alcohol or a mixed solvent thereof. Concretely, water having a volume of about 2 to 10 times, particularly 2 to 5 times the weight of the root of the root thereof, lower alcohol such as ethanol and methanol, more specifically ethanol at 20 to 50 ° C, more specifically 25 The mixture was filtered through a filter paper, and the filtrate was removed by rotary vacuum evaporator. The residue was subjected to vacuum freeze drying and hot air drying Can be obtained through this method.

In addition, the callus root extract may contain at least one of an extract obtained by extraction treatment, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, and a controlled preparation or purified product thereof.

In the present invention, the fish may be collectively referred to as any fish to which a parasitic infectious disease can occur, and may be specifically aquaculture fish. More specifically, it can be divided into two groups : Paralichthys olivaceus , Seriola quinqueradiata , Pagrus major , Takifugu rubripes , Seriola aureovittata , Oncorhynchus masou , Oncorhynchus mykiss , Seriola dumerili), horse mackerel (Trachurus japonicus), sunspot line mackerel (Pseudocaranx dentex), grouper (Epinephelus septemfasciatus), bluefin tuna (Thunnus thynnus), carp (Cyprinus carpio), zebrafish (Danio rerio), catfish (Silurus asotus), But is not limited to, a fish selected from the group consisting of Clarias gariepinus , Oreochronis niloticus , Oncorhynchus keta , Salmo salar and Oryzias latipes . In one embodiment of the present invention, an experiment was conducted using a flounder.

The term " parasitic infectious disease "as used herein is collectively referred to as a disease caused by a parasitic infection. Specifically, the parasitic infectious diseases can be selected from the group consisting of Ichthyophthirius multifillis, Cryptocaryon irritans, Trichodina, Chilodonella, Ambiphrya, Apiosoma, Epistylis Epistylis, Carchesium, Scuticociliatids, and more specifically infectious diseases caused by Scuticociliatids , but the present invention is not limited thereto.

These parasites cause parasitic diseases such as scuticacid, reticulatum, reticulatum, and tricodinae, which cause serious damage to aquaculture fish. In particular, Parasitic injuries are often caused by large damage.

The Sututika spp. Is a parasitic ciliate in aquaculture, and it often inflicts great damage on the fry in cultivation in Korea and Japan. Sututika worms cause scuticacidosis, and are infected with seawater fish cultivated in aquaculture aquaculture or marine cages, especially artificial seedlings and fishes of flounder. The infected area also causes deep infection to the body surface, fins, and liver, blood vessels and brain tissues, and mass destruction of flounder mostly through destruction of plastic connective tissue. Unlike other parasites of fish, Sucutica spp. Is invasive to the brain tissue through blood vessels and is parasitic.

The term "prevention" used in the present invention refers to any action that inhibits or delays the onset of parasitic infectious disease of a fish by administration of a composition comprising the extract of Aspergillus maximus according to the present invention as an active ingredient.

The term "treatment" used in the present invention refers to any action that improves or alters the symptoms of the disease by administering a composition comprising the extract of Callus root according to the present invention as an active ingredient.

In the examples of the present invention, it was confirmed that Sucutika spp. Was killed by expanding and dissolving the cell membrane of Sucutika spp. (Table 1 and Fig. 1), and the flounder fed with Sophora spp. And the acute mortality rate was significantly reduced (Fig. 2).

Therefore, the feed additive composition of the present invention containing the callus root extract may be usefully used for the prevention or treatment of parasitic infectious diseases.

The term "feed additive " as used in the present invention refers to a substance added to feed in a minor amount for nutritional or specific purposes. In the present invention, a substance added for the purpose of preventing, improving or treating a parasitic infectious disease of fish .

The term "feed" as used in the present invention means any natural or artificial diet, single meal, or the like ingredients for eating, ingesting, digesting, or suitable for the animal. Specifically, all the foods supplied to the fish may be used in a generic sense, but the present invention is not limited thereto.

The feed additive composition of the present invention may contain an extract of Hojokgang, as an active ingredient, for the prevention or treatment of parasitic infectious diseases of fish, and the extract may exist in powder form.

The feed additive composition of the present invention can be prepared by adding the root gang root extract in an appropriate effective concentration range according to various production methods known in the art. Specifically, the feed additive composition of the present invention may include, but is not limited to, 0.01 to 100% by weight of the total feed additive composition, specifically 0.01 to 50% by weight of the total feed additive composition.

The feed additives of the present invention may further comprise ingredients that can be added to conventional feeds. For example, in addition to the components described above, the fish feed additive may further contain organic acids such as citric acid, fumaric acid, adipic acid, and lactic acid, phosphates such as potassium phosphate, sodium phosphate, and polymerized phosphate, polyphenol, catechin, tocopherol, vitamin C, Green tea extract, chitosan, and tannic acid. If necessary, other conventional additives such as anti-influenza agents, buffers, and antibacterial agents may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be added to formulate into injectable formulations, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

The feed additive of the present invention corresponds to an auxiliary feed in the feed control method and is a mineral preparation such as sodium hydrogencarbonate, bentonite, magnesium oxide and complex mineral, a mineral preparation such as zinc, copper, cobalt and selenium, , Vitamins such as vitamin E, vitamin A, D, E, nicotinic acid and vitamin B complex, protective amino acids such as methionine and lysic acid, protective fatty acids such as calcium salt of fatty acid, probiotics (lactic acid bacteria), yeast culture, Fermented foods and the like, yeast preparations and the like may be further included.

The feed additive composition of the present invention and the feed containing the same include marine protein (fish meal or krill mill), vegetable protein (soybean powder, wheat gluten, corn gluten, lupine powder, pea or sunflower powder) One or more energy sources selected from the group consisting of one or more proteins selected from the group consisting of fish oil (squid liver oil) or vegetable oil (rapeseed oil, soybean oil, soybean oil), vitamin mixture and mineral mixture, It is not limited.

The feed additive composition of the present invention can be applied to a large number of animal diets, i.e. feeds, including mammals, poultry, fish and crustaceans.

In the method for mixing a feed for fish comprising the feed additive composition according to the present invention, the feed additive composition may be mixed with an animal feed in an amount of about 1 g to 500 g, specifically 10 g to 100 g, per 1 kg on a dry weight basis. In addition, the feed mixture may be thoroughly mixed and then fed into a mesh, or may be subjected to pelleting, expansion, and extrusion through additional processing steps.

The feed additives for fish feed can be mixed directly with the feed of the fish or can be easily administered separately from the feed alone or in combination with other ingredients by oral administration, injection or transdermal route. The dosage varies depending on the fish body weight, health condition, diet, method of administration and severity of disease. As is well known in the art, the daily dose is about 0.01 to 20 mg / g, specifically 0.05 to 10 mg / g, and can be administered once a day or divided into several times a day.

The feed additive of the present invention can be added to the feed for the purpose of enhancing resistance of the fish to parasitic infectious diseases. When the feed additive of the present invention is used as a feed additive, the feed additive may be directly added or used together with other ingredients, and suitably used according to a conventional method.

The dosage form of the feed additive may be prepared in an immediate release or sustained release formulation in combination with a non-toxic pharmaceutically acceptable carrier. Such edible carriers may be corn starch, lactose, sucrose and bean-coated propylene glycol. In the case of solid carriers, they may be used as tablets, powders, and troches. In the case of liquid carriers, they may be in the form of syrups, liquid suspensions, emulsions, and solutions. In addition, the agent may contain preservatives, lubricants, solution promoters, stabilizers, and may contain other inflammatory disease ameliorating agents and substances useful for the prevention of parasitic infections.

According to another aspect of the present invention, there is provided a feed comprising the feed additive composition.

The feed additive and feed are as described above.

Since the feed additive composition comprising the callus root extract of the present invention can be used for the purpose of preventing or treating a parasitic infectious disease of fish, the feed comprising the same can also be used for the prevention or treatment of the disease.

The feed containing the root extract of the present invention as an active ingredient can be prepared in various forms known in the art, and concretely, it can include concentrated feed, forage and / or special feed.

Concentrated feeds include seeds containing cereals such as wheat, oats, and corn, and by-products such as rice bran, wheat bran, barley, etc., (Meat powder) such as residual starch residue, which is the main component of starch residue, which is the byproduct of starch residue, sweet potato and potato, which is a byproduct obtained from the sweet potato and sweet potato, and casein from skim milk and skim milk. Yeast, chlorella, seaweed, and the like, such as dry fescue, which is a balance of the whey produced during the production.

Roughage includes root vegetables such as wild grasses, grasses and fodder, raw turnip for feed, turnip for feed, feed bit, turnip root, Silage, which is filled with lactic acid fermented, hay, dried hay, herbaceous straw, and leaves of leguminous plants.

Special feeds include mineral feeds such as peelings, rock salt, urea feeds such as urea and its derivatives diuret isobutane, supplemented with ingredients that are insufficient when mixed with natural feed ingredients, or mixed feeds to improve the storage stability of the feed. Feed additives, dietary supplements, etc., which are added in small quantities. The dietary supplement is, for example, a composition containing an agent for providing a therapeutic agent or an extinguishing agent to an animal, and refers to a composition that is normally ingested in addition to ordinary diets, though not a caloric intake source, that is, an energy source of a living body.

The feed composition comprising the feed additive composition according to the present invention is prepared by mixing the feed additive composition with the feed in an amount of about 1 g to 500 g, more specifically 5 g to 50 g, per 1 kg on a dry weight basis. In addition, the feed mixture may be thoroughly mixed and then fed into a mesh, or may be subjected to pelletization, expansion and extrusion processes through further processing steps.

According to another aspect of the present invention, there is provided a method of breeding a fish, comprising feeding the feed to a fish.

The feed and fish are as described above.

Since the feed containing the callus root extract of the present invention can be used for the purpose of preventing or treating parasitic infectious diseases of fishes, the method of breeding of the present invention including the step of feeding the feeds can prevent or treat the diseases. It is possible to breed healthy by minimizing the mortality of fish.

In the present invention, the step of feeding the feed to the fish may include the step of directly or indirectly feeding the feed to the fish, or all the means by which the composition of the present invention is delivered to the fish.

At this time, the dose of the feed may be varied depending on the weight of the fish to be treated, the health condition, the time of administration, and the like, and may be administered according to a conventional dietary method.

In the examples of the present invention, it was confirmed that Sucutika spp. Was killed by expanding and dissolving the cell membrane of Sucutika spp. (Table 1 and Fig. 1), and the flounder fed with Sophora spp. And the acute mortality rate was significantly reduced (Fig. 2).

Therefore, the breeding method of the present invention using the feed containing the Hojokgang extract can efficiently breed fish through the prevention or treatment of parasitic infectious diseases.

In another aspect of the present invention, the present invention provides a pharmaceutical composition for preventing or treating a parasitic infectious disease of a fish, which comprises an extract of Aspergillus oryzae as an active ingredient.

As described above, the callus root extract, fish, parasitic infectious disease, prevention and treatment are as described above.

The term "pharmaceutical composition" used in the present invention means a preparation for the purpose of preventing or treating disease, and can be formulated into various forms according to ordinary methods. For example, it can be formulated into oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions and syrups, and can be formulated in the form of external preparations, suppositories, and sterilized injection solutions. However, the composition of the present invention may be provided in the form of a bathing agent for the purpose of treating diseases of fishes.

In addition, each of the formulations may be further prepared by incorporating pharmaceutically acceptable carriers such as buffers, preserving agents, wetting agents, solubilizing agents, isotonic agents, stabilizers, bases, excipients, lubricants and the like in the art .

Meanwhile, the pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. The term "pharmaceutically effective amount " of the present invention means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to veterinary treatment and not causing side effects, , The severity, the activity of the drug, the sensitivity to the drug, the method of administration, the time of administration, the route of administration and the rate of release, the duration of the treatment, the factors including the combined or concurrently used drugs, and other factors well known in the veterinary arts.

In the examples of the present invention, it was confirmed that Sucutika spp. Was killed by expanding and dissolving the cell membrane of Sucutika spp. (Table 1 and Fig. 1), and the flounder fed with Sophora spp. And the acute mortality rate was significantly reduced (Fig. 2).

Therefore, the pharmaceutical composition of the present invention comprising the callus root extract can be usefully used for the prevention or treatment of parasitic infectious diseases.

In accordance with another aspect of the present invention, there is provided a method for preventing or treating a parasitic infectious disease in a fish, comprising the step of administering a composition comprising an extract of Aspergillus oryzae as an active ingredient, .

As described above, the callus root extract, fish, parasitic infectious disease, prevention and treatment are as described above.

The method for preventing or treating a parasitic infectious disease of the fish may include any step in which the composition of the present invention is directly or indirectly delivered to the fish by including the step of administering the fish directly or in a farm.

Specifically, the purpose of the present invention can be achieved by directly injecting or feeding fish, and can also be accomplished through means such as bathing or eating. At this time, the dose of the composition may be varied depending on the weight of the fish to be treated, the health condition, the administration time, and the severity of the disease.

In another aspect of the present invention, the present invention provides an anti-parasitic composition comprising an extract of Aspergillus oryzae as an active ingredient.

The callus root extract has been described above.

As used herein, the term " anti-parasitic "means exhibiting an antimicrobial activity against parasites, and the anticancer composition can be used for antimicrobial use against parasites.

At this time, the parasites may be scutica, but are not limited thereto.

The composition of the present invention containing the callus root extract has an advantage of showing a disinfecting effect without harming the human body or fish as compared with an antiparasitic substance such as formalin, which is based on natural products.

On the other hand, formalin is a powerful sterilizing disinfectant at low cost, but it is not only a toxic substance that emits irritating odor, but it is also classified as a carcinogenic substance and strictly limits its use in the Marine Pollution Control Law, Pharmaceutical Affairs Law, and Food Sanitation Law.

In addition, the antiviral composition of the present invention can be used in combination with other antimicrobial agents to maximize its effect.

In the examples of the present invention, it was confirmed that the callus root extract was killed by expanding and dissolving the cell membrane of Sutica spp. (Table 1 and Fig. 1).

Accordingly, the composition containing the callus root extract may be useful as an antiparasitic composition for antimicrobial use against parasites.

Since the composition containing the extract of the present invention reduces the mortality of fishes by killing the parasites infected with fishes, the composition of the present invention can be widely used for improving the survival rate of parasitic infections and improving the fishery productivity due to improvement of fishery productivity. .

Figure 1 schematically depicts the phenotype of Scuticociliatids during the treatment of the extracts of the carrageenan . The phenotype at 10 minutes and 4 hours after treatment was expressed as x40 or x200.
Fig. 2 is a graph comparing the initial mortality rate of Sucutika spp.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of callus root extract and complex agent

The callus root used in the present invention is generally easily collected from nature, In order to obtain the extract efficiently, it was pulverized in the form of filament.

(2 ㎏) was extracted with organic solvent, ethanol for food or methanol (20 L) for about 5 to 10 days at room temperature. The extracts were filtered and concentrated under reduced pressure to prepare callus root extract.

Example 2. Identification of anti-parasitic effect of carotid root extract

Example 2-1. Identification of parasitic killing effect

First, in order to examine the antiparasitic effect of the composition containing the extract of Callus roots of Example 1 , scuticociliatids ( Scuticociliatids ) in a number of 10,000 cells / well were dispensed into a 96-well plate, The extracts were treated by concentration.

Specifically, 90 占 퐇 of MEM containing 10,000 healthy scuticacid cells exhibiting good morphology and vigorous mobility was dispensed into each well, and then the final concentration of the callus root extract contained in the well was 1 mg / ml, 10 μl of the MEM medium containing the callus root extract, which was adjusted to be 100 μg / ml, 50 μg / ml, 10 μg / ml and 5 μg / ml, was added to the wells to make a final volume of 100 μl. After 10, 4, and 20 hours, the extracts were examined by microscopy to evaluate the antimicrobial efficacy of Sukutika worms by confirming their morphology, motility, aggregation and cell membrane lysis. Thereafter, the antiparasiticidal effects of the extracts of the carotovorhinopsis lanceolata were evaluated as strong (+++ / ***), heavy (++ / **), weak (+ / *) and no effect - / -).

As a result, it was confirmed that the cellulase contents were aggregated by expanding and dissolving the cell membrane in 10 minutes when most of the Sukutika extract was treated, and the mobility of the Sukitika crab at 10 μg / And it was confirmed that more than half of the Suktika larva died to reach the inactivation state due to cell membrane expansion. After 20 hours, it was confirmed that most of the scuticacid was aggregated even at the lowest concentration of 5 / / ml, and was inactivated due to cell membrane expansion (FIG. 1 and Table 1).

Hojangun extract Not processed 1 mg / ml 100 [mu] g / ml 50 [mu] g / ml 10 [mu] g / ml 5 [mu] g / ml Processing time 10 minutes - - +++ *** ++ *** + ** - ** - - 4 hours - - +++ *** ++ *** + *** + ** - ** 20 hours - - +++ *** +++ *** +++ *** ++ *** ++ * † + low level of aggregation; ++ indicates moderate coagulation; +++ is a high level of aggregation
‡ * indicates a low level of expansion; ** is a medium level swelling; *** a high level of expansion; - No effect

Therefore, it was found that the use of the callus root extract as a feed additive composition can effectively prevent and treat parasite infection.

Example 2-2. Determination of the reduction effect of flounder on dead fish

First, in order to confirm the anti-parasitic effect of the composition containing the extracts of Example 1, the composition of the present invention was applied to the flounder cut in Sukutika pupa, and the initial mortality of the flounder was confirmed.

Flounder (9.0 ± 0.8cm / 6.3 ± 1.4g) consisted of 200 animals per group. When commercial pelleted feed was fed, more than 5% of the feed was fed per day. To the 60 g diets, 1% of carotene extract was added to the flounder in the laboratory and farm. Then 15 rats were randomly selected for each group, and acute death was induced by intraperitoneal injection of 5 × 10 ⁶ cells of Sutuca cordata, and the survival of the flounder was observed for 72 hours.

As a result, 72 hours after the induction of acute death, 80% of the initial mortality rate of the control group of Sucutika spp. Showed the initial mortality rate, 45% and 50% of the group treated with 0.5% and 1% . That is, it was confirmed that the group administered with 0.5% and 1% of the extract of Callus roots improved the mortality due to the scuticaccharide and the survival rate was increased as compared with the group without the extract (FIG. 2).

Therefore, it was found that when the extract of the present invention is used as a feed additive composition, the parasite infection can be effectively prevented and treated and, as a result, the mortality rate can be reduced.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all aspects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Claims (6)

delete A feed additive composition for prevention or treatment of a parasitic infectious disease of fish, comprising an extract of Aspergillus oryzae as an active ingredient, wherein the parasite is scuticaccharide.
A feed comprising the feed additive composition of claim 2.
The present invention relates to a pharmaceutical composition for preventing or treating a disease caused by infestation of fish,
A method for preventing or treating a scuticacid infectious disease in a fish, comprising the step of administering a composition containing an extract of Aspergillus oryzae as an active ingredient directly to a fish or in a farm.
A composition for inhibiting Hanskutica, comprising an extract of Aspergillus oryzae as an active ingredient.

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