MX2023001192A - Mejoras en soluciones de lavado para cromatografia de intercambio anionico en un metodo de purificacion de proteinas rsv producidas de manera recombinante. - Google Patents
Mejoras en soluciones de lavado para cromatografia de intercambio anionico en un metodo de purificacion de proteinas rsv producidas de manera recombinante.Info
- Publication number
- MX2023001192A MX2023001192A MX2023001192A MX2023001192A MX2023001192A MX 2023001192 A MX2023001192 A MX 2023001192A MX 2023001192 A MX2023001192 A MX 2023001192A MX 2023001192 A MX2023001192 A MX 2023001192A MX 2023001192 A MX2023001192 A MX 2023001192A
- Authority
- MX
- Mexico
- Prior art keywords
- anion exchange
- exchange chromatography
- purification
- recombinantly
- rsv protein
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 7
- 108090000623 proteins and genes Proteins 0.000 title abstract 7
- 238000005571 anion exchange chromatography Methods 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 238000000746 purification Methods 0.000 title abstract 2
- 239000012501 chromatography medium Substances 0.000 abstract 4
- 239000000825 pharmaceutical preparation Substances 0.000 abstract 1
- 229940127557 pharmaceutical product Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18511—Pneumovirus, e.g. human respiratory syncytial virus
- C12N2760/18522—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18511—Pneumovirus, e.g. human respiratory syncytial virus
- C12N2760/18551—Methods of production or purification of viral material
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
La invención se refiere a un método de purificación de una proteína RSV, en donde una solución de carga que comprende la proteína RSV se pone en contacto con un medio de cromatografía de intercambio aniónico, mediante el cual la proteína RSV se une al medio de cromatografía de intercambio aniónico, el medio de cromatografía de intercambio aniónico es lavado con al menos al menos una solución de lavado y la proteína RSV es eluida del medio de cromatografía de intercambio aniónico. De acuerdo con la invención, la solución de lavado tiene un pH entre 3.0 y 6.5, por lo que se mejora la eliminación de las proteínas de célula huésped. La invención también está dirigida a un producto farmacéutico que incluye una proteína RSV purificada por dicho método.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063056949P | 2020-07-27 | 2020-07-27 | |
| PCT/IB2021/056629 WO2022023895A1 (en) | 2020-07-27 | 2021-07-22 | Improvements to wash solutions for anion exchange chromatography in a method of purification of recombinantly-produced rsv proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2023001192A true MX2023001192A (es) | 2023-02-22 |
Family
ID=77168315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2023001192A MX2023001192A (es) | 2020-07-27 | 2021-07-22 | Mejoras en soluciones de lavado para cromatografia de intercambio anionico en un metodo de purificacion de proteinas rsv producidas de manera recombinante. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20230287041A1 (es) |
| EP (1) | EP4188414A1 (es) |
| JP (1) | JP2022023813A (es) |
| CN (1) | CN116194464A (es) |
| AU (1) | AU2021315047A1 (es) |
| BR (1) | BR112023000638A2 (es) |
| CA (1) | CA3190141A1 (es) |
| IL (1) | IL299808A (es) |
| MX (1) | MX2023001192A (es) |
| WO (1) | WO2022023895A1 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024069420A2 (en) | 2022-09-29 | 2024-04-04 | Pfizer Inc. | Immunogenic compositions comprising an rsv f protein trimer |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2970398T3 (da) | 2013-03-13 | 2024-08-05 | Us Health | Præfusions-rsv-f-proteiner og anvendelse deraf |
| AU2014100888A4 (en) * | 2014-08-07 | 2014-09-11 | Novartis Ag | Virus clearance and protein purification methods |
| US20180273909A1 (en) * | 2015-09-22 | 2018-09-27 | Sanofi Pasteur Biologics, Llc | Purification of respiratory syncytial virus |
| AU2016379097C1 (en) | 2015-12-23 | 2021-04-08 | Pfizer Inc. | RSV F protein mutants |
| EA202190136A1 (ru) * | 2018-07-31 | 2021-05-14 | Глаксосмитклайн Байолоджикалс Са | Способ очистки антигена |
-
2021
- 2021-07-21 JP JP2021120073A patent/JP2022023813A/ja active Pending
- 2021-07-22 EP EP21749306.3A patent/EP4188414A1/en active Pending
- 2021-07-22 IL IL299808A patent/IL299808A/en unknown
- 2021-07-22 WO PCT/IB2021/056629 patent/WO2022023895A1/en active Application Filing
- 2021-07-22 CA CA3190141A patent/CA3190141A1/en active Pending
- 2021-07-22 AU AU2021315047A patent/AU2021315047A1/en active Pending
- 2021-07-22 CN CN202180052979.5A patent/CN116194464A/zh active Pending
- 2021-07-22 BR BR112023000638A patent/BR112023000638A2/pt unknown
- 2021-07-22 MX MX2023001192A patent/MX2023001192A/es unknown
- 2021-07-22 US US18/006,800 patent/US20230287041A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4188414A1 (en) | 2023-06-07 |
| BR112023000638A2 (pt) | 2023-01-31 |
| WO2022023895A1 (en) | 2022-02-03 |
| AU2021315047A1 (en) | 2023-03-02 |
| JP2022023813A (ja) | 2022-02-08 |
| IL299808A (en) | 2023-03-01 |
| US20230287041A1 (en) | 2023-09-14 |
| CA3190141A1 (en) | 2022-02-03 |
| CN116194464A (zh) | 2023-05-30 |
| AU2021315047A8 (en) | 2023-03-23 |
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