NO20070946L - Genetisk fremstilt pyrroloquinolinquinonavhengig glukosedehydrogenase som omfatter et aminosyreinnskudd. - Google Patents

Genetisk fremstilt pyrroloquinolinquinonavhengig glukosedehydrogenase som omfatter et aminosyreinnskudd.

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Publication number
NO20070946L
NO20070946L NO20070946A NO20070946A NO20070946L NO 20070946 L NO20070946 L NO 20070946L NO 20070946 A NO20070946 A NO 20070946A NO 20070946 A NO20070946 A NO 20070946A NO 20070946 L NO20070946 L NO 20070946L
Authority
NO
Norway
Prior art keywords
amino acid
pyrroloquinoline quinone
dependent glucose
glucose dehydrogenase
acid insert
Prior art date
Application number
NO20070946A
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English (en)
Other versions
NO340499B1 (no
Inventor
Mara Boenitz Dulat
Wolfgang-Reinhold Knappe
Peter Kratzsch
Jessica Laggerbauer
Rainer Schmuck
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of NO20070946L publication Critical patent/NO20070946L/no
Publication of NO340499B1 publication Critical patent/NO340499B1/no

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/40—Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas

Abstract

Foreliggende oppfinnelse vedrører forbedrede varianter av løselige pyrrolokinolinkinon (PQQ)-avhengige glukosedehydrogenaser (s-GDH) som omfatter et aminosyreinnskudd mellom posisjonene 428 og 429 tilsvarende aminosyresekvensen som er kjent fra Acinetobacter calcoaceticus, til gener som koder for slike variant-s-GDH, til proteiner av slike s-GDH-varianter med forbedret substratspesifisitet for glukose og fil ulike applikasjoner av disse s-GDH-variantene, spesielt for å bestemme konsentrasjoner av sukker, spesielt av glukose i en prøve.
NO20070946A 2004-07-20 2007-02-19 Variant av den løselige formen av pyrrolokinolinkinonavhengig glukosedehydrogenase som omfatter én aminosyre insersjon og minst én aminosyre substitusjon, et isolert polynukleotid som koder for varianten, en ekspresjonsvektor som omfatter det isolert polynukleotidet, en vertscelle som omfatter ekspresjonsvektoren, en fremgangsmåte for fremstilling av s-GDH-varianter som omfatter dyrking av vertscellen, en fremgangsmåte for fremstilling av s-GDH-varianter i et cellefritt peptidsyntesesystem, en fremgangsmåte for påvisning, bestemmelse eller måling av glukose i en prøve ved å anvende en variant, samt en innretning for påvisningen eller målingen av glukose i en prøve. NO340499B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04017069 2004-07-20
PCT/EP2005/007844 WO2006008132A1 (en) 2004-07-20 2005-07-19 Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion

Publications (2)

Publication Number Publication Date
NO20070946L true NO20070946L (no) 2007-04-11
NO340499B1 NO340499B1 (no) 2017-05-02

Family

ID=34925823

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20070946A NO340499B1 (no) 2004-07-20 2007-02-19 Variant av den løselige formen av pyrrolokinolinkinonavhengig glukosedehydrogenase som omfatter én aminosyre insersjon og minst én aminosyre substitusjon, et isolert polynukleotid som koder for varianten, en ekspresjonsvektor som omfatter det isolert polynukleotidet, en vertscelle som omfatter ekspresjonsvektoren, en fremgangsmåte for fremstilling av s-GDH-varianter som omfatter dyrking av vertscellen, en fremgangsmåte for fremstilling av s-GDH-varianter i et cellefritt peptidsyntesesystem, en fremgangsmåte for påvisning, bestemmelse eller måling av glukose i en prøve ved å anvende en variant, samt en innretning for påvisningen eller målingen av glukose i en prøve.

Country Status (16)

Country Link
US (1) US7547524B2 (no)
EP (2) EP3521421A1 (no)
JP (1) JP4842938B2 (no)
KR (1) KR100879491B1 (no)
CN (1) CN100549167C (no)
AU (1) AU2005263591B2 (no)
BR (1) BRPI0513691A (no)
CA (1) CA2570593C (no)
HK (1) HK1107829A1 (no)
IL (1) IL179750A (no)
MX (1) MX2007000560A (no)
NO (1) NO340499B1 (no)
NZ (1) NZ551870A (no)
RU (1) RU2336306C1 (no)
WO (1) WO2006008132A1 (no)
ZA (1) ZA200700544B (no)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2010649T (pt) 2006-04-13 2017-05-03 Hoffmann La Roche Mutantes melhorados da glicose-desidrogenase solúvel dependente de pirroloquinolina quinona
US8900431B2 (en) 2008-08-27 2014-12-02 Edwards Lifesciences Corporation Analyte sensor
FR2948680B1 (fr) 2009-07-28 2013-10-04 Centre Nat Rech Scient Nouveaux mutants de la pqq s-gdh
WO2011050340A1 (en) * 2009-10-23 2011-04-28 Life Technologies Corporation Systems and methods for error correction in dna sequencing
US20130092536A1 (en) 2010-07-23 2013-04-18 Roche Diagnostics Operations, Inc. Zwitterion buffer containing compositions and uses in electroanalytical methods
EP2465936A1 (en) 2010-12-20 2012-06-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of statins and intermediates thereof
JP6194251B2 (ja) 2010-12-20 2017-09-06 レツク・フアーマシユーテイカルズ・デー・デー 医薬品有効成分およびこれの中間体の酵素的合成
EP2656058A1 (en) 2010-12-22 2013-10-30 Roche Diagnostics GmbH Systems and methods to compensate for sources of error during electrochemical testing
CN105331591B (zh) 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 PQQ-sGDH突变体、聚核苷酸及葡萄糖检测装置

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3716957A1 (de) 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
DE4311464A1 (de) 1993-04-08 1994-10-13 Boehringer Mannheim Gmbh Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase
DE19639169A1 (de) * 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
JPH10243786A (ja) 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
US5997817A (en) 1997-12-05 1999-12-07 Roche Diagnostics Corporation Electrochemical biosensor test strip
JPH11243949A (ja) 1998-03-03 1999-09-14 Toyobo Co Ltd Ｐｑｑを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法
JP2000350588A (ja) 1999-04-08 2000-12-19 Koji Hayade グルコース脱水素酵素
TWI224136B (en) * 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
CA2427029C (en) 2000-10-27 2010-02-02 F. Hoffmann-La Roche Ag Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
US6507120B2 (en) * 2000-12-22 2003-01-14 Siliconware Precision Industries Co., Ltd. Flip chip type quad flat non-leaded package
US7476525B2 (en) * 2002-05-27 2009-01-13 Toyo Boseki Kabushiki Kaisha Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability
JP2004173538A (ja) 2002-11-25 2004-06-24 Amano Enzyme Inc ピロロキノリンキノン依存性グルコース脱水素酵素

Also Published As

Publication number Publication date
CA2570593C (en) 2012-03-20
IL179750A0 (en) 2007-05-15
US20070243566A1 (en) 2007-10-18
CN100549167C (zh) 2009-10-14
BRPI0513691A (pt) 2008-03-25
KR100879491B1 (ko) 2009-01-20
RU2336306C1 (ru) 2008-10-20
NO340499B1 (no) 2017-05-02
WO2006008132A1 (en) 2006-01-26
CA2570593A1 (en) 2006-01-26
EP1771553A1 (en) 2007-04-11
JP2008506375A (ja) 2008-03-06
JP4842938B2 (ja) 2011-12-21
KR20070032794A (ko) 2007-03-22
HK1107829A1 (en) 2008-04-18
AU2005263591B2 (en) 2008-08-14
IL179750A (en) 2015-11-30
CN1989241A (zh) 2007-06-27
AU2005263591A1 (en) 2006-01-26
US7547524B2 (en) 2009-06-16
EP3521421A1 (en) 2019-08-07
ZA200700544B (en) 2008-10-29
NZ551870A (en) 2009-02-28
MX2007000560A (es) 2007-03-07

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