MX386318B - Métodos y composiciones para caracterizar bacterias resistentes a fármacos a partir de muestras biológicas fijadas con formalina e incluidas en parafina. - Google Patents

Métodos y composiciones para caracterizar bacterias resistentes a fármacos a partir de muestras biológicas fijadas con formalina e incluidas en parafina.

Info

Publication number
MX386318B
MX386318B MX2018013791A MX2018013791A MX386318B MX 386318 B MX386318 B MX 386318B MX 2018013791 A MX2018013791 A MX 2018013791A MX 2018013791 A MX2018013791 A MX 2018013791A MX 386318 B MX386318 B MX 386318B
Authority
MX
Mexico
Prior art keywords
formalin
paraffin
compositions
methods
fixed
Prior art date
Application number
MX2018013791A
Other languages
English (en)
Other versions
MX2018013791A (es
Inventor
Daniel Jung
Kakuturu Rao
Yi Zhou
Original Assignee
American Molecular Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Molecular Laboratories Inc filed Critical American Molecular Laboratories Inc
Publication of MX2018013791A publication Critical patent/MX2018013791A/es
Publication of MX386318B publication Critical patent/MX386318B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La presente invención se refiere a métodos y composiciones generalmente útiles para el uso de la amplificación por reacción en cadena de la polimerasa (PCR) de secuencias de ADN trazas a partir de muestras de biopsia fijadas con formalina e incluidas en parafina (FFPE) y específicamente relevantes para la identificación de H. pylori resistente a múltiples fármacos en tales muestras de biopsia.
MX2018013791A 2016-05-10 2017-05-10 Métodos y composiciones para caracterizar bacterias resistentes a fármacos a partir de muestras biológicas fijadas con formalina e incluidas en parafina. MX386318B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662334185P 2016-05-10 2016-05-10
PCT/US2017/031901 WO2017196941A1 (en) 2016-05-10 2017-05-10 Methods and compositions for characterizing drug resistant bacteria from formalin-fixed paraffin-embedded biological samples

Publications (2)

Publication Number Publication Date
MX2018013791A MX2018013791A (es) 2019-08-05
MX386318B true MX386318B (es) 2025-03-18

Family

ID=60266713

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018013791A MX386318B (es) 2016-05-10 2017-05-10 Métodos y composiciones para caracterizar bacterias resistentes a fármacos a partir de muestras biológicas fijadas con formalina e incluidas en parafina.

Country Status (10)

Country Link
US (1) US10968489B2 (es)
EP (1) EP3455380B1 (es)
JP (1) JP7143284B2 (es)
KR (1) KR102326351B1 (es)
CN (1) CN109312410B (es)
AU (1) AU2017262776B2 (es)
CA (1) CA3023685A1 (es)
ES (1) ES2886270T3 (es)
MX (1) MX386318B (es)
WO (1) WO2017196941A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11708616B2 (en) * 2017-11-16 2023-07-25 American Molecular Laboratories Inc. Methods for detection of antibiotic resistant H. pylori
WO2020031190A1 (en) * 2018-08-08 2020-02-13 Tel Hashomer Medical Research, Infrastructure And Services Ltd. Molecular typing of microbes
CN109141963B (zh) * 2018-09-27 2023-12-19 上海海洋大学 一种同位素测定样品痕量取样及包埋器具
WO2020237237A1 (en) * 2019-05-23 2020-11-26 American Molecular Laboratories, Inc. Methods for detecting a level of h. pylori in a fecal sample
CN110184331A (zh) * 2019-05-27 2019-08-30 深圳前海大井医疗股份有限公司 幽门螺杆菌阴阳性与克拉霉素耐药性同步检测法
CN110423799A (zh) * 2019-08-12 2019-11-08 复旦大学 一种幽门螺杆菌左氧氟沙星耐药检测方法
CN115786551A (zh) * 2022-11-23 2023-03-14 济南艾迪康医学检验中心有限公司 检测四环素治疗幽门螺杆菌感染的耐药基因16s rna突变情况的引物、试剂盒和方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040052799A1 (en) 1996-11-15 2004-03-18 Astra Aktiebolag Nucleic acid and amino acid sequences relating to Helicobacter pylori for diagnostics and therapeutics
JP2003516151A (ja) * 1999-11-29 2003-05-13 エイブイアイ バイオファーマ， インコーポレイテッド 細菌１６Ｓおよび２３ＳｒＲＮＡに標的化された、荷電していないアンチセンスオリゴヌクレオチド、ならびにその使用
EP2633311A4 (en) * 2010-10-26 2014-05-07 Univ Stanford NONINVASIVE FETAL GENETIC SCREENING THROUGH SEQUENCING ANALYSIS
US20130059762A1 (en) * 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
US9957558B2 (en) * 2011-04-28 2018-05-01 Life Technologies Corporation Methods and compositions for multiplex PCR
WO2015095225A1 (en) * 2013-12-19 2015-06-25 The Board Of Trustees Of The Leland Stanford Junior University Quantification of mutant alleles and copy number variation using digital pcr with nonspecific dna-binding dyes
WO2016053883A1 (en) * 2014-10-03 2016-04-07 Life Technologies Corporation Detection, identification, validation and enrichment of target nucleic acids
CN104846097B (zh) * 2015-05-21 2017-12-22 杭州千基生物科技有限公司 幽门螺旋杆菌分型与耐药突变基因检测试剂盒
CN105368825B (zh) * 2015-10-14 2019-09-10 华东医院 幽门螺杆菌抗生素耐药性分析试剂盒及耐药性检测方法

Also Published As

Publication number Publication date
US10968489B2 (en) 2021-04-06
KR102326351B1 (ko) 2021-11-12
WO2017196941A1 (en) 2017-11-16
KR20190003739A (ko) 2019-01-09
JP7143284B2 (ja) 2022-09-28
AU2017262776A1 (en) 2018-12-06
CN109312410B (zh) 2022-10-28
EP3455380A1 (en) 2019-03-20
US20170327873A1 (en) 2017-11-16
EP3455380A4 (en) 2020-03-11
CA3023685A1 (en) 2017-11-16
ES2886270T3 (es) 2021-12-16
MX2018013791A (es) 2019-08-05
JP2019514438A (ja) 2019-06-06
CN109312410A (zh) 2019-02-05
EP3455380B1 (en) 2021-07-07
AU2017262776B2 (en) 2023-07-06

Similar Documents

Publication Publication Date Title
MX386318B (es) Métodos y composiciones para caracterizar bacterias resistentes a fármacos a partir de muestras biológicas fijadas con formalina e incluidas en parafina.
MY205579A (en) Nuclease-based rna depletion
WO2016064894A3 (en) Compositions and methods for detecting an rna virus
HK1256342A1 (zh) 环境或生物样本中的核酸纯化
EP3845640A3 (en) Modified tranposases for improved insertion sequence bias and increased dna input tolerence
EP4624589A3 (en) Methods and systems for processing polynucleotides
EA201692157A1 (ru) Синтез двухцепочечных нуклеиновых кислот
WO2015081229A3 (en) Selective amplification of nucleic acid sequences
MX2017013878A (es) Amplificación de discordancias optimizadas multiplicadas (moma) - reacción en cadena de polimerasa (pcr) en tiempo real, para evaluar el ácido desoxirribonucleico (adn) libre de celula.
WO2015195949A3 (en) Methods, compositions, and devices for rapid analysis of biological markers
SG11201809961TA (en) Variants of a dna polymerase of the polx family
MX376755B (es) Detección de ácidos nucleicos diana mediante el uso de hibridación.
EA201170933A1 (ru) Анализ нуклеиновых кислот, полученных из единичных клеток
MX375482B (es) Composición y método para estabilizar ácidos nucleicos en muestras biológicas.
NZ723570A (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
AR067135A1 (es) Metodos para aislar arn de fragmento largo de muestras fijas
EP4491740A3 (en) Analysis of chromatin using a nicking enzyme
WO2016109799A3 (en) Method of rna isolation from clinical samples
MX2016014292A (es) Composiciones y metodos para detectar la enfermedad del dragon amarillo (huanglongbing).
EP3795681A3 (en) Polymerase compositions and methods of making and using same
WO2014143714A3 (en) Methods for one step nucleic acid amplification of non-eluted samples
NZ732886A (en) Amplification and detection of nucleic acids in a biological sample
EP4421184A3 (en) Methods and reagents for molecular barcoding
MX394156B (es) Molecula de sustrato.
MX366113B (es) Metodos de diganostico y composiciones.