BR112023000638A2 - Aperfeiçoamentos em soluções de lavagem para a cromatografia de troca de ânions em um método de purificação de proteínas de rsv recombinantemente produzidas - Google Patents

Aperfeiçoamentos em soluções de lavagem para a cromatografia de troca de ânions em um método de purificação de proteínas de rsv recombinantemente produzidas

Info

Publication number
BR112023000638A2
BR112023000638A2 BR112023000638A BR112023000638A BR112023000638A2 BR 112023000638 A2 BR112023000638 A2 BR 112023000638A2 BR 112023000638 A BR112023000638 A BR 112023000638A BR 112023000638 A BR112023000638 A BR 112023000638A BR 112023000638 A2 BR112023000638 A2 BR 112023000638A2
Authority
BR
Brazil
Prior art keywords
anion exchange
exchange chromatography
recombinantly produced
washing solutions
purification method
Prior art date
Application number
BR112023000638A
Other languages
English (en)
Inventor
Ann Kublbeck Jill
Pettaway Alexandra
Richard Salm Jeffrey
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer filed Critical Pfizer
Publication of BR112023000638A2 publication Critical patent/BR112023000638A2/pt

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011Details
    • C12N2760/18011Paramyxoviridae
    • C12N2760/18511Pneumovirus, e.g. human respiratory syncytial virus
    • C12N2760/18522New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011Details
    • C12N2760/18011Paramyxoviridae
    • C12N2760/18511Pneumovirus, e.g. human respiratory syncytial virus
    • C12N2760/18551Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

APERFEIÇOAMENTOS EM SOLUÇÕES DE LAVAGEM PARA A CROMATOGRAFIA DE TROCA DE ÂNIONS EM UM MÉTODO DE PURIFICAÇÃO DE PROTEÍNAS DE RSV RECOMBINANTEMENTE PRODUZIDAS. A presente invenção refere-se a um método de purificação de uma proteína de RSV, em que uma solução de carga compreendendo a proteína de RSV é posta em contato com um meio de cromatografia de troca de ânions, pelo que a proteína de RSV se liga ao meio de cromatografia de troca de ânions, o meio de cromatografia de troca de ânions é lavado com pelo menos uma solução de lavagem e a proteína de RSV é eluída a partir do meio de cromatografia de troca de ânions. De acordo com a invenção, a solução de lavagem tem um pH entre 3,0 e 6,5, pelo que a remoção de proteínas de célula hospedeira é melhorada. A invenção refere-se também a um produto farmacêutico incluindo uma proteína de RSV purificada por tal método.
BR112023000638A 2020-07-27 2021-07-22 Aperfeiçoamentos em soluções de lavagem para a cromatografia de troca de ânions em um método de purificação de proteínas de rsv recombinantemente produzidas BR112023000638A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063056949P 2020-07-27 2020-07-27
PCT/IB2021/056629 WO2022023895A1 (en) 2020-07-27 2021-07-22 Improvements to wash solutions for anion exchange chromatography in a method of purification of recombinantly-produced rsv proteins

Publications (1)

Publication Number Publication Date
BR112023000638A2 true BR112023000638A2 (pt) 2023-01-31

Family

ID=77168315

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023000638A BR112023000638A2 (pt) 2020-07-27 2021-07-22 Aperfeiçoamentos em soluções de lavagem para a cromatografia de troca de ânions em um método de purificação de proteínas de rsv recombinantemente produzidas

Country Status (10)

Country Link
US (1) US20230287041A1 (pt)
EP (1) EP4188414A1 (pt)
JP (1) JP2022023813A (pt)
CN (1) CN116194464A (pt)
AU (1) AU2021315047A1 (pt)
BR (1) BR112023000638A2 (pt)
CA (1) CA3190141A1 (pt)
IL (1) IL299808A (pt)
MX (1) MX2023001192A (pt)
WO (1) WO2022023895A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024069420A2 (en) 2022-09-29 2024-04-04 Pfizer Inc. Immunogenic compositions comprising an rsv f protein trimer

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014160463A1 (en) 2013-03-13 2014-10-02 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Prefusion rsv f proteins and their use
AU2014100888A4 (en) * 2014-08-07 2014-09-11 Novartis Ag Virus clearance and protein purification methods
EP3353193A4 (en) * 2015-09-22 2019-04-03 Sanofi Pasteur Biologics, LLC CLEANING OF THE RESPIRATORY SYNZYTIAL VIRUS
RU2723039C2 (ru) 2015-12-23 2020-06-08 Пфайзер Инк. Мутанты белка f rsv
WO2020026147A1 (en) * 2018-07-31 2020-02-06 Glaxosmithkline Biologicals Sa Antigen purification method

Also Published As

Publication number Publication date
WO2022023895A1 (en) 2022-02-03
CA3190141A1 (en) 2022-02-03
AU2021315047A1 (en) 2023-03-02
CN116194464A (zh) 2023-05-30
IL299808A (en) 2023-03-01
AU2021315047A8 (en) 2023-03-23
MX2023001192A (es) 2023-02-22
JP2022023813A (ja) 2022-02-08
EP4188414A1 (en) 2023-06-07
US20230287041A1 (en) 2023-09-14

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Legal Events

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B25G Requested change of headquarter approved

Owner name: PFIZER INC. (US)