MX2022009416A - Uso de cromatografía líquida y espectrometría de masas para caracterizar oligonucleótidos. - Google Patents

Uso de cromatografía líquida y espectrometría de masas para caracterizar oligonucleótidos.

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Publication number
MX2022009416A
MX2022009416A MX2022009416A MX2022009416A MX2022009416A MX 2022009416 A MX2022009416 A MX 2022009416A MX 2022009416 A MX2022009416 A MX 2022009416A MX 2022009416 A MX2022009416 A MX 2022009416A MX 2022009416 A MX2022009416 A MX 2022009416A
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Mexico
Prior art keywords
oligonucleotides
mass spectrometry
liquid chromatography
characterize
characterizing
Prior art date
Application number
MX2022009416A
Other languages
English (en)
Inventor
Ning Li
Ming Huang
Xiaobin Xu
Haibo Qiu
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Regeneron Pharma
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Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2022009416A publication Critical patent/MX2022009416A/es

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/107—Modifications characterised by incorporating a peptide nucleic acid
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/113—Modifications characterised by incorporating modified backbone
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/125—Modifications characterised by incorporating agents resulting in resistance to degradation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/207—Modifications characterised by siRNA, miRNA
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/15—Gradients
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/137—Chromatographic separation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/627—Detection means characterised by use of a special device being a mass spectrometer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La descripción proporciona métodos para caracterizar una muestra de oligonucleótidos de interés mediante el uso de cromatografía líquida y espectrometría de masas.
MX2022009416A 2020-01-31 2021-01-29 Uso de cromatografía líquida y espectrometría de masas para caracterizar oligonucleótidos. MX2022009416A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202062968368P 2020-01-31 2020-01-31
PCT/US2021/015697 WO2021155139A1 (en) 2020-01-31 2021-01-29 Use of liquid chromatography and mass spectrometry to characterize oligonucleotides

Publications (1)

Publication Number Publication Date
MX2022009416A true MX2022009416A (es) 2022-10-21

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Country Status (11)

Country Link
US (1) US20210239663A1 (es)
EP (1) EP4097256A1 (es)
JP (1) JP2023512075A (es)
KR (1) KR20220136376A (es)
CN (1) CN115552034A (es)
AU (1) AU2021212195A1 (es)
BR (1) BR112022014992A2 (es)
CA (1) CA3165815A1 (es)
IL (1) IL295038A (es)
MX (1) MX2022009416A (es)
WO (1) WO2021155139A1 (es)

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EP4114545A1 (en) * 2020-03-06 2023-01-11 Waters Technologies Corporation Purification and isolation of synthetic oligonucleotides using hydrophilic-interaction liquid chromatography
CN114577954B (zh) * 2022-05-09 2022-08-02 北京生物制品研究所有限责任公司 检测吸附型疫苗中CpG ODN含量的方法
WO2023218329A1 (en) * 2022-05-10 2023-11-16 Waters Technologies Corporation Method of analysis of polynucleotides by restricted access reversed phase chromatography
CN116656781B (zh) * 2023-07-07 2024-04-26 中国药科大学 一种用于检测反义寡核苷酸类药物的荧光探针及检测方法
CN117269402B (zh) * 2023-11-20 2024-02-23 中国医学科学院北京协和医院 一种定量检测人脑脊液中诺西那生的液相质谱联用方法
CN117250301B (zh) * 2023-11-20 2024-02-09 中国医学科学院北京协和医院 一种定量检测人血浆中诺西那生的液相质谱联用方法

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Publication number Publication date
BR112022014992A2 (pt) 2022-10-11
EP4097256A1 (en) 2022-12-07
IL295038A (en) 2022-09-01
JP2023512075A (ja) 2023-03-23
CA3165815A1 (en) 2021-08-05
CN115552034A (zh) 2022-12-30
AU2021212195A1 (en) 2022-09-15
WO2021155139A1 (en) 2021-08-05
KR20220136376A (ko) 2022-10-07
US20210239663A1 (en) 2021-08-05

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