MX346117B - Metodo para produccion mejorada de proteinas en hongo filamentoso. - Google Patents

Metodo para produccion mejorada de proteinas en hongo filamentoso.

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Publication number
MX346117B
MX346117B MX2012014138A MX2012014138A MX346117B MX 346117 B MX346117 B MX 346117B MX 2012014138 A MX2012014138 A MX 2012014138A MX 2012014138 A MX2012014138 A MX 2012014138A MX 346117 B MX346117 B MX 346117B
Authority
MX
Mexico
Prior art keywords
protein production
improved protein
filamentous fungi
relates
filamentous fungus
Prior art date
Application number
MX2012014138A
Other languages
English (en)
Other versions
MX2012014138A (es
Inventor
Pakula Tiina
Saloheimo Markku
Häkkinen Mari
Westerholm-Parvinen Ann
Penttilä Merja
Vitikainen Marika
Original Assignee
Roal Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roal Oy filed Critical Roal Oy
Publication of MX2012014138A publication Critical patent/MX2012014138A/es
Publication of MX346117B publication Critical patent/MX346117B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/244—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Abstract

La presente invención se relaciona a un método para modificar genéticamente un huésped de hongo filamentoso para producción mejorada de proteína. El método comprende un huésped de hongo filamentoso que es modificado genéticamente para sobreexpresar o para ser deficiente de genes específicos. La invención se relaciona los huéspedes modificados. Adicionalmente, la invención se relaciona a un método para producción mejorada o para producir una composición mejorada de proteínas, como las celulasas, hemicelulasas, otras proteínas implicadas en la degradación de material lignocelulósico u otras proteínas, en un huésped de hongo filamentoso.
MX2012014138A 2010-06-04 2011-05-30 Metodo para produccion mejorada de proteinas en hongo filamentoso. MX346117B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20105633A FI20105633A0 (fi) 2010-06-04 2010-06-04 Parannettu menetelmä proteiinien tuottamiseksi
PCT/FI2011/050495 WO2011151513A1 (en) 2010-06-04 2011-05-30 Method for improved protein production in filamentous fungi

Publications (2)

Publication Number Publication Date
MX2012014138A MX2012014138A (es) 2013-05-20
MX346117B true MX346117B (es) 2017-03-08

Family

ID=42308090

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012014138A MX346117B (es) 2010-06-04 2011-05-30 Metodo para produccion mejorada de proteinas en hongo filamentoso.

Country Status (10)

Country Link
US (3) US9487791B2 (es)
EP (2) EP2576793B1 (es)
CN (1) CN103154249B (es)
BR (1) BR112012030919A2 (es)
CA (1) CA2801599C (es)
DK (2) DK2733210T3 (es)
ES (1) ES2659946T3 (es)
FI (1) FI20105633A0 (es)
MX (1) MX346117B (es)
WO (1) WO2011151513A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104087568A (zh) * 2014-06-11 2014-10-08 华南理工大学 一种制浆造纸漂白预处理用半纤维素酶的制备和应用
CN106119137B (zh) * 2015-05-06 2020-06-26 中国科学院天津工业生物技术研究所 一种改善丝状真菌蛋白分泌能力的方法
CN106190874B (zh) * 2015-05-06 2020-06-26 中国科学院天津工业生物技术研究所 一种强化丝状真菌蛋白质生产的方法
WO2017177289A1 (en) 2016-04-15 2017-10-19 Vtt International Oy Process for conversion of biomass into fermentable sugars with integrated enzyme
WO2019165063A1 (en) * 2018-02-23 2019-08-29 Novozymes A/S Long non-coding rna-expression in fungal hosts
EP3981882A1 (en) * 2020-10-08 2022-04-13 Clariant Produkte (Deutschland) GmbH Process for the production of a filamentous fungus whole broth enzyme composition with low viscosity
CN117716039A (zh) 2021-07-19 2024-03-15 丹尼斯科美国公司 用于增强真菌细胞中的蛋白质产生的组合物和方法
WO2023102315A1 (en) 2021-12-01 2023-06-08 Danisco Us Inc. Compositions and methods for enhanced protein production in fungal cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60133884D1 (de) 2000-03-24 2008-06-19 Genencor Int Herstellung von sekretierten proteinen durch rekombinante eukaryotische zellen
EP2082047A2 (en) * 2006-08-29 2009-07-29 Danisco US, INC., Genencor Division Compositions and methods for improved protein production
US20110045526A1 (en) * 2007-06-15 2011-02-24 Bodie Elizabeth A Selection of useful fungal strains

Also Published As

Publication number Publication date
MX2012014138A (es) 2013-05-20
CN103154249B (zh) 2015-12-02
ES2659946T3 (es) 2018-03-20
DK2733210T3 (en) 2018-03-12
DK2576793T3 (en) 2017-07-10
CN103154249A (zh) 2013-06-12
EP2733210A1 (en) 2014-05-21
US20130084604A1 (en) 2013-04-04
US20150307862A1 (en) 2015-10-29
CA2801599C (en) 2019-01-15
US9695430B2 (en) 2017-07-04
WO2011151513A1 (en) 2011-12-08
BR112012030919A2 (pt) 2018-08-28
EP2733210B1 (en) 2017-12-06
EP2576793A1 (en) 2013-04-10
FI20105633A0 (fi) 2010-06-04
CA2801599A1 (en) 2011-12-08
US9487791B2 (en) 2016-11-08
US20160108409A1 (en) 2016-04-21
EP2576793B1 (en) 2017-03-15

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