NL2030976A - Primer probe set and kit for rt-pcr detection of human leukotriene receptor cysltr1 mrna - Google Patents

Primer probe set and kit for rt-pcr detection of human leukotriene receptor cysltr1 mrna Download PDF

Info

Publication number
NL2030976A
NL2030976A NL2030976A NL2030976A NL2030976A NL 2030976 A NL2030976 A NL 2030976A NL 2030976 A NL2030976 A NL 2030976A NL 2030976 A NL2030976 A NL 2030976A NL 2030976 A NL2030976 A NL 2030976A
Authority
NL
Netherlands
Prior art keywords
probe
primer
cysltr1
kit
seq
Prior art date
Application number
NL2030976A
Other languages
English (en)
Other versions
NL2030976B1 (en
Inventor
Wu Shandong
Qian Lei
Liu Yi
Wang Jiaofeng
Lei Wei
Wu Zhoujie
Jiang Xuehan
Original Assignee
Hangzhou Zheda Dixun Biological Gene Eng Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hangzhou Zheda Dixun Biological Gene Eng Co Ltd filed Critical Hangzhou Zheda Dixun Biological Gene Eng Co Ltd
Publication of NL2030976A publication Critical patent/NL2030976A/en
Application granted granted Critical
Publication of NL2030976B1 publication Critical patent/NL2030976B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (10)

CONCLUSIES
1. Primer probe set voor detectie van reverse transcriptie- polymerasekettingreactie (RT-PCR) van een humane leukotrieenrecep- tor CYSLTRI-mRNA, omvattende een primer CysLTR1-F, een primer Cys- LTR1-R en een probe Cl-Probe, waarbij de primer CysLTR1-F een nu- cleotidesequentie heeft die wordt weergegeven in SEQ ID NO. 1, de primer CysLTRI-R een nuclecotidesequentie heeft die wordt weergege- ven in SEQ ID NO.2 en de probe Cl-Probe een nucleotidesequentie heeft die wordt weergegeven in SEQ ID NO.3.
2. Primer probe set volgens conclusie 1, waarbij een 5'-uiteinde van de probe Cl-probe is gelabeld met een fluorescerende reporter- groep, en een 3'-uiteinde van de probe Cl-probe is gelabeld met een quenchgroep.
3. Primer probe set volgens conclusie 1, verder omvattende een primer GAPDH-F, een primer GAPDH-R en een probe G-Probe van een referentiegen, waarbij de primer GAPDH-F een nucleotideseguentie heeft die wordt weergegeven in SEQ ID NO. 4, de primer GAPDH-R een nucleotidesequentie heeft die wordt weergegeven in SEQ ID NR. 5 en de probe G-Probe een nucleotidesequentie heeft die wordt weergege- ven in SEQ ID NR. 6.
4. Primer probe set volgens conclusie 3, waarbij een 5'-uiteinde van de probe G-Probe is gelabeld met een fluorescerende reporter- groep, en een 3'-uiteinde van de probe G-Probe is gelabeld met een quenchgroep; en de fluorescerende reportergroep die op de probe G- Probe is gelabeld, verschilt van de fluorescerende reportergroep die op de probe Cl-Probe is gelabeld.
5. Primer probe set volgens conclusie 2 of 4, waarbij de fluores- cerende reportergroep een 6-carboxyfluoresceïne (FAM) of een 2,7- dimethy1-4,5-dichloor-6-carboxyfluoresceïne (JOE) omvat, en de guenchgroep beseen Black Hole Quencher-1 (BHQ1) omvat.
6. Kit voor RT-PCR-detectie van een humane leukotrieenreceptor CYSLTRI-mRNA, omvattende de primer probe set volgens een van de conclusies 1 - 5, een PCR-reactieoplossing, een gemengde enzymop- lossing, een CysLTRl-standaard, een carboxy-X -rhodamine (ROX) re- ferentiekleurstof en nucleasevrij water.
7. Kit volgens conclusie 6, waarbij de PCR-reactieoplossing een mengsel van desoxy-ribonucleosidetrifosfaat (dNTP), MgCl; en een buffer omvat.
8. Kit volgens conclusie 6, waarbij de gemengde enzymoplossing een thermus aquaticus (Taq) enzym, een reverse transcriptase, een ri- bonuclease (RNase) remmer en een Tag-enzym antilichaam omvat.
9. Werkwijze voor het gebruik van de kit volgens conclusie 6, 7 of 8, omvattende de volgende stappen: mengen van de primer probe set, de PCR-reactieoplossing, de gemengde enzymoplossing, de CysLTR1- standaard of een te testen CysLTRl-monster, de ROX- referentiekleurstof en het nucleasevrije water, en het uitvoeren van kwantitatieve fluorescentieversterking.
10. Werkwijze volgens conclusie 9, waarbij op basis van 20 pL een reactiesysteem van de kit omvat: 2 pL van de primersondeset, 10 pL van de PCR-reactieoplossing, 0,5 uL van de gemengde enzymoplos- sing, 0,1 pL van de ROX-referentiekleurstof, 5 pL van de CysLTR1- standaard of het te testen CysLTRl-monster, en 2,4 HL van het nu- cleasevrije water; en waarbij de kwantitatieve fluorescentieamplificatie wordt uitge- voerd bij: 42 °C gedurende 30 minuten; 95 °C gedurende 1 minuut; 95 °C gedurende 5 s en 60 °C gedurende 31 s, gedurende 40 cycli.
NL2030976A 2021-08-04 2022-02-16 Primer probe set and kit for rt-pcr detection of human leukotriene receptor cysltr1 mrna NL2030976B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110890788.8A CN113564245A (zh) 2021-08-04 2021-08-04 一种人白三烯受体CysLTR1 mRNA RT-PCR检测用引物探针组和试剂盒

Publications (2)

Publication Number Publication Date
NL2030976A true NL2030976A (en) 2023-02-15
NL2030976B1 NL2030976B1 (en) 2023-03-14

Family

ID=78170341

Family Applications (1)

Application Number Title Priority Date Filing Date
NL2030976A NL2030976B1 (en) 2021-08-04 2022-02-16 Primer probe set and kit for rt-pcr detection of human leukotriene receptor cysltr1 mrna

Country Status (4)

Country Link
CN (1) CN113564245A (nl)
AU (1) AU2021106581A4 (nl)
NL (1) NL2030976B1 (nl)
ZA (1) ZA202200487B (nl)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2272977A1 (en) * 2009-07-08 2011-01-12 Universite Libre De Bruxelles Diagnostic method and kit for the detection of a lymphocytic variant of hypereosinophilic syndrome

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465212B1 (en) * 1999-09-20 2002-10-15 Schering Corporation Leukotriene receptor
US7666596B2 (en) * 2005-05-23 2010-02-23 University Of Alberta Tissue rejection
CN102121048A (zh) * 2010-01-11 2011-07-13 中山大学附属肿瘤医院 一种pool fq-pcr联合检测肿瘤耐药基因的方法及其试剂盒
CN110592290B (zh) * 2019-10-31 2022-10-04 上海市动物疫病预防控制中心 一种用于检测猫杯状病毒的试剂盒及检测方法
EP4289968A4 (en) * 2021-08-04 2024-06-26 Hangzhou Zheda Dixun Biological Gene Engineering Co., Ltd PRIMER-PROBE ASSEMBLY AND KIT FOR RT-PCR DETECTION OF HUMAN LEUKOTRIENE RECEPTOR CYSLTR1 MRNA
CN113388679A (zh) * 2021-08-04 2021-09-14 杭州浙大迪迅生物基因工程有限公司 一种人白三烯受体CysLTR2 mRNA RT-PCR检测用引物探针组和试剂盒

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2272977A1 (en) * 2009-07-08 2011-01-12 Universite Libre De Bruxelles Diagnostic method and kit for the detection of a lymphocytic variant of hypereosinophilic syndrome

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SHIRASAKI H ET AL: "Agonist- and T"H2 cytokine-induced up-regulation of cysteinyl leukotriene receptor messenger RNA in human monocytes", ANNALS OF ALLERGY, ASTHMA, ELSEVIER, AMSTERDAM, NL, vol. 99, no. 4, 1 October 2007 (2007-10-01), pages 340 - 347, XP026959892, ISSN: 1081-1206, [retrieved on 20071001] *
WEI SONG ET AL: "Antagonism of cysteinyl leukotriene receptor 1 (cysLTR1) by montelukast suppresses cell senescence of chondrocytes", CYTOKINE, vol. 103, 1 March 2018 (2018-03-01), US, pages 83 - 89, XP055584474, ISSN: 1043-4666, DOI: 10.1016/j.cyto.2017.12.021 *

Also Published As

Publication number Publication date
CN113564245A (zh) 2021-10-29
AU2021106581A4 (en) 2021-11-11
NL2030976B1 (en) 2023-03-14
ZA202200487B (en) 2022-03-30

Similar Documents

Publication Publication Date Title
CN103725798B (zh) 用于以rt-lamp法检测肾综合征出血热病毒的引物、试剂盒、检测方法
CN112538550B (zh) 基于RT-RPA和CRISPR/Cas的DHAV-1和DHAV-3检测体系及应用
WO2023010326A1 (zh) 一种人β-类胰蛋白酶mRNA RT-PCR检测用引物探针组和试剂盒
WO2023025259A1 (zh) 检测微小rna的方法和试剂盒
CN110724764A (zh) 人冠状病毒和呼吸道合胞病毒荧光定量pcr检测方法及其应用
NL2030975B1 (en) Primer probe set and kit for rt-pcr detection of human leukotriene receptor cysltr2 mrna
EP4289968A1 (en) Primer-probe set and kit for human leukotriene receptor cysltr1 mrna rt-pcr detection
NL2030976B1 (en) Primer probe set and kit for rt-pcr detection of human leukotriene receptor cysltr1 mrna
EP4306655A1 (en) Primer-probe set, kit and detection method for detecting human histamine receptor hrh1 mrna
AU2021106592A4 (en) Primer set for detection of expression of human eosinophil cationic protein (ecp) mrna and kit
NL2030977B1 (en) Reagent for detecting expression level of human histamine receptor hrh4 mrna, kit and use
US20240150832A1 (en) Reagent for detecting expression level of human histamine receptor hrh4 mrna, kit and detection method
AU2021106574A4 (en) Primer probe set for human histamine receptor hrh1 mrna detection, kit and use
NL2030974B1 (en) Primer probe set and kit for rt-pcr detection of human tryptase beta (tpsb) mrna
WO2023010327A1 (zh) 一种人白三烯受体CysLTR2 mRNA RT-PCR检测用引物探针组和试剂盒
CN111621557B (zh) 鸡尾酒式检测骨质疏松症易感基因的试剂盒及其使用方法
CN109609661B (zh) 一种肾阳虚外感小鼠模型肺组织qPCR内参基因组合及其筛选方法
WO2023010325A1 (zh) 一组检测人嗜酸性粒细胞阳离子蛋白mRNA表达的引物组、试剂盒和检测方法
CN115725702A (zh) 一种人内源性rna内标的荧光rt-pcr引物和探针、试剂盒及使用方法
CN115478116A (zh) 用于新型冠状病毒中重型、危重型病例早期预警的试剂盒
CN106554991A (zh) 可用于冠状动脉血管钙化诊断的血液miR-134检测试剂盒
CN115478117A (zh) 一种用于早期预警新型冠状病毒中重型、危重型病例的试剂盒
CN112899395A (zh) 一种新型冠状病毒核酸免提取的实时检测方法及其试剂盒
CN116640884A (zh) 用于gⅱ.6型诺如病毒检测的试剂组合物、试剂盒及方法
CN113122622A (zh) 一种通过piR-31925对弱精症诊断试剂盒及应用