MX380248B - Partículas de transducción no replicativas y sistemas indicadores a base de partículas de transducción. - Google Patents
Partículas de transducción no replicativas y sistemas indicadores a base de partículas de transducción.Info
- Publication number
- MX380248B MX380248B MX2018013417A MX2018013417A MX380248B MX 380248 B MX380248 B MX 380248B MX 2018013417 A MX2018013417 A MX 2018013417A MX 2018013417 A MX2018013417 A MX 2018013417A MX 380248 B MX380248 B MX 380248B
- Authority
- MX
- Mexico
- Prior art keywords
- reporter
- transduction
- replicative
- particles
- systems
- Prior art date
Links
- 230000026683 transduction Effects 0.000 title abstract 6
- 238000010361 transduction Methods 0.000 title abstract 6
- 239000002245 particle Substances 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 230000003362 replicative effect Effects 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 2
- 108700008625 Reporter Genes Proteins 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 238000004806 packaging method and process Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/14—Streptococcus; Staphylococcus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10111—Myoviridae
- C12N2795/10141—Use of virus, viral particle or viral elements as a vector
- C12N2795/10143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10111—Myoviridae
- C12N2795/10151—Methods of production or purification of viral material
- C12N2795/10152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10311—Siphoviridae
- C12N2795/10341—Use of virus, viral particle or viral elements as a vector
- C12N2795/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10311—Siphoviridae
- C12N2795/10351—Methods of production or purification of viral material
- C12N2795/10352—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
- G01N2333/31—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361779177P | 2013-03-13 | 2013-03-13 | |
| US201361897040P | 2013-10-29 | 2013-10-29 | |
| US201461939126P | 2014-02-12 | 2014-02-12 | |
| PCT/US2014/026536 WO2014160418A2 (en) | 2013-03-13 | 2014-03-13 | Non-replicative transduction particles and transduction particle-based reporter systems |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX380248B true MX380248B (es) | 2025-03-12 |
Family
ID=51625644
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015011446A MX360560B (es) | 2013-03-13 | 2014-03-13 | Partículas de transducción no replicativas y sistemas indicadores a base de partículas de transducción. |
| MX2018013417A MX380248B (es) | 2013-03-13 | 2014-03-13 | Partículas de transducción no replicativas y sistemas indicadores a base de partículas de transducción. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015011446A MX360560B (es) | 2013-03-13 | 2014-03-13 | Partículas de transducción no replicativas y sistemas indicadores a base de partículas de transducción. |
Country Status (14)
| Country | Link |
|---|---|
| US (5) | US9388453B2 (enExample) |
| EP (2) | EP3699592A1 (enExample) |
| JP (2) | JP6431035B2 (enExample) |
| KR (1) | KR102127358B1 (enExample) |
| CN (2) | CN105378060B (enExample) |
| AU (1) | AU2014243796B2 (enExample) |
| BR (1) | BR112015023108A8 (enExample) |
| CA (1) | CA2905341C (enExample) |
| DK (1) | DK2968424T3 (enExample) |
| ES (1) | ES2780827T3 (enExample) |
| MX (2) | MX360560B (enExample) |
| RU (1) | RU2661101C2 (enExample) |
| SG (1) | SG11201507463WA (enExample) |
| WO (1) | WO2014160418A2 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3699592A1 (en) | 2013-03-13 | 2020-08-26 | Geneweave Biosciences Inc. | Non-replicative transduction particles and transduction particle-based reporter systems |
| US9481903B2 (en) | 2013-03-13 | 2016-11-01 | Roche Molecular Systems, Inc. | Systems and methods for detection of cells using engineered transduction particles |
| US9540675B2 (en) | 2013-10-29 | 2017-01-10 | GeneWeave Biosciences, Inc. | Reagent cartridge and methods for detection of cells |
| EP3099173A4 (en) | 2014-01-29 | 2017-09-20 | Synphagen LLC. | Therapeutic phages and methods for delivery of nucleic acids for therapeutic uses |
| SG11201610430QA (en) * | 2014-06-13 | 2017-01-27 | Geneweave Biosciences Inc | Growth-independent detection of cells |
| KR101946180B1 (ko) * | 2014-08-25 | 2019-02-08 | 지니위브 바이오사이언시스, 인코포레이티드 | 비-복제 형질도입 입자 및 형질도입 입자-기반 리포터 시스템 |
| US9879328B2 (en) | 2014-11-21 | 2018-01-30 | GeneWeave Biosciences, Inc. | Mechanisms of antimicrobial susceptibility |
| US10227661B2 (en) | 2014-11-21 | 2019-03-12 | GeneWeave Biosciences, Inc. | Sequence-specific detection and phenotype determination |
| US10351893B2 (en) | 2015-10-05 | 2019-07-16 | GeneWeave Biosciences, Inc. | Reagent cartridge for detection of cells |
| US10718003B2 (en) * | 2015-12-31 | 2020-07-21 | Roche Molecular Systems, Inc. | Detecting an analyte in a flash and glow reaction |
| WO2017172644A2 (en) | 2016-03-28 | 2017-10-05 | The Charles Stark Draper Laboratory, Inc. | Bacteria identification and antibiotic susceptibility profiling device |
| US11149319B2 (en) | 2016-12-20 | 2021-10-19 | Roche Molecular Systems, Inc. | Systems and methods for detecting cells using engineered transduction particles |
| CN111601897A (zh) | 2016-12-30 | 2020-08-28 | 奎多公司 | 用于确定细菌对抗生素或益菌剂的易感性的噬菌体介导的免疫分析和方法 |
| US11149269B2 (en) | 2017-05-15 | 2021-10-19 | New York University | Compositions and methods for non-antibiotic treating of bacterial infections by blocking or disrupting bacterial genes involved in virulence or viability |
| US11077444B2 (en) | 2017-05-23 | 2021-08-03 | Roche Molecular Systems, Inc. | Packaging for a molecular diagnostic cartridge |
| US11008602B2 (en) * | 2017-12-20 | 2021-05-18 | Roche Molecular Systems, Inc. | Non-replicative transduction particles and transduction particle-based reporter systems |
| WO2019209982A1 (en) * | 2018-04-24 | 2019-10-31 | Laboratory Corporation Of America Holdings | Indicator bacteriophage for selecting and monitoring for efficacy of therapeutics and methods for using same |
| US11541105B2 (en) | 2018-06-01 | 2023-01-03 | The Research Foundation For The State University Of New York | Compositions and methods for disrupting biofilm formation and maintenance |
| US10968491B2 (en) | 2018-12-05 | 2021-04-06 | GeneWeave Biosciences, Inc. | Growth-independent detection of cells |
| JP2022515451A (ja) | 2018-12-27 | 2022-02-18 | エフ.ホフマン-ラ ロシュ アーゲー | アシネトバクター・バウマンニの検出のための、非複製的形質導入粒子及び形質導入粒子に基づくレポーター系 |
| CN113227403A (zh) * | 2018-12-29 | 2021-08-06 | 豪夫迈·罗氏有限公司 | 含铁抗菌素类在抗生素敏感性测定中用于限制交叉反应性以及改善细菌鉴定之用途 |
| US11572595B2 (en) | 2018-12-31 | 2023-02-07 | Roche Molecular Systems, Inc. | Non-replicative transduction particles with one or more non-native tail fibers and transduction particle-based reporter systems |
| WO2020245123A1 (en) | 2019-06-06 | 2020-12-10 | F. Hoffmann-La Roche Ag | Use of peptidomimetic antimicrobial peptides to limit cross-reactivity and improve bacterial identification in antibiotic susceptibility assays |
| JP7781741B2 (ja) | 2019-08-26 | 2025-12-08 | ラボラトリー コーポレイション オブ アメリカ ホールディングス | 組換え繁殖欠損指標バクテリオファージを使用して微生物を検出するためのデバイスおよび方法 |
| US20220356537A1 (en) * | 2019-12-31 | 2022-11-10 | Roche Molecular Systems, Inc. | Quantitative pcr screening of inducible prophage from bacterial isolates |
| JP2024532062A (ja) * | 2021-08-20 | 2024-09-05 | ユニバーシティ オブ ピッツバーグ-オブ ザ コモンウェルス システム オブ ハイヤー エデュケーション | 抗生物質耐性サンプルと抗生物質感受性サンプルとの識別 |
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| BE628679A (enExample) | 1962-02-28 | |||
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| CA1277931C (en) | 1984-06-05 | 1990-12-18 | Shimon Ulitzur | Detection and/or identification of microorganisms in a test sample usingbioluminescence or other exogenous genetically-introduced marker |
| US4861709A (en) | 1985-05-31 | 1989-08-29 | Technicon Research A.G. | Detection and/or identification of microorganisms in a test sample using bioluminescence or other exogenous genetically-introduced marker |
| WO1987000201A1 (en) | 1985-07-05 | 1987-01-15 | Whitehead Institute For Biomedical Research | Epithelial cells expressing foreign genetic material |
| US4980286A (en) | 1985-07-05 | 1990-12-25 | Whitehead Institute For Biomedical Research | In vivo introduction and expression of foreign genetic material in epithelial cells |
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| EP0633318A1 (en) | 1987-09-11 | 1995-01-11 | Whitehead Institute For Biomedical Research | Transduced fibroblasts and uses therefor |
| DE3851153T2 (de) | 1987-12-11 | 1995-01-05 | Hughes Howard Med Inst | Genetische modifizierung von endothelialen zellen. |
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| FR2641793B1 (fr) | 1988-12-26 | 1993-10-01 | Setratech | Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases |
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| JPH0634546A (ja) | 1992-07-17 | 1994-02-08 | Tosoh Corp | 蛍光検出器 |
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| US5494646A (en) | 1993-04-14 | 1996-02-27 | Seymour; Eugene H. | Sampling device and sample adequacy system |
| WO1994025572A1 (en) * | 1993-04-29 | 1994-11-10 | Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University | Mycobacterial species-specific reporter mycobacteriophages |
| US5645801A (en) | 1993-10-21 | 1997-07-08 | Abbott Laboratories | Device and method for amplifying and detecting target nucleic acids |
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