BR112023000638A2 - Aperfeiçoamentos em soluções de lavagem para a cromatografia de troca de ânions em um método de purificação de proteínas de rsv recombinantemente produzidas - Google Patents
Aperfeiçoamentos em soluções de lavagem para a cromatografia de troca de ânions em um método de purificação de proteínas de rsv recombinantemente produzidasInfo
- Publication number
- BR112023000638A2 BR112023000638A2 BR112023000638A BR112023000638A BR112023000638A2 BR 112023000638 A2 BR112023000638 A2 BR 112023000638A2 BR 112023000638 A BR112023000638 A BR 112023000638A BR 112023000638 A BR112023000638 A BR 112023000638A BR 112023000638 A2 BR112023000638 A2 BR 112023000638A2
- Authority
- BR
- Brazil
- Prior art keywords
- anion exchange
- exchange chromatography
- recombinantly produced
- washing solutions
- purification method
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18511—Pneumovirus, e.g. human respiratory syncytial virus
- C12N2760/18522—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18511—Pneumovirus, e.g. human respiratory syncytial virus
- C12N2760/18551—Methods of production or purification of viral material
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
APERFEIÇOAMENTOS EM SOLUÇÕES DE LAVAGEM PARA A CROMATOGRAFIA DE TROCA DE ÂNIONS EM UM MÉTODO DE PURIFICAÇÃO DE PROTEÍNAS DE RSV RECOMBINANTEMENTE PRODUZIDAS. A presente invenção refere-se a um método de purificação de uma proteína de RSV, em que uma solução de carga compreendendo a proteína de RSV é posta em contato com um meio de cromatografia de troca de ânions, pelo que a proteína de RSV se liga ao meio de cromatografia de troca de ânions, o meio de cromatografia de troca de ânions é lavado com pelo menos uma solução de lavagem e a proteína de RSV é eluída a partir do meio de cromatografia de troca de ânions. De acordo com a invenção, a solução de lavagem tem um pH entre 3,0 e 6,5, pelo que a remoção de proteínas de célula hospedeira é melhorada. A invenção refere-se também a um produto farmacêutico incluindo uma proteína de RSV purificada por tal método.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202063056949P | 2020-07-27 | 2020-07-27 | |
PCT/IB2021/056629 WO2022023895A1 (en) | 2020-07-27 | 2021-07-22 | Improvements to wash solutions for anion exchange chromatography in a method of purification of recombinantly-produced rsv proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112023000638A2 true BR112023000638A2 (pt) | 2023-01-31 |
Family
ID=77168315
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112023000638A BR112023000638A2 (pt) | 2020-07-27 | 2021-07-22 | Aperfeiçoamentos em soluções de lavagem para a cromatografia de troca de ânions em um método de purificação de proteínas de rsv recombinantemente produzidas |
Country Status (10)
Country | Link |
---|---|
US (1) | US20230287041A1 (pt) |
EP (1) | EP4188414A1 (pt) |
JP (1) | JP2022023813A (pt) |
CN (1) | CN116194464A (pt) |
AU (1) | AU2021315047A1 (pt) |
BR (1) | BR112023000638A2 (pt) |
CA (1) | CA3190141A1 (pt) |
IL (1) | IL299808A (pt) |
MX (1) | MX2023001192A (pt) |
WO (1) | WO2022023895A1 (pt) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2024069420A2 (en) | 2022-09-29 | 2024-04-04 | Pfizer Inc. | Immunogenic compositions comprising an rsv f protein trimer |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014160463A1 (en) | 2013-03-13 | 2014-10-02 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Prefusion rsv f proteins and their use |
AU2014100888A4 (en) * | 2014-08-07 | 2014-09-11 | Novartis Ag | Virus clearance and protein purification methods |
EP3353193A4 (en) * | 2015-09-22 | 2019-04-03 | Sanofi Pasteur Biologics, LLC | CLEANING OF THE RESPIRATORY SYNZYTIAL VIRUS |
RU2723039C2 (ru) | 2015-12-23 | 2020-06-08 | Пфайзер Инк. | Мутанты белка f rsv |
WO2020026147A1 (en) * | 2018-07-31 | 2020-02-06 | Glaxosmithkline Biologicals Sa | Antigen purification method |
-
2021
- 2021-07-21 JP JP2021120073A patent/JP2022023813A/ja active Pending
- 2021-07-22 BR BR112023000638A patent/BR112023000638A2/pt unknown
- 2021-07-22 CA CA3190141A patent/CA3190141A1/en active Pending
- 2021-07-22 CN CN202180052979.5A patent/CN116194464A/zh active Pending
- 2021-07-22 MX MX2023001192A patent/MX2023001192A/es unknown
- 2021-07-22 AU AU2021315047A patent/AU2021315047A1/en active Pending
- 2021-07-22 EP EP21749306.3A patent/EP4188414A1/en active Pending
- 2021-07-22 WO PCT/IB2021/056629 patent/WO2022023895A1/en active Application Filing
- 2021-07-22 US US18/006,800 patent/US20230287041A1/en active Pending
- 2021-07-22 IL IL299808A patent/IL299808A/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO2022023895A1 (en) | 2022-02-03 |
CA3190141A1 (en) | 2022-02-03 |
AU2021315047A1 (en) | 2023-03-02 |
CN116194464A (zh) | 2023-05-30 |
IL299808A (en) | 2023-03-01 |
AU2021315047A8 (en) | 2023-03-23 |
MX2023001192A (es) | 2023-02-22 |
JP2022023813A (ja) | 2022-02-08 |
EP4188414A1 (en) | 2023-06-07 |
US20230287041A1 (en) | 2023-09-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B25G | Requested change of headquarter approved |
Owner name: PFIZER INC. (US) |