MX2012014138A - Metodo para produccion mejorada de proteinas en hongo filamentoso. - Google Patents

Metodo para produccion mejorada de proteinas en hongo filamentoso.

Info

Publication number
MX2012014138A
MX2012014138A MX2012014138A MX2012014138A MX2012014138A MX 2012014138 A MX2012014138 A MX 2012014138A MX 2012014138 A MX2012014138 A MX 2012014138A MX 2012014138 A MX2012014138 A MX 2012014138A MX 2012014138 A MX2012014138 A MX 2012014138A
Authority
MX
Mexico
Prior art keywords
protein production
improved protein
filamentous fungi
relates
filamentous fungus
Prior art date
Application number
MX2012014138A
Other languages
English (en)
Other versions
MX346117B (es
Inventor
Tiina Pakula
Markku Saloheimo
Mari Haekkinen
Ann Westerholm-Parvinen
Merja Penttilae
Marika Vitikainen
Original Assignee
Teknologian Tutkimuskeskus Vtt Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Teknologian Tutkimuskeskus Vtt Oy filed Critical Teknologian Tutkimuskeskus Vtt Oy
Publication of MX2012014138A publication Critical patent/MX2012014138A/es
Publication of MX346117B publication Critical patent/MX346117B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/244—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se relaciona a un método para modificar genéticamente un huésped de hongo filamentoso para producción mejorada de proteína. El método comprende un huésped de hongo filamentoso que es modificado genéticamente para sobreexpresar o para ser deficiente de genes específicos. La invención se relaciona los huéspedes modificados. Adicionalmente, la invención se relaciona a un método para producción mejorada o para producir una composición mejorada de proteínas, como las celulasas, hemicelulasas, otras proteínas implicadas en la degradación de material lignocelulósico u otras proteínas, en un huésped de hongo filamentoso.
MX2012014138A 2010-06-04 2011-05-30 Metodo para produccion mejorada de proteinas en hongo filamentoso. MX346117B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20105633A FI20105633A0 (fi) 2010-06-04 2010-06-04 Parannettu menetelmä proteiinien tuottamiseksi
PCT/FI2011/050495 WO2011151513A1 (en) 2010-06-04 2011-05-30 Method for improved protein production in filamentous fungi

Publications (2)

Publication Number Publication Date
MX2012014138A true MX2012014138A (es) 2013-05-20
MX346117B MX346117B (es) 2017-03-08

Family

ID=42308090

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012014138A MX346117B (es) 2010-06-04 2011-05-30 Metodo para produccion mejorada de proteinas en hongo filamentoso.

Country Status (10)

Country Link
US (3) US9487791B2 (es)
EP (2) EP2576793B1 (es)
CN (1) CN103154249B (es)
BR (1) BR112012030919A2 (es)
CA (1) CA2801599C (es)
DK (2) DK2733210T3 (es)
ES (1) ES2659946T3 (es)
FI (1) FI20105633A0 (es)
MX (1) MX346117B (es)
WO (1) WO2011151513A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104087568A (zh) * 2014-06-11 2014-10-08 华南理工大学 一种制浆造纸漂白预处理用半纤维素酶的制备和应用
CN106190874B (zh) * 2015-05-06 2020-06-26 中国科学院天津工业生物技术研究所 一种强化丝状真菌蛋白质生产的方法
CN106119137B (zh) * 2015-05-06 2020-06-26 中国科学院天津工业生物技术研究所 一种改善丝状真菌蛋白分泌能力的方法
WO2017177289A1 (en) 2016-04-15 2017-10-19 Vtt International Oy Process for conversion of biomass into fermentable sugars with integrated enzyme
CN112105740A (zh) * 2018-02-23 2020-12-18 诺维信公司 真菌宿主中的长链非编码rna表达
EP3981882A1 (en) * 2020-10-08 2022-04-13 Clariant Produkte (Deutschland) GmbH Process for the production of a filamentous fungus whole broth enzyme composition with low viscosity
WO2023003683A2 (en) 2021-07-19 2023-01-26 Danisco Us Inc. Compositions and methods for enhanced protein production in fungal cells
WO2023102315A1 (en) 2021-12-01 2023-06-08 Danisco Us Inc. Compositions and methods for enhanced protein production in fungal cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1266018T3 (da) * 2000-03-24 2008-09-01 Genencor Int Fremstilling af udskilte proteiner af rekombinante, eukaryotiske celler
WO2008027472A2 (en) * 2006-08-29 2008-03-06 Danisco Us, Inc., Genencor Division Compositions and methods for improved protein production
WO2008156605A1 (en) * 2007-06-15 2008-12-24 Danisco Us, Inc., Genencor Division Selection of useful fungal strains

Also Published As

Publication number Publication date
DK2576793T3 (en) 2017-07-10
FI20105633A0 (fi) 2010-06-04
US20160108409A1 (en) 2016-04-21
EP2576793B1 (en) 2017-03-15
US9487791B2 (en) 2016-11-08
EP2576793A1 (en) 2013-04-10
US9695430B2 (en) 2017-07-04
US20150307862A1 (en) 2015-10-29
WO2011151513A1 (en) 2011-12-08
CN103154249A (zh) 2013-06-12
EP2733210B1 (en) 2017-12-06
BR112012030919A2 (pt) 2018-08-28
US20130084604A1 (en) 2013-04-04
CA2801599C (en) 2019-01-15
DK2733210T3 (en) 2018-03-12
MX346117B (es) 2017-03-08
CA2801599A1 (en) 2011-12-08
ES2659946T3 (es) 2018-03-20
CN103154249B (zh) 2015-12-02
EP2733210A1 (en) 2014-05-21

Similar Documents

Publication Publication Date Title
MX2012014138A (es) Metodo para produccion mejorada de proteinas en hongo filamentoso.
IN2014CN02100A (es)
WO2011151512A3 (en) Method for protein production in filamentous fungi
EA201201125A1 (ru) Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала
WO2013166469A8 (en) Methods for treating lignocellulosic materials
EA201300074A1 (ru) Способ получения представляющего интерес соединения
MX2014001383A (es) Colageno 7 y metodos relacionados.
MX367859B (es) Metodos y materiales para la biosintesis de compuestos mogrosidos.
MX2013007853A (es) Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos.
MX346700B (es) Metodo para mejorar el rendimiento de un polipeptido.
WO2013003744A3 (en) Bioconversion process for producing nylon-7, nylon-7,7 and polyesters
MX337919B (es) Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos.
MX2011013700A (es) Cepa talaromyces y composiciones de enzimas.
WO2013096898A3 (en) Bioconversion process for producing nylon-7, nylon-7,7 and polyesters
MX337913B (es) Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican los mismos.
WO2013013017A3 (en) Compositions and methods for modifying the glycosylation of lysosomal storage disorder therapeutics
MX2022002057A (es) Variantes de enzima con propiedades de ester sintasa mejoradas.
BR112014004193A2 (pt) célula hospedeira recombinante de trichoderma, métodos para produzir uma composição enzimática, composição enzimática, processos para degradar um material celulósico, para sintetizar um produto de fermentação e para fermentar um material celulósico
IN2015DN03206A (es)
MX2014001594A (es) Polipeptidos que tienen actividad de celulasa y polinucleotidos que los codifican.
WO2012136898A3 (en) Novel cutinases, their production and uses
WO2015051342A3 (en) Compositions and methods for modulating biomass productivity
WO2011151515A3 (en) Improved production of proteins in filamentous fungi
WO2013103246A3 (ko) 퀴놀린산을 생산하는 재조합 미생물 및 이를 이용한 퀴놀린산의 생산 방법
MX2013009403A (es) Agrupamiento genico para la biosintesis de cicloclavina.

Legal Events

Date Code Title Description
GB Transfer or assignment

Owner name: ROAL OY

FG Grant or registration