NO20082224L - En mortierella alpina C16/18 fettsyreelongase - Google Patents

En mortierella alpina C16/18 fettsyreelongase

Info

Publication number
NO20082224L
NO20082224L NO20082224A NO20082224A NO20082224L NO 20082224 L NO20082224 L NO 20082224L NO 20082224 A NO20082224 A NO 20082224A NO 20082224 A NO20082224 A NO 20082224A NO 20082224 L NO20082224 L NO 20082224L
Authority
NO
Norway
Prior art keywords
fatty acid
acid elongase
mortarella
alpine
present
Prior art date
Application number
NO20082224A
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English (en)
Other versions
NO341310B1 (no
Inventor
Quinn Qun Zhu
Zhixiong Xue
Daniel Joseph Maccol
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of NO20082224L publication Critical patent/NO20082224L/no
Publication of NO341310B1 publication Critical patent/NO341310B1/no

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Sammendrag Den foreliggende oppfinnelsen vedrører en sopp C16/18 fettsyreelongase som er i stand til å katalysere omdanningen av palmitat (1 6:0) til stearinsyren (1 8:0). Spesifikk så blir nukleotidsekvensen til en Morteriella alpina C 16/I 8 fettsyreelongase tilveiebrakt (kalt 'TL03"). Fremgangsmåter for å øke mikrobiell oljeproduksjon, og øke karbonstrømming inn i den polyumettede fettsyrebiosyntetiske reaksjonsveien og å øke innholdet av polyumettede fettsyrer ved overuttrykk av C16/18 fettsyreelongasen er beskrevet her. Mest ønskelig så vil substratspesifisiteten til det foreliggende EL03 være spesielt nyttig for å muliggjøre akkumulering av langkjede polyumettede fettsyrer i oljeaktig gjær slik som Yarrowia lipolytika.
NO20082224A 2005-10-19 2008-05-14 En mortierella alpina C16/18 fettsyreelongase NO341310B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/253,882 US7470532B2 (en) 2005-10-19 2005-10-19 Mortierella alpina C16/18 fatty acid elongase
PCT/US2005/040141 WO2007046817A1 (en) 2005-10-19 2005-11-04 A mortierella alpina c16/18 fatty acid elongase

Publications (2)

Publication Number Publication Date
NO20082224L true NO20082224L (no) 2008-07-18
NO341310B1 NO341310B1 (no) 2017-10-02

Family

ID=37948592

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20082224A NO341310B1 (no) 2005-10-19 2008-05-14 En mortierella alpina C16/18 fettsyreelongase

Country Status (10)

Country Link
US (1) US7470532B2 (no)
EP (1) EP1937704B1 (no)
JP (1) JP4786715B2 (no)
KR (1) KR20080068074A (no)
CN (1) CN101563356B (no)
AU (1) AU2005337476B2 (no)
CA (1) CA2622398C (no)
DK (1) DK1937704T3 (no)
NO (1) NO341310B1 (no)
WO (1) WO2007046817A1 (no)

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EP2231864B1 (en) 2007-12-11 2017-05-24 Synthetic Genomics, Inc. Secretion of fatty acids by photosynthetic microorganisms
EP2358862B1 (en) 2008-12-18 2017-08-02 E. I. du Pont de Nemours and Company Reducing byproduction of malonates in a fermentation process
DE102009007402A1 (de) 2009-02-04 2010-08-05 VLB Berlin e. V. Institut für Mikrobiologie Biosynthese und Gewinnung von Substanzen aus Zellen
DK2442666T3 (da) 2009-06-16 2015-07-13 Du Pont Olier med højt indhold af eicosapentaensyre fra forbedrede og optimerede stammer af yarrowia lipolytica
JP5893555B2 (ja) 2009-06-16 2016-03-23 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ エイコサペンタエン酸を高産生するよう改良されたヤロウイア・リポリティカ（Ｙａｒｒｏｗｉａ ｌｉｐｏｌｙｔｉｃａ）最適化株
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US8956834B2 (en) 2009-06-29 2015-02-17 Synthetic Genomics, Inc. Acyl-ACP thioesterase genes and uses therefor
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US20120204802A1 (en) 2010-08-11 2012-08-16 E. I. Du Pont De Nemours And Company Sustainable aquaculture feeding strategy
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
JP2013535987A (ja) 2010-08-26 2013-09-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 高レベルのエイコサペンタエン酸生産のための組換え微生物宿主細胞
WO2012027676A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
BR112013004355A2 (pt) 2010-08-26 2021-05-04 E.I. Du Pont De Nemours And Company polipeptídeo mutante possuindo atividade delta-5 dessaturase, molécula de ácido nucleico isolada e célula transformada.
WO2012091812A1 (en) 2010-12-30 2012-07-05 E. I. Du Pont De Nemours And Company Use of saccharomyces cerevisiae suc2 gene in yarrowia lipolytica for sucrose utilization
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
US8969049B2 (en) 2011-03-31 2015-03-03 E I Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
EP2694665A1 (en) 2011-04-01 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
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AU2018241920B2 (en) * 2017-03-29 2022-04-21 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell with altered membrane lipid composition
CN110358692B (zh) * 2018-04-09 2021-07-27 中国科学院青岛生物能源与过程研究所 生产神经酸的重组酵母菌株及其应用
CN111020018B (zh) * 2019-11-28 2021-09-14 天津金匙医学科技有限公司 一种基于宏基因组学的病原微生物检测方法及试剂盒
CN112592926A (zh) * 2020-12-28 2021-04-02 江南大学 一种crispr系统及其在高山被孢霉中的应用
CN112661821B (zh) * 2021-01-21 2022-04-12 江南大学 一种柠檬酸转运蛋白及其在脂质合成中的应用

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Also Published As

Publication number Publication date
WO2007046817A1 (en) 2007-04-26
KR20080068074A (ko) 2008-07-22
AU2005337476A1 (en) 2007-04-26
EP1937704B1 (en) 2014-12-17
EP1937704A1 (en) 2008-07-02
CN101563356A (zh) 2009-10-21
JP4786715B2 (ja) 2011-10-05
CA2622398A1 (en) 2007-04-26
CN101563356B (zh) 2012-12-12
NO341310B1 (no) 2017-10-02
JP2009512437A (ja) 2009-03-26
US20070087420A1 (en) 2007-04-19
US7470532B2 (en) 2008-12-30
CA2622398C (en) 2014-01-21
DK1937704T3 (en) 2015-03-23
AU2005337476B2 (en) 2011-07-21
EP1937704A4 (en) 2009-12-16

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