NL8801601A - Een "swine-kidney" celcultuur, geschikt voor het kweken van virussen, een daaraan geadapteerde "chinese" stam van het varkenspestvirus, alsmede de hiervan afgeleide vaccins. - Google Patents
Een "swine-kidney" celcultuur, geschikt voor het kweken van virussen, een daaraan geadapteerde "chinese" stam van het varkenspestvirus, alsmede de hiervan afgeleide vaccins. Download PDFInfo
- Publication number
- NL8801601A NL8801601A NL8801601A NL8801601A NL8801601A NL 8801601 A NL8801601 A NL 8801601A NL 8801601 A NL8801601 A NL 8801601A NL 8801601 A NL8801601 A NL 8801601A NL 8801601 A NL8801601 A NL 8801601A
- Authority
- NL
- Netherlands
- Prior art keywords
- virus
- cells
- swine
- chinese
- kidney
- Prior art date
Links
- 241000700605 Viruses Species 0.000 title claims abstract description 99
- 229960005486 vaccine Drugs 0.000 title claims abstract description 21
- 238000004113 cell culture Methods 0.000 title description 7
- 241000710777 Classical swine fever virus Species 0.000 claims abstract description 18
- 238000004114 suspension culture Methods 0.000 claims abstract description 8
- 210000004027 cell Anatomy 0.000 claims description 81
- 238000012258 culturing Methods 0.000 claims description 3
- 210000004962 mammalian cell Anatomy 0.000 claims description 3
- 238000000034 method Methods 0.000 claims 3
- 241000282898 Sus scrofa Species 0.000 abstract description 19
- 210000003734 kidney Anatomy 0.000 abstract description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 15
- 239000000725 suspension Substances 0.000 description 11
- 241000282887 Suidae Species 0.000 description 10
- 206010037660 Pyrexia Diseases 0.000 description 8
- 230000006978 adaptation Effects 0.000 description 8
- 230000012010 growth Effects 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 6
- 238000011109 contamination Methods 0.000 description 5
- 239000002356 single layer Substances 0.000 description 5
- 241000283690 Bos taurus Species 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 206010012735 Diarrhoea Diseases 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 210000003292 kidney cell Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000004264 monolayer culture Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 239000006145 Eagle's minimal essential medium Substances 0.000 description 2
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- 241000702619 Porcine parvovirus Species 0.000 description 2
- RADKZDMFGJYCBB-UHFFFAOYSA-N Pyridoxal Chemical compound CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000701093 Suid alphaherpesvirus 1 Species 0.000 description 2
- 241000711484 Transmissible gastroenteritis virus Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000007918 intramuscular administration Methods 0.000 description 2
- 231100000636 lethal dose Toxicity 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 238000003307 slaughter Methods 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- QZNNVYOVQUKYSC-JEDNCBNOSA-N (2s)-2-amino-3-(1h-imidazol-5-yl)propanoic acid;hydron;chloride Chemical compound Cl.OC(=O)[C@@H](N)CC1=CN=CN1 QZNNVYOVQUKYSC-JEDNCBNOSA-N 0.000 description 1
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- QDGAVODICPCDMU-UHFFFAOYSA-N 2-amino-3-[3-[bis(2-chloroethyl)amino]phenyl]propanoic acid Chemical compound OC(=O)C(N)CC1=CC=CC(N(CCCl)CCCl)=C1 QDGAVODICPCDMU-UHFFFAOYSA-N 0.000 description 1
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 description 1
- VVIAGPKUTFNRDU-UHFFFAOYSA-N 6S-folinic acid Natural products C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 VVIAGPKUTFNRDU-UHFFFAOYSA-N 0.000 description 1
- 241000701386 African swine fever virus Species 0.000 description 1
- KWTQSFXGGICVPE-WCCKRBBISA-N Arginine hydrochloride Chemical compound Cl.OC(=O)[C@@H](N)CCCN=C(N)N KWTQSFXGGICVPE-WCCKRBBISA-N 0.000 description 1
- 206010008631 Cholera Diseases 0.000 description 1
- 235000019743 Choline chloride Nutrition 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 241000709661 Enterovirus Species 0.000 description 1
- MPJKWIXIYCLVCU-UHFFFAOYSA-N Folinic acid Natural products NC1=NC2=C(N(C=O)C(CNc3ccc(cc3)C(=O)NC(CCC(=O)O)CC(=O)O)CN2)C(=O)N1 MPJKWIXIYCLVCU-UHFFFAOYSA-N 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 1
- 235000013878 L-cysteine Nutrition 0.000 description 1
- 239000004201 L-cysteine Substances 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 229930182816 L-glutamine Natural products 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- 229930182844 L-isoleucine Natural products 0.000 description 1
- 235000019454 L-leucine Nutrition 0.000 description 1
- 239000004395 L-leucine Substances 0.000 description 1
- BVHLGVCQOALMSV-JEDNCBNOSA-N L-lysine hydrochloride Chemical compound Cl.NCCCC[C@H](N)C(O)=O BVHLGVCQOALMSV-JEDNCBNOSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 229930195722 L-methionine Natural products 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 208000037581 Persistent Infection Diseases 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 206010051511 Viral diarrhoea Diseases 0.000 description 1
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 229960003178 choline chloride Drugs 0.000 description 1
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000003246 corticosteroid Substances 0.000 description 1
- 229960001334 corticosteroids Drugs 0.000 description 1
- 239000012297 crystallization seed Substances 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- JROGBPMEKVAPEH-GXGBFOEMSA-N emetine dihydrochloride Chemical compound Cl.Cl.N1CCC2=CC(OC)=C(OC)C=C2[C@H]1C[C@H]1C[C@H]2C3=CC(OC)=C(OC)C=C3CCN2C[C@@H]1CC JROGBPMEKVAPEH-GXGBFOEMSA-N 0.000 description 1
- VVIAGPKUTFNRDU-ABLWVSNPSA-N folinic acid Chemical compound C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 VVIAGPKUTFNRDU-ABLWVSNPSA-N 0.000 description 1
- 235000008191 folinic acid Nutrition 0.000 description 1
- 239000011672 folinic acid Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- PGBHMTALBVVCIT-VCIWKGPPSA-N framycetin Chemical compound N[C@@H]1[C@@H](O)[C@H](O)[C@H](CN)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](N)C[C@@H](N)[C@@H]2O)O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CN)O2)N)O[C@@H]1CO PGBHMTALBVVCIT-VCIWKGPPSA-N 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229960003136 leucine Drugs 0.000 description 1
- 229960001691 leucovorin Drugs 0.000 description 1
- 201000002364 leukopenia Diseases 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 229960004452 methionine Drugs 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229940053050 neomycin sulfate Drugs 0.000 description 1
- 229940014662 pantothenate Drugs 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229960003581 pyridoxal Drugs 0.000 description 1
- 235000008164 pyridoxal Nutrition 0.000 description 1
- 239000011674 pyridoxal Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 229960002898 threonine Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 229960004441 tyrosine Drugs 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/187—Hog cholera virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
- A61K2039/552—Veterinary vaccine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24061—Methods of inactivation or attenuation
- C12N2770/24064—Methods of inactivation or attenuation by serial passage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Public Health (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Epidemiology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL8801601A NL8801601A (nl) | 1988-06-23 | 1988-06-23 | Een "swine-kidney" celcultuur, geschikt voor het kweken van virussen, een daaraan geadapteerde "chinese" stam van het varkenspestvirus, alsmede de hiervan afgeleide vaccins. |
| EP89201574A EP0351901B1 (en) | 1988-06-23 | 1989-06-15 | Swine kidney cell culture, and its use in vaccine production |
| ES89201574T ES2052883T3 (es) | 1988-06-23 | 1989-06-15 | Cultivo de celulas de riñon de cerdo y su uso en la produccion de vacunas. |
| AT89201574T ATE83929T1 (de) | 1988-06-23 | 1989-06-15 | Schweinenierenzellkultur und ihre verwendung fuer die herstellung von vakzinen. |
| DE8989201574T DE68904131T2 (de) | 1988-06-23 | 1989-06-15 | Schweinenierenzellkultur und ihre verwendung fuer die herstellung von vakzinen. |
| GR930400220T GR3006970T3 (cs) | 1988-06-23 | 1993-02-04 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL8801601A NL8801601A (nl) | 1988-06-23 | 1988-06-23 | Een "swine-kidney" celcultuur, geschikt voor het kweken van virussen, een daaraan geadapteerde "chinese" stam van het varkenspestvirus, alsmede de hiervan afgeleide vaccins. |
| NL8801601 | 1988-06-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL8801601A true NL8801601A (nl) | 1990-01-16 |
Family
ID=19852512
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL8801601A NL8801601A (nl) | 1988-06-23 | 1988-06-23 | Een "swine-kidney" celcultuur, geschikt voor het kweken van virussen, een daaraan geadapteerde "chinese" stam van het varkenspestvirus, alsmede de hiervan afgeleide vaccins. |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0351901B1 (cs) |
| AT (1) | ATE83929T1 (cs) |
| DE (1) | DE68904131T2 (cs) |
| ES (1) | ES2052883T3 (cs) |
| GR (1) | GR3006970T3 (cs) |
| NL (1) | NL8801601A (cs) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CZ295138B6 (cs) * | 1994-06-17 | 2005-05-18 | Instituut Voor Dierhouderij En Diergezondheid | Nukleotidové sekvence pestivirových kmenů, polypeptidy kódované těmito sekvencemi a jejich použití v diagnostice a prevenci pestivirových infekcí |
| RU2180237C1 (ru) * | 2001-03-02 | 2002-03-10 | Федеральное государственное учреждение Всероссийский научно-исследовательский институт защиты животных | Штамм "синлак" вируса pestis suum для изготовления вакцинных препаратов |
| EP2280069A1 (en) | 2006-09-20 | 2011-02-02 | Institut Pasteur Korea | A method of detecting and/or quantifying expression of a target protein candidate in a cell, and a method of identifying a target protein of a small molecule modulator |
| CN103336116B (zh) * | 2010-06-22 | 2016-01-13 | 普莱柯生物工程股份有限公司 | 一种猪瘟活疫苗效力检验方法 |
| US20120148559A1 (en) | 2010-12-01 | 2012-06-14 | Board Of Regents The University Of Texas System | Compositions and method for deimmunization of proteins |
| CN104651378B (zh) * | 2015-02-02 | 2017-10-24 | 武汉大学 | 一种猪瘟疫苗感染性cDNA及其构建方法和应用 |
| CN111454910A (zh) * | 2020-03-13 | 2020-07-28 | 成都天邦生物制品有限公司 | 表达猪cd163和cd169分子的悬浮pk15细胞系 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3422188A (en) * | 1965-08-17 | 1969-01-14 | American Cyanamid Co | Tissue culture hog cholera vaccine |
| FR2088038B1 (cs) * | 1970-05-11 | 1973-08-10 | Bellon Labor Sa Roger | |
| FR2244542A1 (en) * | 1973-08-08 | 1975-04-18 | Bellon Labor Sa Roger | Vaccines prepd from IB-RS-2- cells - for treatment of porcine pests |
-
1988
- 1988-06-23 NL NL8801601A patent/NL8801601A/nl not_active Application Discontinuation
-
1989
- 1989-06-15 DE DE8989201574T patent/DE68904131T2/de not_active Expired - Fee Related
- 1989-06-15 EP EP89201574A patent/EP0351901B1/en not_active Expired - Lifetime
- 1989-06-15 AT AT89201574T patent/ATE83929T1/de not_active IP Right Cessation
- 1989-06-15 ES ES89201574T patent/ES2052883T3/es not_active Expired - Lifetime
-
1993
- 1993-02-04 GR GR930400220T patent/GR3006970T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| GR3006970T3 (cs) | 1993-06-30 |
| EP0351901A1 (en) | 1990-01-24 |
| ES2052883T3 (es) | 1994-07-16 |
| ATE83929T1 (de) | 1993-01-15 |
| EP0351901B1 (en) | 1992-12-30 |
| DE68904131D1 (de) | 1993-02-11 |
| DE68904131T2 (de) | 1993-07-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sikes et al. | Effective protection of monkeys against death from street virus by post-exposure administration of tissue-culture rabies vaccine | |
| JPH01230532A (ja) | 新規なワクチン及びその製法 | |
| AU5103293A (en) | Immortalized cells and uses therefor | |
| NL9401414A (nl) | Vaccin ter voorkoming van varkensreproductie- en -respiratiesyndroom. | |
| Osuagwuh et al. | Absence of lumpy skin disease virus in semen of vaccinated bulls following vaccination and subsequent experimental infection | |
| Dorson et al. | Infectious pancreatic necrosis virus of salmonids: biological and antigenic features of a pathogenic strain and of a non‐pathogenic variant selected in RTG‐2 cells | |
| EP1129723B1 (en) | Combined vaccine against both hav and measle virus and method of producing it | |
| NO753676L (cs) | ||
| KR101617464B1 (ko) | Ｉｐｖ―ｄｐｔ 백신 | |
| EP0687471A1 (en) | Production of an efficacious toxoplasma gondii bradyzoite vaccine in tissue culture | |
| NL8801601A (nl) | Een "swine-kidney" celcultuur, geschikt voor het kweken van virussen, een daaraan geadapteerde "chinese" stam van het varkenspestvirus, alsmede de hiervan afgeleide vaccins. | |
| US3642574A (en) | Method for producing vaccine for immunization of poultry against marek{3 s disease | |
| CN102727884A (zh) | 猪繁殖与呼吸综合征、伪狂犬病二联活疫苗及其制备方法 | |
| RU2403063C1 (ru) | Вакцина инактивированная комбинированная против вирусной диареи, рота-, коронавирусной болезней и эшерихиоза крупного рогатого скота | |
| Bell | An introduction to general virology | |
| Lawson et al. | Safety and immunogenicity of ERA strain of rabies virus propagated in a BHK-21 cell line | |
| CN102727883A (zh) | 猪繁殖与呼吸综合征与猪瘟二联疫苗及其用途 | |
| Yoshida et al. | Establishment of an attenuated ML-17 strain of Japanese encephalitis virus. | |
| US4229434A (en) | Vaccine for prophylaxis of trichophytosis in horse and method of preparing same | |
| JPS60248179A (ja) | 家禽アデノウイルス２型の増殖方法 | |
| US4312947A (en) | Process for the preparation of a vaccine against panleucopenia of the cat | |
| RU2127602C1 (ru) | Штамм "бг" вируса инфекционной бурсальной болезни птиц и вакцина против инфекционной бурсальной болезни птиц | |
| Roy | Fish rhabdoviruses | |
| Taylor et al. | History of vaccines and vaccination | |
| Tzipori | The isolation of bovine ephemeral fever virus in cell cultures and evidence for autointerference |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A1B | A search report has been drawn up | ||
| BV | The patent application has lapsed |