NO324880B1 - Tuberkulose-antigener og fusjonsproteiner omfattende disse, DNA som koder for antigenene og ekspresjonsvektorer og vertsceller omfattende dette DNA, samt farmasoytiske sammensetninger og vaksiner for immunoterapi og diagnose av tuberkulose. - Google Patents
Tuberkulose-antigener og fusjonsproteiner omfattende disse, DNA som koder for antigenene og ekspresjonsvektorer og vertsceller omfattende dette DNA, samt farmasoytiske sammensetninger og vaksiner for immunoterapi og diagnose av tuberkulose. Download PDFInfo
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- NO324880B1 NO324880B1 NO19995689A NO995689A NO324880B1 NO 324880 B1 NO324880 B1 NO 324880B1 NO 19995689 A NO19995689 A NO 19995689A NO 995689 A NO995689 A NO 995689A NO 324880 B1 NO324880 B1 NO 324880B1
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- tuberculosis
- seq
- polypeptide
- dna
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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| US09/073,010 US6613881B1 (en) | 1997-05-20 | 1998-05-05 | Compounds for immunotherapy and diagnosis of tuberculosis and methods of their use |
| PCT/US1998/010407 WO1998053075A2 (en) | 1997-05-20 | 1998-05-20 | Compounds for immunotherapy and diagnosis of tuberculosis and methods of their use |
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| NO995689D0 NO995689D0 (no) | 1999-11-19 |
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| NO19995689A NO324880B1 (no) | 1997-05-20 | 1999-11-19 | Tuberkulose-antigener og fusjonsproteiner omfattende disse, DNA som koder for antigenene og ekspresjonsvektorer og vertsceller omfattende dette DNA, samt farmasoytiske sammensetninger og vaksiner for immunoterapi og diagnose av tuberkulose. |
| NO20075901A NO20075901L (no) | 1997-05-20 | 2007-11-15 | Forbindelser for immunterapi og diagnose av tuberkolose og metoder for deres anvendelse |
| NO20075902A NO20075902L (no) | 1997-05-20 | 2007-11-15 | Forbindelser for immunoterapi og diagnose av turberkolose og metoder for deres anvendelse |
| NO20075900A NO20075900L (no) | 1997-05-20 | 2007-11-15 | Forbindelser for immunterapi og diagnose av tuberkulose og metoder for deres anvendelse |
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| NO20075901A NO20075901L (no) | 1997-05-20 | 2007-11-15 | Forbindelser for immunterapi og diagnose av tuberkolose og metoder for deres anvendelse |
| NO20075902A NO20075902L (no) | 1997-05-20 | 2007-11-15 | Forbindelser for immunoterapi og diagnose av turberkolose og metoder for deres anvendelse |
| NO20075900A NO20075900L (no) | 1997-05-20 | 2007-11-15 | Forbindelser for immunterapi og diagnose av tuberkulose og metoder for deres anvendelse |
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| EP (4) | EP2172551A3 (https=) |
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| PT (1) | PT1012293E (https=) |
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| US6991797B2 (en) | 1993-07-02 | 2006-01-31 | Statens Serum Institut | M. tuberculosis antigens |
| US6290969B1 (en) * | 1995-09-01 | 2001-09-18 | Corixa Corporation | Compounds and methods for immunotherapy and diagnosis of tuberculosis |
| US6592877B1 (en) | 1995-09-01 | 2003-07-15 | Corixa Corporation | Compounds and methods for immunotherapy and diagnosis of tuberculosis |
| US6458366B1 (en) | 1995-09-01 | 2002-10-01 | Corixa Corporation | Compounds and methods for diagnosis of tuberculosis |
| US6982085B2 (en) | 1997-04-02 | 2006-01-03 | Statens Serum Institut | TB diagnostic based on antigens from M. tuberculosis |
| US7037510B2 (en) | 1997-04-18 | 2006-05-02 | Statens Serum Institut | Hybrids of M. tuberculosis antigens |
| EP1003850B1 (en) | 1997-06-06 | 2009-05-27 | The Regents of the University of California | Inhibitors of dna immunostimulatory sequence activity |
| CA2319380A1 (en) * | 1997-11-10 | 1999-05-20 | Statens Serum Institut | Nucleic acid fragments and polypeptide fragments derived from m. tuberculosis |
| DK1144447T3 (da) * | 1998-11-04 | 2010-02-08 | Isis Innovation | Dianostisk tuberkulosetest |
| US7083796B2 (en) * | 2000-06-20 | 2006-08-01 | Corixa Corporation | Fusion proteins of mycobacterium tuberculosis |
| US20030027774A1 (en) * | 1999-03-18 | 2003-02-06 | Ronald C. Hendrickson | Tuberculosis antigens and methods of use therefor |
| US8143386B2 (en) * | 1999-04-07 | 2012-03-27 | Corixa Corporation | Fusion proteins of mycobacterium tuberculosis antigens and their uses |
| JP5010079B2 (ja) | 1999-07-13 | 2012-08-29 | スタテンズ セーラム インスティテュート | マイコバクテリウム・ツベルクローシスｅｓａｔ−６遺伝子ファミリーベースの結核ワクチン及び診断法 |
| EP1229931A4 (en) | 1999-10-07 | 2003-05-28 | Corixa Corp | FUSION PROTEINS FROM MYCOBACTERIUM TUBERCULOSIS |
| US6316205B1 (en) | 2000-01-28 | 2001-11-13 | Genelabs Diagnostics Pte Ltd. | Assay devices and methods of analyte detection |
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| AU2001250294A1 (en) * | 2000-04-19 | 2001-10-30 | Statens Serum Institut | Tuberculosis antigens and methods of use thereof |
| US7026465B2 (en) * | 2002-02-15 | 2006-04-11 | Corixa Corporation | Fusion proteins of Mycobacterium tuberculosis |
| US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
| WO2005118884A1 (en) * | 2004-05-28 | 2005-12-15 | The United States Of America As Represented By The Secretary Of The Navy | A method for the rapid diagnosis of infectious disease by detection and quantitation of microorganism induced cytokines |
| US8475735B2 (en) * | 2004-11-01 | 2013-07-02 | Uma Mahesh Babu | Disposable immunodiagnostic test system |
| CN102220357B (zh) * | 2004-11-16 | 2013-12-25 | 克鲁塞尔荷兰公司 | 包含重组病毒载体的多价疫苗 |
| WO2006117538A2 (en) * | 2005-04-29 | 2006-11-09 | Fusion Antibodies Limited | Assays for diagnosis of tuberculosis and uses thereof |
| EP1910410B1 (en) | 2005-07-01 | 2011-10-26 | Forsyth Dental Infirmary for Children | Tuberculosis antigen detection assays and vaccines |
| WO2007041539A1 (en) * | 2005-09-30 | 2007-04-12 | Transcutaneous Technologies, Inc. | Iontophoresis apparatus and method for the diagnosis of tuberculosis |
| EP2441469A1 (en) | 2006-03-14 | 2012-04-18 | Oregon Health and Science University | Methods for producing an immune response to tuberculosis |
| AU2009273130B2 (en) * | 2008-07-25 | 2014-10-16 | Glaxo Group Limited | Novel compositions and methods |
| AU2009273132B2 (en) | 2008-07-25 | 2015-09-03 | Glaxo Group Limited | The tuberculosis Rv2386c protein, compositions and uses thereof |
| CA2731549C (en) | 2008-07-25 | 2018-07-17 | Glaxo Group Limited | Compositions and methods for treating tuberculosis |
| BR112012018669A2 (pt) | 2010-01-27 | 2017-09-05 | Glaxosmithkline Biologicals Sa | Proteína rv3616c modificada, uso de uma proteína rv3616c modificada, polinucleotídeo, uso de um polinucleotídeo, composição farmacêutica, composição imunogênica, proteína de fusão, e, polipeptídeo. |
| GB201012072D0 (en) * | 2010-07-19 | 2010-09-01 | Sec Dep For Environment Food A | Diagnostic reagents |
| WO2012177656A2 (en) | 2011-06-19 | 2012-12-27 | Abogen, Inc. | Devices, solutions and methods for sample collection |
| US12038434B2 (en) | 2015-02-09 | 2024-07-16 | DNA Genotek, Inc. | Devices, solutions and methods for sample collection related applications, analysis and diagnosis |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4751180A (en) | 1985-03-28 | 1988-06-14 | Chiron Corporation | Expression using fused genes providing for protein product |
| US4689397A (en) | 1985-08-12 | 1987-08-25 | Scripps Clinic And Research Foundation | Synthetic polypeptides for detecting mycobacterial infections |
| US4935233A (en) | 1985-12-02 | 1990-06-19 | G. D. Searle And Company | Covalently linked polypeptide cell modulators |
| US4879213A (en) | 1986-12-05 | 1989-11-07 | Scripps Clinic And Research Foundation | Synthetic polypeptides and antibodies related to Epstein-Barr virus early antigen-diffuse |
| US4952395A (en) | 1987-02-26 | 1990-08-28 | Scripps Clinic And Research Foundation | Mycobacterial recombinants and peptides |
| US4976958A (en) * | 1987-02-26 | 1990-12-11 | Scripps Clinic And Research Foundation | Mycobacterial recombinants and peptides |
| US6037135A (en) * | 1992-08-07 | 2000-03-14 | Epimmune Inc. | Methods for making HLA binding peptides and their uses |
| US5330754A (en) | 1992-06-29 | 1994-07-19 | Archana Kapoor | Membrane-associated immunogens of mycobacteria |
| EP0736098A1 (en) * | 1993-12-23 | 1996-10-09 | Agresearch New Zealand Pastoral Agriculture Research Institute, Ltd. | Mycobacteria virulence factors and a method for their identification |
| IE960132A1 (en) * | 1995-03-13 | 1996-09-18 | Astra Ab | New DNA molecules |
| AU727602B2 (en) | 1995-09-01 | 2000-12-14 | Corixa Corporation | Compounds and methods for immunotherapy and diagnosis of tuberculosis |
| CA2230927A1 (en) | 1995-09-01 | 1997-03-13 | Corixa Corporation | Compounds and methods for diagnosis of tuberculosis |
| US6290969B1 (en) * | 1995-09-01 | 2001-09-18 | Corixa Corporation | Compounds and methods for immunotherapy and diagnosis of tuberculosis |
| BR9712298A (pt) * | 1996-10-11 | 2000-10-24 | Corixa Corp | Polipeptìdeo, molécula de dna, vetor de expressão recombinante, célula hospedeira, processo para detectar infecção por m. tuberculosis em uma amostra biológica, kit diagnóstico, anticorpos monoclonal, e, policlonal, e, proteìna de fusão |
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