NL2033320B1 - Method for detecting cmpf content in plasma by high performance liquid chromatography tandem mass spectrometry - Google Patents
Method for detecting cmpf content in plasma by high performance liquid chromatography tandem mass spectrometry Download PDFInfo
- Publication number
- NL2033320B1 NL2033320B1 NL2033320A NL2033320A NL2033320B1 NL 2033320 B1 NL2033320 B1 NL 2033320B1 NL 2033320 A NL2033320 A NL 2033320A NL 2033320 A NL2033320 A NL 2033320A NL 2033320 B1 NL2033320 B1 NL 2033320B1
- Authority
- NL
- Netherlands
- Prior art keywords
- cmpf
- plasma
- mass spectrometry
- liquid chromatography
- performance liquid
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000004128 high performance liquid chromatography Methods 0.000 title claims abstract description 23
- 238000004885 tandem mass spectrometry Methods 0.000 title claims abstract description 17
- WMCQWXZMVIETAO-UHFFFAOYSA-N 2-(2-carboxyethyl)-4-methyl-5-propylfuran-3-carboxylic acid Chemical compound CCCC=1OC(CCC(O)=O)=C(C(O)=O)C=1C WMCQWXZMVIETAO-UHFFFAOYSA-N 0.000 claims abstract description 84
- 238000001514 detection method Methods 0.000 claims abstract description 19
- 238000002203 pretreatment Methods 0.000 claims abstract description 19
- 150000001875 compounds Chemical class 0.000 claims abstract description 16
- 238000004949 mass spectrometry Methods 0.000 claims abstract description 8
- 238000011002 quantification Methods 0.000 claims abstract description 8
- 238000011088 calibration curve Methods 0.000 claims abstract description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 36
- 239000000126 substance Substances 0.000 claims description 23
- 239000003960 organic solvent Substances 0.000 claims description 14
- 150000002500 ions Chemical class 0.000 claims description 13
- 238000002552 multiple reaction monitoring Methods 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 238000004807 desolvation Methods 0.000 claims description 8
- 230000006920 protein precipitation Effects 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 7
- 238000010828 elution Methods 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
- 239000011550 stock solution Substances 0.000 claims description 6
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- IWXRPVHRDWXIEA-UHFFFAOYSA-N azanium formate hydrate Chemical compound [NH4+].O.[O-]C=O IWXRPVHRDWXIEA-UHFFFAOYSA-N 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
- 238000001819 mass spectrum Methods 0.000 claims description 3
- 238000004704 ultra performance liquid chromatography Methods 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims description 3
- 102000007562 Serum Albumin Human genes 0.000 claims description 2
- 108010071390 Serum Albumin Proteins 0.000 claims description 2
- 244000309464 bull Species 0.000 claims description 2
- 238000004811 liquid chromatography Methods 0.000 claims 1
- 238000010813 internal standard method Methods 0.000 abstract description 3
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 44
- 238000003018 immunoassay Methods 0.000 description 6
- 239000011159 matrix material Substances 0.000 description 6
- 238000010811 Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Methods 0.000 description 5
- 208000002705 Glucose Intolerance Diseases 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 4
- 241001669573 Galeorhinus galeus Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 210000003719 b-lymphocyte Anatomy 0.000 description 3
- 206010012601 diabetes mellitus Diseases 0.000 description 3
- 208000004104 gestational diabetes Diseases 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 201000009104 prediabetes syndrome Diseases 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical compound C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000003914 insulin secretion Effects 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 206010018429 Glucose tolerance impaired Diseases 0.000 description 1
- 101001091385 Homo sapiens Kallikrein-6 Proteins 0.000 description 1
- 102100034866 Kallikrein-6 Human genes 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004129 fatty acid metabolism Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002441 uremic toxin Substances 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
- G01N2030/045—Standards internal
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Claims (3)
1. Werkwijze voor het detecteren van het gehalte aan 3-carboxy-4- methyl-5-propyl-2-furanpropaanzuur (CMPF) in plasma door middel van tandemmassaspectrometrie met hoogwaardige vloeistofchromato- grafie, met het kenmerk, dat de werkwijze de volgende stappen om- vat: voorbehandeling van plasmamonster: nauwkeurig pipetteren van 100 uL van een plasmamonster en plaatsen op een 96-wells eiwitprecipi- tatieplaat, toevoegen van een organisch oplosmiddel met een inter- ne standaard van CMPF-isotoop, plaatsen in een oscillator met con- stante temperatuur, schudden bij 700 rpm gedurende 3 min, plaatsen op een apparaat met positieve druk, aanpassen van een stroomsnel- heid tot 40 psi, het nemen van gefilterde oplossing en het gebrui- ken als een detectiemonster voor tandemmassaspectrometrie voor vloeistofchromatografie voor stand-by; en detectie van het CMPF-gehalte in het voorbehandelde plasmamonster met behulp van de tandemmassaspectrometrie met hoogwaardige vloei- stofchromatografie: eerst met behulp van hoogwaardige vloeistof- chromatografie om CMPF te scheiden, en vervolgens met behulp van een interne standaardmethode voor massaspectrometrie voor kwanti- ficering, met behulp van de concentratie verhouding van een CMPF- doelverbinding en een interne CMPF-isotoopstandaard als X-as, en met behulp van de piekoppervlakverhouding van de CMPF- doelverbinding en de interne CMPF-isotoopstandaard als Y-as, om een kalibratiecurve vast te stellen, en het berekenen van het CMPF-gehalte in het plasma, waarbij de specifieke instrument- omstandigheden als volgt zijn: (1) omstandigheden voor hoogwaardige vloeistofchromatografie: mobiele fase A: 5 mM ammoniumformiaat water; mobiele fase B: 0,1% mierenzuur + methanol; chromatografisch kolommodel: Waters UPLC HSS C18SB 2,1 mmx50 mm, 1,8 um de kolomtemperatuur is 40 °C + 5 °C, het injectievolume is 10 pL, de temperatuur van een monsterinjectielade is 10 °C + 5 °C, en de stroomsnelheid is 0,4 ml / min; en er wordt een gradiëntelutiemodus gebruikt, zoals weergegeven in tabel 1 hieronder: Tabel 1: Gradiënt elutie procedure Tijd (min) Stroomsnelheid (mL/min) A% B% Curve 00 0400 800 200 6
0.5 0.400 80.0 200 6
0.8 0.400 950 50 6 15 0.400 950 50 6 1,51 0.400 80.0 200 6
2.0 0.400 800 200 6 (2) massaspectrometrie omstandigheden: ionisatiemodus: in een elektrospray-positieve ionen modus wordt een massaspectrumscanmodus van meervoudige reactiebewaking (MRM) gebruikt, de capillaire spanning is 4,0 kV; de temperatuur van de ionenbron is 150 °C; de desolvatie gastemperatuur is 650 °C; het desolvatiegasdebiet is 650 l/u; en de doelverbinding en de interne standaard van de isotoop worden gelijktijdig ge- volgd; en MRM-modus: ionparameter: CMPF: 239.1596>151.114, de kegelspan- ning is 32 V, en de botsingsenergie is 16 V; en CMPF-d3:
242.1596>154.173, de kegelspanning is 32 V en de botsingsenergie is 18 V.
2. Werkwijze voor het detecteren van het CMPF-gehalte in het plas- ma door middel van tandemmassaspectrometrie met hoogwaardige vloeistofchromatografie volgens conclusie 1, met het kenmerk, dat bij de voorbehandeling van het plasmamonster het toegevoegde orga- nische oplosmiddel dat de interne standaard van het CMPF-isotoop bevat, wordt bereid volgens de volgende werkwijze: gebruik de me- thanol als oplosmiddel om 1 mg/ml CMPF-d3 standaardstof- voorraadoplossing te bereiden en gebruik vervolgens de methanol om het organische oplosmiddel te bereiden dat 1,0 pg/ml CMPF-d3 be-
vat.
3. Werkwijze voor het detecteren van het CMPF-gehalte in het plas- ma door middel van tandemmassaspectrometrie met hoogwaardige vloeistofchromatografie volgens conclusie 1, met het kenmerk, dat de CMPF-doelverbinding wordt bereid door de volgende werkwijze: met gebruikmaking van de methanol als oplosmiddel om 1 mg/ml CMPF- standaardstofvoorraadoplossing te bereiden, en vervolgens gebruik maken van 5% m/v stierserumalbumine (BSA) -oplossing om in totaal 7 gradiënten van gemengde standaardstofoplossing te bereiden met CMPF-concentraties van 0,02 pg/ml, 0,1 ug/ml , 0,5 pg/ml, 1,0 pg/mL, 5,0 pg/ml, 10,0 pg/mL, en 50 pg/ml en het afzonderlijk in een bruine fles van 1,5 ml brengen en bij -20 °C bewaren voor la- ter gebruik.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210779049.6A CN114924011B (zh) | 2022-06-30 | 2022-06-30 | 高效液相色谱串联质谱法检测血浆中cmpf含量的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL2033320B1 true NL2033320B1 (en) | 2024-01-18 |
Family
ID=82816167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL2033320A NL2033320B1 (en) | 2022-06-30 | 2022-10-14 | Method for detecting cmpf content in plasma by high performance liquid chromatography tandem mass spectrometry |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN114924011B (nl) |
| NL (1) | NL2033320B1 (nl) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104822378A (zh) * | 2012-09-21 | 2015-08-05 | 多伦多大学理事会 | 作为糖尿病的生物标志物的cmpf和相关方法 |
| CN109884317B (zh) * | 2019-03-12 | 2022-03-08 | 杭州博谱医药科技有限公司 | 氧化型硫代辅酶i在均相酶免疫诊断试剂中的应用 |
| CN110907569A (zh) * | 2019-12-25 | 2020-03-24 | 山东英盛生物技术有限公司 | 一种同时检测血液样品中4种蛋白结合尿毒症毒素的方法 |
| CN112326829A (zh) * | 2020-11-03 | 2021-02-05 | 浙江大学滨海产业技术研究院 | 一种基于代谢组学通路的慢性肾病早期预警方法 |
| CN114152743A (zh) * | 2021-12-02 | 2022-03-08 | 沈阳易康元血液透析科技有限公司 | 一种检测cmpf的化学发光免疫检测试剂盒及其检测方法 |
-
2022
- 2022-06-30 CN CN202210779049.6A patent/CN114924011B/zh active Active
- 2022-10-14 NL NL2033320A patent/NL2033320B1/en active
Non-Patent Citations (4)
| Title |
|---|
| MARIA A. LANKINEN ET AL: "CMPF Does Not Associate with Impaired Glucose Metabolism in Individuals with Features of Metabolic Syndrome", PLOS ONE, vol. 10, no. 4, 15 April 2015 (2015-04-15), pages e0124379, XP055438452, DOI: 10.1371/journal.pone.0124379 * |
| NIWA T ET AL: "Rapid assay for furancarboxylic acid accumulated in uremic serum using high-performance liquid chromatography and on-line mass spectrometry", CLINICA CHIMICA ACTA, ELSEVIER BV, AMSTERDAM, NL, vol. 226, no. 1, 1 April 1994 (1994-04-01), pages 89 - 94, XP024785526, ISSN: 0009-8981, [retrieved on 19940401], DOI: 10.1016/0009-8981(94)90106-6 * |
| SUZUKI YOSUKE ET AL: "Simultaneous quantification of coproporphyrin-I and 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid in human plasma using ultra-high performance liquid chromatography coupled to tandem mass spectrometry", JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, ELSEVIER B.V, AMSTERDAM, NL, vol. 184, 24 February 2020 (2020-02-24), XP086128274, ISSN: 0731-7085, [retrieved on 20200224], DOI: 10.1016/J.JPBA.2020.113202 * |
| YOSHIHARU ITOH ET AL: "Protein-bound uremic toxins in hemodialysis patients measured by liquid chromatography/tandem mass spectrometry and their effects on endothelial ROS production", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER, BERLIN, DE, vol. 403, no. 7, 25 March 2012 (2012-03-25), pages 1841 - 1850, XP035059651, ISSN: 1618-2650, DOI: 10.1007/S00216-012-5929-3 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114924011B (zh) | 2023-08-25 |
| CN114924011A (zh) | 2022-08-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Shen et al. | Quantitative metabolic network profiling of Escherichia coli: An overview of analytical methods for measurement of intracellular metabolites | |
| Wang et al. | Simultaneous determination of creatine phosphate, creatine and 12 nucleotides in rat heart by LC–MS/MS | |
| Dunn et al. | Sample preparation related to the intracellular metabolome of yeast: methods for quenching, extraction, and metabolite quantitation | |
| CN102980968A (zh) | 一种尿液中肌酐的液相色谱串联质谱测定方法 | |
| US20240085358A1 (en) | Method for detecting adsorption performance of microplastics for heavy metals using low-field nmr relaxation method | |
| NL2033320B1 (en) | Method for detecting cmpf content in plasma by high performance liquid chromatography tandem mass spectrometry | |
| CN112730641B (zh) | 一种n-甲基哌嗪的离子色谱测定法 | |
| Van Gulik et al. | The sampling and sample preparation problem in microbial metabolomics | |
| Cravey et al. | Current status of blood alcohol methods | |
| CN113219089A (zh) | 一种柱后衍生-液相色谱检测尿素的方法 | |
| CN106483230B (zh) | 一种尿液羟基多环芳烃的快速检测方法 | |
| Zhou et al. | A molecularly imprinted fiber array solid-phase microextraction strategy for simultaneous detection of multiple estrogens | |
| CN111474280A (zh) | 复方氨基酸注射液中痕量铝元素的检测方法 | |
| CN111487329A (zh) | 一种同时测定血液和玻璃体液中乙醇非氧化代谢物的方法 | |
| CN111579690A (zh) | 一种利用霉酚酸-d3作为内标物测定生物样本中霉酚酸含量的质谱检测试剂及其使用方法 | |
| CN112485340A (zh) | 一种超高效液相色谱串联质谱检测血浆中1,5-脱水山梨醇的方法 | |
| Zhen | Rapid determination of alcohol content in wine products using internal standard method of 1H nuclear magnetic resonance. | |
| CN113030352B (zh) | 一种厄贝沙坦中nmba含量的测定分析方法 | |
| CN108680661A (zh) | 一种新精神活性物质bzp的检测方法 | |
| CN111812243B (zh) | 一种消减全血环孢素测定的基质效应的分析方法 | |
| CN117805265A (zh) | 一种洛索洛芬钠及其制剂中遗传毒性杂质的分析方法 | |
| CN105651893A (zh) | 一种饲料添加剂中纽甜含量的高效液相色谱测定方法 | |
| CN115015365A (zh) | 一种快速检测尿液中尼古丁和可替宁的在线电离质谱方法 | |
| Wright et al. | Detection of endogenous and drug-modulated CYP3A activity in cells using LC-MS/MS | |
| CN116399982A (zh) | 一种检测血浆中头孢克洛浓度的生物分析方法 |