MX352068B - Promotores de regulacion inducibles. - Google Patents

Promotores de regulacion inducibles.

Info

Publication number
MX352068B
MX352068B MX2013001037A MX2013001037A MX352068B MX 352068 B MX352068 B MX 352068B MX 2013001037 A MX2013001037 A MX 2013001037A MX 2013001037 A MX2013001037 A MX 2013001037A MX 352068 B MX352068 B MX 352068B
Authority
MX
Mexico
Prior art keywords
regulation
inducible promoters
host cell
bacterial host
inable
Prior art date
Application number
MX2013001037A
Other languages
English (en)
Other versions
MX2013001037A (es
Inventor
Altenbuchner Josef
Kiziak Christoph
Wenzel Marian
Original Assignee
Lonza Ag Star
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP10171252A external-priority patent/EP2412813A1/en
Application filed by Lonza Ag Star filed Critical Lonza Ag Star
Publication of MX2013001037A publication Critical patent/MX2013001037A/es
Publication of MX352068B publication Critical patent/MX352068B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere a la producción de polipéptidos heterólogos en una célula huésped bacteriana recombinante, donde la célula huésped bacteriano se vuelve incapaz de desactivar el promotor que controla la expresión del polipéptido heterólogo en ausencia de un inductor.
MX2013001037A 2010-07-29 2011-07-27 Promotores de regulacion inducibles. MX352068B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10171252A EP2412813A1 (en) 2010-07-29 2010-07-29 Regulation of inducible promoters
EP11162420 2011-04-14
PCT/EP2011/062921 WO2012013710A2 (en) 2010-07-29 2011-07-27 Regulation of inducible promoters

Publications (2)

Publication Number Publication Date
MX2013001037A MX2013001037A (es) 2013-04-03
MX352068B true MX352068B (es) 2017-11-08

Family

ID=44533478

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013001037A MX352068B (es) 2010-07-29 2011-07-27 Promotores de regulacion inducibles.

Country Status (19)

Country Link
US (1) US9464294B2 (es)
EP (2) EP2722390B1 (es)
JP (1) JP6071878B2 (es)
KR (2) KR102022884B1 (es)
CN (2) CN103108954B (es)
AU (1) AU2011284844B9 (es)
BR (1) BR112013003657A2 (es)
CA (1) CA2803654C (es)
DK (1) DK2722390T3 (es)
EA (2) EA034074B1 (es)
ES (1) ES2636910T3 (es)
HK (1) HK1184496A1 (es)
IL (1) IL223864B (es)
MX (1) MX352068B (es)
PL (1) PL2722390T3 (es)
SG (1) SG187024A1 (es)
TW (2) TWI515295B (es)
WO (1) WO2012013710A2 (es)
ZA (1) ZA201209268B (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9970017B2 (en) * 2013-03-27 2018-05-15 Lonza Ag Regulation of inducible promoters
CN104404135B (zh) * 2014-10-27 2017-12-29 首都师范大学 实时荧光定量PCR定位基因转录起始位点和PolyA位点的方法
KR102239307B1 (ko) * 2020-06-29 2021-04-12 한국생명공학연구원 포도당에 의해 조절되는 프로모터 변이체 및 이를 이용한 단백질 생산 방법
CN112062822B (zh) * 2020-09-24 2022-02-01 江南大学 一种碳分解代谢调控蛋白CcpA突变体I42A

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006255060B2 (en) 2005-06-06 2012-08-30 Pelican Technology Holdings, Inc. Mannitol induced promoter systems in bacterial host cells
DK2284273T3 (da) * 2009-08-10 2013-01-21 Lonza Ag Vektor omfattende en mannosepromotor samt mannosepromotor
EP2292769A1 (en) * 2009-08-10 2011-03-09 Lonza AG Fermentation process

Also Published As

Publication number Publication date
EP2598640B1 (en) 2015-04-01
CN103108954B (zh) 2015-05-13
ZA201209268B (en) 2013-07-31
IL223864B (en) 2018-05-31
HK1184496A1 (en) 2014-01-24
CA2803654C (en) 2021-02-16
CN103108954A (zh) 2013-05-15
TWI510623B (zh) 2015-12-01
EA201600029A1 (ru) 2016-05-31
EP2598640A2 (en) 2013-06-05
EP2722390B1 (en) 2017-05-10
BR112013003657A2 (pt) 2017-02-21
TW201213544A (en) 2012-04-01
DK2722390T3 (en) 2017-08-28
ES2636910T3 (es) 2017-10-10
EP2722390A1 (en) 2014-04-23
EA034074B1 (ru) 2019-12-25
MX2013001037A (es) 2013-04-03
KR20180086261A (ko) 2018-07-30
US9464294B2 (en) 2016-10-11
AU2011284844B9 (en) 2015-10-29
TWI515295B (zh) 2016-01-01
KR101904140B1 (ko) 2018-10-04
KR102022884B1 (ko) 2019-09-20
US20130203114A1 (en) 2013-08-08
JP6071878B2 (ja) 2017-02-01
JP2013535197A (ja) 2013-09-12
PL2722390T3 (pl) 2017-12-29
AU2011284844B2 (en) 2015-10-15
EA024834B1 (ru) 2016-10-31
EA201300189A1 (ru) 2013-05-30
WO2012013710A3 (en) 2012-04-26
TW201428102A (zh) 2014-07-16
AU2011284844A1 (en) 2013-01-10
WO2012013710A2 (en) 2012-02-02
CN105200100A (zh) 2015-12-30
SG187024A1 (en) 2013-02-28
CA2803654A1 (en) 2012-02-02
KR20130054328A (ko) 2013-05-24

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